Carboxymethyl Chitosan Microgels for Sustained Delivery of Vancomycin and Long-Lasting Antibacterial Effects
Abstract
:1. Introduction
2. Results and Discussion
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Synthesis and Characterization of CMCh Microgels
4.3. Degradation Profile of CMCh Microgels
4.4. In Vitro Cell Compatibility Studies of CMCh Microgels
4.5. Drug Delivery Abilities of CMCh Microgels
4.6. Antibacterial Activities of Van@CMCh Microgels
4.6.1. Broth Micro-Titer Dilution Assay
4.6.2. Zone of Inhibition Method
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kinetic Model | Parameters | Van@CMCh Microgels |
---|---|---|
Zero order | k0 | 25.582 |
R2 | 0.9652 | |
First order | k1 | 0.327 |
R2 | 0.9264 | |
Higuchi | kH | 30.667 |
R2 | 0.8257 | |
Korsmeyer–Peppas | kKP | 22.437 |
n | 1.245 | |
R2 | 0.9763 |
Microorganisms | E. coli | S. aureus | ||
---|---|---|---|---|
Sample | MIC (µg/mL) | MBC (µg/mL) | MIC (µg/mL) | MBC (µg/mL) |
CMCh | - | - | - | - |
CMCh microgels | - | - | - | - |
Van@CMCh microgels | 64 | - | 8 | 8 |
Vancomycin * | 15 | 250 | 0.2 | 0.2 |
Inhibition Zone Diameter (mm) | 24 h | 48 h | 96 h |
---|---|---|---|
E. coli | - | - | 11.5 ± 1 |
S. aureus | 11.6 ± 1.5 | 12.5 ± 1 | 14.5 ± 1.5 |
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Sahiner, M.; Yilmaz, A.S.; Ayyala, R.S.; Sahiner, N. Carboxymethyl Chitosan Microgels for Sustained Delivery of Vancomycin and Long-Lasting Antibacterial Effects. Gels 2023, 9, 708. https://doi.org/10.3390/gels9090708
Sahiner M, Yilmaz AS, Ayyala RS, Sahiner N. Carboxymethyl Chitosan Microgels for Sustained Delivery of Vancomycin and Long-Lasting Antibacterial Effects. Gels. 2023; 9(9):708. https://doi.org/10.3390/gels9090708
Chicago/Turabian StyleSahiner, Mehtap, Aynur S. Yilmaz, Ramesh S. Ayyala, and Nurettin Sahiner. 2023. "Carboxymethyl Chitosan Microgels for Sustained Delivery of Vancomycin and Long-Lasting Antibacterial Effects" Gels 9, no. 9: 708. https://doi.org/10.3390/gels9090708