Emerging Spectroscopic Detection and Analytical Methods for Plant and Animal Analysis

A special issue of Methods and Protocols (ISSN 2409-9279). This special issue belongs to the section "Biochemical and Chemical Analysis & Synthesis".

Deadline for manuscript submissions: closed (31 May 2022) | Viewed by 3657

Special Issue Editor

Special Issue Information

Dear Colleagues,

Advances in optics, physics, spectroscopy, and mathematics have prompted a wide range of novel applications in different fields of biology and chemistry. In particular, the utilization of near-infrared spectroscopy (NIR), mid-infrared spectroscopy (MIR), Raman spectroscopy, and hyperspectral imaging has been the backbone of the so-called high-throughput analytical methods. The advances in machine learning, new algorithms, etc. have boosted the incorporation of these techniques in many fields of research (phenotyping, functionality, etc.).

In this Special Issue titled “Emerging Spectroscopic Detection and Analytical Methods for Plant and Animal Analysis”, we welcome original research articles, review articles, innovative protocols, and reports on these topics. This Special Issue seeks high-quality works and topics focusing on the latest novel applications of NIR, MIR, and Raman spectroscopy, as well as hyperspectral imaging.

Prof. Dr. Daniel Cozzolino
Guest Editor

Manuscript Submission Information

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Keywords

  • near-infrared
  • hyperspectral
  • image
  • mid-infrared
  • Raman
  • big data
  • multivariate data analysis
  • machine learning

Published Papers (1 paper)

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Research

12 pages, 1380 KiB  
Article
Near-Infrared Spectroscopy (NIRS) as a Method for Biological Sex Discrimination in the Endangered Houston Toad (Anaxyrus houstonensis)
by Li-Dunn Chen, Mariana Santos-Rivera, Isabella J. Burger, Andrew J. Kouba, Diane M. Barber and Carrie K. Vance
Methods Protoc. 2022, 5(1), 4; https://doi.org/10.3390/mps5010004 - 30 Dec 2021
Cited by 3 | Viewed by 2812
Abstract
Biological sex is one of the more critically important physiological parameters needed for managing threatened animal species because it is crucial for informing several of the management decisions surrounding conservation breeding programs. Near-infrared spectroscopy (NIRS) is a non-invasive technology that has been recently [...] Read more.
Biological sex is one of the more critically important physiological parameters needed for managing threatened animal species because it is crucial for informing several of the management decisions surrounding conservation breeding programs. Near-infrared spectroscopy (NIRS) is a non-invasive technology that has been recently applied in the field of wildlife science to evaluate various aspects of animal physiology and may have potential as an in vivo technique for determining biological sex in live amphibian species. This study investigated whether NIRS could be used as a rapid and non-invasive method for discriminating biological sex in the endangered Houston toad (Anaxyrus houstonensis). NIR spectra (N = 396) were collected from live A. houstonensis individuals (N = 132), and distinct spectral patterns between males and females were identified using chemometrics. Linear discriminant analysis (PCA-LDA) classified the spectra from each biological sex with accuracy ≥ 98% in the calibration and internal validation datasets and 94% in the external validation process. Through the use of NIRS, we have determined that unique spectral signatures can be holistically captured in the skin of male and female anurans, bringing to light the possibility of further application of this technique for juveniles and sexually monomorphic species, whose sex designation is important for breeding-related decisions. Full article
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