Survey on Yeast Assimilable Nitrogen Status of Musts from Native and International Grape Varieties: Effect of Variety and Climate
Round 1
Reviewer 1 Report
The manuscript entitled, “Survey on yeast assimilable nitrogen status of musts from native and international grape varieties: Effect of variety and climate” was committed to evaluate the Yeast assimilable nitrogen (YAN), besides the oenological parameters in grape musts of sixteen native and international varieties of Vitis vinifera cultivated in six regions of Northern Greece. The results showed that the content of the international grapes cultivated in Greece fell within the range noted by other researchers. About 36% of the Greek samples were below the deficiency threshold of 140 mg L-1, thus posing a danger of problems with the nutrition of yeasts in the course of fermentation unless it occurs an addition of a nitrogen source. This study revealed that not only grape variety but also cultivation region is a determinator of the concentration and composition of YAN. This work can be useful to understand how commercial varieties that are included in this study can behave in different climates in the contests of climate change. This study is complete and novel, but some obvious mistakes and details need to be further modified and improved. There are a few comments that need to be addressed prior to the publication.
1. Tables 2 and 3 should be in the same format. In other words, the first part in Table 2 is the sample name, and the first part is YAN, FAN, ammoniacal nitrogen and oenological parameters. In addition, the formatting of the comments in Table 2 is an obvious error, including indentation and font size.
2. The meaning of the letters in Table 2 should be further explained, and the way they are now labeled is difficult to understand.
3. The information contained in Figure 2 is already covered by Tables 2 and 3, and I think it should be placed in the supporting information.
4. The “p < 0.05”in the manuscript should be revised to “p < 0.05”.
5. The description of climate data is not accurate, so low rainfall and high rainfall should be converted into intuitive data. As mentioned below, >90 mm or >350 mm.
6. Figures 3 and 5 need to provide a higher resolution graph.
7. There are many problems in the format of references, which need to be checked carefully. Includes missing page numbers and DOIs, as well as uppercase or lowercase words. Such as No. 24, 28-29, 31, etc.
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The manuscript should be revised critically before acceptance.
Comments for author File: Comments.pdf
English language should be improved.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
The manuscript has been revised and accepted.
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