Identification, Molecular Characteristic, and Expression Analysis of PIFs Related to Chlorophyll Metabolism in Tea Plant (Camellia sinensis)
Abstract
:1. Introduction
2. Results
2.1. Identification of CsPIF Family Members in Tea Plants
2.2. Amino Acid Sequence and Phylogenetic Analysis of CsPIF Proteins
2.3. Gene Structural Analysis and Motif Analysis of CsPIFs
2.4. Expression Patterns and Correlation Analysis of CsPIFs and Structural Genes Related Chlorophyll Metabolism in Different Leaf Color Tea Cultivars
2.5. Protein–Protein Interaction Network of CsPIFs and Binding Elements in Promoters of Chlorophyll Metabolism-Related Gene
2.6. Subcellular Localization and Transcriptional Activation of CsPIF3b
2.7. CsPIF3b Positively Regulate the Expression of CsHEMA and CsPOR
3. Discussion
3.1. Identification and Characterization of PIF Genes in Tea Plants
3.2. CsPIF Correlated with the Expression of Chlorophyll Metabolism Genes
3.3. CsPIF Involved in the Transcriptional Regulation of Chlorophyll Metabolism
4. Materials and Methods
4.1. Plant Materials
4.2. Identification of PIFs Transcriptional Factors
4.3. Sequence Alignments and Phylogenetic Analysis
4.4. Gene Structure and Conserved Motifs Analysis
4.5. qRT-PCR and Correlation Analysis of CsPIFs and Structural Genes Related Chlorophyll Metabolism
4.6. Analysis of the Protein–Protein Interaction (PPI) Network of PIF TFs and Promoter Regions of Structural Genes Related to Chlorophyll Metabolism
4.7. Subcellular Localization and Transcriptional Activation Assays of PIFs
4.8. Dual-Luciferase Transient Transfection Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
PIFs | phytochrome-interacting factors |
Cs | Camellia sinensis |
ALA | 5-aminolevulinic acid |
APB | domain Active phytochrome B-binding domain |
APA | domain Active phytochrome A-binding domain |
qRT-PCR | quantitative real-time polymerase chain reaction |
HEMA | glutamyl-tRNA reductase |
HEME | uroporphyrinogen decarboxylase |
CHLI | Mg-protoporphyrin IX chelatase subunit ChlI |
POR | protochlorophyllide oxidoreductase |
CAO | chlorophyll a oxygenase |
SGR | STAY-GREEN |
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Gene Name | Gene ID | CDS Length | Number of Amino Acid | Molecular Weight | Protein Isoelectric Point | Instability Index | Grand Average of Hydropathicity | Predicted Subcellular Localization |
---|---|---|---|---|---|---|---|---|
(bp) | (KD) | (GRAVY) | ||||||
CsPIF1 | TEA006532 | 1716 | 571 | 62.64 | 5.96 | 60.45 | −0.546 | Nucleus |
CsPIF3a | TEA033210 | 1884 | 723 | 77.34 | 5.88 | 50.32 | −0.554 | Nucleus |
CsPIF3b | TEA007077 | 2172 | 627 | 68.2 | 5.93 | 67.13 | −0.634 | Nucleus |
CsPIF7a | TEA025875 | 1272 | 423 | 46.76 | 8.17 | 73.85 | −0.703 | Nucleus |
CsPIF7b | TEA011633 | 1440 | 479 | 53.01 | 9.26 | 61 | −0.558 | Nucleus |
CsPIF8a | TEA032260 | 1251 | 416 | 44.84 | 8.3 | 43.83 | −0.332 | Nucleus |
CsPIF8b | TEA023842 | 1290 | 429 | 47.17 | 8.7 | 52.96 | −0.592 | Nucleus |
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Zhang, X.; Xiong, L.; Luo, Y.; Wen, B.; Wang, K.; Liu, Z.; Huang, J.-a.; Li, J. Identification, Molecular Characteristic, and Expression Analysis of PIFs Related to Chlorophyll Metabolism in Tea Plant (Camellia sinensis). Int. J. Mol. Sci. 2021, 22, 10949. https://doi.org/10.3390/ijms222010949
Zhang X, Xiong L, Luo Y, Wen B, Wang K, Liu Z, Huang J-a, Li J. Identification, Molecular Characteristic, and Expression Analysis of PIFs Related to Chlorophyll Metabolism in Tea Plant (Camellia sinensis). International Journal of Molecular Sciences. 2021; 22(20):10949. https://doi.org/10.3390/ijms222010949
Chicago/Turabian StyleZhang, Xiangna, Ligui Xiong, Yong Luo, Beibei Wen, Kunbo Wang, Zhonghua Liu, Jian-an Huang, and Juan Li. 2021. "Identification, Molecular Characteristic, and Expression Analysis of PIFs Related to Chlorophyll Metabolism in Tea Plant (Camellia sinensis)" International Journal of Molecular Sciences 22, no. 20: 10949. https://doi.org/10.3390/ijms222010949