Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. HPLC Condition
2.3. Validation of Pyrogallol
2.3.1. Calibration Standards and QC Sample
2.3.2. Accuracy and Precision
2.4. In Vitro Dermal Absorption
2.5. Analysis Preparation
3. Results
3.1. Linearity of Calibration Standards
3.2. Accuracy and Precision
3.3. In Vitro Dermal Absorption Experiment
3.4. Permeation Parameter
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Item | Substance | Ref. |
---|---|---|
IUPAC Name 1 | benzene-1,2,3-triol | [1] |
CAS NO. 2 | 87-66-1 | |
Empirical formula | C6H6O3 | |
Structural formula | | |
MW 3 | 126.11 g/mol | |
XLogP3 4 | 0.5 | |
Physical form | powder | |
Solubility | Very soluble in ethanol, diethylether | |
Solubility in water | 1 g dissolves in 1.7 mL water | |
Synonyms | 1,2,3-Trihydroxybenzene |
Item | Condition |
---|---|
Column | Luna 3u C18(2) 100A (150 × 3.00 mm) |
Mobile phase | DW (0.1% formic acid): ACN = 95:5 |
LC condition | Isocratic |
Wavelength | 200 nm |
Column oven temperature | 30 °C |
Autosampler temperature | 4 °C |
Flow rate | 0.35 mL/min |
Running time | 6.5 min |
Injection volume | 10 μL |
Retention time | 5.4 min |
Sample | Concentration (μg/mL) | Linearity (r2) | Intra 1-Day | Inter 2-Day | ||
---|---|---|---|---|---|---|
Accuracy 3 (%) | Precision 4 (%) | Accuracy (%) | Precision (%) | |||
SWAB 5 | 0.5 | 0.99987 | 103.8 | 4.9 | 103.9 | 4.0 |
1.5 | 100.2 | 1.5 | 103.5 | 2.1 | ||
7.5 | 99.9 | 1.3 | 100.9 | 1.1 | ||
15 | 101.6 | 0.5 | 102.2 | 1.1 | ||
SC 6 | 0.5 | 0.99953 | 106.1 | 1.6 | 105.4 | 1.7 |
1.5 | 102.1 | 3.3 | 103.9 | 2.0 | ||
7.5 | 101.0 | 1.1 | 100.7 | 0.8 | ||
15 | 102.4 | 1.8 | 103.4 | 1.1 | ||
SKIN 7 | 0.5 | 0.99948 | 103.9 | 2.4 | 104.9 | 3.3 |
1.5 | 101.4 | 3.2 | 102.3 | 2.5 | ||
7.5 | 99.2 | 1.0 | 101.2 | 0.8 | ||
15 | 101.0 | 2.0 | 102.3 | 1.1 | ||
RF 8 | 0.5 | 0.99939 | 105.3 | 6.4 | 110.1 | 9.5 |
1.5 | 107.5 | 1.5 | 108.2 | 5.3 | ||
7.5 | 97.6 | 3.4 | 98.0 | 3.5 | ||
15 | 92.1 | 9.0 | 100.4 | 6.2 |
Pyrogallol Content (%) 1 (Mean ± Standard Deviation) | |
---|---|
SWAB (30 min) | 48.4 ± 7.6 |
SWAB (24 h) | 3.9 ± 0.8 |
2 SC | 2.3 ± 0.7 |
3 SKIN | 10.7 ± 4.7 |
4 RF | 15.3 ± 1.6 |
Total dermal absorption | 26.0 ± 3.9 |
Total recovery | 80.5 ± 6.8 |
Permeation Parameter Js (Equilibrium Flux, μg/cm2/h) | |
---|---|
Pyrogallol | 1.27 ± 0.13 |
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Kim, Y.-J.; Kim, H.-Y.; Lee, J.-D.; Kim, H.-Y.; Im, J.-E.; Kim, K.-B. Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient. Toxics 2022, 10, 570. https://doi.org/10.3390/toxics10100570
Kim Y-J, Kim H-Y, Lee J-D, Kim H-Y, Im J-E, Kim K-B. Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient. Toxics. 2022; 10(10):570. https://doi.org/10.3390/toxics10100570
Chicago/Turabian StyleKim, Yu-Jin, Hyang-Yeon Kim, Jung-Dae Lee, Hong-Yoon Kim, Jueng-Eun Im, and Kyu-Bong Kim. 2022. "Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient" Toxics 10, no. 10: 570. https://doi.org/10.3390/toxics10100570