Response Surface Methodology for Optimization of Hydrogel-Forming Microneedles as Rapid and Efficient Transdermal Microsampling Tools
Abstract
:1. Introduction
2. Results and Discussion
2.1. Experimental Design
2.2. Hydrogel Films
2.3. Fabrication and Characterization of Hydrogel-Forming MNs
3. Conclusions
4. Materials and Methods
4.1. Materials and Chemicals
4.2. Experimental Design
4.3. Hydrogel Films
4.3.1. Preparation Procedure
4.3.2. Characterization
4.4. Fabrication Method of Hydrogel-Forming MNs
4.5. Characterization of Hydrogel-Forming MNs
4.5.1. Morphology
4.5.2. Swelling Test
4.5.3. Mechanical Strength Test
4.5.4. Skin Insertion Test
4.5.5. In Vitro ISF Extraction and Recovery Test
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Model | Variables and Level | F-Value | p-Value | R2 | Adjusted R2 | Predicted R2 | Adeq Precision | PRESS |
---|---|---|---|---|---|---|---|---|
BBD | 2–8% w/w Hyaluronic acid, 1–2% w/w GantrezTM S-97, and 0.5–1.5% w/w Pectin | 120.34 | <0.0001 | 0.9722 | 0.9641 | 0.9505 | 32.71 | 2.35 × 105 |
CCD | 258.73 | <0.0001 | 0.9894 | 0.9856 | 0.9784 | 49.46 | 8.81 × 104 | |
Optimal discrete | 341.94 | <0.0001 | 0.9923 | 0.9894 | 0.9831 | 59.12 | 6.83 × 104 |
Characteristics | Value |
---|---|
Shape | Pyramidal |
Dimension (height × base width) | 525.4 ± 3.8 × 157.4 ± 2.0 µm |
Swelling ability | 1508.2 ± 66.2% |
Collection volume | 124.6 ± 7.4 µL |
Recovery | 71.8 ± 3.2% to 78.3 ± 2.6% |
Skin insertion | Up to approx. 400 µm |
Mechanical strength | Withstands pressure from a finger |
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Leanpolchareanchai, J.; Nuchtavorn, N. Response Surface Methodology for Optimization of Hydrogel-Forming Microneedles as Rapid and Efficient Transdermal Microsampling Tools. Gels 2023, 9, 306. https://doi.org/10.3390/gels9040306
Leanpolchareanchai J, Nuchtavorn N. Response Surface Methodology for Optimization of Hydrogel-Forming Microneedles as Rapid and Efficient Transdermal Microsampling Tools. Gels. 2023; 9(4):306. https://doi.org/10.3390/gels9040306
Chicago/Turabian StyleLeanpolchareanchai, Jiraporn, and Nantana Nuchtavorn. 2023. "Response Surface Methodology for Optimization of Hydrogel-Forming Microneedles as Rapid and Efficient Transdermal Microsampling Tools" Gels 9, no. 4: 306. https://doi.org/10.3390/gels9040306