A Fungal Defensin Inhibiting Bacterial Cell-Wall Biosynthesis with Non-Hemolysis and Serum Stability
Abstract
:1. Introduction
2. Materials and Methods
2.1. Construction of Py4-Lipid II Complex
2.2. Molecular Dynamics (MD) Simulations
2.3. Identification of Chemically Synthesized Py4
2.4. Circular Dichroism (CD)
2.5. Antibacterial Assay
2.6. Membrane Permeability Assay
2.7. Scanning Electron Microscope (SEM)
2.8. Cytoplasmic Accumulation of UMP
2.9. Electrospray Ionization Mass Spectrometry (ESI-MS)
2.10. Hemolysis Assays and Stability
3. Results
3.1. Py4 Has Two Unique Point Mutation Sites and Exhibits a More Rigid Structure than Plectasin
3.2. Chemically Synthetic Py4 Adopt a CSαβ-Type Structure with Potent Antibacterial Activity
3.3. Py4 Inhibits Bacterial Cell-Wall Biosynthesis
3.4. Py4 Is a Non-Hemolytic Defensin with Serum Stability
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Strains | CL (µM) |
---|---|
Bacillus megaterium CGMCC 1.0459 | 0.67 |
Curtobacterium luteum | 0.03 |
Staphylococcus aureus CGMCC 1.89 | 1.22 |
Penicillin-sensitive Staphylococcus epidermidis P1111 | 4.03 |
Penicillin-resistant Staphylococcus epidermidis P1389 | 4.38 |
Methicillin-resistant Staphylococcus aureus P1374 | 2.69 |
Methicillin-resistant coagulase negative Staphylococci P1369 | 4.78 |
Methicillin-resistant Staphylococcus aureus P1386 | 2.51 |
Staphylococcus aureus J685 | 2.69 |
Staphylococcus aureus J698 | 2.69 |
Staphylococcus aureus J706 | 2.51 |
Staphylococcus warneri CGMCC 1.2824 | 1.06 |
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Qi, S.; Gao, B.; Zhu, S. A Fungal Defensin Inhibiting Bacterial Cell-Wall Biosynthesis with Non-Hemolysis and Serum Stability. J. Fungi 2022, 8, 174. https://doi.org/10.3390/jof8020174
Qi S, Gao B, Zhu S. A Fungal Defensin Inhibiting Bacterial Cell-Wall Biosynthesis with Non-Hemolysis and Serum Stability. Journal of Fungi. 2022; 8(2):174. https://doi.org/10.3390/jof8020174
Chicago/Turabian StyleQi, Sudong, Bin Gao, and Shunyi Zhu. 2022. "A Fungal Defensin Inhibiting Bacterial Cell-Wall Biosynthesis with Non-Hemolysis and Serum Stability" Journal of Fungi 8, no. 2: 174. https://doi.org/10.3390/jof8020174