Comprehensive Analysis of Disulfidptosis-Related LncRNAs in Molecular Classification, Immune Microenvironment Characterization and Prognosis of Gastric Cancer
Abstract
:1. Introduction
2. Methods
2.1. Data Collection
2.2. Identification of Disulfidptosis-Related lncRNAs and Differential Expression Analysis in GC
2.3. The Construction of the DRLs Signature
2.4. Prognostic and Independent Analysis
2.5. Risk Score Signature-Based Establishment and Evaluation of Nomogram in GC
2.6. Functional Enrichment Analysis
2.7. Assessment of Tumor Microenvironment (TME) and Immune Cell Infiltration Levels
2.8. Tumor Somatic Mutation and Tumor Mutation Burden Analysis
2.9. The Effect of DRLs on Immunotherapy for GC
2.10. The Potential Drug Sensitivity Prediction in GC
2.11. Statistical Analysis
3. Results
3.1. Identification of Disulfidptosis-Related Differentially Expressed lncRNAs in Gastric Cancer and Construction of Risk Model
3.2. Prognostic Analysis and Internal Validation of DRLs Signature
3.3. DRLs Signature as an Independent Risk Factor in GC
3.4. The Construction and Validation of the Nomogram
3.5. Functional Enrichment Analysis of the DRLs Signature
3.6. Gene Mutation Landscape
3.7. Tumor Immune Microenvironment Landscape in High- and Low-Risk Groups
3.8. The Effect of DRLs on Immunotherapy for GC
3.9. Drug Sensitivity Analysis in DRLs Risk Model
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
AUC | Area under the curve |
BP | Biological processes |
CI | Confidence intervals |
CC | Cellular components |
DRLs | Disulfidptosis-related lncRNAs |
GC | Gastric cancer |
GSEA | Gene Set Enrichment Analysis |
GO | Gene Ontology |
lncRNAs | Long Non-coding RNAs |
KEGG | Kyoto Encyclopedia of Genes and Genomes |
K–M | Kaplan–Meier |
LASSO | Least Absolute Shrinkage and Selection Operator |
MF | Molecular functions |
OS | Overall Survival |
PFS | Progression Free Survival |
ROC | Receiver operating characteristic |
ssGSEA | Single-sample Gene Set Enrichment Analysis |
TME | Tumor Microenvironment |
TCGA | The cancer Genome Atlas |
TMB | Tumor Mutation Burden |
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Kang, K.; Li, X.; Peng, Y.; Zhou, Y. Comprehensive Analysis of Disulfidptosis-Related LncRNAs in Molecular Classification, Immune Microenvironment Characterization and Prognosis of Gastric Cancer. Biomedicines 2023, 11, 3165. https://doi.org/10.3390/biomedicines11123165
Kang K, Li X, Peng Y, Zhou Y. Comprehensive Analysis of Disulfidptosis-Related LncRNAs in Molecular Classification, Immune Microenvironment Characterization and Prognosis of Gastric Cancer. Biomedicines. 2023; 11(12):3165. https://doi.org/10.3390/biomedicines11123165
Chicago/Turabian StyleKang, Kuo, Xuanxuan Li, Yuanhao Peng, and Yangying Zhou. 2023. "Comprehensive Analysis of Disulfidptosis-Related LncRNAs in Molecular Classification, Immune Microenvironment Characterization and Prognosis of Gastric Cancer" Biomedicines 11, no. 12: 3165. https://doi.org/10.3390/biomedicines11123165