Variations in Flower Color of Mutant Chrysanthemums
Round 1
Reviewer 1 Report
Comments and Suggestions for Authors
Tissue culture was used to obtain chrysanthemum mutants isolating desirable properties with pink, white and chimeric flowers in this study. It is an interesting study, and make it possible to obtain new varieties by selecting individuals with superior characteristics in ornamental plants. I have several suggestions that authors may consider to improve the manuscript further.
1. As showing in the Abstract, “Observations of the mutants were collected during flowering time and 97 of the mutants were compared to the control group”, the number of mutants is 97. The number is described in the abstract, which is not found in Material and Methods or Results. Please add to this content where appropriate.
2. In Results, cluster analysis in the first, the result description in the second, which is not very reasonable. It is better to change the order of the two above.
3. The authors measured ten indexes, such as: plant height, plant diameter, number of leaves, leaf length, leaf width, number of flowers per plant, flower width, number of flower colors each plant, number of ray florets and stem weight. But in results, there are only plant heights (Figure 8) and flower numbers (Figure 9), and changing ratios of the population according to the control plants (Figure 7). Where are the other indexes? It would be better if something in this regard could be added.
4. The discussion section needs to be revised.
5. Line 196-198: While all characteristics have increased, the plant height, stem length, number of flowers and stem weight have decreased according to the control group (Figure 7). Where is stem length? There is no stem length in Figure 7.
Comments on the Quality of English LanguageThe quality of the English language in this article is very good.
Author Response
To the Editor,
I sincerely appreciate all of your time and hard work in contributing to this paper. I adjusted everything you recommended.
Best regards.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsAbstract
The authors did not focus on the most important results, they presented the most obtained results in detail. Please provide more specific results in the Abstract section.
Introduction
Line 60 – 66 the Introduction lacks a hypothesis and objectives. In this format, it is not clear what the exact goal of the research is, therefore the significance of the research is not highlighted.
Material and methods
Please give more information about radiation conditions
Line 71 Please explain the abbreviation M1V4
Line 76 – 84 Please present this part more clearly because it is so difficult to follow, and give more information that is more clearly explained.
Line 97 You wrote that the relative humidity was 55-60%. Where did you measure these values? In the storage room, or inside the vessels. In which vessels you kept the nodes culture? Inside the vessels, the relative humidity is higher than 55-60%, most often these are values of 90-95%. Please explain this and give more information about culture conditions.
Line 107 Where did you measure these values of relative humidity? Please explain this and give more information about those conditions.
Line 127-131 Whether the experiment had repetitions? If yes, please mention that information in the text. If not, the experiment without repetition does not give precise results.
Line 128-131 Please give the results of the parameters you mention in this sentence. You can add it in the paper, or as supplementary results.
Results
Line 149-151 Please, give the results of the parameters you mention in this sentence. You can add it in the paper, or as supplementary results.
Line 171 Where were presented this result? You wrote Table 2, but I don't see this value in Table 2.
Line 173 Where was this result presented?
Line 208 – 214 This sounds like a conclusion. Please rewrite or move it to the Conclusion
Discussion
Line 223-225 This sentence belongs to the Introduction
Conclusion
Line 260-268 This part belongs to the introduction. Please move it, or delete it from the text.
Author Response
Dear Editor,
Thank you very much for all your efforts and time for your valuable contributions to the article. I made all the changes you suggested. I would like to explain:
Line 127-131 Whether the experiment had repetitions? If yes, please mention that information in the text. If not, the experiment without repetition does not give precise results.
In our study, since each mutant was a single plant and they were not similar to each other, we could not have repetitions during the measurements.
Results
Line 171 Where were presented this result? You wrote Table 2, but I don't see this value in Table 2.
These values take place in the cumulative column as yellow highlighted as shown below:
We would like to use % values in the text.
Table 2. Eigenvalue, variation percentage and cumulative variation percentage values obtained as a result of principal component analysis in observed mutant genotypes
|
|
Eigenvalue |
Proportion |
Cumulative |
|
PC 1 |
2.6279 |
0.263 |
0.263 |
|
PC 2 |
1.4048 |
0.140 |
0.403 |
|
PC 3 |
1.2211 |
0.122 |
0.525 |
|
PC 4 |
1.1082 |
0.111 |
0.636 |
|
PC 5 |
0.9547 |
0.095 |
0.732 |
|
PC 6 |
0.7058 |
0.071 |
0.802 |
|
PC 7 |
0.6974 |
0.070 |
0.872 |
|
PC 8 |
0.6162 |
0.062 |
0.934 |
|
PC 9 |
0.3508 |
0.035 |
0.969 |
|
PC 10 |
0.3132 |
0.031 |
1 |
Line 173 Where was this result presented?
I table 2, but we have used this number as % value in the text. If you like, I can write the same value as it is.
Best regards
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
Comments and Suggestions for AuthorsIntroduction
Line 67 This sentence should have reference
Line 71-73 This sentence should have reference
Material and methods
Line 106-108 All those values should be presented in results (or like supplementary)
Line 124-126 Values of relative humidity in climate room were not important for the development of explants in the tissue culture. Please delete this sentence.
Results
The values in the text should be the same like in tables and figures. Adapt the whole results in the paper to it.
Line 157 Where were presented this results? Give the values in paper (or like supplementary)
Author Response
Dear editor,
I made the changes you requested in the article.
Thank you very much for your valuable contributions and time.
Best regards
Author Response File: Author Response.pdf
