2nd Edition of Advances in Vaccine Biomanufacturing Processes

A special issue of Vaccines (ISSN 2076-393X).

Deadline for manuscript submissions: closed (30 June 2023) | Viewed by 1825

Special Issue Editor


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Guest Editor
Department of Chemical Engineering, Polytechnique Montréal, Montréal, QC, Canada
Interests: recombinant proteins; viral vectors; vaccines; animal cell culture; bioprocess development, optimization and control
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Special Issue Information

Dear Colleagues.

Continuous improvement of vaccine manufacturing processes is essential to meeting the needs of the growing global market. In this context, further research and development efforts are still needed to address the critical process and analytical challenges faced by biomanufacturers to speed up the production of safe, potent and cost-effective vaccines. The aim of this Special Issue is to present significant advances made in bioprocessing for the robust, rapid, flexible and cost-efficient manufacturing of vaccines. Contributions on a range of themes are welcome, including process intensification of upstream and downstream operations to improve productivity and increase product yield and purity, the development and application of new process analytical technologies to support process development and analyses of product quality attributes, the design of novel monitoring tools and control strategies to ensure process consistency and performance, the application of new high-throughput technologies and Quality-by-Design approaches for vaccine manufacturing, and devising new cell line platforms, innovative bioreactor designs and downstream processing techniques. Since rational process development and optimization are highly dependent on a good understanding of the complex production steps and interactions between cells and viruses, contributions related to the modeling of cell culture kinetics, as well as the application of systems biology and metabolic engineering approaches, are also welcome.

Prof. Dr. Olivier Henry
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Vaccines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • vaccine manufacturing
  • process intensification
  • upstream process development
  • downstream processing
  • process analytical technologies

Published Papers (1 paper)

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Research

11 pages, 3443 KiB  
Article
Evaluation of Virus-Free Manufacture of Recombinant Proteins Using CRISPR-Mediated Gene Disruption in Baculovirus-Infected Insect Cells
by Mark R. Bruder and Marc G. Aucoin
Vaccines 2023, 11(2), 225; https://doi.org/10.3390/vaccines11020225 - 19 Jan 2023
Cited by 2 | Viewed by 1578
Abstract
The manufacture and downstream processing of virus-like particles (VLPs) using the baculovirus expression vector system (BEVS) is complicated by the presence of large concentrations of baculovirus particles, which are similar in size and density to VLPs, and consequently are difficult to separate. To [...] Read more.
The manufacture and downstream processing of virus-like particles (VLPs) using the baculovirus expression vector system (BEVS) is complicated by the presence of large concentrations of baculovirus particles, which are similar in size and density to VLPs, and consequently are difficult to separate. To reduce the burden of downstream processing, CRISPR-Cas9 technology was used to introduce insertion-deletion (indel) mutations within the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) gp64 open reading frame, which encodes the major envelope protein of AcMNPV. After comfirming the site-specific targeting of gp64 leading to reduced budded virus (BV) release, the gag gene of human immunodeficiency virus type 1 was expressed to produce Gag VLPs. This approach was effective for producing VLPs using the BEVS whilst simultaneously obstructing BV release. Full article
(This article belongs to the Special Issue 2nd Edition of Advances in Vaccine Biomanufacturing Processes)
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