Metabolomics Based on 1H-NMR Reveal the Regulatory Mechanisms of Dietary Methionine Restriction on Splenic Metabolic Dysfunction in Obese Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Experiment
2.2. Sample Collection
2.3. Analysis of Blood Glucose, Plasma and Spleen Lipid Parameters, and Spleen Crude Protein
2.4. NMR Spectroscopy and Data Processing
2.5. Determination of Oxidative Stress Parameters
2.6. Measurement of Inflammatory Cytokines and Immune Parameters
2.7. Assays for Spleen Glycogen, ATP/ADP/AMP, Mitochondrial DNA Copy Number, and the Respiratory Chain Enzyme Activities
2.8. Gene Expression Assays
2.9. Western Blot Analysis
2.10. Statistical Analysis
3. Results
3.1. MR Normalized Obesity-Induced Changes of Body Weight and Body Composition
3.2. MR Normalized Obesity-Induced Increases in Spleen Weight, Spleen and Plasma Lipid Levels
3.3. MR Normalized Obesity-Induced Changes in the Levels of Spleen Metabolites
3.4. MR Counteracted Obesity-Induced Inhibition in Spleen Energy Production
3.5. MR Reversed Obesity-Induced Enhancement in Spleen Oxidative Stress
3.6. MR Abolished Obesity-Induced Elevation in Spleen Inflammation
3.7. MR Normalized Obesity-Induced Decline in Immune Function
3.8. MR Abolished Obesity-Induced Inhibition in Spleen Autophagy Pathway
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Indicators | CON | DIO | DIO + MR |
---|---|---|---|
Spleen | |||
T-AOC (U/mg protein) | 0.86 ± 0.05 | 0.49 ± 0.03 * | 0.83 ± 0.06 # |
GSH-Px (U/mg protein) | 70.13 ± 2.96 | 51.64 ± 2.33 * | 73.20 ± 3.44 # |
GSH/GSSG | 1.25 ± 0.06 | 0.89 ± 0.02 * | 1.30 ± 0.05 # |
ROS (103 cd/mg) | 35.04 ± 1.67 | 57.32 ± 2.45 # | 38.36 ± 2.02 * |
MDA (nmol/mg protein) | 2.24 ± 0.12 | 3.16 ± 0.10 * | 2.18 ± 0.11 # |
Plasma | |||
T-AOC (U/mL) | 3.76 ± 0.17 | 2.57 ± 0.11 * | 3.82 ± 0.20 # |
GSH-Px (U/mL) | 73.51 ± 3.49 | 51.73 ± 2.85 * | 75.69 ± 4.02 # |
GSH/GSSG | 0.32 ± 0.02 | 0.25 ± 0.01 * | 0.34 ± 0.02 # |
ROS (103 cd/μL) | 31.26 ± 2.03 | 46.89 ± 2.69 * | 29.77 ± 2.30 # |
MDA (nmol/mL) | 13.06 ± 0.81 | 19.22 ± 0.73 * | 14.11 ± 0.71 # |
Indicators | CON | DIO | DIO + MR |
---|---|---|---|
Spleen | |||
IL-10 (pg/mg protein) | 52.03 ± 2.11 | 34.04 ± 1.55 * | 54.73 ± 2.68 # |
IL-6 (pg/mg protein) | 32.25 ± 1.30 | 39.12 ± 1.46 * | 33.45 ± 1.19 # |
IL-1β (pg/mg protein) | 46.81 ± 2.12 | 57.69 ± 1.89 * | 48.33 ± 1.75 # |
TNF-α (pg/mg protein) | 63.12 ± 2.70 | 84.56 ± 3.23 * | 66.72 ± 2.16 # |
MCP-1 (pg/mg protein) | 24.66 ± 1.08 | 32.98 ± 1.24 * | 22.21 ± 1.00 # |
Plasma | |||
IL-10 (pg/mL) | 145.60 ± 6.52 | 117.14 ± 5.07 * | 138.32 ± 5.86 # |
IL-6 (pg/mL) | 58.62 ± 1.83 | 73.09 ± 2.35 * | 53.71 ± 1.77 # |
IL-1β (pg/mL) | 67.53 ± 2.16 | 91.80 ± 2.54 * | 69.64 ± 2.09 # |
TNF-α (pg/mL) | 116.24 ± 4.05 | 150.67 ± 5.46 * | 121.31 ± 3.13 # |
MCP-1 (pg/mL) | 36.44 ± 1.20 | 49.79 ± 1.94 * | 37.08 ± 1.69 # |
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Yang, Y.; Qian, J.; Li, B.; Lu, M.; Le, G.; Xie, Y. Metabolomics Based on 1H-NMR Reveal the Regulatory Mechanisms of Dietary Methionine Restriction on Splenic Metabolic Dysfunction in Obese Mice. Foods 2021, 10, 2439. https://doi.org/10.3390/foods10102439
Yang Y, Qian J, Li B, Lu M, Le G, Xie Y. Metabolomics Based on 1H-NMR Reveal the Regulatory Mechanisms of Dietary Methionine Restriction on Splenic Metabolic Dysfunction in Obese Mice. Foods. 2021; 10(10):2439. https://doi.org/10.3390/foods10102439
Chicago/Turabian StyleYang, Yuhui, Jing Qian, Bowen Li, Manman Lu, Guowei Le, and Yanli Xie. 2021. "Metabolomics Based on 1H-NMR Reveal the Regulatory Mechanisms of Dietary Methionine Restriction on Splenic Metabolic Dysfunction in Obese Mice" Foods 10, no. 10: 2439. https://doi.org/10.3390/foods10102439