Root Physiological Changes and Transcription Analysis of Iris domestica in Response to Persistent Drought
Round 1
Reviewer 1 Report
In the manuscript titled, 'Root physiological changes and transcription analysis of Iris domestica in response to persistent drought', the authors describe their work to characterize the transcriptional response of Iris domestica, a common garden plant with reported medicinal qualities, to drought stress. The authors report transcriptional changes at three key time points that were determined based on observed physiological changes in the plant.
I find the study very straightforward and logically organized. I will recommend this study for publication; however, there are a few things I would like fixed in the final manuscript.
First, in Fig 2c and 2d the y-axis labels should be consistent with journal preferences (unit A/unit B or unit A * unit B-1).
Secondly, please increase the font size of all text in the figures to make them more readable. Also, consider changing from yellow to a color that is easier to see on a white background if possible.
Finally, please include a pdf or text copy of all R scripts in the supplemental data. Complex figures like Figure 6 are difficult to reproduce and the inclusion of such scripts in the supplement will increase the citation rate of the paper and make the data easier to reproduce.
Author Response
Please see the attachment.
Author Response File: 
Author Response.docx
Reviewer 2 Report
The MS is devoted to the study of the effect of drought on the iris plants.
It is necessary to explain in the materials and methods how isoflavanoids and POD were determined. According to the description, they were determined in dry roots.
How were they analyzed?
In the captions to Figure 2, it is not clear for the wet or dry mass, the composition was recalculated
What was ambient temperature, humidity, light intensity during the experiment?
Figure 1 shows soil moisture, but at what depth of the pot, the earth dries out unevenly
How was soil moisture determined?
Tell me if you managed to detect dehydrins in transcriptome, if yes, add to the text.
In the discussion, it is necessary to add that PCR confirmed transcriptomic analysis.
In fact, the discussion is rather superficial, because. discuss only peroxidase, flavonoid biosynthesis and some transfactors, it is interesting to know how things are with primary metabolites, sugars, etc., as well as hormone biosynthesis.
Author Response
Please see the attachment.
Author Response File: Author Response.docx
Round 2
Reviewer 1 Report
The author seems to have addressed most of my concerns for the paper.
Reviewer 2 Report
After reviewing, the article was improved on all comments, a response was received
