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Article

The Fading Wild Plant Food–Medicines in Upper Chitral, NW Pakistan

by
Muhammad Abdul Aziz
1,*,
Zahid Ullah
2,
Muhammad Adnan
3,
Renata Sõukand
4 and
Andrea Pieroni
1,5
1
University of Gastronomic Sciences, Piazza Vittorio Emanuele II 9, 12042 Pollenzo, Italy
2
Center for Plant Sciences and Biodiversity, University of Swat, Kanju 19201, Pakistan
3
Department of Botanical and Environmental Sciences, Kohat University of Science and Technology, Kohat 26000, Pakistan
4
Department of Environmental Sciences, Informatics and Statistics, Ca’ Foscari University of Venice, Via Torino 155, 30172 Venezia, Italy
5
Department of Medical Analysis, Tishk International University, Erbil 4401, Iraq
*
Author to whom correspondence should be addressed.
Foods 2021, 10(10), 2494; https://doi.org/10.3390/foods10102494
Submission received: 14 September 2021 / Revised: 11 October 2021 / Accepted: 15 October 2021 / Published: 18 October 2021
(This article belongs to the Special Issue Plant Food-Medicines: Perceptions, Traditional Uses and Health Benefits of Food Botanicals, Mushrooms, and Herbal Teas)
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Abstract

:
The subject of food–medicines (foods ingested in order to obtain a therapeutic activity or to prevent diseases) is garnering increasing attention from both ethnobiologists and ethnopharmacologists as diet-related chronic diseases are one of the major problems resulting in a large proportion of deaths globally, which calls for interest from the scientific community to make sensible decisions in the field of food and medicine. In this regard, the current study is an important attempt at providing baseline data for developing healthy and curative food ingredients. This study aimed at recording the culinary and medicinal uses of wild food plants (WFPs) in the remote Mastuj Valley, located at the extreme north of Chitral District, Pakistan. An ethnobotanical survey was completed via 30 in-depth semi-structured interviews with local knowledge holders to record the food and medicinal uses of WFPs in the study area. A total of 43 WFPs were recorded, most of which were used as cooked vegetables and raw snacks. Leaves were the most frequently used plant part. A remarkable proportion (81%) of use reports for the recorded wild plant taxa were quoted as food–medicines or medicinal foods, while very few were reported as either food or medicines, without any relationship between uses in these two domains. Previous ethnomedicinal studies from nearby regions have shown that most of the recorded wild plants have been used as medicines, thus supporting the findings of the current study. A literature survey revealed that many of the reported medicinal uses (33%) for the quoted WFPs were not verifiable on PubMed as they have not been studied for their respective medicinal actions. We observed that most of the plants quoted here have disappeared from the traditional food and medicinal system, which may be attributed to the invasion of the food market and the prevalence of allopathic medicine. However, knowledge of these wild plants is still alive in memory, and women are the main holders of cultural knowledge as they use it to manage the cooking and processing of WFPs. Therefore, in this context, we strongly recommend the preservation of local biocultural heritage, promoted through future development and educational programs, which could represent a timely response to the loss of cultural and traditional knowledge.

1. Introduction

The famous saying “Let food be thy medicine and medicine be thy food” by Hippocrates is finding an important place in today’s world full of diet-related chronic diseases. The Greeks were not the first humans to focus on the medicinal value of food as there had been other traditional medical systems, including Ayurvedic and Traditional Chinese Medicine, that promoted the idea of food being medicine at the same time [1]. Diet has remained an important factor in preventing certain diseases and it always played a pivotal role in the early history of many Western biomedicines [2]. A perfect diet is a powerful tool in maintaining a healthy life [3], but it is a misfortune that the prevalence of an unhealthy diet has given rise to serious chronic diseases in people of the so-called modern world [4]. We are living in “a time of crisis” where one fifth of all deaths are attributed to a suboptimal diet [5]. In this widespread situation, individual interactions with the healthcare system are an important opportunity to provide a set of evidence-based food and nutrition interventions [6]. Research has shown that these interventions introduced into the healthcare system might be linked to improved health outcomes and reduced healthcare costs and usage [7,8,9]. It is believed that the close integration of nutrition interventions and food could provide a solid foundation for the initiative to include medically tailored meals, integrating nutritional strategies into healthcare systems, which is generally referred to as “food is medicine” [6].
Wild food plants (WFPs) have played an important role in feeding humans for millennia, and they are considered an alternative source of healthy and nutritious food [10,11]. The literature indicates that nearly 7000 wild species are recognized as edible [12] and many of them have proven medicinal benefits, producing positive health outcomes, thus creating a solid foundation for generating novel functional foods [13]. Shikov et al. [13], referring to Gammerman and Grom (1976), stated that WFPs have been selected not only for their good taste or smell, but also for their important pharmacological potential [14]. Only a very small percentage of WFPs are used on a large scale, while the majority of them are still overlooked by mainstream society [13]. Wild plants, due to their phyto-constituents, retain remarkable medicinal, pharmacological, and nutraceutical potential to cure diseases, and thus Lim [12] asserted that herbal infusions can add small quantities of essential micronutrients and vitamins to daily intake to help restore balance among nutrients and maintain health.
There are hundreds of field ethnobotanical studies that have been devoted to either food uses or medicinal uses of wild species, but to date the subject of food–medicines in cultural contexts is poorly researched even though studies have shown a general resurgence of interest in wild food plants for their nutritional and medicinal values so as to broaden the diversity of the human diet [15,16]. Kunakova et al. [17] argued that knowledge on the positive pharmacological potential of WFPs could provide a better opportunity to integrate these ingredients into the modern pharmacopeia to develop functional products with useful benefits.
North Pakistan is making important socio-ecological space for traditional ecological knowledge of wild food and medicinal resources. Several ethno-medicinal studies have been carried out in the region over the last decade [18,19,20,21]. More recently, a body of literature on wild food plant knowledge has also emerged [22,23]. Nevertheless, the subject of food–medicines is a relatively new subject in local and cultural contexts, as in many other parts of the world. The ethnobotanical literature has shown that the cultural knowledge of food–medicines retained by local communities in the region could provide an effective platform for devising innovative strategies and policy inferences in future healthcare interventions. Therefore, the overarching aim of this study was to document WFPs along with their folk medicinal uses in order to produce baseline data for further scientific discoveries related to food-based medicine.
The current study was undertaken in Mastuj Valley, Chitral District, North Pakistan. It is a remote mountain valley that has never been investigated with regard to WFPs and other wild food resources. This is the first study to record the biocultural food heritage of WFPs along with their medicinal importance. The specific objectives of this study were:
1.
To document the culinary and medicinal uses of WFPs gathered among the local communities;
2.
To cross-check the findings of the current study with previous ethnobotanical studies conducted in nearby regions and to research the ethnopharmacological relevance of the recorded plant taxa.
The current study represents the very first attempt at gathering an important store of information on local biocultural heritage from the remote Mastuj Valley focusing on wild food plants and their perceived medicinal properties.

2. Materials and Methods

2.1. Study Area and Studied Community

The study area is part of the Hindukush Mountain range and is located at the extreme north of Chitral District (Figure 1). The area is populated by the Kho people, who speak Khowar, the dominant linguistic group in Chitral. The Kho community has been living in the area for centuries. They are horticulturists that also rear livestock, and many individuals earn their livelihood from diverse types of off-farm activities. They have a long-term, inextricable link with nature and local resources, and they have amassed a remarkable store of traditional ecological and cultural knowledge on the local ecology and environment.

2.2. Field Study

An ethnobotanical survey was carried out in the month of June 2021 at different study sites across the valley. The main purpose of the study was to record the food and medicinal uses of wild plant taxa, commonly known as WFPs, and mushrooms among the local communities. A total of 30 people, with deep knowledge of and longtime experience with WFPs, were interviewed for the study. The interviewees were selected among middle-aged and elderly individuals (range: 50 to 72 years old), including local farmers and shepherds, as they retain sufficient knowledge of local wild food and medicinal resources. We only conducted interviews with men as there were socio-cultural constraints regarding the interviewing of women in the study area. Prior to each interview, we obtained verbal consent from each participant to record and publish their knowledge. The interviews were conducted in the Urdu language, and we employed the services of a local translator to avoid any difficulty in communicating with the participants. Throughout the study, recommendations given by the International Society of Ethnobiology were strictly followed [24]. The detailed interviews with study participants focused on gathered WFPs, i.e., plants used as cooked vegetables, snacks, recreational teas, and seasoning. We also inquired about the uses of WFPs in lacto-fermentation processes and dairy products. After detailed discussions on food uses, we focused on documenting the medicinal uses of the recorded taxa. We recorded the local names of each taxon in the Kho language, which were revised multiple times, by asking different elderly individuals to avoid any errors in local nomenclature. Qualitative ethnographic data were obtained through direct observations and in some cases through open-ended questions. At the end of the survey, recorded plant taxa were gathered, and the collected specimens were identified by the third author with the help of the Flora of Pakistan [25,26,27,28]. The specimens were assigned voucher numbers and incorporated into herbaria, subsequently submitted to the Department of Botany, University of Swat, Khyber Pakhtunkhwa, Pakistan. The scientific nomenclature of each taxon was verified through The Plant List database [29] and family assignments were consistent with the Angiosperm Phylogeny Website [30]. The Index Fungorum [31] was utilized to cross-check fungal nomenclature. The study was approved by the Ethics Committee of the University of Gastronomic Sciences, Pollenzo, Italy.

2.3. Data Analysis

The gathered data were entered into an MS Excel spreadsheet. The recorded WFPs were categorized into three groups based on folk uses, which were then visually displayed in a proportional Venn diagram drawn using free software (http://bioinformatics.psb.ugent.be/webtools/Venn/, accessed on 18 August 2021). We also compared our newly obtained data with the existing ethnobotanical literature of Pakistan to cross-check the quoted food and medicinal uses of the recorded WFPs with previous field studies. Moreover, we also researched the pharmacological potential of the recorded WFPs via peer-reviewed scientific literature on PubMed.

3. Results

3.1. Wild Food Plants and Their Uses

The use of a total of 43 taxa belonging to 25 families was recorded (Table 1). The WFPs most frequently quoted as wild vegetables were Amaranthus hybridus, Capparis spinosa, Chenopodium album, Eremurus stenophyllus, Ferula hindukushensis, Portulaca quadrifida, and Rumex dentatus. The majority of plants, which were largely harvested in their early growth stages, were used as cooked vegetables (24 taxa, e.g., Amaranthus hybridus, Capparis spinosa, Chenopodium album, Eremurus stenophyllus, Portulaca quadrifida) and consumed as raw snacks (15 taxa, e.g., Berberis parkeriana, Chenopodium foliosum, Cotoneaster nummularius, Crataegus songarica, Elaeagnus angustifolia). Among the reported plants, a few were cited for making teas. Leaves (22 taxa) were frequently used in culinary preparations.
We documented a total of 50 food uses for the recorded WFPs in which 35 use reports comprise 70% of all food uses, and these uses were mentioned by more than half of the informants, indicating a large store of knowledge still retained by the local inhabitants. Besides the food uses recorded for the 43 WFP taxa, 42 taxa were also mentioned by the study participants as either functional food or medicinal food, which we categorized into three groups (Figure 2) purely on the basis of the processing and preparation of these wild ingredients. We observed a huge overlap of use reports that were commonly cited as useful both for food and medicine, i.e., plants were consumed as food to obtain the target medicinal action, and these taxa are known as food–medicines or medicinal foods. The use reports that did not overlap represent divergences in the form of their processing and preparation, i.e., without any relationships between uses in these two domains for any targeted health actions. Wild food plants were mostly used to treat digestive issues (16 taxa, e.g., Amaranthus hybridus, Carum carvi, Convolvulus arvensis, Ferula hindukushensis, Lepyrodiclis holosteoides, and Mentha longifolia), followed by cardiovascular problems (four taxa, e.g., Ferula hindukushensis) and increased blood (four taxa, e.g., Crataegus songarica). Of the quoted taxa, nine plants were used or considered good for more than one ailment, i.e., Berberis parkeriana, Capparis spinosa, Elaeagnus rhamnoides, Ferula hindukushensis, Medicago sativa, Mentha longifolia, Nasturtium officinale, Rheum ribes, and Rumex dentatus. Moreover, 29 taxa were used for a single health problem. The most common health issue among gastric problems was constipation, which was frequently treated with green vegetables including Amaranthus hybridus, Capsella bursa-pastoris, Chenopodium album, Chenopodium murale, and Malva neglecta.
The link between disease and food has been widely recognized as the foundation of preventive nutrition. Over the last few decades, there have been other examples in Europe of wild plant species that have been introduced from other continents, such as Africa, e.g., Aspalathus linearis (Burm.f.) R. Dahlgren and to a lesser extent Cyclopia sp. or Hoodia gordonii (Masson) Sweet ex Decne., to stimulate appetite. Similarly, other exotic fruits rich in vitamin C, such as Malpighia glabra L. from South America, are now marketed as food supplements [88]. We also recorded four taxa that were considered as healthy foods without targeting any particular health problem. Elaborating on the concept of healthy food, a 70-year-old man explained:
“In the past we gathered WFPs from the mountains and consumed them as these plants gave us more power and energy. We were healthy, more capable of working hard and climbing mountains. But now with the arrival of bazaars and local markets, everyone can find Bazaari vegetables and people don’t bother to gather these WFPs from the mountains, except for a few individuals that graze their animals or hunt. WFPs are more powerful than cultivated ones and old people prefer these much more”.
Wild food plants have been recognized as “depurative” and they are a kind of “folk functional food” as defined by Pruess [89]; that is, food that has nutritional value or is eaten for pleasure but which also has beneficial effects on health. We also carried out a literature survey to determine the medicinal uses of these taxa and found a total of 27 food taxa that were reported as food-medicines in nearby regions (Table 1). Similarly, the literature also revealed 13 taxa among the recorded plants that were used both in the food and medicinal domains without any relationship among their respective uses. Looking at the overall results, we can presume that many plants were utilized in a multifunctional way, forming a complex web of relationships as presented in Table 1.
We observed that medicinal knowledge was popular among very few participants while culinary uses of the recorded taxa were widely known in the local communities, especially among elderly individuals. In the area, both men and women were involved in the gathering of WFPs: men generally collected the WFPs in the mountains while women handled the processing and cooking. Therefore, both men and women have deep cultural knowledge of these natural resources. During the study, we were not allowed to interview women, but we observed that sometimes male informants asked their wives to help recall some information related to WFPs. As the cooking is performed by women, they may have a deeper attachment to, and thus stronger memories of, WFPs compared to men. In the study area, during the last twenty years, the dependency of local communities on WFPs has decreased tremendously due to the invasion of local food markets and the prevalence of allopathic medicines. However, we encountered people who are still practicing pastoralism and hunting, engaged in the gathering and consumption of WFPs. It is worth mentioning that the rapid economic evolution and change in social life of the studied community have significantly interrupted the intergenerational transmission of traditional knowledge, although middle-aged and elderly individuals possess abundant cultural knowledge of the local resources. We observed in some homes some important WFPs (Figure 3) that were gathered from pastures located at higher elevations in the mountains as they were considered useful for their medicinal properties. We were told that younger people, who tend to live in cities, were not familiar with the food value of most WFPs, and they are more interested in receiving a modern education.
The wild vegetables that have nearly disappeared from the local food system include Capsella bursa-pastoris, Convolvulus arvensis, Descurainia Sophia, Lepyrodiclis holosteoides, Malva neglecta, Medicago sativa, Plantago lanceolata, Portulaca quadrifida, Rumex dentatus, Silene conoidea, and Taraxacum campylodes. The disappearance of these taxa is linked not only to globalization and social change but also to environmental change. Studies have shown that environmental change in the Hindukush and Himalayan region has greatly impacted the local natural resources. Measuring the LEK in West Pakistan, Abbas et al. [90] reported that the cultural knowledge of WFPs was partially eroded as they found that the majority of quoted plants were mentioned by only one third of the participants. However, the TEK of WFPs is still alive in both the memory and current practices of the local inhabitants; for example, communities living around Takht-e-Sulaiman Hills, NW Pakistan [91], as well as the Hindu Kush mountains in North Pakistan [23], have retained important traditional knowledge of WFPs. The popularity of WFPs as food–medicines has also been recognized in various communities living in North Pakistan. In a nearby region, Aziz et al. [22] identified some important plant species used in both food and medicine, for instance, Allium carolinianum was generally preferred by elderly individuals for treating joint pain. Capparis spinosa was considered an important economic plant gathered by the local communities and is well known for treating liver problems, as well as diabetes, hepatitis, cough, cold, fever, and malaria. Shi et al. [92] reported that in China, Korea, Japan, and other countries of Southeast Asia, medicinal plants are frequently used in both everyday foods and functional foods for medical purposes. Heinrich [93] states that the blurring of the food and medicine interface is a common theme across multiple contexts and cultures.

3.2. Pharmacological Effects of the WFPs Recorded in the Study

Research has shown that wild plant species have enormous capacity for certain pharmacological effects. The question of the continuum between food and medicine is of great interest to (ethno-)pharmacologists [94,95,96,97,98]. Pieroni et al. [99], for instance, reported that non-cultivated food species have strong antioxidant potential. Looking at the pharmacological profile of the quoted WFPs, we found that a sufficient proportion of WFPs were not verifiable on PubMed, i.e., 22 medicinal use reports out of 54 use reports had some pharmacological properties that have already been studied. During the literature survey, we found twenty-five in vivo and twenty-four in vivo studies. Among the quoted plants, 12 taxa (Amaranthus hybridus, Capsella bursa-pastoris, Carum carvi, Chenopodium album, Convolvulus arvensis, Crataegus songarica, Malva neglecta, Medicago sativa, Mentha longifolia, Nasturtium officinale, Taraxacum campylodes, and Thymus linearis) have proven antioxidant potential (Table 1). These plant taxa were used to treat gastric and cardiovascular issues, for which antioxidant agents play a crucial role in the prevention of such health problems. Antioxidants have the potential to scavenge free radicals inside the human body, which is beneficial as the presence of free radicals can have injurious effects and cause serious ailments, such as inflammatory cardiovascular diseases, cancer, and cataracts [100]. Plants are considered an important source of natural antioxidants [101], while synthetic antioxidants have been reported to have serious physiological effects on the human body, and therefore scientists have emphasized that plant-derived natural antioxidants are important alternatives to synthetic ones [102]. In recent times, a notable trend has emerged in which dietary plants are utilized as therapeutic antioxidants to decrease the rate of chronic diseases across the globe [101]. It has been reported that there is an inverse relationship between the dietary intake of antioxidant-rich foods and medicinal plants and the incidence of human diseases. It is believed that natural antioxidants are much cheaper than synthetic ones and have no harmful effects on the human body. Many plants have been investigated for their antioxidant potential including potatoes, tomatoes, spinach, legumes, and a variety of vegetables [103], and many fruits have been found to have strong antioxidant potential, such as cherries, berries, citrus fruits, olives, and prunes. Similarly, black and green teas have been extensively studied for their antioxidant activity since they contain up to 30% phenolic compounds by dry weight [104,105]. This is the reason dietary intervention studies have been devoted to assessing the impacts of increasing consumption of antioxidant foods on cardiovascular disease risk [106]. It is equally important to isolate the various phytochemicals present in these different food and medicinal species so as to make them part of important food supplements that would have more positive impacts than synthetic food products. Natural antioxidants, particularly carotenoids and polyphenols, display a wide range of biological effects, including anti-aging, anti-inflammatory, anticancer, and anti-atherosclerosis, and thus the careful extraction and proper assessment of antioxidants from both the recorded food and medicinal plants are essential for exploring potential antioxidant sources and promoting their applications in functional foods, pharmaceuticals, and food additives [107]. In today’s world, the exploration of novel food supplements is crucial and, in this regard, systematic investigations of such plants, also known as food-medicinal plants, are providing an important opportunity, especially in isolated areas.

3.3. Inclusion of LEK of WFPs with Medicinal Properties in Educational Platforms

During this study, we observed that younger generations have become detached from the environment supporting the learning of LEK as they are more interested in obtaining a modern education. It is important to note that the rapid social change caused by modernization and globalization processes has made it imperative to revitalize cultural and traditional knowledge through proper educational programs. However, the revitalization of LEK in educational programs is a two-way street involving both relevant stakeholders as well as local communities to effectively transmit cultural knowledge of local ecological resources. We strongly believe that bringing LEK knowledge into actual classroom practices will not only promote biocultural heritage, but it will also help students to understand science-based local ecological experiences. Curriculum designers and relevant policy makers should pay serious attention to the erosion of LEK and try to involve ethnobiologists in designing a culturally ecologically responsive curriculum to give LEK and the local biocultural heritage of WFPs the chance to survive and evolve along with modern scientific knowledge. Proper incentives should be given to local knowledge holders in order to secure their services. It is strongly recommended that policy makers pay serious attention to the efficient revitalization, through educational programs, of the LEK that has been an important cultural element of local ecological communities for generations. It should be kept in mind that LEK plays an important role in managing the local environment and resources, and therefore the popularization of WFPs with health benefit properties could be a better option in the modern world. We suggest that LEK should be part of policy frameworks in future development programs because traditional knowledge has played a crucial role in organizing both local ecosystems and local subsistence economies. Similarly, medicinal plants have supported the healthcare system for centuries, and thus policy makers should involve ethnobiologists around the country to formulate better strategies to protect and practically revive LEK and local biocultural heritage through educational and development programs to achieve sustainable development goals [108].

4. Conclusions

The current study reported an important store of cultural knowledge of WFPs used as food–medicines among local communities residing in a remote valley of Chitral, North Pakistan. Most of the plants quoted in this study that were processed for culinary uses were also used for their perceived health benefits as they are commonly recognized as medicinal foods or food-medicines. Previous ethnobotanical studies conducted in nearby regions have also shown that most of the recorded food plant taxa have important medicinal uses for the local communities, thus strengthening and supporting the findings of the current research. A literature survey also indicated that about one third of the quoted plants have not been tested for their pharmacological potential and were not verifiable on PubMed as they have not been studied for their respective medicinal actions. It is important to note that biocultural heritage is gradually eroding due to rapid social change and urbanization processes. On the basis of the findings of the current study, we recommend verifying, through standard laboratory techniques, the pharmacological potential of WFPs for the preparation of better food and nutrition supplements to help combat some human diseases. We also emphasize the revitalization of WFP-centered local biocultural heritage through future development and educational programs, which could represent a timely response to the loss of LEK. Lastly, we also suggest that future research should focus on the traditional ethnobotanical knowledge held by women in the study area.

Author Contributions

A.P. and M.A.A. designed the theoretical framework of the research. M.A.A. planned the methodology and carried out the field study. Z.U. identified the ethnobotanical taxa. A.P., M.A.A. and M.A. analyzed the data and provided the cultural interpretation of the findings. M.A.A. drafted the first version of the manuscript, which was later commented on by R.S. along with A.P., and then M.A.A. thoroughly revised and finalized the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the University of Gastronomic Sciences, Pollenzo, Italy.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Acknowledgments

Special thanks are due to all study participants of the different groups who generously shared their knowledge.

Conflicts of Interest

The authors declare that they have no conflict of interest.

References

  1. Chatterjee, M. The Food of Healing. IIC Q. 1985, 12, 129–140. [Google Scholar]
  2. Etkin, N. Edible Medicines—An Ethnopharmacology of Food; The University of Arizona Press: Tucson, AZ, USA, 2006. [Google Scholar]
  3. Shrivastava, S.R.; Shrivastava, P.S.; Ramasamy, J. World Health Organization advocates for a healthy diet for all: Global perspective. J. Res. Med. Sci. 2016, 21, 44. [Google Scholar] [CrossRef]
  4. Willett, W.C.; Koplan, J.P.; Nugent, R.; Dusenbury, C.; Puska, P.; Gaziano, T.A. Prevention of chronic disease by means of diet and lifestyle changes. In Disease Control Priorities in Developing Countries, 2nd ed.; Jamison, D.T., Breman, J.G., Measham, A.R., Alleyne, G., Claeson, M., Evans, D.B., Jha, P., Mills, A., Musgrove, P., Eds.; Oxford University Press: New York, NY, USA, 2006. [Google Scholar]
  5. GBD 2017 Diet Collaborators. Health effects of dietary risks in 195 countries, 1990–2017: A systematic analysis for the Global Burden of Disease Study 2017. Lancet 2019, 393, 1958–1972. [Google Scholar] [CrossRef] [Green Version]
  6. Downer, S.; Berkowitz, S.A.; Harlan, T.S.; Olstad, D.L.; Mozaffarian, D. Food is medicine: Actions to integrate food and nutrition into healthcare. Br. Med. J. 2020, 369, 1–6. [Google Scholar] [CrossRef]
  7. Berkowitz, S.A.; Delahanty, L.M.; Terranova, J.; Steiner, B.; Ruazol, M.P.; Singh, R.; Shahid, N.N.; Wexler, D.J. Medically tailored meal delivery for diabetes patients with food insecurity: A randomized cross-over trial. J. Gen. Intern. Med. 2019, 34, 396–404. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Seligman, H.K.; Lyles, C.; Marshall, M.B.; Prendergast, K.; Smith, M.C.; Headings, A.; Bradshaw, G.; Rosenmoss, S.; Waxman, E. A pilot food bank intervention featuring diabetes-appropriate food improved glycemic control among clients in three states. Health Aff. 2015, 34, 1956–1963. [Google Scholar] [CrossRef] [Green Version]
  9. Bryce, R.; Guajardo, C.; Ilarraza, D.; Milgrom, N.; Pike, D.; Savoie, K.; Valbuena, F.; Miller-Matero, L.R. Participation in a farmers’ market fruit and vegetable prescription program at a federally qualified health center improves hemoglobin A1C in low income uncontrolled diabetics. Prev. Med. Rep. 2017, 7, 176–179. [Google Scholar] [CrossRef]
  10. Addis, G.; Urga, K.; Dikasso, D. Ethnobotanical study of edible wild plants in some selected districts of Ethiopia. Hum. Ecol. 2005, 33, 83–118. [Google Scholar] [CrossRef]
  11. Balemie, K.; Kebebew, F. Ethnobotanical study of wild edible plants in Derashe and Kucha Districts, South Ethiopia. J. Ethnobiol. Ethnomed. 2006, 2, 53. [Google Scholar] [CrossRef] [Green Version]
  12. Lim, T.K. Edible Medicinal and Non-Medicinal Plants; Springer: Dordrecht, The Netherland; Heidelberg, Germany; London, UK; New York, NY, USA, 2012. [Google Scholar]
  13. Shikov, A.N.; Tsitsilin, A.N.; Pozharitskaya, O.N.; Makarov, V.G.; Heinrich, M. Traditional and current food use of wild plants listed in the Russian Pharmacopoeia. Front. Pharmacol. 2017, 8, 841. [Google Scholar] [CrossRef]
  14. Gammerman, A.F.; Grom, I.I. Wild Medicinal Plants of the USSR; Medizina: Moscow, Russia, 1976. [Google Scholar]
  15. Flyman, M.V.; Afolayan, A.J. Proximate and mineral composition of the leaves of Momordica balsamina L.: An under-utilized wild vegetable in Botswana. Int. J. Food Sci. Nutr. 2007, 58, 419–423. [Google Scholar] [CrossRef] [PubMed]
  16. Afolayan, A.J.; Jimoh, F.O. Nutritional quality of some wild leafy vegetables in South Africa. Int. J. Food Sci. Nutr. 2009, 60, 424–431. [Google Scholar] [CrossRef] [PubMed]
  17. Kunakova, R.V.; Zainullin, R.A.; Abramova, L.M.; Anischenko, O.E. Food and Medicinal Plants in Functional Food Products; Gilem: Ufa, Russia, 2011. [Google Scholar]
  18. Abbas, Z.; Khan, S.M.; Alam, J.; Khan, S.W.; Abbasi, A.M. Medicinal plants used by inhabitants of the Shigar Valley, Baltistan region of Karakoram Range-Pakistan. J Ethnobiol. Ethnomed. 2017, 13, 53. [Google Scholar] [CrossRef]
  19. Abbas, Z.; Kousar, S.; Aziz, M.A.; Pieroni, A.; Aldosari, A.A.; Bussmann, R.W.; Raza, G.; Abbasi, A.M. Comparative assessment of medicinal plant utilization among Balti and Shina communities in the periphery of Deosai National Park, Pakistan. Biology 2021, 10, 434. [Google Scholar] [CrossRef] [PubMed]
  20. Islam, M.; Ahmad, I.; Akhtar, N.; Alam, J.; Razzaq, A.; Mohammad, K.; Mahmood, T.; Khan, F.U.; Khan, W.M.; Qamar, S.; et al. Medicinal plants resources of Western Himalayan Palas Valley, Indus Kohistan, Pakistan: Their uses and degrees of risk of extinction. Saudi J. Biol. Sci. 2021, 28, 3076–3093. [Google Scholar] [CrossRef]
  21. Bano, A.; Ahmad, M.; Hadda, T.B.; Saboor, A.; Sultana, S.; Zafar, M.; Khan, M.P.Z.; Arshad, M.; Ashraf, M.A. Quantitative ethnomedicinal study of plants used in the Skardu valley at high altitude of Karakoram-Himalayan range, Pakistan. J. Ethnobiol. Ethnomed. 2014, 10, 43. [Google Scholar] [CrossRef] [Green Version]
  22. Aziz, M.A.; Abbasi, A.M.; Ullah, Z.; Pieroni, A. Shared but threatened: The heritage of wild food plant gathering among different linguistic and religious groups in the Ishkoman and Yasin Valleys, North Pakistan. Foods 2020, 9, 601. [Google Scholar] [CrossRef]
  23. Aziz, M.A.; Ullah, Z.; Pieroni, A. Wild food plant gathering among Kalasha, Yidgha, Nuristani and Khowar speakers in Chitral, NW Pakistan. Sustainability 2020, 12, 9176. [Google Scholar] [CrossRef]
  24. International Society of Ethnobiology (ISE). Code of Ethics. 2008. Available online: www.ethnobiology.net/whatwe-do/coreprograms/ise-ethics-program/code-of-ethics/ (accessed on 1 August 2021).
  25. Ali, S.I.; Qaiser, M. (Eds.) Flora of Pakistan; University of Karachi: Karachi, Pakistan, 1993–2009. [Google Scholar]
  26. Nasir, E.; Ali, S.I. (Eds.) Flora of Pakistan; No. 132–190; University of Karachi: Karachi, Pakistan, 1980–1989. [Google Scholar]
  27. Nasir, E.; Ali, S.I. (Eds.) Flora of Pakistan; No. 191–193; University of Karachi: Karachi, Pakistan, 1989–1992. [Google Scholar]
  28. Nasir, E.; Ali, S.I. (Eds.) Flora of West Pakistan; No. 1–131; University of Karachi: Karachi, Pakistan, 1970–1979. [Google Scholar]
  29. The Plant List, Version 1, Published on the Internet. Available online: http://www.theplantlist.org/ (accessed on 1 August 2021).
  30. Stevens, P.F. Angiosperm Phylogeny Website, Version 14. 2017. Available online: http://www.mobot.org/MOBOT/research/APweb (accessed on 1 August 2021).
  31. Index Fungorum. 2020. Available online: http://www.indexfungorum.org/Names/Names.asp (accessed on 1 August 2021).
  32. Yatoo, M.I.; Dimri, U.; Gopalakrishnan, A.; Saxena, A.; Wani, S.A.; Dhama, K. In vitro and in vivo immunomodulatory potential of Pedicularis longiflora and Allium carolinianum in alloxan-induced diabetes in rats. Biomed. Pharmacother. 2018, 97, 375–384. [Google Scholar] [CrossRef]
  33. Aziz, M.A.; Ullah, Z.; Al-Fatimi, M.; De Chiara, M.; Sõukand, R.; Pieroni, A. On the Trail of an Ancient Middle Eastern Ethnobotany: Traditional Wild Food Plants Gathered by Ormuri Speakers in Kaniguram, NW Pakistan. Biology 2021, 10, 302. [Google Scholar] [CrossRef]
  34. Bibi, N. Profile of the medicinal and economic plants of Laspur Valley Chitral, Pakistan. Med. Aromat. Plants 2019, 8, 330. [Google Scholar] [CrossRef] [Green Version]
  35. Tang, Y.; Xiao, Y.; Tang, Z.; Jin, W.; Wang, Y.; Chen, H.; Yao, H.; Shan, Z.; Bu, T.; Wang, X. Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity. PeerJ 2019, 7, e7149. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Ali, H.; Qaiser, M. The ethnobotany of Chitral valley, Pakistan with particular reference to medicinal plants. Pak. J. Bot. 2009, 41, 2009–2041. [Google Scholar]
  37. Cicero, A.F.; Baggioni, A. Berberine and its Role in Chronic Disease. Adv. Exp. Med. Biol. 2016, 928, 27–45. [Google Scholar] [PubMed]
  38. Abd El-Wahab, A.E.; Ghareeb, D.A.; Sarhan, E.E.; Abu Serie, M.M.; El Demellawy, M.A. In vitro biological assessment of Berberis vulgaris and its active constituent, berberine: Antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects. BMC Complement. Altern. Med. 2013, 13, 218. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  39. Lin, K.; Liu, S.; Shen, Y.; Li, Q. Berberine attenuates cigarette smoke-induced acute lung inflammation. Inflammation 2013, 36, 1079–1086. [Google Scholar] [CrossRef] [PubMed]
  40. Yu, F.S.; Yang, J.S.; Lin, H.J.; Yu, C.S.; Tan, T.W.; Lin, Y.T.; Lin, C.C.; Lu, H.F.; Chung, J.G. Berberine inhibits WEHI-3 leukemia cells in vivo. In Vivo 2007, 21, 407–412. [Google Scholar]
  41. Cernáková, M.; Kost’álová, D.; Kettmann, V.; Plodová, M.; Tóth, J.; Drímal, J. Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium. BMC Complement. Altern. Med. 2002, 2, 2. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  42. Eddouks, M.; Lemhadri, A.; Michel, J.B. Hypolipidemic activity of aqueous extract of Capparis spinosa L. in normal and diabetic rats. J. Ethnopharmacol. 2005, 98, 345–350. [Google Scholar] [CrossRef]
  43. Lemhadri, A.; Eddouks, M.; Sulpice, T.; Burcelin, R. Anti-hyperglycaemic and anti-obesity effects of Capparis spinosa and Chamaemelum nobile aqueous extracts in HFD mice. Am. J. Pharmacol. Toxicol. 2007, 2, 106–110. [Google Scholar] [CrossRef] [Green Version]
  44. Huseini, H.F.; Hasani-Rnjbar, S.; Nayebi, N.; Heshmat, R.; Sigaroodi, F.K.; Ahvazi, M.; Alaei, B.A.; Kianbakht, S. Capparis spinosa L. (caper) fruit extract in treatment of type 2 diabetic patients: A randomized double-blind placebo-controlled clinical trial. Complement. Ther. Med. 2013, 21, 447–452. [Google Scholar] [CrossRef]
  45. Ali, Z.N.; Eddouks, M.; Michel, J.B.; Sulpice, T.; Hajji, L. Cardiovascular effect of Capparis spinosa aqueous extract. Part III: Antihypertensive effect in spontaneously hypertensive rats. Am. J. Pharmacol. Toxicol. 2007, 2, 111–115. [Google Scholar] [CrossRef]
  46. Boga, C.; Forlani, L.; Calienni, R.; Hindley, T.; Hochkoeppler, A.; Tozzi, S.; Zanna, N. On the antibacterial activity of roots of Capparis spinosa L. Nat. Prod. Res. 2011, 25, 417–421. [Google Scholar] [CrossRef] [PubMed]
  47. El Azhary, K.; Tahiri Jouti, N.; El Khachibi, M.; Moutia, M.; Tabyaoui, I.; El Hou, A.; Achtak, H.; Nadifi, S.; Habti, N.; Badou, A. Anti-inflammatory potential of Capparis spinosa L. in vivo in mice through inhibition of cell infiltration and cytokine gene expression. BMC Complement. Altern. Med. 2017, 17, 81. [Google Scholar]
  48. Gadgoli, C.; Mishra, S.H. Antihepatotoxic activity of p-methoxy benzoic acid from Capparis spinosa. J. Ethnopharmacol. 1999, 66, 187–192. [Google Scholar] [CrossRef]
  49. Kubínová, R.; Spačková, V.; Svajdlenka, E.; Lučivjanská, K. Antioxidační aktivita extraktů a HPLC analýza flavonoidů Capsella bursa-pastoris (L.) Medik [Antioxidant activity of extracts and HPLC analysis of flavonoids from Capsella bursa-pastoris (L.) Medik]. Ceska. Slov. Farm. 2013, 62, 174–176. [Google Scholar]
  50. Bourgou, S.; Rebey, I.B.; Dakhlaoui, S.; Msaada, K.; Tounsi, M.S.; Ksouri, R.; Fauconnier, M.L.; Hamrouni-Sellami, I. Green extraction of oil from Carum carvi seeds using bio-based solvent and supercritical fluid: Evaluation of its antioxidant and anti-inflammatory activities. Phytochem. Anal. 2020, 31, 37–45. [Google Scholar] [CrossRef]
  51. Hajlaoui, H.; Arraouadi, S.; Noumi, E.; Aouadi, K.; Adnan, M.; Khan, M.A.; Kadri, A.; Snoussi, M. Antimicrobial, antioxidant, anti-acetylcholinesterase, antidiabetic, and pharmacokinetic properties of Carum carvi L. and Coriandrum sativum L. essential oils alone and in combination. Molecules 2021, 26, 3625. [Google Scholar] [CrossRef]
  52. Amodeo, V.; Marrelli, M.; Pontieri, V.; Cassano, R.; Trombino, S.; Conforti, F.; Statti, G. Chenopodium album L. and Sisymbrium officinale (L.) Scop.: Phytochemical content and in vitro antioxidant and anti-inflammatory potential. Plants 2019, 8, 505. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Azman, N.A.M.; Gallego, M.G.; Juliá, L.; Fajari, L.; Almajano, M. The effect of Convolvulus arvensis dried extract as a potential antioxidant in food models. Antioxidants 2015, 4, 170–184. [Google Scholar] [CrossRef] [Green Version]
  54. Ganie, S.A.; Dar, A.T.; Zargar, S.; Bhat, A.H.; Dar, K.B.; Masood, A.; Zargar, M.A. Crataegus songarica methanolic extract accelerates enzymatic status in kidney and heart tissue damage in albino rats and it’s in vitro cytotoxic activity. Pharm. Biol. 2016, 54, 1246–1254. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  55. Younis, W.; Alamgeer; Schini-Kerth, V.B.; da Silva, D.B.; Junior, A.G.; Bukhari, I.A.; Assiri, A.M. Role of the NO/cGMP pathway and renin-angiotensin system in the hypotensive and diuretic effects of aqueous soluble fraction from Crataegus songarica K. Koch. J. Ethnopharmacol. 2020, 249, 112400. [Google Scholar] [CrossRef]
  56. Nimrouzi, M.; Zarshenas, M.M. Phytochemical and pharmacological aspects of Descurainia sophia Webb ex Prantl: Modern and traditional applications. Avicenna J. Phytomed. 2016, 6, 266–272. [Google Scholar] [PubMed]
  57. Gong, G.; Guan, Y.Y.; Zhang, Z.L.; Rahman, K.; Wang, S.J.; Zhou, S.; Luan, X.; Zhang, H. Isorhamnetin: A review of pharmacological effects. Biomed. Pharmacother. 2020, 128, 110301. [Google Scholar] [CrossRef]
  58. Pundir, S.; Garg, P.; Dviwedi, A.; Ali, A.; Kapoor, V.K.; Kapoor, D.; Kulshrestha, S.; Lal, U.R.; Negi, P. Ethnomedicinal uses, phytochemistry and dermatological effects of Hippophae rhamnoides L.: A review. J. Ethnopharmacol. 2021, 266, 113434. [Google Scholar] [CrossRef]
  59. Michel, T.; Destandau, E.; Le Floch, G.; Lucchesi, M.E.; Elfakir, C. Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn (Hippophaë rhamnoides L.) leaf, stem, root and seed. Food Chem. 2012, 131, 754–760. [Google Scholar] [CrossRef]
  60. Olas, B.; Kontek, B.; Malinowska, P.; Żuchowski, J.; Stochmal, A. Hippophae rhamnoides L. fruits reduce the oxidative stress in human blood platelets and plasma. Oxid. Med. Cell. Longev. 2016, 2016, 4692486. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  61. Guo, R.; Guo, X.; Li, T.; Fu, X.; Liu, R.H. Comparative assessment of phytochemical profiles, antioxidant and antiproliferative activities of Sea buckthorn (Hippophaë rhamnoides L.) berries. Food Chem. 2017, 221, 997–1003. [Google Scholar] [CrossRef]
  62. Pang, X.; Zhao, J.; Zhang, W.; Zhuang, X.; Wang, J.; Xu, R.; Xu, Z.; Qu, W. Antihypertensive effect of total flavones extracted from seed residues of Hippophae rhamnoides L. in sucrose-fed rats. J. Ethnopharmacol. 2008, 117, 325–331. [Google Scholar] [CrossRef]
  63. Chauhan, A.S.; Negi, P.S.; Ramteke, R.S. Antioxidant and antibacterial activities of aqueous extract of Seabuckthorn (Hippophae rhamnoides) seeds. Fitoterapia 2007, 78, 590–592. [Google Scholar] [CrossRef]
  64. Shipulina, L.D.; Tolkachev, O.N.; Krepkova, L.V.; Bortnikova, V.V.; Shkarenkov, A.A. Anti-viral, anti-microbial and toxicological studies on sea buckthorn (Hippophae rhamnoides). In Seabuckthorn (Hippophae L.). A Multipurpose Wonder Plant; Singh, V., Yang, B., Kallio, H., Bala, M., Sawhney, R.C., Gupta, R.K., Mörsel, J.T., Rongsen, L., Tolkachev, O.N., Eds.; Daya Publishing House: New Delhi, India, 2005; pp. 471–483. [Google Scholar]
  65. Gupta, N.; Sharma, S.K.; Rana, J.C.; Chauhan, R.S. Expression of flavonoid biosynthesis genes vis-à-vis rutin content variation in different growth stages of Fagopyrum species. J. Plant Physiol. 2011, 168, 2117–2123. [Google Scholar] [CrossRef] [PubMed]
  66. Traber, M.G. Vitamin E inadequacy in humans: Causes and consequences. Adv. Nutr. 2014, 5, 503–514. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  67. Xing, J.; Yang, B.; Dong, Y.; Wang, B.; Wang, J.; Kallio, H.P. Effects of sea buckthorn (Hippophaë rhamnoides L.) seed and pulp oils on experimental models of gastric ulcer in rats. Fitoterapia 2002, 73, 644–650. [Google Scholar] [CrossRef]
  68. Hamułka, J.; Górnicka, M.; Sulich, A.; Frąckiewicz, J. Weight loss program is associated with decrease α-tocopherol status in obese adults. Clin. Nutr. 2019, 38, 1861–1870. [Google Scholar] [CrossRef]
  69. Li, T.S.C.; Beveridge, T.H.J. Sea Buckthorn (Hippophae rhamnoides L.): Production and Utilization, 1st ed.; NRC Research Press: Ottawa, ON, Canada, 2003. [Google Scholar]
  70. Ranard, K.M.; Erdman, J.W., Jr. Effects of dietary RRR α-tocopherol vs all-racemic α-tocopherol on health outcomes. Nutr. Rev. 2018, 76, 141–153. [Google Scholar] [CrossRef]
  71. Xu, H.; Hao, Q.; Yuan, F.; Gao, Y. Nonenzymatic browning criteria to sea buckthorn juice during thermal processing. J. Food Process Eng. 2015, 38, 67–75. [Google Scholar] [CrossRef]
  72. Hou, D.D.; Di, Z.H.; Qi, R.Q.; Wang, H.X.; Zheng, S.; Hong, Y.X.; Guo, H.; Chen, H.D.; Gao, X.H. Sea buckthorn (Hippophaë rhamnoides L.) oil improves atopic dermatitis-like skin lesions via inhibition of NF-κB and STAT1 activation. Skin Pharmacol. Physiol. 2017, 30, 268–276. [Google Scholar] [CrossRef]
  73. Khan, B.A.; Akhtar, N.; Mahmood, T.; Shoaib, H.M.; Qayum, M.; Saeed, T. Effects of antioxidants and flavonoids of sea buckthorn on skin whitening and skin erythema. Asian J. Chem. 2011, 23, 903–906. [Google Scholar]
  74. Rabiei, K.; Ebrahimzadeh, M.A.; Saeedi, M.; Bahar, A.; Akha, O.; Kashi, Z. Effects of a hydroalcoholic extract of Juglans regia (walnut) leaves on blood glucose and major cardiovascular risk factors in type 2 diabetic patients: A double-blind, placebo-controlled clinical trial. BMC Complement. Altern. Med. 2018, 18, 206. [Google Scholar] [CrossRef]
  75. Samoylenko, V.; Dunbar, D.C.; Gafur, M.A.; Khan, S.I.; Ross, S.A.; Mossa, J.S.; El-Feraly, F.S.; Tekwani, B.L.; Bosselaers, J.; Muhammad, I. Antiparasitic, nematicidal and antifouling constituents from Juniperus berries. Phytother. Res. 2008, 22, 1570–1576. [Google Scholar] [CrossRef] [PubMed]
  76. Güder, A.; Korkmaz, H. Evaluation of in-vitro antioxidant properties of hydroalcoholic solution extracts Urtica dioica L., Malva neglecta Wallr. and their mixture. Iran. J. Pharm. Res. 2012, 11, 913–923. [Google Scholar]
  77. Krakowska, A.; Rafińska, K.; Walczak, J.; Kowalkowski, T.; Buszewski, B. Comparison of various extraction techniques of Medicago sativa: Yield, antioxidant activity, and content of phytochemical constituents. J. AOAC Int. 2017, 100, 1681–1693. [Google Scholar] [CrossRef] [PubMed]
  78. Farzaei, M.H.; Bahramsoltani, R.; Ghobadi, A.; Farzaei, F.; Najafi, F. Pharmacological activity of Mentha longifolia and its phytoconstituents. J. Tradit. Chin. Med. 2017, 37, 710–720. [Google Scholar] [CrossRef]
  79. Li, W.; Cai, Z.N.; Mehmood, S.; Liang, L.L.; Liu, Y.; Zhang, H.Y.; Chen, Y.; Lu, Y.M. Anti-inflammatory effects of Morchella esculenta polysaccharide and its derivatives in fine particulate matter-treated NR8383 cells. Int. J. Biol. Macromol. 2019, 129, 904–915. [Google Scholar] [CrossRef] [PubMed]
  80. Bahramikia, S.; Yazdanparast, R. Antioxidant efficacy of Nasturtium officinale extracts using various in vitro assay systems. J. Acupunct. Meridian Stud. 2010, 3, 283–290. [Google Scholar] [CrossRef]
  81. Monforte, M.T.; Smeriglio, A.; Germanò, M.P.; Pergolizzi, S.; Circosta, C.; Galati, E.M. Evaluation of antioxidant, anti-inflammatory, and gastroprotective properties of Rubus fruticosus L. fruit juice. Phytother. Res. 2018, 32, 1404–1414. [Google Scholar] [CrossRef]
  82. Zia-Ul-Haq, M.; Riaz, M.; De Feo, V.; Jaafar, H.Z.E.; Moga, M. Rubus Fruticosus L.: Constituents, Biological Activities and Health Related Uses. Molecules 2014, 19, 10998–11029. [Google Scholar] [CrossRef] [Green Version]
  83. Kuete, V. Physical, hematological, and histopathological signs of toxicity induced by African medicinal plants. In Toxicological Survey of African Medicinal Plants; Elsevier: Amsterdam, The Netherlands, 2014; pp. 635–657. [Google Scholar]
  84. Jainu, M.; Devi, C.S. Antiulcerogenic and ulcer healing effects of Solanum nigrum (L.) on experimental ulcer models: Possible mechanism for the inhibition of acid formation. J. Ethnopharmacol. 2006, 104, 156–163. [Google Scholar] [CrossRef]
  85. Ignat, M.V.; Coldea, T.E.; Salanță, L.C.; Mudura, E. Plants of the spontaneous flora with beneficial action in the management of diabetes, hepatic disorders, and cardiovascular disease. Plants 2021, 10, 216. [Google Scholar] [CrossRef]
  86. Karakuş, A.; Değer, Y.; Yıldırım, S. Protective effect of Silybum marianum and Taraxacum officinale extracts against oxidative kidney injuries induced by carbon tetrachloride in rats. Ren. Fail. 2017, 39, 1–6. [Google Scholar] [CrossRef] [Green Version]
  87. Jin, Y.R.; Jin, J.; Piao, X.X.; Jin, N.G. The effect of Taraxacum officinale on gastric emptying and smooth muscle motility in Rodents. Neurogastroenterol Motil. 2011, 23, 766-e333. [Google Scholar] [CrossRef]
  88. Franz, C.; Chizzola, R.; Novak, J.; Sponza, S. Botanical species being used for manufacturing plant food supplements (PFS) and related products in the EU member states and selected third countries. Food Funct. 2011, 2, 720–730. [Google Scholar] [CrossRef]
  89. Pruess, A. Characterization of functional food. Dtsch. Lebensm.-Rundsch. 1999, 95, 468–472. [Google Scholar]
  90. Abbas, W.; Hussain, W.; Badshah, L.; Hussain, K.; Pieroni, A. Traditional wild vegetables gathered by four religious groups in Kurram District, Khyber Pakhtunkhwa, North-West Pakistan. Genet. Resour. Crop. Evol. 2020, 67, 1521–1536. [Google Scholar] [CrossRef]
  91. Ahmad, K.; Pieroni, A. Folk knowledge of wild food plants among the tribal communities of Takht-e-Sulaiman Hills, North-West Pakistan. J. Ethnobiol. Ethnomed. 2016, 12, 17. [Google Scholar] [CrossRef] [Green Version]
  92. Shi, J.; Ho, C.T.; Shahidi, F. (Eds.) Functional Foods of the East, 1st ed.; CRC Press: Boca Raton, FL, USA; London, UK; New York, NY, USA, 2010. [Google Scholar]
  93. Heinrich, M. Food-herbal medicine interface. In Encyclopedia of Food and Health; Trugo, L., Finglas, P., Caballero, B., Eds.; Elsevier: Oxford, UK, 2016; pp. 94–98. [Google Scholar]
  94. Etkin, N.L.; Ross, P.J. Food as medicine and medicine as food: An adaptive framework for the interpretation of plant utilization among the Hausa of Northern Nigeria. Soc. Sci. Med. 1982, 16, 1559–1573. [Google Scholar] [CrossRef]
  95. Leonti, M. The co-evolutionary perspective of the food-medicine continuum and wild gathered and cultivated vegetables. Genet. Resour. Crop Evol. 2012, 59, 1295–1302. [Google Scholar] [CrossRef]
  96. Valussi, M.; Scire, A.S. Quantitative ethnobotany and traditional functional foods. Nutrafoods 2012, 11, 85–93. [Google Scholar] [CrossRef]
  97. Jennings, H.M.; Merrell, J.; Thompson, J.L.; Heinrich, M. Food or medicine? The food-medicine interface in households in Sylhet. J. Ethnopharmacol. 2015, 167, 97–104. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  98. Alarcón, R.; Pardo-de-Santayana, M.; Priestley, C.; Morales, R.; Heinrich, M. Medicinal and local food plants in the south of Alava (Basque Country, Spain). J. Ethnopharmacol. 2015, 176, 207–224. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  99. Pieroni, A.; Janiak, V.; Dürr, C.M.; Lüdeke, S.; Trachsel, E.; Heinrich, M. In vitro antioxidant activity of non-cultivated vegetables of ethnic Albanians in southern Italy. Phytother. Res. 2002, 16, 467–473. [Google Scholar] [CrossRef] [PubMed]
  100. Poljsak, B.; Šuput, D.; Milisav, I. Achieving the balance between ROS and antioxidants: When to use the synthetic antioxidants. Oxid. Med. Cell. Longev. 2013, 2013, 956792. [Google Scholar] [CrossRef] [PubMed]
  101. Lobo, V.; Patil, A.; Phatak, A.; Chandra, N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn. Rev. 2010, 4, 118–126. [Google Scholar] [CrossRef] [Green Version]
  102. Papas, A.M. Diet and antioxidant status. Food Chem. Toxicol. 1999, 37, 999–1007. [Google Scholar] [CrossRef]
  103. Furuta, S.; Nishiba, Y.; Suda, I. Fluorometric assay for screening antioxidative activities of vegetables. J. Food Sci. 1997, 62, 526–528. [Google Scholar] [CrossRef]
  104. Wang, H.; Cao, G.; Prior, R.L. Total antioxidant capacity of fruits. J. Agric. Food Chem. 1996, 44, 701–705. [Google Scholar] [CrossRef]
  105. Lin, J.K.; Lin, C.H.; Ling, Y.C.; Lin-Shian, S.Y.; Juan, I.M. Survey of catechins, gallic acid and methylxanthines in green, oolong, pu-erh and black teas. J. Agric. Food Chem. 1998, 46, 3635–3642. [Google Scholar] [CrossRef]
  106. Kasote, D.M.; Katyare, S.S.; Hegde, M.V.; Bae, H. Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int. J. Biol. Sci. 2015, 11, 982–991. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  107. Xu, D.P.; Li, Y.; Meng, X.; Zhou, T.; Zhou, Y.; Zheng, J.; Zhang, J.-J.; Li, H.-B. Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. Int. J. Mol. Sci. 2017, 18, 96. [Google Scholar] [CrossRef]
  108. Sõukand, R.; Kalle, R.; Fontefrancesco, M.F.; Pieroni, A. Building a safety buffer for European food security: The role of small-scale food production and local ecological and gastronomic knowledge in light of COVID-19 [version 1; peer review: 2 approved with reservations]. Open Res. Eur. 2021, 1, 10. [Google Scholar] [CrossRef]
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Figure 1. Location of the study area in Pakistan.
Figure 1. Location of the study area in Pakistan.
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Figure 2. Venn diagram showing the different uses of locally gathered WFPs in the study area.
Figure 2. Venn diagram showing the different uses of locally gathered WFPs in the study area.
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Figure 3. Plants found in some homes in the study area; (A,C) local informants explaining the uses of WFPs (B) Ferula hindukushensis and (D) Rheum ribes.
Figure 3. Plants found in some homes in the study area; (A,C) local informants explaining the uses of WFPs (B) Ferula hindukushensis and (D) Rheum ribes.
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Table
Table 1. WFPs used as food–medicines, along with their pharmacological properties, reported among local communities living in North Pakistan.
Table 1. WFPs used as food–medicines, along with their pharmacological properties, reported among local communities living in North Pakistan.
Botanical Taxon; Family;
Botanical Voucher Specimen Code		Recorded Local
Name		Parts UsedRecorded Local Food UsesPerceived Medicinal
Uses		Medicinal Uses Recorded in Nearby Regions, NW PakistanPharmacological Potential
Allium carolinianum DC.;
Amaryllidaceae; SWAT005988		LatrukLeavesCooked ***Hepatitis **Joint pain [22].In vivo: immunomodulatory potential [32].
Amaranthus hybridus L.; Amaranthaceae;
SWAT005470		Krui shakhLeavesCooked ***Constipation ***Vermicide, tonic [33]. Young leaves are used as vegetables, which are a laxative [34].In vitro: antioxidant activity [35].
Astragalus psilocantros L.; Fabaceae; SWAT005995GarmenzoStemInternal part of stem: raw snack *Internal soft part of the stem is put in the fire and then consumed to treat rheumatism *.--
Berberis parkeriana C.K. Schneid.;
Berberidaceae; SWAT005491		ChowengLeaves, FruitFruit: raw snack ***,
Leaves: Chatni ***		The fruit is considered a blood purifier ***.
A decoction of the root is used to reduce prolonged fever *.		The fruits are crushed, boiled in water, and then used to reduce fever [36]. Leaves and fruits are collected and juice is extracted and filtered, which can then be taken orally for the treatment of typhoid, jaundice, dyspepsia, blood purification, and muscular pain [34].In vivo: anti-inflammatory and antioxidant activities. In animal models, berberine has neuroprotective and cardiovascular protective effects. In humans, its lipid-lowering and insulin-resistance improving actions have clearly been demonstrated in numerous randomized clinical trials [37].
In vitro: anti-oxidant [38],
In vivo: anti-inflammatory [39],
In vivo: anti-tumor [40],
In vivo: anti-mutagenic [41],
In vitro: anti-diabetic [38] properties of berberine.
Capparis spinosa L.; Capparaceae;
SWAT005794		KaveerFruitFruit: vegetable ***,
Flower: extract is used in seasoning ***		Liver problems ***, typhoid *, and pneumonia *The cooked vegetable is considered useful in treating liver problems, diabetes, hepatitis, cough, cold, fever, and malaria [22]. Floral buds are collected, dried, mashed with wheat flour, and cooked to prepare an aqueous extract called Kavirough, which is effective for the treatment of abdominal pain, malaria, and typhoid [34].In vivo: anti-diabetic [42,43,44],
In vivo: anti-hypertensive [45],
In vitro: antimicrobial [46],
In vivo: anti-inflammatory [47],
In vivo: antihepatotoxic [48].
Capsella bursa-pastoris (L.) Medik.; Brassicaceae; SWAT005997PalakAerial partsCooked ***Constipation *-In vitro: antioxidant potential [49].
Carum carvi L.; Apiaceae; SWAT005966, SWAT005981HojoojFruit or seedsTea ***, seasoning ***, used in yogurt or dough and eaten to cool the brain *Gastric problems ***Seeds are boiled; herbal tea is made and is used for nausea and stomachache [34].In vitro: antioxidant, anti-inflammatory potential [50].
In vitro: antimicrobial, anti-acetylcholinesterase, and antidiabetic [51].
Chenopodium album L.; Amaranthaceae;
SWAT005499		KonakhLeavesCooked ***Constipation ***Leaves are served as vegetables for bowel disorders, as a laxative for constipation [34].In vivo: antioxidant and anti-inflammatory potential [52].
Chenopodium foliosum Asch.; Amaranthaceae; SWAT005510PilimarachFruitRaw snack ***Throat infection*Juice is extracted from ripe and clean fruits and applied to the eye to treat infections. The ripe fruits are eaten raw for their taste; they are also used for eye infections [36].-
Chenopodium murale L.; Amaranthaceae; SWAT000776Dar konakhLeavesCooked ***Constipation ***Especially used for abdominal pain, as a diuretic, and it is considered an anthelmintic [36].-
Cirsium arvense (L.) Scop.; Asteraceae; SWAT000728ChamcheerRootsRaw snack ***---
Convolvulus arvensis L.; Convolvulaceae; SWAT005968; SWAT005966MishkLeavesCooked ***Leaves are cooked as vegetables and used to relieve constipation *.Young leaves are used as vegetables to treat constipation [34]. The roots are dried, powdered, and used as a purgative, i.e., for evacuation of the bowels [36].In vitro: antioxidant [53].
Cotoneaster nummularius Fisch. & C.A.Mey.; Rosaceae;
SWAT005485		MikeenFruitRaw snack ***Increase blood ***The edible fruits are a blood
purifier [34].		-
Crataegus songarica K. Koch; Rosaceae;
SWAT005473		GoniFruitRaw snack ***Heart problems ***, increase blood ***Fruits are edible and considered a cardio tonic [36].In vivo: antioxidant effect and anticancer activity as it protects heart cells [54].
In vivo: hypotensive agent [55].
Cucurbita pepo L.; Cucurbitaceae; SWAT005999KadoFlowersCooked *Cooling agent *Vegetable is used to lower
blood pressure [34].		-
Descurainia sophia (L.) Webb ex Prantl;
Brassicaceae;
SWAT005793, SWAT005513		Kheli kheliLeavesCooked *Brain tonic **Young shoots and seeds are powdered and used for gas trouble and intestinal disorders. The decoction is used as apainkiller. Freshly collected leaves are consumed with milk for reducing high fever [36].In vitro: anti-inflammatory, analgesic, and antipyretic effects, and antioxidant and anthelmintic activities [56].
Elaeagnus angustifolia L.; Elaeagnaceae; SWAT005806,
SWAT005808		SinjurFruitRaw snack ***Respiratory problems ***Ripe fruits are boiled in water, sugar is added to enhance flavor, and a syrup is prepared. This syrup is used for sore throat and high fever [36]. Fruits are dried and powdered to treat asthma and cough. The gummy stem and branch resin is dried, then powdered, and used as a tonic shampoo for long, healthy, and silky hair [34].-
Elaeagnus rhamnoides (L.) A.Nelson; Elaeagnaceae: SWAT005998MirghinzFruitRaw snack *Blood pressure *, asthma *, gastric problems *. The fruit is also used to treat abdominal pain **.The juice obtained from the berries, called Buringogh, is used against high blood pressure and eye diseases. Berries are also applied to the face as a face mask to treat sunburn [34].In vitro: cardiovascular and cerebrovascular protection, anti-tumor, anti-inflammatory, and anti-oxidation [57].
In vitro: antifungal, anti-psoriasis, anti-atopic dermatitis, and wound healing activities [58].
In vitro and in vivo: antioxidative and immunomodulating [59,60], In vivo: cardioprotective and antiatherogenic [60,61,62],
In vitro: antibacterial and antiviral effects [63,64],
In vitro and in vivo: healing effect on acute and chronic wounds [65,66,67],
In vitro: antiradiation [68,69],
In vitro: anti-inflammatory [70],
In vitro: antidiabetic [71],
In vitro and in vivo: anticarcinogenic [59,60],
In vivo: hepatoprotective, and dermatological effects [72,73].
Ephedra intermedia Schrenk & C.A.Mey.; Ephedraceae; SWAT000772SomaniFruitRaw snack *The young shoots are ground and applied topically on the skin to treat stings and bites **.Whole plant is boiled in water, crushed, and an aqueous extract is obtained, called Gholja in the Khowar language, which is used to treat facial sunburn, pneumonia, and gastric problems [34]. Ripe fruits are boiled in water and used for asthma and tuberculosis [36].-
Eremurus stenophyllus (Boiss. & Buhse) Baker; Xanthorrhoeaceae; SWAT005967Taikh shakhLeavesCooked ***Improve digestion **--
Fallopia dumetorum (L.) Holub; Polygonaceae; SWAT005967, SWAT000774PindormishkLeavesCooked ***Healthy food **Leaves are eaten to increase appetite, and used as a purgative, astringent, and diuretic [36].-
Ferula hindukushensis Kitam.; Apiaceae; SWAT005983RawuStem, latexSeasoning ***,
pickle *** (along with vinegar)		Blood pressure *** and diabetes ***. The dried latex of the plant, locally known as Hing, is fastened around a child’s neck to prevent bad eyesight *. It is also used for piles ***. It is also used in some dishes to relieve flatulence and improve digestion ***.Locally this species is used for cough, asthma, toothache, gastric problems, and constipation [36]. Young stems are cut resulting in the oozing out of a milky exudate. It is locally called Hing, which is used to treat stomachache, diabetes, and toothache [34].-
Foeniculum vulgare Mill.;
Apiaceae; SWAT006000		BodioungSeeds/FruitsSeasoning ***Tea: chest cough **Seeds and fresh leaves are chewed for cough, abdominal pain, and pneumonia [34].-
Juglans regia L.; Juglandaceae; SWAT006042BirmoughBarkTea **Healthy food *Bark and leaves are used for gum and tooth diseases and for brightening teeth. A decoction of leaves is given for eczema. Seeds can be eaten to lower blood pressure [34].In vivo: active against cardiovascular diseases [74].
Juniperus excelsa M.Bieb.; Cupressaceae;
SWAT005497, SWAT005498		SarwoozBarkTea **The ground fruit is given to children as a vermicide ***.-In vitro: antiparasitic, nematicidal [75].
Lactuca serriola L.; Asteraceae; SWAT005965Chir jashoStemRaw snack *Healthy food *--
Lepyrodiclis holosteoides (C.A. Mey.)
Fenzl ex Fisch. and C.A. Mey.;
Caryophyllaceae; SWAT000747		BirghalAerial partsCooked **Laxative ***--
Malus domestica Borkh.; Rosaceae; SWAT006043 PaloughLeavesCooked *Healthy food **--
Malva neglecta Wallr.; Malvaceae; SWAT006043Suwachal
Shakh		LeavesCooked ***Constipation ***Leaves are used as vegetables to treat constipation and other digestive problems, and they also act as a cooling agent [34].In vitro: antioxidant potential [76].
Medicago sativa L.; Fabaceae; SWAT005797, SWAT005795MoshishLeavesCooked ***Cooling agent *, appetizer *-In vitro: antioxidant effects [77].
Mentha longifolia (L.) L.; Lamiaceae;
SWAT005792, SWAT005790		BenLeavesSalad ***Gastric problems ***.
Root: vomiting **		Gastric problems.
Herbal tea, made from the roots, called Benough, is used to cure fever, jaundice, and indigestion. The fresh and dried leaves are also eaten as a digestive and as a remedy for stomachache [34].		In vitro: antioxidant and antiemetic properties [78].
Morchella esculenta (L.) Pers.; Morchellaceae;
SWAT004746		KotchiAerial partsCooked ***The extract is useful for treating eye problems *.Useful for removing phlegm [33].In vivo: anti-inflammatory effect [79].
Nasturtium officinale R.Br.; Brassicaceae; SWAT005482Shiako shakhLeavesCooked ***Appetizer *, cooling agent ***, diuretic **Respiratory problems [33]. Leaves serve as vegetables, which are used against dyspepsia and hepatitis [34].In vitro: antioxidant activity [80].
Plantago lanceolata L.; Plantaginaceae; SWAT005962Boeeko liginiLeavesCooked **Constipation ***--
Portulaca quadrifida L.; Portulacaceae;
SWAT005970		PichiliLeavesCooked ***Constipation ***Joint pain [33].-
Rheum ribes L.; Polygonaceae; SWAT004749IshparLeaf stalksRaw snack ***
Fermentation **		Appetizer *** and sexual tonic ***Young stems and shoots are edible and eaten raw, which is used for treating flu and cough [34]. The root is used as a purgative. Leaf stalks are cooked as a vegetable. The unripe stem and leaf stalks are also eaten raw for their taste [36].-
Rosa webbiana Wall. ex Royle; Rosaceae;
SWAT005502		ThroniFruit,
Leaves		Fruit: raw snack **
Leaves: tea ***		Gastric problems **A decoction is prepared from fruits boiled in water and then strained overnight to treat asthma [36]. The petals of these plants are collected, dried, crushed, and powdered; one teaspoon of this powder is added to tea to treat stomachache [34].-
Rubus fruticosus G.N. Jones; Rosaceae; SWAT006044AtchuFruitRaw snack ***Increase blood ***Fruits are carminative and also used for diarrhea and intestinal looseness [34].In vivo: antioxidant, anti-inflammatory, and gastroprotective properties [81].
In vitro: increase plasma catalase level [82].
Rumex dentatus L.; Polygonaceae;
SWAT005468		SirkonzurLeavesCooked ***Hepatitis *, increases blood **Leaves are eaten to increase appetite; also used as a purgative, astringent, and diuretic [36]. The fresh leaves are collected, boiled, cut, and the resulting paste is mixed with tomato, onion, ginger, garlic, and salt (as required), and then fried in oil; this gravy is used as a laxative when eaten as a vegetable [34].-
Silene conoidea L.; Caryophyllaceae;
SWAT005481, SWAT005514		HapuparAerial partsCooked ***Paste from the aerial parts is made, which is used by girls to beautify their faces ***.A paste is prepared from dried young leaves and seeds and then applied on pimples; also used to relieve backache [34]. A paste is prepared by grinding seeds and young leaves, which is then applied on pimples. This paste is also used for treating backache [36].-
Solanum americanum Mill.; Solanaceae;
SWAT005503, SWAT005803		PilmilikFruitRaw snack **The berries are used by girls to heal sunburns ***.Ripened fruits are collected to extract their juice, which is effective against eye irritation and sunburn. The fruits are eaten to alleviate stomachache [34].In vivo: anti-inflammatory effect [83], anti-ulcerogenic and ulcer healing properties [84].
Taraxacum campylodes G.E.Haglund; Asteraceae; SWAT005972PawuLeavesCooked ***Constipation ***Leaves and young shoots are served as vegetables to treat constipation, as well as liver and kidney disorders [34].In vivo: hepatoprotective, anti-inflammatory, hypolipidemic, and hypoglycemic activities [85].
In vivo: antioxidant property preventing kidney damage [86].
In vivo: gastric emptying and smooth muscle motility [87].
Thymus linearis Benth.;
Lamiaceae;
SWAT000740		SewAerial partsTea ***Gastric problems ***Tea is used for stomach disorders. It is also considered a carminative and tonic [36].-
***: quoted by more than 50% of informants; **: quoted by between 50% and 25% of informants; *: quoted by less than 25% of informants.
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Abdul Aziz, M.; Ullah, Z.; Adnan, M.; Sõukand, R.; Pieroni, A. The Fading Wild Plant Food–Medicines in Upper Chitral, NW Pakistan. Foods 2021, 10, 2494. https://doi.org/10.3390/foods10102494

AMA Style

Abdul Aziz M, Ullah Z, Adnan M, Sõukand R, Pieroni A. The Fading Wild Plant Food–Medicines in Upper Chitral, NW Pakistan. Foods. 2021; 10(10):2494. https://doi.org/10.3390/foods10102494

Chicago/Turabian Style

Abdul Aziz, Muhammad, Zahid Ullah, Muhammad Adnan, Renata Sõukand, and Andrea Pieroni. 2021. "The Fading Wild Plant Food–Medicines in Upper Chitral, NW Pakistan" Foods 10, no. 10: 2494. https://doi.org/10.3390/foods10102494

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