Amplicon Sequencing Reveals Novel Fungal Species Responsible for a Controversial Tea Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. ITS-Based Amplicon Sequencing and Bioinformatic Analysis
2.3. Fungal Isolation
2.4. Virulence and Pathogenicity Tests
2.5. DNA Extraction, Amplification, and Sequencing
2.6. Phylogenetic Analysis
2.7. Morphological Assessments and Growth Rate
3. Results
3.1. ITS-Based Amplicon Sequencing Reveals the Candidate Etiology
3.2. Fungal Isolation Confirmed the ITS-Based Amplicon Sequencing Analysis
3.3. Virulence Tests on Detached Tea Leaves Reveal Didymella spp. as the Candidate Etiological Agents
3.4. Phylogenetic Analyses
3.5. Taxonomy
3.6. Fulfillment of Koch’s Postulates Confirms Both Didymella Species as the Etiological Agents
3.7. Virulence and Growth Rate of the Didymella Isolates
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fungal Species | Colonial Isolation Percentage (%) | |||
---|---|---|---|---|
JYC | SZX | WJT | NC | |
Didymella sp. | 20.0 | 21.1 | 20.0 | 0.0 |
Pestalotiopsis sp. | 0.0 | 21.1 | 55.0 | 5.6 |
Colletotrichum sp. | 20.0 | 21.1 | 0.0 | 22.2 |
Diaporthe sp. | 10.0 | 5.3 | 0.0 | 11.1 |
Alternaria sp. | 0.0 | 15.8 | 5.0 | 5.6 |
Setophoma sp. | 0.0 | 5.3 | 0.0 | 0.0 |
Arthrinium sp. | 0.0 | 0.0 | 10.0 | 0.0 |
Pseudopithomyces sp. | 0.0 | 0.0 | 5.0 | 0.0 |
Cercospora sp. | 10.0 | 0.0 | 0.0 | 0.0 |
Melanconiella sp. | 10.0 | 0.0 | 0.0 | 0.0 |
Phyllosticta sp. | 10.0 | 0.0 | 0.0 | 44.4 |
Unidentified | 20.0 | 10.5 | 5.0 | 11.1 |
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He, Y.; Li, Y.; Song, Y.; Hu, X.; Liang, J.; Shafik, K.; Ni, D.; Xu, W. Amplicon Sequencing Reveals Novel Fungal Species Responsible for a Controversial Tea Disease. J. Fungi 2022, 8, 782. https://doi.org/10.3390/jof8080782
He Y, Li Y, Song Y, Hu X, Liang J, Shafik K, Ni D, Xu W. Amplicon Sequencing Reveals Novel Fungal Species Responsible for a Controversial Tea Disease. Journal of Fungi. 2022; 8(8):782. https://doi.org/10.3390/jof8080782
Chicago/Turabian StyleHe, Yunqiang, Yan Li, Yulin Song, Xingming Hu, Jinbo Liang, Karim Shafik, Dejiang Ni, and Wenxing Xu. 2022. "Amplicon Sequencing Reveals Novel Fungal Species Responsible for a Controversial Tea Disease" Journal of Fungi 8, no. 8: 782. https://doi.org/10.3390/jof8080782