Microcurrent Reverses Cigarette Smoke-Induced Angiogenesis Impairment in Human Keratinocytes In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Cell Lines
2.2. Preparation of Cigarette Smoke Extract (CSE)
2.3. Resazurin Conversion Assay
2.4. Sulforhodamine B (SRB) Staining Assay
2.5. Cell Viability Was Assessed by a Double-Staining Assay Using Calcium-AM/Hoechst 33342
2.6. MC Application
2.7. HaCaT Cells Stimulation and Tube Formation Assay
2.7.1. HaCaT Cells Stimulation and Supernatant Collection
2.7.2. Tube Formation Assay
2.8. Angiogenesis Array Assay
2.9. Identification of Candidate Molecules for Signaling Pathways Using the ChEA3 Tool and the KEGG Database
2.10. Western Blot
2.11. Statistics
3. Results
3.1. 3% CSE Did Not Significanty Affect HaCaT Cells Viability
3.2. 3% CSE Significantly Blunted the Angiogenic Potential by HaCaT Cells, and This Was Offset by 100 μA MC Stimulation
3.3. 100 μA MC Prevents the Detrimental Effects of 3% CSE on the Secretion of Pro-Angiogenic Factors by HaCaT Cells
3.4. Phosphatidyl-Inositol 3-Kinase/Serine-Threonine Kinase (PI3K-Akt), Mitogen-Activated Protein Kinases (MAPK), and Nuclear Factor Kappa-B (NFκB) Signaling Pathway May Be Involved in the Differential Expression of Angiogenic-Related Factors
3.5. MC Exposure Intensified PI3K-Akt and MAPK Signaling in CSE-Injured HaCaT Cells
3.6. Inhibition of PI3K-Akt Signaling Deprived MC of Its Therapeutic Effect on the Tube Formation Function of CSE-Injured HaCaT Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
CS | cigarette smoking |
CSE | cigarette smoke extract |
ECL | enhanced chemiluminescence |
ES | electrical stimulation |
FBS | fetal bovine serum |
HaCaT | human keratinocyte cell line |
MAPK | mitogen-activated protein kinases |
MC | microcurrent |
MMP-1 | matrix metalloproteinase-1 |
NFκB | nuclear factor kappa-B |
PI3K-Akt | phosphatidyl-inositol 3-kinase/serine-threonine kinase |
SRB | sulforhodamine B |
TEP | transepithelial potential difference |
TGF-β | transforming growth factor beta |
TIMP-2 | tissue inhibitor of metalloproteinases 2 |
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Lu, C.; Prahm, C.; Chen, Y.; Ehnert, S.; Rinderknecht, H.; McCaig, C.D.; Nussler, A.K.; Kolbenschlag, J. Microcurrent Reverses Cigarette Smoke-Induced Angiogenesis Impairment in Human Keratinocytes In Vitro. Bioengineering 2022, 9, 445. https://doi.org/10.3390/bioengineering9090445
Lu C, Prahm C, Chen Y, Ehnert S, Rinderknecht H, McCaig CD, Nussler AK, Kolbenschlag J. Microcurrent Reverses Cigarette Smoke-Induced Angiogenesis Impairment in Human Keratinocytes In Vitro. Bioengineering. 2022; 9(9):445. https://doi.org/10.3390/bioengineering9090445
Chicago/Turabian StyleLu, Chao, Cosima Prahm, Yangmengfan Chen, Sabrina Ehnert, Helen Rinderknecht, Colin D. McCaig, Andreas K. Nussler, and Jonas Kolbenschlag. 2022. "Microcurrent Reverses Cigarette Smoke-Induced Angiogenesis Impairment in Human Keratinocytes In Vitro" Bioengineering 9, no. 9: 445. https://doi.org/10.3390/bioengineering9090445