In Vitro Activity of Novel Lipopeptides against Triazole-Resistant Aspergillus fumigatus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolates
2.2. Antifungals
2.3. Peptide Synthesis
2.4. Preparation of Fungal Suspensions
2.5. MIC Determination
2.6. Time–Kill Assay
2.7. XTT Assay
2.8. Optical Microscopy
2.9. Checkerboard Assays
2.10. Statistical Analysis
3. Results
3.1. Time–Kill Assay
3.2. XTT Assay
3.3. Optical Microscopy
3.4. Checkerboard Assays
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strains | MIC (µg/mL) | ||
---|---|---|---|
C14-NleRR-NH2 | C14-WRR-NH2 | Isavuconazole | |
A. fumigatus SSI-4524 | 8 | 8 | 8 |
A. fumigatus SSI-5586 | 16 | 16 | 1 |
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Fioriti, S.; Cirioni, O.; Simonetti, O.; Franca, L.; Candelaresi, B.; Pallotta, F.; Neubauer, D.; Kamysz, E.; Kamysz, W.; Canovari, B.; et al. In Vitro Activity of Novel Lipopeptides against Triazole-Resistant Aspergillus fumigatus. J. Fungi 2022, 8, 872. https://doi.org/10.3390/jof8080872
Fioriti S, Cirioni O, Simonetti O, Franca L, Candelaresi B, Pallotta F, Neubauer D, Kamysz E, Kamysz W, Canovari B, et al. In Vitro Activity of Novel Lipopeptides against Triazole-Resistant Aspergillus fumigatus. Journal of Fungi. 2022; 8(8):872. https://doi.org/10.3390/jof8080872
Chicago/Turabian StyleFioriti, Simona, Oscar Cirioni, Oriana Simonetti, Lucia Franca, Bianca Candelaresi, Francesco Pallotta, Damian Neubauer, Elzbieta Kamysz, Wojciech Kamysz, Benedetta Canovari, and et al. 2022. "In Vitro Activity of Novel Lipopeptides against Triazole-Resistant Aspergillus fumigatus" Journal of Fungi 8, no. 8: 872. https://doi.org/10.3390/jof8080872