In Situ Forming Bioartificial Hydrogels with ROS Scavenging Capability Induced by Gallic Acid Release with Potential in Chronic Skin Wound Treatment
Abstract
:1. Introduction
2. Results and Discussion
2.1. Poly(Ether Urethane) Chemical Characterization
2.2. Bioartificial Hydrogel Properties in Physiological-Mimicking Conditions
2.2.1. Hydrogel Thermo-Responsiveness
2.2.2. Hydrogel Responsiveness to Physiological-like Fluids
2.3. In Vitro GA Release Test and Assessment of Its Scavenging Properties
2.4. In Vitro Evaluation of System Cytocompatibility
2.5. GA Protective Effects under Induced Oxidative Stress
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Poly(Ether Urethane) Synthesis and Exposure of Secondary Amino Groups
4.3. Poly(Ether Urethane) Chemical Characterization
4.4. Bioartificial Hydrogel Preparation
4.5. Qualitative Evaluation of the Thermo-Sensitivity of Macromolecular Hydrogels
4.6. Rheological Characterization of Macromolecular Hydrogels
4.7. Bioartificial Hydrogel Responsiveness to Physiological-Like Fluids
4.8. Preparation of Gallic Acid-Loaded Hydrogels
4.9. Gallic Acid Release Study
4.10. In Vitro Assessment of ROS Scavenging Effects
4.11. Preliminary Assessment of Hydrogel Cytocompatibility
4.11.1. Cytocompatibility of Hydrogel Extracts
4.11.2. Cytocompatibility of Gallic Acid
4.12. Cell Protective Effect Exerted by Released GA
4.12.1. Induction of an In Vitro ROS Microenvironment
4.12.2. Measurement of Intracellular ROS under GA Scavenging Activity
4.13. Immunofluorescence Staining to Study Morphological Changes in Fibroblast Cytoskeleton
4.14. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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HA (% w/v) | D-DHP407 (% w/v) | Solvent | |
---|---|---|---|
HA/D-DHP407_5%_ddH2O | 5.0% | 5.0% | ddH2O |
HA/D-DHP407_6%_ddH2O | 6.0% | 6.0% | ddH2O |
HA/D-DHP407_7.5%_ddH2O | 7.5% | 7.5% | ddH2O |
HA/D-DHP407_5%_DPBS | 5.0% | 5.0% | DPBS |
HA/D-DHP407_6%_DPBS | 6.0% | 6.0% | DPBS |
HA/D-DHP407_7.5%_DPBS | 7.5% | 7.5% | DPBS |
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Laurano, R.; Torchio, A.; Ciardelli, G.; Boffito, M. In Situ Forming Bioartificial Hydrogels with ROS Scavenging Capability Induced by Gallic Acid Release with Potential in Chronic Skin Wound Treatment. Gels 2023, 9, 731. https://doi.org/10.3390/gels9090731
Laurano R, Torchio A, Ciardelli G, Boffito M. In Situ Forming Bioartificial Hydrogels with ROS Scavenging Capability Induced by Gallic Acid Release with Potential in Chronic Skin Wound Treatment. Gels. 2023; 9(9):731. https://doi.org/10.3390/gels9090731
Chicago/Turabian StyleLaurano, Rossella, Alessandro Torchio, Gianluca Ciardelli, and Monica Boffito. 2023. "In Situ Forming Bioartificial Hydrogels with ROS Scavenging Capability Induced by Gallic Acid Release with Potential in Chronic Skin Wound Treatment" Gels 9, no. 9: 731. https://doi.org/10.3390/gels9090731