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Review

Recent Trends in Photoacoustic Imaging Techniques for 2D Nanomaterial-Based Phototherapy

by
Woo Yeup Jeong
1,†,
Moon Sung Kang
2,†,
Haeni Lee
2,
Jong Hun Lee
3,
Jeesu Kim
2,*,
Dong-Wook Han
2,* and
Ki Su Kim
1,*
1
School of Chemical Engineering, College of Engineering, Pusan National University, Busan 46241, Korea
2
Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, Korea
3
Department of Food Science and Biotechnology, Gachon University, Seongnam, Gyeonggi 13120, Korea
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomedicines 2021, 9(1), 80; https://doi.org/10.3390/biomedicines9010080
Submission received: 24 November 2020 / Revised: 13 January 2021 / Accepted: 13 January 2021 / Published: 15 January 2021
(This article belongs to the Special Issue Advanced Nanomedicines for Optical Imaging and Phototherapy)
Browse Figures
Versions Notes

Abstract

:
A variety of 2D materials have been developed for therapeutic biomedical studies. Because of their excellent physicochemical properties, 2D materials can be used as carriers for delivering therapeutic agents into a lesion, leading to phototherapy. Various optical imaging techniques have been used for the monitoring of the treatment process. Among these, photoacoustic imaging has unique advantages including relatively deep imaging depth and large field of view with high spatial resolution. In this review article, we summarize the types of photoacoustic imaging systems used for phototherapy monitoring, then we explore contrast-enhanced photoacoustic images using 2D materials. Finally, photoacoustic image-guided phototherapies are discussed. We conclude that 2D material-based phototherapy can be efficiently monitored by photoacoustic imaging techniques.

Graphical Abstract

1. Introduction

Two-dimensional nanomaterials with a well-ordered 2D planar structure (thickness > 100 nm) have been widely developed. Thanks to their beneficial biocompatible and biodegradable characteristics, various types of 2D nanomaterials, such as graphene derivatives; LDH, layered double hydroxide; TMD, transition metal dichalcogenide; TMO, transition metal oxide; and BP, black phosphorus, have been used for biomedical applications including drug delivery, tissue engineering, bio-imaging, and bio-sensing [1] (Figure 1). Because such materials have beneficial physicochemical properties of biocompatibility and degradability, they are suitable for biomedical applications including drug delivery, tissue engineering, bio-imaging, and biosensors [2,3,4,5,6]. Two-dimensional nanomaterials can cause a photothermal effect that generates heat by converting light energy into thermal energy when they are irradiated with near-infrared (NIR) light, and can then be used for phototherapy [7].
The representative phototherapy methods are photothermal therapy (PTT) and photodynamic therapy (PDT) [8]. In PTT, the local heating of NIR-absorbing agents is triggered by NIR light illumination. As tumor cells have difficulty dissipating heat, the NIR-triggered photothermal effect causes selective death of cancerous cells, which can be ablated more than 42 °C through necroptosis and apoptosis, which is programmed cell death, while endowing little damage to normal cells [9]. In contrast, PDT is an indirect method using photosensitizers that generate harmful singlet oxygen (1O2) when they absorb light. As PDT does not generate heat, nanomaterials in PDT usually perform as carriers that transfer photosensitizers using their surface properties [10]. To assess the therapy, drug delivery, and biodegradability, visualization of the internal biodistribution is essential.
Various biomedical imaging techniques, such as X-ray computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography (PET), have been used for visualizing the distribution of nanomaterials, monitoring the delivery of nanomaterials, and assessing the efficacy of treatments [11,12,13,14]. Particularly, optical imaging techniques have been widely used as they provide high optical contrast, rich functional information, and excellent spatiotemporal resolution [15]. Compared with other biomedical imaging techniques, optical imaging systems can be implemented with low cost and simple configuration. In addition, optical imaging does not create harmful ionizing radiation, which makes the system favorable for future clinical translation. However, despite the advantages set out above, optical imaging is not widely used in clinics. The primary limitation of optical imaging is shallow imaging depth due to photon scattering in biological tissue [16].
Photoacoustic (PA) imaging is a biomedical imaging technique that combines the principles of ultrasound (US) and optical imaging [17]. The principle of PA imaging is based on the PA effect, which involves energy transduction from laser to acoustic waves through thermoelastic expansion (Figure 2). The typical procedure of PA imaging is as follows: (1) illumination of a short (typically a few nanoseconds) pulsed laser beam to target tissue, (2) light absorption and heat release by the optically absorbing chromophores, (3) acoustic wave (i.e., PA wave) generation through rapid thermal expansion and relaxation, (4) signal reception using US transducer, and (5) image generation and display. Because PA imaging inherits the principles of optical and US imaging techniques, it can provide both strong optical contrast and high ultrasound resolution in deep tissue [18,19]. In addition to intrinsic chromophores (oxy- and de-oxy-hemoglobins, melanin, lipids, and water), external agents (organic dyes, liposomal nanoformulations, nanoparticles, and nanostructures) have been widely used to obtain contrast-enhanced PA images [20,21,22,23]. Moreover, using multiple wavelengths of the excitation laser, molecular functional information of biological tissue can be obtained, which can be used for investigating the bio-distribution of external agents in vivo [24,25,26].
In this review article, we summarize several types of preclinical PA imaging systems for monitoring the distribution of nanomaterials in vivo. We also introduce recent trends and therapeutic applications of PA imaging for 2D material-based phototherapy. By monitoring the delivery and theranostic procedure, we can determine the future direction of PA imaging as an investigational tool for 2D nanomaterials.

2. Photoacoustic Imaging System

In general, PA imaging systems can be classified as PA microscopy (PAM) and PA computed tomography (PACT). The main difference between the two types of system is mathematical computation in the image reconstruction algorithm. In PAM, a single-element transducer is used to achieve PA signals along the depth direction (i.e., A-line signal) at the top of the sample, thus no mathematical reconstruction algorithm is required. In contrast, PACT utilizes rotational scanning methods or multi-element US transducers to acquire tomographic information regarding the target. Therefore, PACT requires image reconstruction algorithms [28,29,30]. Compared with PACT, PAM systems have higher resolutions, but suffer from shallow imaging depth and slow imaging speed.

2.1. Photoacoustic Microscopy

PAM can be divided into two types: optical-resolution PAM (OR-PAM) and acoustic-resolution PAM (AR-PAM). OR-PAM uses tight optical focus, ~10 times smaller than acoustic focus, while the emphasis of AR-PAM is to tighten the acoustic focus. In OR-PAM, the lateral resolution is determined by the optical focal spot size, which depends on the numerical aperture of an objective lens (Figure 3A). The tightly focused optical beam excites optically absorbing chromophores only in the focal zone (red dot in Figure 3A), and the PA waves are generated from the excited chromophores. Because the optical focal spot is smaller than the acoustic focal spot, all the generated PA waves are detected by the US transducer (black arrow in Figure 3A). We can note that PA waves are not generated from the unexcited chromophores (orange dots in Figure 3A), which leads to the better resolution of OR-PAM compared with AR-PAM. In contrast, the resolution of AR-PAM is determined by the acoustic focal size because the optical focal spot is larger than the acoustic focal spot (Figure 3B). The size of the acoustic focal spot depends on the characteristics of the US transducer. The widely delivered optical beam excites chromophores in a large area (red dots in Figure 3B), then generates PA waves (black and gray arrows in Figure 3B). Among them, the PA waves in the acoustic focal zone (black arrows in Figure 3B) are detected by the ultrasound transducer. Therefore, the acoustic focus determines the resolution of the system.
By adjusting the optical focus, the resolution of OR-PAM can be greatly improved (Figure 3C) [31,32,33,34,35]. However, imaging depth can be limited because maintaining tight optical focus is difficult while scanning the imaging area. Therefore, compared with AR-PAM, OR-PAM systems usually have a small field of view (FOV) and a shallow imaging depth. As a tight optical focus is not required, AR-PAM has the advantage of being able to image deeper and larger areas in biological tissues compared with OR-PAM (Figure 3D) [36,37,38,39,40,41].
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Figure 3. Schematic illustration and representative images of OR- and AR-PAM systems. The typical configurations of (A) OR-PAM and (B) AR-PAM. The optical focus is smaller than the acoustic focus in OR-PAM, while the optical focus is larger than the acoustic focus in AR-PAM. The red and orange dots are excited and unexcited chromophores, respectively. The black and gray arrows are detected and undetected PA waves, respectively. (C) PA images of mouse ear acquired using an OR-PAM. (D) PA images of the whole body of mouse acquired using an AR-PAM. OR-PAM, optical resolution photoacoustic microscope; AR-PAM, acoustic resolution photoacoustic microscope; PA, photoacoustic; TR, ultrasound transducer; WT, water tank; OL, objective lens; SCL, spherical conical lens. The images are reproduced with permission from [31,39].
Figure 3. Schematic illustration and representative images of OR- and AR-PAM systems. The typical configurations of (A) OR-PAM and (B) AR-PAM. The optical focus is smaller than the acoustic focus in OR-PAM, while the optical focus is larger than the acoustic focus in AR-PAM. The red and orange dots are excited and unexcited chromophores, respectively. The black and gray arrows are detected and undetected PA waves, respectively. (C) PA images of mouse ear acquired using an OR-PAM. (D) PA images of the whole body of mouse acquired using an AR-PAM. OR-PAM, optical resolution photoacoustic microscope; AR-PAM, acoustic resolution photoacoustic microscope; PA, photoacoustic; TR, ultrasound transducer; WT, water tank; OL, objective lens; SCL, spherical conical lens. The images are reproduced with permission from [31,39].
Biomedicines 09 00080 g003

2.2. Photoacoustic Computed Tomography

For further enhancement of FOV and imaging speed, PA computed tomography (PACT) using array transducers has been introduced (Figure 4) [42,43,44,45]. Using multi-element transducers with various geometrical structures (linear, arc, planar, or hemispherical arrays), PACT can achieve PA signals at multiple positions with a single laser shot; therefore, the imaging speed and FOV can be significantly enhanced [45,46,47,48,49]. For potential clinical applications, clinical US machines have been used for PACT. As the receiving signals of both PA and US are acoustic waves, the signal acquisition and image reconstruction procedures of the two imaging techniques are identical, and they can thus share a single imaging platform [27,50,51,52]. Combined PA and US images, providing complementary structural and functional information regarding biological tissues, have been investigated in clinical applications such as melanoma detection, cancer classification, and vascular disease assessment [53,54,55,56].

2.3. Performance Benchmarks of Photoacoustic Imaging Systems

As each type of system has complementary advantages in terms of imaging depth, spatial resolution, imaging speed, and FOV, a variety of PA imaging systems have been applied in biomedical studies including the monitoring of phototherapy. The typical performances of PA systems are summarized in Table 1. OR-PAM has an excellent spatial resolution in a shallow depth of biological tissue, thus it is usually utilized for visualization of superficial regions such as the ear, eye, or peripheral vasculature. AR-PAM can obtain a signal that penetrates deeper into biological tissue and an extended FOV compared with OR-PAM, as is used for monitoring the whole-body distribution of nanomaterials, drugs, or contrast agents. PACT further extends FOV and imaging speed, thus it can monitor the real-time response of biological tissue during treatment using nanomaterials.

3. Phototherapy Using 2D Nanomaterials

3.1. Types and Characteristics

Currently, various phototherapy agents including gold nanostructures [57,58], mesoporous silica nanoparticles [59,60], and 2D nanomaterials have been widely investigated for clinical application. Among them, 2D nanomaterials are attracting the most attention because they can be used not only for phototherapy, but in other biomedical fields thanks to their excellent biochemical properties (Table 2). Typical 2D nanomaterials consist of one or a few atomic layers. As a representative 2D nanomaterial, graphene has a honeycomb structure consisting of carbon atoms. By changing the shape, the number of layers, and chemical modifications, several derivatives can be formed. Among them, graphene oxide (GO) [57] and reduced graphene oxide (rGO) [58,59] have been widely utilized for biomedical applications because of their superior electrical and thermal conductivities, large surface area, chemical versatility, and biocompatibility [60,61,62]. Layered double hydroxides (LDHs) are inorganic 2D nanomaterials with structures in which metal atoms are sandwiched by hydroxide layers. Because of their high charge density with excellent biocompatibility, LDHs are widely used as nano-carriers for theranostic agents in drug or gene delivery, phototherapy, and immunotherapy [63,64,65]. Transition metal dichalcogenides (TMDs) have hexagonal lattices consisting of monolayers of transition metal atoms between chalcogen atom layers. In addition to thin, flexible, and strong characteristics, TMDs are excellent optical absorbers, thus producing photolimunescence and photothermal effects [66,67,68,69]. TMDs can be formed with various materials including molybdenum disulfide (MoS2) [70,71,72,73], tungsten disulfide (WS2) [74,75,76,77], and molybdenum diselenide (MoSe2) [78,79,80]. Transition metal oxides (TMOs) are compounds of oxygen atoms and transition metals such as titanium (TiO2) [81,82] and manganese (MnO2) [83,84,85]. Their wide bandgap results in excellent photochemical and electric properties [86,87]. They can also directly interact with drugs, genes, or other biomolecules by surface modification and thus can be utilized in biomedical applications including drug delivery, cancer therapy, tissue engineering, bio-imaging, and biosensing [88,89,90]. Mxenes are the most recently discovered 2D materials and have been applied in various biomedical applications because of their extreme thinness, large surface area, high surface–volume ratio, and mechanical strength [91,92,93,94,95]. Black phosphoruses (BP) are the most stable allotropes of phosphorus with zigzag or armchair bilayer structures. BP have shown potential in biomedical applications with their strong optical absorption in the ultraviolet (UV) and NIR regions [96,97,98,99,100]. They have also demonstrated promising biocompatibility and biodegradation [101].

3.2. Phototherapy Using 2D Nanomaterials

By taking advantage of their strong optical absorption and thermal transition properties, 2D nanomaterials have been used for PTT. Liu et al. successfully prepared a doxorubicin (DOX)-loaded MoS2-PEG nanosheet for combined PTT–chemo cancer therapy [102]. In that study, nanosheets were analyzed for drug delivery and photothermal effects by NIR irradiation. MoS2-PEG/DOX nanosheets exhibited synergistic anti-cancer effects, inhibiting tumor growth in in vivo experiments. Zeng et al. reported on ultrathin MnO2 nanosheets of polyethylene glycol-cyclic arginine-glycineaspartic acid tripeptide (PEG-cRGD) and encapsulated chlorin e6 (Ce6) for PTT/PDT synergistic cancer therapy (Figure 5) [103]. In that study, the nanosheets showed photothermal efficiency of 39% and could be reduced by overexpressed acidic H2O2, which could efficiently generate O2 and further enhance the therapeutic efficiency of PDT. Moreover, the MnO2-PEG-cRGD/Ce6 exhibited pH-controlled and NIR-induced Ce6 release, and showed favorable therapeutic outcomes under a single 660 nm NIR laser.
A number of PDT studies have investigated the development of 2D nanomaterials that release photosensitizers. Moosavi et al. prepared N-TiO2 nanoparticles to generate reactive oxygen species (ROS) and induce autophagy [81]. They showed the dose-dependent capability of well-dispersed photo-activated N-TiO2 NPs to induce terminal megakaryocyte differentiation and cell death in K562 leukemia cells. In cellular experiments, N-TiO2 nanoparticles increased ROS levels with light irradiation. Yang et al. fabricated covalently, incorporating both chlorin e6 (Ce6) and triphenyl phosphonium (TPP) onto BP@PDA NSs for dual-modal imaging-guided synergistic photothermal and photodynamic therapy (Figure 6) [104]. BP@PDA–Ce6&TPP NSs can produce considerable heat, mainly owing to BP@PDA NSs, and can generate sufficient ROS for PDT. With these results, BP@PDA–Ce6&TPP NSs can be used for PTT/PDT therapy of cancers.
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Figure 6. (A) Schematic illustrations of the preparation, therapeutic uses, and peaks in functions of BP@PDA–Ce6&TPP NSs. (B) Ex vivo fluorescence imaging of main organs as well as tumor at 24 h post-injection. (C) Relative viabilities of HeLa cells incubated with BP@PDA NSs, BP@PDA–Ce6 NSs, and BP@PDA–Ce6&TPP NSs at different BP@PDA concentrations with laser illumination (660 nm, 0.5 W cm−2, 5 min). Data represent mean ± SD (n = 4). The images are reproduced with permission from [104].
Figure 6. (A) Schematic illustrations of the preparation, therapeutic uses, and peaks in functions of BP@PDA–Ce6&TPP NSs. (B) Ex vivo fluorescence imaging of main organs as well as tumor at 24 h post-injection. (C) Relative viabilities of HeLa cells incubated with BP@PDA NSs, BP@PDA–Ce6 NSs, and BP@PDA–Ce6&TPP NSs at different BP@PDA concentrations with laser illumination (660 nm, 0.5 W cm−2, 5 min). Data represent mean ± SD (n = 4). The images are reproduced with permission from [104].
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Table
Table 2. Phototherapy using 2D nanomaterials. PTT, photothermal therapy; PDT, photodynamic therapy.
Table 2. Phototherapy using 2D nanomaterials. PTT, photothermal therapy; PDT, photodynamic therapy.
2D NanomaterialsPhotothermal ConversionTherapyApplied FormsRef.
Graphene derivatives63% (G), 35% (GO) [59]PTT, PDTGO-UCNPs-ZnPc[61]
PTTGO/MnFe2O4/DOX[62]
TiO240.8% [86]PTTAg@TiO2[82]
PDTN-TiO2[81]
MoS20.84% [67]PTTMoS2-HA-DTPA-Gd[71]
PTT, PDTAuNBPs@MoS2[72]
PTTMoS2-Gd-BSA[73]
BP30.84% [97]PTTBP-Au NSs[98]
PTTBP-PEG-FA/Cy7 NSs[99]
PTT, PDTBP@PEG/Ce6 NSs[100]
Mxene(Ti3C2)≒100% [92]PTTTi3C2@Au[93]
PTT, PDTTi3C2-SP[94]
PTT, PDTTi3C2-DOX[95]
WS235% [68]PTT, PDTBSA-WS2@MB[75]
PTTWS2-PEG[76]
PTTWS2-IO/S@MO-PEG[77]
MoSe254.3% [69]PTT, PDTMoSe2/Fe3O4[79]
PTT, PDTMoSe2@PEG-Dox[80]
2D Boron42.5% [104]PTT, PDTB@Ce6–PAH–PAA[105]
MnO262.4% [87]PTTMnO2-PEG-FA/DOX[84]
PTTBSA-MnO2 NPs[85]

4. Photoacoustic Image-Guided Phototherapy

4.1. Contrast-Enhanced Photoacoustic Imaging Using 2D Nanomaterials

PA imaging, one of the latest and most promising imaging modalities, has been shown to enable visualization of tissues with centimeter-depth penetration for in vivo imaging (Table 3). However, the optical contrast ratio of tissues is generally low; therefore, conventional PAI can only detect specific tissues that have strong absorbance. However, certain kinds of imaging probes can be utilized to enhance the optical contrast ratio of tissues with low absorbance. Specifically, incorporating 2D NMs that strongly absorb light in the tissues to be irradiated can overcome the inherently low contrast problem. When incorporating 2D NMs exhibiting PT properties into tissues with low contrast, they absorb light more than the surrounding tissue components, hence emitting a stronger PA signal.
BP is a newly emerging 2D nanomaterial that exhibits a layer-dependent bandgap, resulting in versatile properties for electronic and optoelectronic application. However, BP is highly reactive to oxygen and water, hence its physical properties, biodistribution, and optical properties can be altered in an in vivo environment. Several studies have focused on the stabilization of BP for PA applications. Sun et al. fabricated TiL4-coordinated BPQDs (TiL4@BPQDs) for PA imaging of cancer [103]. TiL4 enhanced the stability of BPQDs in aqueous dispersions and maintained optical properties for a prolonged time compared with bare BPQDs. A PA signal was observed from the MCF-7 cells after the addition of only 12.5 ppm of TiL4@BPQDs, which is only 0.63% of the common imaging dose of 2 mg/L, suggesting their high sensitivity in in vitro PA imaging (Figure 7A). Moreover, the in vivo PA images indicated a sixfold increase in PA signal at 680 nm after injection of TiL4@BPQDs into xenografted tumors in mice (Figure 7B), which was superior to the signal generated by Au nanorods (a common PA exogenous probe). These results suggest the potential of BP for PA imaging if the unstable in vivo properties can be resolved by surface modification.
Tumor targetability is one of the most promising strategies for enhancing the PA signal in cancer imaging. The tumor microenvironment (TME) is characterized by high H2O2 and glutathione (GSH) levels, low pH value, and hypoxia; therefore, the incorporation of TME-responsive NM suggests potential for tumor-specific PAI. Yan et al. introduced Ce6-modified CoMn-LDH nanosheets for an enhanced tumor microenvironment response [106]. The CoMn-LDH nanosheets exerted complete quenching of 1O2 generation by the browning of the particle. The results indicated a good linear correlation between PA signal intensity and Ce6/CoMn-LDH concentration within 0–100 μg/mL. In vivo injection of the Ce6/CoMn-LDH nanosheets produced a weak signal that was observed after 2 h injection and increased gradually from 2 to 12 h after injection, indicating the accumulation of Ce6/CoMn-LDH nanosheets in the tumor area. The results demonstrated that Ce6/CoMn-LDH nanosheets accumulated in the tumor owing to the enhanced permeability and retention (EPR) effect, which presented as PA-combined multimodal imaging agents.
rGO, a derivative of graphene, has also been adopted as a promising PA probe thanks to its high drug loading capacity and optical absorption property in the NIR regime. Chen et al. fabricated an indocyanine green (ICG)-conjugated rGO nanocomposite for dual use of PAI and fluorescence imaging (Figure 7C) [107]. The nanocomposite was shown to exhibit little toxicity and high PA/fluorescence signals both in vitro and in vivo (Figure 7D). ICG-rGO nanocomposites showed high cytocompatibility at a concentration of 100 µg/mL and there was no obvious tissue damage in the heart, liver, spleen, kidney, or lung after injection of the particle. Moreover, ICG-rGO nanocomposites induced 63.5% of cellular uptakes and then exhibited strong red fluorescence. In vivo fluorescence imaging and PA imaging also demonstrated that ICG-rGO nanocomposites accumulated in the tumor and significantly enhanced the imaging contrasts. rGO is considered a promising candidate as a multimodal imaging probe for cancer diagnosis.

4.2. Photoacoustic Imaging for Monitoring Phototherapy

As an emerging phototheranostic modality, interest in PA-combined PTT has increased in recent tumor treatment research [76,128,129,130]. After PAI provides guidance towards the tumor site and reveals strong tumor contrast after injection, the targetability and efficiency of PTT can be significantly increased (Figure 8). Several 2D nanomaterials have multimodal characteristics with PT and PA properties; therefore, they have been extensively studied for PAI-guided PTT. AM, the 2D layered material exfoliated from bulk antimony (Sb), has been highlighted because of its superior physicochemical properties compared with typical 2D nanomaterials such as graphene, MoS2, WS2, and BP [131]. Yu et al. fabricated liquid phase exfoliated AM nanoflakes (AMNFs) exhibiting unique PA properties, making AMNFs superior to both other 2D materials, such as BP, GO, and TMDs, and commonly used PA contrast agents, such as indocyanine green (ICG) and gold nanorods (AuNRs) [108]. The AMNFs exhibited 42.36% PT conversion efficiency, which was about two times higher than other 2D NMs (e.g., GO 25%, BP 28%, MoS2 28%, AuNRs 21%). The PA performance of 1000 µg/mL AMNFs exhibited a limit of detection (LOT) of 7.4 × 104 cells, which correlates with a 0.74 mm3 tumor. It is known that about 85% of the human tumor is composed of 108 cells of 1 cm3 [132]. Therefore, it can be demonstrated that the PA performance of the AMNFs is very sensitive. Sb2Se3, a chalcogenide formed from antimony and selenium, has also emerged as a promising PA-PT multimodal agent thanks to its attractive properties such as a proper optical bandgap (1.1–1.3 eV), high light absorption coefficient, low toxicity, and abundant material reserves [133]. However, Sb2Se3 has a unique crystal structure that inherently tends to grow into a one-dimensional structure, which makes it difficult to fabricate Sb2Se3 with the desirable 2D planar nanostructure. Zhou et al. reported on the construction of Sb2Se3 nanosheet highly efficient PA-guided PTT agents by NIR laser activation on mice with 4T1 tumor cells were inoculated on their thigh (Figure 8) [109]. The Sb2Se3 nanosheets were fabricated using liquid nitrogen pretreatment and a freeze–thawing approach, resulting in a high PT-conversion capability agent (extinction coefficient: 33.2 L/g·cm; photothermal conversion efficiency: 30.78%). Moreover, the poly(vinyl pyrrolidone) (PVP) surface engineering enhanced the biocompatibility and physiological stability, which led to a prolonged and 7.3 times increased PA signal after 6 h of in vivo injection compared with that of the initial value of the tumor. AM-based 2D NMs are promising PT-PA agents, possessing great PA properties and PT-conversion efficiency.
The direct exfoliation of 2D ultrathin ceramic nanosheets from layer-structured solid and hard MAX-phase ceramics has been introduced, which has been termed MXene by Gogosti et al. [134]. The ultrathin planar structure of MXene endows the sheets with a large surface area, providing not only abundant anchoring sites for therapeutic drugs, but also high PT and PA conversion efficiency. Han et al. explored the PA and PT performances of Ti3C2 Mxene [94]. These Ti3C2 MXenes exhibited a PA signal at an excitation wavelength of 808 in vitro and a significant increase in PA signal at a concentration of 15 mg/kg (particles per 4T1-beared tumor), indicating the potential for PAI-guided therapy. Ti3C2 Mxene also showed high in vivo compatibility and easy excretion out of the body, suggesting potential high biosafety for further clinical translation. However, MXenes are limited in respect to their use as diagnostic imaging agents because of their simple material composition. Several studies have been carried out to endow them with specific functionality for theranostic application. Dai et al. fabricated SP-modified MnOx/Ta4C3-based nanosheets [111]. The PT-conversion performance of the SP-MnOx/Ta4C3 composite nanosheets showed fast enhanced PA signals (highest optical density at 15 min) after subcutaneous administration in 4T1 tumor-bearing mice. Moreover, the SP-MnOx/Ta4C3 nanosheets exhibited contrast-enhanced properties in both CT and MR, suggesting the potential use of SP-MnOx/Ta4C3 nanosheets for multiple imaging tool-guided therapy.
As the other class of 2D-layered NMs, Bi2Se3 has been highlighted as a remarkable thermoelectric and photoelectric agent possessing suitable bioactivity and biocompatibility [135,136,137]. Xie et al. fabricated PVP-encapsulated Bi2Se3 nanosheets possessing an extinction coefficient of 11.5 L/g·cm at 808 nm, showing a PT conversion efficiency of 34.6% and excellent photoacoustic performance (about a twofold increase after 5 h of injection) [112]. The PVP-Bi2Se3 nanosheets showed high cytocompatibility in vitro (about 95% of cell viable after 48 h of 200 ppm PVP-Bi2Se3 treatment) and almost perfect in vivo clearance (in the liver, spleen, kidney, lung, and heart) after 30 days of intravenous injection.
MoS2, one of the novel 2D nanomaterials, exhibits unique visible photoluminescence with high absorption in the NIR range. Despite the superior optical properties, the use of MoS2 nanosheets for in vivo theragnosis agents is hampered by their instability and low intracellular delivery efficiency [138,139]. To overcome these issues, Shin et al. introduced HA-conjugated MoS2 nanosheets to induce HA receptor-mediated endocytosis of the nanosheets [115]. HA is one of the biopolymers used for cancer cell-specific targeting because overexpressed cancer cell receptors such as cluster determinant 44 (CD44), hyaluronan receptor for endocytosis (HARE), and lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are reactive to the HA [140,141]. Therefore, HA-MoS2 conjugates experience receptor-mediated endocytosis and intracellular disulfide cleavage by pH and GSH, and subsequently, HA degrades and MoS2 nanosheets accumulate in the cells. In this method, PAI in tumor-bearing mice showed a 1860-fold increase in PA signal compared with control groups, which resulted from the high light-to-heat conversion efficiency of HA-MoS2.
Pd nanosheets, as typical 2D nanomaterials with well-defined and controllable sizes, are promising candidates in PA-guided PTT owing to their strong NIR absorption and excellent biocompatibility [142,143,144]. Zhu et al. conjugated DOX, Pd, and ZIF-8 in a self-assembled one-pot method, and coated them with PDA to improve biocompatibility (DOX/Pd@ZIF-8@PDA) [118]. The prepared DOX/Pd@ZIF-8@PDA showed a ninefold increase in PA signal after 400 ppm NMs were injected in tumor-bearing mice. Moreover, as the concentration increased from 0 to 800 ppm, the temperature rose from 25 to 54 °C after 808 nm laser irradiation, indicating a high PT conversion efficiency for DOX/Pd@ZIF-8@PDA. Interestingly, the introduction of an 808 nm laser could significantly accelerate the release of DOX from DOX/Pd@ZIF-8@PDA. Hence, DOX/Pd@ZIF-8@PDA can be adopted as multimodal theranostic agents, which is feasible for PA-guided PTT and controlled drug delivery, making them ideal candidates for a smart and multifunctional theranostic platform.

5. Conclusions and Perspectives

PA imaging has emerged as a premier preclinical biomedical imaging technique with various potential configurations. Moreover, many studies have investigated the clinical translation of PA techniques. Although there are limitations in respect to clinical translation at this time, PA imaging may be advantageous for diagnosing diseases and monitoring therapeutic prognoses. As a potential clinical monitoring system, PA images have been used for monitoring phototherapy prognoses using the delivery of 2D nanomaterials that have strong optical absorption properties. To achieve a strong optical contrast in PA images, various structures of 2D nanomaterials have been investigated and showed high optical contrast in PA images as well as a promising therapeutic effects. For future clinical translation and commercialization, 2D nanomaterials should meet requirements such as low toxicity, effective clearance, and high biodegradability. In addition, PA imaging systems need to be improved to achieve a higher signal-to-noise ratio in deep tissue. Because a laser source with a tunable wavelength is costly, 2D nanomaterials that absorb the 1064 nm wavelength (the default wavelength of Nd:YAG laser) would be a good option for successful commercialization through reducing the system cost.

Author Contributions

W.Y.J., M.S.K. and J.H.L. reviewed and summarized articles, prepared figures, and wrote the manuscript for the part on 2D nanomaterials. H.L. summarized articles and prepared figures for reviewing photoacoustic imaging systems. J.K., D.-W.H., and K.S.K. conceived the structure of the paper, supervised the review, organized the figures, and wrote the manuscript. All authors contributed to the critical reading and writing of the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research was supported by a grant from the Korea Health Technology R&D Project through KHIDI (HI18C2383), funded by the Ministry of Health & Welfare, Republic of Korea and National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2019R1A4A1024116).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

No new data were created or analyzed in this study. Data sharing is not applicable to this article.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Schematic illustration of representative 2D nanomaterials. LDH, layered double hydroxide; TMD, transition metal dichalcogenide; TMO, transition metal oxide; BP, black phosphorus.
Figure 1. Schematic illustration of representative 2D nanomaterials. LDH, layered double hydroxide; TMD, transition metal dichalcogenide; TMO, transition metal oxide; BP, black phosphorus.
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Figure 2. Schematic explanation of the procedure of PA imaging. PA, photoacoustic; US, ultrasound. The inset image is reproduced with permission from [27].
Figure 2. Schematic explanation of the procedure of PA imaging. PA, photoacoustic; US, ultrasound. The inset image is reproduced with permission from [27].
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Figure 4. Schematic illustration and representative images of a PACT system. (A) Typical configurations of PACT. Array transducer is used to acquire PA images. (B) Representative PACT images of mouse brain in vivo. PA, photoacoustic; PACT, photoacoustic computed tomography; TR, ultrasound transducer; US, ultrasound; DAQ, data acquisition module; BS, brain stem; HY, hypothalamus; ICA, internal carotid artery; LCH, left cerebral hemisphere; RCH, right cerebral hemisphere; ACA, anterior cerebral artery; ZMI, zygomatic muscle interface. The images are reproduced with permission from [42].
Figure 4. Schematic illustration and representative images of a PACT system. (A) Typical configurations of PACT. Array transducer is used to acquire PA images. (B) Representative PACT images of mouse brain in vivo. PA, photoacoustic; PACT, photoacoustic computed tomography; TR, ultrasound transducer; US, ultrasound; DAQ, data acquisition module; BS, brain stem; HY, hypothalamus; ICA, internal carotid artery; LCH, left cerebral hemisphere; RCH, right cerebral hemisphere; ACA, anterior cerebral artery; ZMI, zygomatic muscle interface. The images are reproduced with permission from [42].
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Figure 5. (A) Schematic illustration for the preparation of MnO2-PEG-cRGD/Ce6 for synergistic photothermal/photodynamic (PTT/PDT) therapy. (B) Relative viabilities of PC3 cells after incubation with free Ce6, MnO2-PEG-cRGD with 660 nm light irradiation, or MnO2-PEG-cRGD/Ce6 with or without 660 nm light irradiation (0.6 W cm−2, 10 min, *** p < 0.001, ** p < 0.01). (C) Fluorescence images of calcein acetoxymethyl ester (Calcein-AM, green)/propidium iodide (PI, red) double stained cells after different treatments. The images are reproduced with permission from [103].
Figure 5. (A) Schematic illustration for the preparation of MnO2-PEG-cRGD/Ce6 for synergistic photothermal/photodynamic (PTT/PDT) therapy. (B) Relative viabilities of PC3 cells after incubation with free Ce6, MnO2-PEG-cRGD with 660 nm light irradiation, or MnO2-PEG-cRGD/Ce6 with or without 660 nm light irradiation (0.6 W cm−2, 10 min, *** p < 0.001, ** p < 0.01). (C) Fluorescence images of calcein acetoxymethyl ester (Calcein-AM, green)/propidium iodide (PI, red) double stained cells after different treatments. The images are reproduced with permission from [103].
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Figure 7. (A) In vitro PA signals and optical absorbances of TiL4-coordinated black phosphorus quantum dots (BPQDs) with various excitation wavelengths. (B) In vivo PA images of MCF-7 tumor xenografted Balb/c mice before and after intravenous injection of TiL4-coordinated BPQDs. (C) Schematic diagram of ICG-rGO nanocomposite preparation. (D) In vitro and in vivo PA signals of ICG-rGO nanocomposites with an excitation wavelength of 780 nm. The in vivo images of HeLa tumor inoculated Balb/c nude mice were acquired before and after tail vein injection of rNGO-PEG/ICG. PA, photoacoustic; NGO, nano-graphene oxide; rNGO, reduced nano-graphene oxide; PEG, polyethylene glycolated; ICG, indocyanine green. The images are reproduced with permission from [103,107].
Figure 7. (A) In vitro PA signals and optical absorbances of TiL4-coordinated black phosphorus quantum dots (BPQDs) with various excitation wavelengths. (B) In vivo PA images of MCF-7 tumor xenografted Balb/c mice before and after intravenous injection of TiL4-coordinated BPQDs. (C) Schematic diagram of ICG-rGO nanocomposite preparation. (D) In vitro and in vivo PA signals of ICG-rGO nanocomposites with an excitation wavelength of 780 nm. The in vivo images of HeLa tumor inoculated Balb/c nude mice were acquired before and after tail vein injection of rNGO-PEG/ICG. PA, photoacoustic; NGO, nano-graphene oxide; rNGO, reduced nano-graphene oxide; PEG, polyethylene glycolated; ICG, indocyanine green. The images are reproduced with permission from [103,107].
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Figure 8. (A) Schematic diagram of exfoliation and PEGylation of WS2 nanosheets. (B) In vivo PA images of 4T1 tumor bearing Balb/c mice before, after IT injection, and after IV injection of WS2 nanosheets. (C) The growth of 4T1 tumors in different groups of mice after various treatments indicated. The relative tumor volumes were normalized to their initial sizes. (D) Schematic diagram of TiS2-PEG nanosheets. (E) In vivo PA images of 4T1 tumor bearing Balb/c mice before, 4 h after, and 12 h after IV injection of TiS2-PEG solution. (F) The growth of 4T1 tumors in different groups of mice after various treatments indicated. The relative tumor volumes were normalized to their initial sizes. PA, photoacoustic; IT, intratumoral; IV, intravenous. The images are reproduced with permission from [76,128].
Figure 8. (A) Schematic diagram of exfoliation and PEGylation of WS2 nanosheets. (B) In vivo PA images of 4T1 tumor bearing Balb/c mice before, after IT injection, and after IV injection of WS2 nanosheets. (C) The growth of 4T1 tumors in different groups of mice after various treatments indicated. The relative tumor volumes were normalized to their initial sizes. (D) Schematic diagram of TiS2-PEG nanosheets. (E) In vivo PA images of 4T1 tumor bearing Balb/c mice before, 4 h after, and 12 h after IV injection of TiS2-PEG solution. (F) The growth of 4T1 tumors in different groups of mice after various treatments indicated. The relative tumor volumes were normalized to their initial sizes. PA, photoacoustic; IT, intratumoral; IV, intravenous. The images are reproduced with permission from [76,128].
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Table
Table 1. Performance benchmarks of representative PA imaging systems. PA, photoacoustic; OR-PAM, optical resolution photoacoustic microscopy; AR-PAM, acoustic resolution photoacoustic microscopy; PACT, photoacoustic computed tomography; FOV, field of view.
Table 1. Performance benchmarks of representative PA imaging systems. PA, photoacoustic; OR-PAM, optical resolution photoacoustic microscopy; AR-PAM, acoustic resolution photoacoustic microscopy; PACT, photoacoustic computed tomography; FOV, field of view.
TypeLateral ResolutionAxial ResolutionImaging DepthImaging Time FOVRef.
OR-PAM2.56 μm-1.2 mm70 min7.8 × 10 mm2[31]
3.6 μm27.7 μm1 mm7 s9 × 4 mm2[34]
2.7 μm46.4 μm400 μm4 min6 × 8 mm2[35]
AR-PAM590 μm150 μm25 mm20 min60 × 32 mm2[39]
45 μm33 μm7.6 mm10 min9 × 7 mm2[40]
84 μm38 μm2.3 mm224 s36 × 80 mm2[41]
PACT250 μm100 μm13 mm16 s25 × 30 mm2[42]
1.5 mm-10 mm0.1 s20 × 20 mm2[44]
1.2 mm205 μm30 mm0.2 s40 × 60 mm2[27]
Table
Table 3. Recent photothermal (PT)-Photoacoustic Imaging(PAI) multimodal studies classified by their characteristics.
Table 3. Recent photothermal (PT)-Photoacoustic Imaging(PAI) multimodal studies classified by their characteristics.
PT-MultimodalityKey MaterialFormulationModification/Functionalization/HybridizationExperimentalRef.
xBlack phosphorus (BP)Quantum dotSulfonic ester of the titanium ligand (TiL4)MCF-7, 293T cells, and MCF-7 tumor-bearing Balb/c nude mice[103]
Layered double hydroxide (LDH)NanosheetCoMn and chlorin e6 (Ce6)HeLa, U87mg, HepG2, 4T1 cells, and HeLa tumor-bearing Balb/c nude mice[106]
Reduced graphene oxide (rGO)NanocompositePolyethylene glycol (PEG), indocyanine green (ICG)Hela cells, Balb/c nude mice[107]
oAntimonene (AM)Liquid phase nanoflakePEG293T, MCF-7, SK-BR3, T47D cells, and MCF-7 tumor-bearing mice[108]
Antimony (III) Selenide (Sb2Se3)NanosheetPoly(vinyl pyrrolidone) (PVP)4T1, MBA-MD-231 cells, Balb/c nude mice[109]
Tantalum carbide
(Ti3C2 MXene)		NanosheetSP (soybean phospholipid) and doxorubicin (DOX)4T1 cells, 4T1-inoculated mice[94]
Tantalum carbide (Ta4C3 Mxene)NanosheetMnO, SP4T1 cells, Kunming mouse, 4T1 tumor-bearing mice, and Balb/c nude mice[110]
MXene (Ta4C3)NanosheetManganese oxide nanoparticles (MnOx) and SP4T1 cells and 4T1 tumor-bearing nude mice[111]
Bismuth selenide
(Bi2Se3)		NanosheetNoneMCF-7 cells and MCF-7 tumor-bearing Balb/c nude mice[112]
Bi2O2SeQuantum dotNoneA549, MCF-7 cells, and MCF-7 tumor-bearing Balb/c nude mice[113]
Bi2Se3NanodishHA, polypyrrole (PPy), and zinc phthalocyanine (ZnPc)4T1 cells and 4T1 tumor-bearing Balb/c nude mice[114]
Molybdenum disulfide (MoS2)NanoconjugateHyaluronic acid (HA)HCT116 cells and HCT116-inoculated mice[115]
MoS2NanosheetIron oxide nanoparticle (IONP) and PEGBalb/c mice and 4T1 tumor-bearing mice[116]
MoS2 and Bi2S3NanosheetPEGL929 cells and 4T1 tumor-bearing Balb/c nude mice[117]
Palladium (Pd)NanosheetDOX, Zeolitic imidazolate frameworks (ZIF-8), Polydopamine (PDA)WBCs (white blood cells) from mice, 4T1 cells, and 4T1 tumor-bearing mice[118]
PdNanoplateAu and PEG4T1 tumor-bearing Balb/c mice[119]
Titanium (Ti)NanosheetPEGSMMC-7721, B16, J774A.1 cells, and Balb/c nude mice[120]
GermaneneQuantum dotPEGMCF-7, 4T1, H1299, HeLa cells, and Balb/c mice[121]
BoronNanosheetPEGHeLa, PC3, MCF-7, A549 cells, and MCF-7 tumor-bearing mice[104]
Manganese borideNanosheetBi4T1 cells and 4T1 tumor-bearing mice[122]
MnO2NanosheetNoneU87MG cells and U87MG tumor-bearing mice[123]
Tungsten disulfide (WS2)NanosheetPVPL929, HT29 cells, KM mice, and HT29 tumor-bearing KM mice[124]
Co9Se8NanoplatePoly(acrylic acid) (PAA), DOXHepG2 cells, HepG2 tumor-bearing Balb/c-nude mice[125]
AuNanoringNoneRaw 264.7 cells, U87MG tumor-bearing nude mice[126]
Cu2MnS2NanoplateMonomethoxycarboxyl polyethylene glycol (mPEG-COOH)HeLa, S180 cells, and S180 tumor-bearing Balb/c nude mice[127]
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Jeong, W.Y.; Kang, M.S.; Lee, H.; Lee, J.H.; Kim, J.; Han, D.-W.; Kim, K.S. Recent Trends in Photoacoustic Imaging Techniques for 2D Nanomaterial-Based Phototherapy. Biomedicines 2021, 9, 80. https://doi.org/10.3390/biomedicines9010080

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Jeong WY, Kang MS, Lee H, Lee JH, Kim J, Han D-W, Kim KS. Recent Trends in Photoacoustic Imaging Techniques for 2D Nanomaterial-Based Phototherapy. Biomedicines. 2021; 9(1):80. https://doi.org/10.3390/biomedicines9010080

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Jeong, Woo Yeup, Moon Sung Kang, Haeni Lee, Jong Hun Lee, Jeesu Kim, Dong-Wook Han, and Ki Su Kim. 2021. "Recent Trends in Photoacoustic Imaging Techniques for 2D Nanomaterial-Based Phototherapy" Biomedicines 9, no. 1: 80. https://doi.org/10.3390/biomedicines9010080

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