Transcriptome-Wide Analysis Revealed the Potential of the High-Affinity Potassium Transporter (HKT) Gene Family in Rice Salinity Tolerance via Ion Homeostasis
Abstract
:1. Introduction
2. Materials and Methods
2.1. HKT Genes Family Identification
2.2. Phylogenetic Tree and Digital Expression
2.3. Chromosomal Locations and Protein–Protein Interactions of OsHKT Genes
2.4. Gene Structure and Conserved Motif Analysis
2.5. Gene Ontology and Cis-Elements Analysis of HKT Family Genes
2.6. RNA Isolation and cDNA Synthesis
2.7. Expression Profiling of HKT Genes in Oryza sativa
2.8. Na+, K+, and Their Ratio in Tolerant (3Y9H) and Sensitive (JLY252) Cultivars
2.8.1. Experimental Method
2.8.2. Measurements of Na+, K+ Ratio
2.9. Statistical Analysis
3. Results
3.1. Phylogenetic Analysis of OsHKT
3.2. Gene Structure and Conserved Motif Analysis of OsHKT Genes
3.3. Gene Ontology of OsHKT Genes in Rice
3.4. Cis-Elements of OsHKT Genes in Rice
3.5. Protein–Protein Interactions of OsHKT
3.6. Tissue-Specific Expression Analysis of OsHKT Genes in Rice
3.7. Expression Analysis of OsHKT Genes in Rice Roots under Brassinosteroids Application
3.8. Expression Analysis of OsHKT Genes in Rice Root under Jasmonic Acid Application
3.9. Expression Analysis of OsHKT Genes in Rice Roots and Stems under Saline Conditions
3.10. Expression Analysis of OsHKT Genes in Salinity Resistant Cultivar Subjected to Seawater Stress
3.11. Na+, K+ Ratio in Tolerant Cultivar 3Y9H
3.12. Na+, K+ Ratio in the Sensitive Cultivar (JLY252)
4. Discussion
4.1. OsHKT Genes Are Distributed Widely in the Rice Genome
4.2. OsHKT Genes Control Plant Response to Salt Stress, Brassinosteroids, and JA Treatments
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Locus ID | CDS | Chr. Position | AA | MW | PI | SL |
---|---|---|---|---|---|---|---|
OsHKT1 | LOC_Os06g48,810 | 1593 | Chr6- 29,541,219-29,538,934 | 531 | 59,295.0781 | 9.82610035 | PM |
OsHKT3 | LOC_Os01g34,850 | 1530 | Chr1- 19,242,042-19,243,853 | 510 | 56,374.3789 | 9.11009979 | PM |
OsHKT4 | LOC_Os04g51,820 | 1659 | Chr4- 30,727,084-30,724,244 | 553 | 61,862.4492 | 8.86629963 | PM |
OsHKT6 | LOC_Os02g07,830 | 1596 | Chr2- 4,103,333-4,105,657 | 532 | 59,304.5312 | 9.78820038 | PM |
OsHKT7 | LOC_Os04g51,830 | 1503 | Chr4- 30,739,334-30,734,183 | 501 | 54,239.3008 | 8.87370014 | PM |
OsHKT8 | LOC_Os01g20,160 | 1665 | Chr1- 11,463,442-11,458,955 | 555 | 60,218.2812 | 8.68159962 | PM |
OsHKT9 | LOC_Os06g48,800 | 1530 | Chr6- 29,536,553-29,534,805 | 510 | 56,116.7188 | 8.54440022 | PM |
Reference Protein | Predicted Interactive Partner | Annotation and Putative Function |
---|---|---|
OsHKT1 | NHX2 | Putative Na+/H+ antiporter; Sodium/hydrogen exchanger. |
NHX1 | Sodium/hydrogen exchanger; Belongs to the monovalent cation: proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. | |
P5CS1 | Delta-1-pyrroline-5-carboxylate synthase Glutamate 5-kinase Gamma-glutamyl phosphate reductase; P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants. Involved in abiotic stress tolerance. | |
HAK1 | Potassium transporter 1; High-affinity potassium transporter. Additionally, transport rubidium, with the same affinity and cesium, with a lower affinity; which belongs to the HAK/KUP transporter (TC 2.A.72.3) family. | |
OsJ_04382 | Putative BTB and TAZ domain protein. | |
TPKC | Two pore potassium channel c; Inward-rectifying potassium channel; Belongs to the two-pore domain potassium channel (TC 1.A.1.7) family. | |
OsJ_30786 | Hsp20/alpha crystalline family protein expressed; Belongs to the small heat shock protein (HSP20) family. |
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Hussain, S.; Zhang, R.; Liu, S.; Li, R.; Zhou, Y.; Chen, Y.; Hou, H.; Dai, Q. Transcriptome-Wide Analysis Revealed the Potential of the High-Affinity Potassium Transporter (HKT) Gene Family in Rice Salinity Tolerance via Ion Homeostasis. Bioengineering 2022, 9, 410. https://doi.org/10.3390/bioengineering9090410
Hussain S, Zhang R, Liu S, Li R, Zhou Y, Chen Y, Hou H, Dai Q. Transcriptome-Wide Analysis Revealed the Potential of the High-Affinity Potassium Transporter (HKT) Gene Family in Rice Salinity Tolerance via Ion Homeostasis. Bioengineering. 2022; 9(9):410. https://doi.org/10.3390/bioengineering9090410
Chicago/Turabian StyleHussain, Shahid, Rui Zhang, Shuli Liu, Rongkai Li, Yicheng Zhou, Yinglong Chen, Hongyan Hou, and Qigen Dai. 2022. "Transcriptome-Wide Analysis Revealed the Potential of the High-Affinity Potassium Transporter (HKT) Gene Family in Rice Salinity Tolerance via Ion Homeostasis" Bioengineering 9, no. 9: 410. https://doi.org/10.3390/bioengineering9090410