Paracoccidioides brasiliensis Induces α3 Integrin Lysosomal Degradation in Lung Epithelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Epithelial Cells Culture
2.2. Culture and Preparation of P. brasiliensis Yeasts for Interaction Assays with Epithelial Cells
2.3. Interaction of Epithelial Cells with P. brasiliensis Yeasts
2.4. Small Interfering RNA (siRNA)
2.5. Obtaining Endosome-Enriched Fractions
2.6. Co-Immunoprecipitation
2.7. Western Blot
2.8. Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR)
2.9. Immunofluorescence
2.10. Cell Viability
3. Results
3.1. Analysis of α3 Integrin Levels in A549 Cells Infected with P. brasiliensis Yeasts
3.2. Subcellular Localization of α3 Integrin in A549 Epithelial Cells during Infection with P. brasiliensis
3.3. Presence of α3 Integrin in Late Endosome-Enriched Fractions of Epithelial Cells during P. brasiliensis Infection
3.4. α3 Integrin Levels in CHMP3-Silenced A549 Epithelial Cells Infected with P. brasiliensis
3.5. Analysis of α3 Integrin Levels in A549 Epithelial Cells during Infection with P. brasiliensis in the Presence of Bafilomycin A1
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Almeida, B.R.; Barros, B.C.S.C.; Barros, D.T.L.; Orikaza, C.M.; Suzuki, E. Paracoccidioides brasiliensis Induces α3 Integrin Lysosomal Degradation in Lung Epithelial Cells. J. Fungi 2023, 9, 912. https://doi.org/10.3390/jof9090912
Almeida BR, Barros BCSC, Barros DTL, Orikaza CM, Suzuki E. Paracoccidioides brasiliensis Induces α3 Integrin Lysosomal Degradation in Lung Epithelial Cells. Journal of Fungi. 2023; 9(9):912. https://doi.org/10.3390/jof9090912
Chicago/Turabian StyleAlmeida, Bruna Rocha, Bianca Carla Silva Campitelli Barros, Debora Tereza Lucas Barros, Cristina Mary Orikaza, and Erika Suzuki. 2023. "Paracoccidioides brasiliensis Induces α3 Integrin Lysosomal Degradation in Lung Epithelial Cells" Journal of Fungi 9, no. 9: 912. https://doi.org/10.3390/jof9090912