Layer-by-Layer Investigation of Ultrastructures and Biomechanics of Human Cornea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical
2.2. Sample Acquisition and Preparation
2.3. Atomic Force Microscopy (AFM) Ultrastructural and Nanomechanical Experiments
2.3.1. AFM Setup
2.3.2. Ultrastructure Analysis
Diameter Analysis
2.3.3. Nanomechanical Analysis
2.3.4. Statistical Analysis
3. Results
3.1. Epithelial Layer
Epithelial Basement Membrane
3.2. Bowman’s Layer
3.3. Stroma
3.4. Endothelium
3.5. Biophysical Properties of Layers
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rates, E.R.D.; Almeida, C.D.; Costa, E.d.P.F.; Farias, R.J.d.M.; Santos-Oliveira, R.; Alencar, L.M.R. Layer-by-Layer Investigation of Ultrastructures and Biomechanics of Human Cornea. Int. J. Mol. Sci. 2022, 23, 7833. https://doi.org/10.3390/ijms23147833
Rates ERD, Almeida CD, Costa EdPF, Farias RJdM, Santos-Oliveira R, Alencar LMR. Layer-by-Layer Investigation of Ultrastructures and Biomechanics of Human Cornea. International Journal of Molecular Sciences. 2022; 23(14):7833. https://doi.org/10.3390/ijms23147833
Chicago/Turabian StyleRates, Erick Rafael Dias, Charles Duarte Almeida, Elaine de Paula Fiod Costa, Roberta Jansen de Mello Farias, Ralph Santos-Oliveira, and Luciana Magalhães Rebelo Alencar. 2022. "Layer-by-Layer Investigation of Ultrastructures and Biomechanics of Human Cornea" International Journal of Molecular Sciences 23, no. 14: 7833. https://doi.org/10.3390/ijms23147833