Effects of ATP on Time Parameters of Contractility of Rats’ Slow and Fast Skeletal Muscles in Normal and Hypothermic Conditions
Round 1
Reviewer 1 Report
Comments for author File: 
Comments.pdf
Author Response
We thank the Reviewer for the positive comments to our Manuscript.
Author Response File: 
Author Response.docx
Reviewer 2 Report
Interesting data were obtained in the work, but the expediency of performing this work is poorly substantiated. The authors are advised to clarify the relevance of this study.
Author Response
Thank you for your comments.
It is known that usually under physiological conditions P2 receptors are only modulators of cell and system functions increasing or decreasing the effects of the main mediators (acetylcholine, norepinephrine, NO, etc.). However, the role of P2 receptors increases significantly in pathophysiological conditions (hypothermia, stress, inflammation, ischemia, denervation), when they can begin to play a dominant role as a signaling molecule (Burnstock, 2013). Therefore, it is important to assess the involvement of P2 receptors in the regulation of neuromuscular conduction in a comparative aspect - in normothermic and hypothermic conditions. We studied the influence of agonists and antagonists of P2 receptors on the functioning of skeletal muscles in several pathological and extreme situations, which allowed us to identify the mechanisms of functioning of motor units, as well as to advance in understanding the features of movement control in normal and pathological conditions.
Author Response File: Author Response.docx
Reviewer 3 Report
Manuscript ID: muscles-2133043
Effects of ATP on time parameters of contractility of rat slow and
fast skeletal muscles at normal and hypothermic conditions
was the ATP effect dependent on exercise the animal had carried been out before the experiment?
The English should be improved
12/22
Comments for author File: Comments.pdf
Author Response
Reviewer 3:
Effects of ATP on time parameters of contractility of rat slow and fast skeletal muscles at normal and hypothermic conditions was the ATP effect dependent on exercise the animal had carried been out before the experiment?
Our Response:
Thank you for your review of our paper.
All animals were in the same conditions and did not perform any additional loads or exercises. However, this is a very interesting proposal, perhaps in the future we will conduct a pilot series of experiments to compare trained and untrained animals in order to find differences in the effects of ATP on the parameters of contractions.
Reviewer 3:
The English should be improved
Our Response:
Thank you for your comments. We gone through the text of the manuscript several times ourselves and asked one of our colleagues with good English to help us. We have found several misprints and wrong style sentences. We hope that we have corrected the text to the needed standard.
Author Response File: Author Response.docx
