A Polyclonal SELEX Aptamer Library Allows Differentiation of Candida albicans, C. auris and C. parapsilosis Cells from Human Dermal Fibroblasts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cultivation of Cells
2.2. SELEX Procedure
2.3. Real-Time PCR
2.4. Analyses of Specific Aptamer Library
2.4.1. Fluorometric Assay
2.4.2. Microscopy
2.4.3. Flow-Cytometry
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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SELEX Round | tRNA and BSA [pmol] | Washing Steps with PBS | HDF Counter-Selection |
---|---|---|---|
1 | 600 | 1 | - |
2 | 900 | 2 | - |
3 | 1500 | 4 | - |
4 | 2100 | 6 (4–6 with 0.1% (w/v) BSA in PBS) | - |
5 | 2600 | 8 (5–8 with 0.1% (w/v) BSA in PBS) | - |
6 | 3000 | 8 (5–8 with 0.1% (w/v) BSA in PBS) | + |
7 | 3000 | 8 (5–8 with 0.1% (w/v) BSA in PBS) | + |
8 | 3000 | 8 (5–8 with 0.1% (w/v) BSA in PBS) | + |
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Kneißle, K.; Krämer, M.; Kissmann, A.-K.; Xing, H.; Müller, F.; Amann, V.; Noschka, R.; Gottschalk, K.-E.; Bozdogan, A.; Andersson, J.; et al. A Polyclonal SELEX Aptamer Library Allows Differentiation of Candida albicans, C. auris and C. parapsilosis Cells from Human Dermal Fibroblasts. J. Fungi 2022, 8, 856. https://doi.org/10.3390/jof8080856
Kneißle K, Krämer M, Kissmann A-K, Xing H, Müller F, Amann V, Noschka R, Gottschalk K-E, Bozdogan A, Andersson J, et al. A Polyclonal SELEX Aptamer Library Allows Differentiation of Candida albicans, C. auris and C. parapsilosis Cells from Human Dermal Fibroblasts. Journal of Fungi. 2022; 8(8):856. https://doi.org/10.3390/jof8080856
Chicago/Turabian StyleKneißle, Katharina, Markus Krämer, Ann-Kathrin Kissmann, Hu Xing, Franziska Müller, Valerie Amann, Reiner Noschka, Kay-Eberhard Gottschalk, Anil Bozdogan, Jakob Andersson, and et al. 2022. "A Polyclonal SELEX Aptamer Library Allows Differentiation of Candida albicans, C. auris and C. parapsilosis Cells from Human Dermal Fibroblasts" Journal of Fungi 8, no. 8: 856. https://doi.org/10.3390/jof8080856