Biophotonic Techniques in Biological Molecules, Cells and Tissues: New Advances and Future Trends

A special issue of Biomolecules (ISSN 2218-273X). This special issue belongs to the section "Biological and Bio- Materials".

Deadline for manuscript submissions: 31 May 2024 | Viewed by 1468

Special Issue Editor

Department of Cell Biology and Anatomy, School of Medicine Columbia, University of South Carolina, Columbia, SC, USA
Interests: congenital vascular malformations; laser therapy; optogenetics; extracellular vesicles

Special Issue Information

Dear Colleagues,

The rapid development and advances in biophotonic-based technologies have led to a new era of our understanding of the unique “optical aspect” of biological molecules, cells, and tissues during their photonic interactions.

The focus of this Special Issue of Biomolecules will be the most recent advances and applications of light-based technologies, involving basic, translational, and clinical studies. Research and review articles that present novelties or overviews related to biophotonics are welcome. Particularly, studies from researchers and physicians on the following topics would be highly appreciated: (1) development and characterization of new photonic materials, such as photosensitizers or fluorophores, or repurposing of existing photosensitizers or fluorophores; (2) biophotonic-based technologies for imaging and diagnosis, involving biofluorescence, OCT, bioluminescence, biophosphorescence, and photoacoustic imaging, etc.; (3) translational and clinical studies that involve photothermal, photodynamic, and laser therapies; (4) optogenetics for functional modulations on cells and tissues; (5) other biophotonic-related biomaterials or techniques.

Dr. Wenbin Tan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biomolecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biophotonics
  • optogenetics
  • lasers
  • diagnosis
  • image
  • therapy

Published Papers (1 paper)

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Research

22 pages, 3831 KiB  
Article
Synthesis and Characterization of a Novel Concentration-Independent Fluorescent Chloride Indicator, ABP-Dextran, Optimized for Extracellular Chloride Measurement
by Kieran P. Normoyle, Kyle P. Lillis and Kevin J. Staley
Biomolecules 2024, 14(1), 77; https://doi.org/10.3390/biom14010077 - 8 Jan 2024
Viewed by 1171
Abstract
GABA, the primary inhibitory neurotransmitter, stimulates GABAA receptors (GABAARs) to increase the chloride conductance of the cytosolic membrane. The driving forces for membrane chloride currents are determined by the local differences between intracellular and extracellular chloride concentrations (Cli and Clo, respectively). While several [...] Read more.
GABA, the primary inhibitory neurotransmitter, stimulates GABAA receptors (GABAARs) to increase the chloride conductance of the cytosolic membrane. The driving forces for membrane chloride currents are determined by the local differences between intracellular and extracellular chloride concentrations (Cli and Clo, respectively). While several strategies exist for the measurement of Cli, the field lacks tools for non-invasive measurement of Clo. We present the design and development of a fluorescent lifetime imaging (FLIM)-compatible small molecule, N(4-aminobutyl)phenanthridiunium (ABP) with the brightness, spectral features, sensitivity to chloride, and selectivity versus other anions to serve as a useful probe of Clo. ABP can be conjugated to dextran to ensure extracellular compartmentalization, and a second chloride-insensitive counter-label can be added for ratiometric imaging. We validate the utility of this novel sensor series in two sensor concentration-independent modes: FLIM or ratiometric intensity-based imaging. Full article
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