Bacillus subtilis Provides Long-Term Protection in a Murine Model of Allergic Lung Disease by Influencing Bacterial Composition
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Allergic Lung Disease Model
2.3. ELISA
2.4. DNA Extraction and 16S rRNA Amplicon Sequencing
2.5. Statistics
3. Results
3.1. Bacillus Subtilis Protection from House-Dust Mite-Induced Eosinophilia Wanes after 3 Weeks
3.2. CCL24 Production Is Suppressed by Bacillus subtilis Treatment for 3 Weeks
3.3. B. subtilis Treatment Does Not Influence Immunoglobulin Production
3.4. Bacillus Fecal Composition Is Similar across Mice Regardless of Treatment with B. subtilis Spores
3.5. Bacterial Composition Clustering Correlates with Protection from Eosinophilia
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Monroy Del Toro, R.; Incrocci, R.; Negris, O.; McGrath, S.; Swartzendruber, J.A. Bacillus subtilis Provides Long-Term Protection in a Murine Model of Allergic Lung Disease by Influencing Bacterial Composition. Allergies 2023, 3, 1-10. https://doi.org/10.3390/allergies3010001
Monroy Del Toro R, Incrocci R, Negris O, McGrath S, Swartzendruber JA. Bacillus subtilis Provides Long-Term Protection in a Murine Model of Allergic Lung Disease by Influencing Bacterial Composition. Allergies. 2023; 3(1):1-10. https://doi.org/10.3390/allergies3010001
Chicago/Turabian StyleMonroy Del Toro, Rosalinda, Ryan Incrocci, Olivia Negris, Shaina McGrath, and Julie A. Swartzendruber. 2023. "Bacillus subtilis Provides Long-Term Protection in a Murine Model of Allergic Lung Disease by Influencing Bacterial Composition" Allergies 3, no. 1: 1-10. https://doi.org/10.3390/allergies3010001