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Collection：Fluorescent Biosensors

A topical collection in Sensors (ISSN 1424-8220). This collection belongs to the section "Biosensors".

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Editor

Prof. Dr. Christopher Bystroff

E-Mail Website
Collection Editor
Renssleaer Polytechnic Institute, Department of Biological Sciences, Department of Computer Science, Troy, NY 12180, USA
Interests: protein design; protein folding; folding pathways; bioinformatics; vaccine design
Special Issues, Collections and Topics in MDPI journals

Topical Collection Information

Dear Colleagues,

Biological macromolecules provide a ready toolkit for specific binding and easy manufacture. Proteins can fold to specific structures with unlimited diversity and even catalytic activity. DNA recognizes its complement at vanishingly small concentrations, and RNA can be engineered to switch states in the presence of an analyte compound or another macromolecule. Biomolecules may be genetically encoded, and expressed in living things. They may be screened from libraries or subjected to in vitro evolution. Fluorescence may be added to biomolecules chemically or generated autocatalytically using natural or engineered fluorescent proteins. Fluorescent biosensors are biological macromolecules that produce, modify or quench a fluorescent signal in response to the presence of a specific analyte.

The aim of this Topical Collection is to feature novel fluorescent biosensors, their design, their mechanism of action, their production, and their applications. We invite original contributions on topics related to fluorescent biosensor technology, methodology, and applications, including, but not limited to:

Förster resonance energy transfer (FRET)

Fluorescence activated cell sorting (FACS)

Fluorescent antibody applications

Designed/engineered fluorescent proteins

Bioluminescent sensors

Chemiluminescent bioconjugate sensors

Protein/nucleotide conjugates

Biosensor arrays

Biosensor fibers

Cell-based fluorescent biosensing

RNA-based fluorescent biosensing

DNA-based fluorescent biosensing

Protein-based fluorescent biosensing

Therapeutic/diagnostic biosensors

Quantum dot bioconjugates

Prof. Dr. Christopher Bystroff
Collection Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

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2024

Jump to: 2022

19 pages, 7508 KiB  
Article
Susceptibility of the Different Oxygen-Sensing Probes to Interferences in Respirometric Bacterial Assays with Complex Media
by Chiara Zanetti, Liang Li, Rafael Di Lazaro Gaspar, Elisa Santovito, Sophia Elisseeva, Stuart G. Collins, Anita R. Maguire and Dmitri B. Papkovsky
Sensors 2024, 24(1), 267; https://doi.org/10.3390/s24010267 - 02 Jan 2024
Viewed by 851
Abstract
Respirometric microbial assays are gaining popularity, but their uptake is limited by the availability of optimal O2 sensing materials and the challenge of validating assays with complex real samples. We conducted a comparative evaluation of four different O2-sensing probes based [...] Read more.
Respirometric microbial assays are gaining popularity, but their uptake is limited by the availability of optimal O2 sensing materials and the challenge of validating assays with complex real samples. We conducted a comparative evaluation of four different O2-sensing probes based on Pt-porphyrin phosphors in respirometric bacterial assays performed on standard time-resolved fluorescence reader. The macromolecular MitoXpress, nanoparticle NanO2 and small molecule PtGlc4 and PtPEG4 probes were assessed with E. coli cells in five growth media: nutrient broth (NB), McConkey (MC), Rapid Coliform ChromoSelect (RCC), M-Lauryl lauryl sulfate (MLS), and Minerals-Modified Glutamate (MMG) media. Respiration profiles of the cells were recorded and analyzed, along with densitometry profiles and quenching studies of individual media components. This revealed several limiting factors and interferences impacting assay performance, which include probe quenched lifetime, instrument temporal resolution, inner filter effects (mainly by indicator dyes), probe binding to lipophilic components, and dynamic and static quenching by media components. The study allowed for the ranking of the probes based on their ruggedness, resilience to interferences and overall performance in respirometric bacterial assays. The ‘shielded’ probe NanO2 outperformed the established MitoXpress probe and the small molecule probes PtGlc4 and PtPEG4. Full article
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2022

Jump to: 2024

21 pages, 5425 KiB  
Article
Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts
by Anggy Lusanna Gutiérrez-Ortiz, Veronica Vida, Matjaž Peterka, Jasmina Tušar, Federico Berti, Luciano Navarini and Cristina Forzato
Sensors 2022, 22(24), 9874; https://doi.org/10.3390/s22249874 - 15 Dec 2022
Cited by 1 | Viewed by 1262
Abstract
Green coffee beans are particularly rich in chlorogenic acids (CGAs), and their identification and quantification are usually performed by HPLC, coupled with mass spectrometry (LC-MS). Although there are a few examples of molecularly imprinted polymers (MIPs) for chlorogenic acid (5-CQA) recognition present in [...] Read more.
Green coffee beans are particularly rich in chlorogenic acids (CGAs), and their identification and quantification are usually performed by HPLC, coupled with mass spectrometry (LC-MS). Although there are a few examples of molecularly imprinted polymers (MIPs) for chlorogenic acid (5-CQA) recognition present in the literature, none of them are based on optical fluorescence, which is very interesting given its great sensitivity. In the present manuscript, fluorescent polymeric imprinted nanoparticles were synthetized following the non-covalent approach using hydrogenated 5-O-caffeoylquinic acid (H-5-CQA) as the template. The capability of the polymer to bind 5-CQA was evaluated by HPLC and fluorescence. A real sample of coffee extract was also analyzed to verify the selectivity of the polymer. Polymer fMIP01, containing 4-vinylpyridine and a naphtalimide derivative as monomers, showed a good response to the fluorescence quenching in the range 39 μM–80 mM. In the real sample, fMIP01 was able to selectively bind 5-CQA, while caffeine was not recognized. To demonstrate this, there is a promising system that can be exploited in the design of an optical sensor for 5-CQA detection. Polymer fMIP01 was immobilized by physical entrapment on a functionalized glass surface, showing a quenching of fluorescence with an increase of the CGA concentration between 156 μM and 40 mM. Full article
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