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Abstract

Methods of Obtaining Extracts from Hedera helix L. Leaves and Evaluation of the Total Saponins Content †

by
Rodica Tatia
1,
Lucia Moldovan
1,
Isabela Tarcomnicu
2,
Anca Daniela Raiciu
3,
Adina Gavrila
4,
Ioan Calinescu
4 and
Christina Zalaru
5,*
1
Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296 Splaiul Independenţei, 060031 Bucharest, Romania
2
Cytogenomic Medical Laboratory, 35 Floreasca Street, 014462 Bucharest, Romania
3
Hofigal Export-Import SRL, 2 Greenhouse Entrance Street, 042124 Bucharest, Romania
4
Department of Bioresources and Polymer Science, Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania
5
Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 90-92 Panduri Street, 050663 Bucharest, Romania
*
Author to whom correspondence should be addressed.
†
Presented at the 17th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 27–29 October 2021.
Chem. Proc. 2022, 7(1), 56; https://doi.org/10.3390/chemproc2022007056
Published: 24 March 2022
(This article belongs to the Proceedings of The 17th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM)
Versions Notes

Abstract

:
Hedera helix L. is known for its therapeutic properties, such as analgesic, anti-inflammatory, expectorant activity. It is currently known that the characteristic therapeutic effects of ivy extracts are induced by phytocompunds, such as: saponins (hederagenin, α and β-hederin, hederacoside B and C), phytosterols (sitosterol, stigmasterol, campesterol), flavonoids, falcarinol, falcarinone, scopoline, chlorogenic acid, caffeic acid, phytoestrogens [1]. The purpose of our study was to evaluate the total saponin content of Hedera helix L. leaves extracts obtained by both conventional, and unconventional methods. The commercial fresh leaves of Hedera helix L. were purchased from Hofigal SA, Romania. The following reagents used for testing were α-hederin, hederagenin, and hederacoside C at purity ≥98% (HPLC), DMSO were purchased from Sigma Aldrich. The chemical composition of the obtained extracts was analyzed by HPLC-MS/MS, and the total saponin content was evaluated [2,3]. Our study indicated an optimal method for obtaining Hedera helix L. leaves extract with an enriched saponin content.

Author Contributions

Conceptualization, C.Z. and I.C.; methodology, C.Z. and R.T.; validation, L.M., I.C. and C.Z.; resources, A.D.R. provided the plant material and made the qualitative phytochemical screening of the leaves; C.Z. obtained the extract by conventional methods; A.G. and I.C. obtained the extract by unconventional methods; R.T. determined the total saponin content; I.T. analyzed the samples tested by HPLC-MS/MS targeting quantification and interpreted the data; writing—original draft preparation, C.Z. and R.T.; writing supervision, L.M. and I.C.; funding acquisition, C.Z., R.T. and L.M. All authors have read and agreed to the published version of the manuscript.

Funding

This research was financed by the Project 381PED/2020.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available on request from the corresponding author. The data are not public available, because these will be published in an article.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Lutsenko, Y.; Bylka, W.; Matławska, I.; Darmohray, R. Hedera helix as a medicinal plant. Herba Pol. 2010, 56, 83–95. [Google Scholar]
  2. Hiai, S.; Oura, H.; Hakajima, T. Color reaction of some sapogenins and saponins with vanillin and sulfuric acid. Planta Med. 1976, 29, 116–122. [Google Scholar] [CrossRef] [PubMed]
  3. Wang, Y.; Yang, L.; Zhihui, L.; Jun, Y. The quality control of the effective fraction from Dioscoreaspongiosa. Asian J. Trad. Med. 2007, 2, 12–18. [Google Scholar]
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MDPI and ACS Style

Tatia, R.; Moldovan, L.; Tarcomnicu, I.; Raiciu, A.D.; Gavrila, A.; Calinescu, I.; Zalaru, C. Methods of Obtaining Extracts from Hedera helix L. Leaves and Evaluation of the Total Saponins Content. Chem. Proc. 2022, 7, 56. https://doi.org/10.3390/chemproc2022007056

AMA Style

Tatia R, Moldovan L, Tarcomnicu I, Raiciu AD, Gavrila A, Calinescu I, Zalaru C. Methods of Obtaining Extracts from Hedera helix L. Leaves and Evaluation of the Total Saponins Content. Chemistry Proceedings. 2022; 7(1):56. https://doi.org/10.3390/chemproc2022007056

Chicago/Turabian Style

Tatia, Rodica, Lucia Moldovan, Isabela Tarcomnicu, Anca Daniela Raiciu, Adina Gavrila, Ioan Calinescu, and Christina Zalaru. 2022. "Methods of Obtaining Extracts from Hedera helix L. Leaves and Evaluation of the Total Saponins Content" Chemistry Proceedings 7, no. 1: 56. https://doi.org/10.3390/chemproc2022007056

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