Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Isolates Used
2.2. Cultivation of Fungi and Supernatant Harvesting
2.3. Xylanase Activity Measurements
2.4. Qualitative Plate-Based Assays for Ligninolytic Enzyme Activity
Polyphenol-Oxidase/Peroxidase-like Activity
2.5. Exo-Glycoside Hydrolase and Feruloyl Esterase Activity Measurements
2.6. Electrophoresis and Zymography
2.7. Statistics
3. Results and Discussion
3.1. Xylanase Induction by WB during LSF, and the Qualitative Assessment of Lignin-Degrading Activities
3.2. Exo-Acting Glycoside Hydrolase and Feruloyl Esterase Induction by WB during LSF
3.3. Effect of Temperature and pH on Xylanase Activity in the Fungal Secretomes
3.4. Electrophoretic Separation of Proteins in the Crude Secretomes and Zymogram Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dowd, B.; Tuohy, M.G. Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes. Fermentation 2023, 9, 780. https://doi.org/10.3390/fermentation9090780
Dowd B, Tuohy MG. Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes. Fermentation. 2023; 9(9):780. https://doi.org/10.3390/fermentation9090780
Chicago/Turabian StyleDowd, Bronwyn, and Maria G. Tuohy. 2023. "Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes" Fermentation 9, no. 9: 780. https://doi.org/10.3390/fermentation9090780