Cellular Reprogramming and Tissue Repair
A special issue of Methods and Protocols (ISSN 2409-9279).
Deadline for manuscript submissions: closed (22 June 2022) | Viewed by 7093
Special Issue Editor
Interests: cellular reprogramming; pluripotent stem cells
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
In 2006, Drs. Yamanaka and Takahashi reprogrammed mouse fibroblasts into pluripotent stem cells (PSCs) via induction by overexpression of a set of four transcription factors, Oct4, Sox2, Klf4, and c-Myc. Factor-induced PSCs (iPSCs) have since been generated from human cells of different cell types, and from many other species. Factor reprogramming has generated other functional cells as well, including induced cardiomyoctyes, induced neurons, induced beta cells, and induced blood cells, among others. This new technology opens up a new field of research—cellular reprogramming. In theory, we can now convert any cell type into another cell type of interest through epigenetic reprogramming. The means of reprogramming is no longer limited to overexpression of transcription factors. Almost all approaches have been used in reprogramming, for example, protein reprogramming, mRNA reprogramming, chemical reprogramming, microRNA reprogramming, sendai viral reprogramming, episomal reprogramming, CRISPR/activator reprogramming, reprograming with replicating viral RNA, and others. Cell fate conversion can be achieved not only in vitro, but also in living animals by delivering reprogramming factors into the target tissues. Although factor reprogramming dominates the field, the conventional technology of reprogramming by somatic cell nuclear transfer (SCNT) is still a valuable tool in both biotechnology and research. For example, SCNT is still critical in the cloning of large animals such as pigs. Cellular reprogramming is a work of art, and detailed and standardized methods and protocols are needed. This issue aims at compiling various proven bench protocols in cellular reprogramming into a single volume.
Dr. Kejin Hu
Guest Editor
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