Exploiting Cheese Whey for Efficient Selection of Polyhydroxyalkanoates-Storing Bacteria

Round 1

Reviewer 1 Report

 

1. Title: The title of the manuscript accurately reflects the content of the paper.
2. Abstract and Key words:

The abstract lacks information about the tea classification method. Key words are adequate to the content of the manuscript.

3. Introduction: informative.
4. Objectives and hypotheses: The aim of the study was clearly stated. The hypotheses was accurately connected with state-of-knowledge in the discussion section.
5. Methods: adequate to the aims of the study.
6. Results and Discussion: Table 2 should be corrected. The standard deviation should be in the same line with the results. The legend (Table 2)  should not be in the middle of the table. Figure captions should be standardized either in italics or not. The caption under Figure 1 is duplicated. In this arrangement, Fig. No. 3 is missing. Blank is missing in Figure 4.
7. Literature cited: relevant.
8. Additional comments:

·       Lines 124-127 should not be in italics. Text should be aligned.

·       Lines 129-134 should not be in italics. Text should be aligned.

·       There should be a pause before line 228.

 

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Author Response

 

 

1. Title: The title of the manuscript accurately reflects the content of the paper.
2. Abstract and Key words:

The abstract lacks information about the tea classification method. Key words are adequate to the content of the manuscript.

1. Introduction: informative.
2. Objectives and hypotheses: The aim of the study was clearly stated. The hypotheses was accurately connected with state-of-knowledge in the discussion section.
3. Methods: adequate to the aims of the study.
4. Results and Discussion: Table 2 should be corrected. The standard deviation should be in the same line with the results. The legend (Table 2) should not be in the middle of the table. Figure captions should be standardized either in italics or not. The caption under Figure 1 is duplicated. In this arrangement, Fig. No. 3 is missing. Blank is missing in Figure 4. Checked.
5. Literature cited: relevant.
6. Additional comments:

• Lines 124-127 should not be in italics.Text should be aligned. Checked
• Lines 129-134 should not be in italics.Text should be aligned. Checked
• There should be a pause before line 228. Checked

Reviewer 2 Report

This article described the isolation of PHA storing MMC from cheese whey using a SBR-based domestication method. PHA production is a hot topic thus this article would attract interests from readers.

1.     The text in section 2.3 and 2.4 are in italic.

2.     The analytical methods in section 2.3 should be briefly described instead of simply citing references.

3.     Fig 1 showed the SBR cycle on day 22, which should be described in the figure legend. The cycle number should also be explained.

4.     The font in the legend of Fig 3, which was wrongly labelled as Fig 1, should be modified.

5.     What does X/S stand for?

6.     Figure 4, 500bp. Why there were bands in lane B and C not analyzed? The representative species should be labelled with the bands on the figure.

7.     How the results shown in line 405-410 obtained? How were the fitted curve shown in Fig 5 obtained? How were the kinetics parameters determined?

 

8.     Did the authors try to isolate the PHA producing strains based on the DGGE result? It would be interesting to understand the diversity of the MMC and the contribution to PHA production, which could be discussed in the article.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf