Desialylation of Blood Plasma Lipoproteins by Exogenous Sialidase †
1
Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia
2
Laboratory of Cellular and Molecular Pathology of Cardiovascular System, Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution “Petrovsky National Research Centre of Surgery”, 117418 Moscow, Russia
3
Department of Biology and General Genetics, I.M. Sechenov First Moscow State Medical University (Sechenov University), 105043 Moscow, Russia
*
Author to whom correspondence should be addressed.
†
Presented at the 8th International Electronic Conference on Medicinal Chemistry, 1–30 November 2022; Available online: https://ecmc2022.sciforum.net/ .
Med. Sci. Forum 2022, 14(1), 107; https://doi.org/10.3390/ECMC2022-13310
Published: 1 November 2022
(This article belongs to the Proceedings of The 8th International Electronic Conference on Medicinal Chemistry)
Abstract
:Objective: Sialidases can play an important role in atherosclerosis development due to modification of low-density lipoproteins (LDL). It is known that desialylated LDLs are associated with atherosclerosis development. However, the information about factors leading to desialylation of LDL needs clarifying. The creation of an appropriate model object could help to understand factors related to desialylation of LDL. The aim of this study was to test the possibility to change sialylation of lipoproteins in healthy mice upon an injection of immobilized sialidase. Methods: The control group of C57BL6 mice (n = 48) was treated by a single injection of saline, while the experimental group (n = 48) received Vibrio cholerae sialidase conjugated with mouse IgG. Mice were terminated at fixed periods: before and after a single injection (one to seven days). LDL was isolated from serum by ultracentrifugation. The content of sialic acid was determined according to Warren’s method. Lipids of serum were measured by commercial kits. Results: A significant decrease in LDL sialic acid by 30% was detected up to five days after the sialidase injection. Additionally, serum levels of triglycerides, total cholesterol, and HDL-cholesterol in experimental mice did not differ compared with wild-type control mice. Conclusions: A new approach to study the role of sialidase as a proatherogenic factor in vivo was established.
Supplementary Materials
The following are available online at https://www.mdpi.com/article/10.3390/ECMC2022-13310/s1.
Author Contributions
Conceptualization, D.K. and E.B.; methodology, D.K.; validation, D.K. and E.B.; formal analysis, D.K.; investigation, D.K.; resources, D.K.; data curation, D.K.; writing—original draft preparation, E.B.; writing—review and editing, E.B.; visualization, D.K.; supervision, D.K.; project administration, D.K.; funding acquisition, D.K. All authors have read and agreed to the published version of the manuscript.
Funding
This research was funded by the Russian Science Foundation grant number 20-15-00264.
Conflicts of Interest
The authors declare no conflict of interest.
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MDPI and ACS Style
Bezsonov, E.; Kashirskikh, D. Desialylation of Blood Plasma Lipoproteins by Exogenous Sialidase. Med. Sci. Forum 2022, 14, 107. https://doi.org/10.3390/ECMC2022-13310
AMA Style
Bezsonov E, Kashirskikh D. Desialylation of Blood Plasma Lipoproteins by Exogenous Sialidase. Medical Sciences Forum. 2022; 14(1):107. https://doi.org/10.3390/ECMC2022-13310
Chicago/Turabian StyleBezsonov, Evgeny, and Dmitry Kashirskikh. 2022. "Desialylation of Blood Plasma Lipoproteins by Exogenous Sialidase" Medical Sciences Forum 14, no. 1: 107. https://doi.org/10.3390/ECMC2022-13310
