Russet Susceptibility in Apple Is Associated with Skin Cells that Are Larger, More Variable in Size, and of Reduced Fracture Strain
Abstract
:1. Introduction
2. Results
2.1. Morphology of the Skin of Developing Karmijn and Idared Apple
2.2. Morphology of the Skin of Susceptible and Non-Susceptible Cultivars
2.3. Skin Rheology of Developing Karmijn and Idared Apples
2.4. Rheology of the Skin of Susceptible and Non-Susceptible Cultivars
3. Discussion
3.1. In Early Fruit Development, the Epi- and Hypodermal Cells Were Larger and Their Sizes Were More Variable in Susceptible than in Non-Susceptible Cultivars
3.2. Fracture Strain of the Skin of Non-Susceptible Cultivars Was Higher than That of Susceptible Cultivars
3.3. Conclusions
4. Materials and Methods
4.1. Plant Materials
4.2. Fruit Surface Area Growth
4.3. Preparation of Microscopic Images
4.4. Epidermal and Hypodermal Cell Lengths, Heights, Tangential Areas, and Number of Cells Per Unit Area
4.5. Uniaxial Tensile Test
4.6. Data Analyses and Presentation
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cultivars | Fruit Surface Area (cm2) | Cell No. (×106 cm−2) | Cell No. (×106 Per Fruit) | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Epidermis | Hypodermis | Epidermis | Hypodermis | |||||||
Young | Mature | Young | Mature | Young | Mature | Young | Mature | Young | Mature | |
Russet Non-Susceptible | ||||||||||
Braeburn | 12.0 ± 0.4 | 189.5 ± 5.2 | 0.64 ± 0.05 | 0.24 ± 0.03 | 0.89 ± 0.03 | 0.33 ± 0.03 | 7.7 ± 0.6 | 45.6 ± 6.5 | 10.7 ± 0.4 | 62.0 ± 6.2 |
Granny Smith | 9.6 ± 0.2 | 182.3 ± 4.4 | 0.70 ± 0.07 | 0.21 ± 0.02 | 1.20 ± 0.09 | 0.46 ± 0.04 | 6.8 ± 0.6 | 38.9 ± 3.9 | 11.5 ± 0.9 | 84.4 ± 7.0 |
Idared | 18.5 ± 0.5 | 187.1 ± 3.2 | 0.80 ± 0.08 | 0.28 ± 0.02 | 1.07 ± 0.07 | 0.59 ± 0.05 | 14.3 ± 1.4 | 49.8 ± 2.7 | 19.2 ± 1.3 | 104.8 ± 8.9 |
Marina | 13.3 ± 0.4 | 188.1 ± 5.8 | 0.72 ± 0.04 | 0.32 ± 0.02 | 1.11 ± 0.04 | 0.47 ± 0.03 | 9.7 ± 0.5 | 61.1 ± 4.6 | 14.8 ± 0.6 | 89.2 ± 5.0 |
Mean | 13.4 ± 0.4b Z | 186.8 ± 2.2 a | 0.72 ± 0.03 a | 0.26 ± 0.01 a | 1.07 ± 0.04 a | 0.46 ± 0.03 a | 9.6 ± 0.7 a | 48.8 ± 2.7 a | 14.0 ± 0.8 a | 85.1 ± 4.4 a |
Russet Susceptible | ||||||||||
Boskoop Green | 16.2 ± 0.5 | 180.7 ± 6.4 | 0.44 ± 0.03 | 0.20 ± 0.01 | 0.73 ± 0.04 | 0.27 ± 0.03 | 7.1 ± 0.5 | 35.6 ± 1.7 | 11.8 ± 0.7 | 48.8 ± 4.6 |
Egremont Russet | 13.6 ± 0.5 | 111.7 ± 4.1 | 0.67 ± 0.05 | 0.31 ± 0.03 | 1.01 ± 0.06 | 0.57 ± 0.05 | 9.1 ± 0.7 | 34.2 ± 3.2 | 13.8 ± 0.8 | 64.0 ± 6.0 |
Kanada Renette | 19.4 ± 0.3 | 130.4 ± 4.2 | 0.44 ± 0.04 | 0.22 ± 0.03 | 0.89 ± 0.07 | 0.38 ± 0.03 | 8.6 ± 0.7 | 28.4 ± 3.4 | 17.3 ± 1.4 | 49.9 ± 3.9 |
Karmijn | 20.3 ± 0.6 | 146.0 ± 2.6 | 0.49 ± 0.06 | 0.28 ± 0.04 | 0.76 ± 0.03 | 0.35 ± 0.05 | 10.0 ± 1.1 | 41.3 ± 5.3 | 15.5 ± 0.6 | 52.7 ± 7.7 |
Superkalfs | 15.4 ± 0.4 | 171.7 ± 4.4 | 0.49 ± 0.04 | 0.24 ± 0.02 | 0.59 ± 0.05 | 0.20 ± 0.02 | 7.5 ± 0.6 | 41.6 ± 3.1 | 9.1 ± 0.7 | 35.1 ± 3.5 |
Mean | 17.3 ± 0.3 a | 143.0 ± 3.3 b | 0.51 ± 0.02 b | 0.25 ± 0.01 a | 0.80 ± 0.03 b | 0.36 ± 0.03 b | 8.5 ± 0.4 a | 36.2 ± 1.7 b | 13.5 ± 0.6 a | 50.1 ± 2.8 b |
Cultivars | Cell Height (μm) | Cell Length (μm) | Tangential Area (μm2) | Total Surface Area (μm2) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Epidermis | Hypodermis | Epidermis | Hypodermis | Epidermis | Hypodermis | Epidermis | Hypodermis | |||||||||
Young | Mature | Young | Mature | Young | Mature | Young | Mature | Young | Mature | Young | Mature | Young | Mature | Young | Mature | |
Russet Non-Susceptible | ||||||||||||||||
Braeburn | 16.2 ± 0.2 | 11.7 ± 0.3 | 12.3 ± 0.2 | 17.6 ± 0.5 | 12.6 ± 0.3 | 21.6 ± 0.8 | 21.2 ± 0.4 | 35.6 ± 1.2 | 171 ± 9 | 503 ± 36 | 502 ± 22 | 1535 ± 126 | 1167 ± 43 | 2018 ± 113 | 2084 ± 78 | 5914 ± 460 |
Granny Smith | 18.1 ± 0.3 | 15.7 ± 0.3 | 13.0 ± 0.2 | 15.2 ± 0.3 | 12.0 ± 0.3 | 22.0 ± 0.7 | 18.4 ± 0.4 | 29.9 ± 0.9 | 155 ± 8 | 522 ± 33 | 381 ± 17 | 1056 ± 69 | 1182 ± 44 | 2446 ± 119 | 1740 ± 61 | 4058 ± 242 |
Idared | 13.2 ± 0.2 | 11.3 ± 0.3 | 11.5 ± 0.2 | 13.6 ± 0.3 | 11.3 ± 0.4 | 19.2 ± 0.6 | 19.4 ± 0.5 | 26.7 ± 0.8 | 140 ± 9 | 393 ± 25 | 426 ± 21 | 831 ± 51 | 886 ± 41 | 1667 ± 88 | 1773 ± 73 | 3218 ± 177 |
Marina | 14.7 ± 0.2 | 11.0 ± 0.2 | 12.5 ± 0.2 | 12.9 ± 0.3 | 11.8 ± 0.4 | 17.7 ± 0.5 | 19.0 ± 0.4 | 29.2 ± 0.8 | 152 ± 9 | 329 ± 17 | 400 ± 17 | 964 ± 56 | 998 ± 42 | 1451 ± 63 | 1777 ± 63 | 3517 ± 181 |
Mean | 15.7 ± 0.1 b Z | 12.5 ± 0.2 a | 12.3 ± 0.1 b | 14.8 ± 0.2 a | 12.0 ± 0.2 b | 20.1 ± 0.3 a | 19.6 ± 0.2 b | 30.3 ± 0.5 b | 155 ± 4 b | 438 ± 15 a | 429 ± 10 b | 1091 ± 41 b | 1070 ± 22 b | 1904 ± 54 a | 1852 ± 35 b | 4158 ± 146 b |
Russet Susceptible | ||||||||||||||||
Boskoop Green | 20.3 ± 0.3 | 12.6 ± 0.2 | 16.0 ± 0.2 | 15.3 ± 0.4 | 15.2 ± 0.4 | 22.9 ± 0.7 | 23.5 ± 0.6 | 38.9 ± 1.4 | 250 ± 14 | 562 ± 34 | 627 ± 31 | 1805 ± 137 | 1745 ± 71 | 2278 ± 105 | 2812 ± 111 | 6233 ± 430 |
Egremont Russet | 15.7 ± 0.2 | 11.4 ± 0.2 | 10.9 ± 0.2 | 12.2 ± 0.2 | 12.3 ± 0.4 | 18.6 ± 0.6 | 20.0 ± 0.5 | 26.9 ± 0.9 | 162 ± 10 | 377 ± 25 | 451 ± 22 | 851 ± 71 | 1100 ± 44 | 1611 ± 80 | 1805 ± 73 | 3097 ± 217 |
Kanada Renette | 17.6 ± 0.4 | 12.4 ± 0.2 | 12.0 ± 0.2 | 12.1 ± 0.2 | 15.1 ± 0.4 | 22.1 ± 0.9 | 21.4 ± 0.5 | 32.5 ± 1.2 | 242 ± 14 | 540 ± 52 | 504 ± 25 | 1253 ± 108 | 1555 ± 66 | 2185 ± 153 | 2052 ± 81 | 4163 ± 307 |
Karmijn | 18.3 ± 0.3 | 12.7 ± 0.3 | 11.5 ± 0.2 | 15.8 ± 0.5 | 14.6 ± 0.5 | 19.4 ± 0.6 | 23.0 ± 0.6 | 34.6 ± 1.1 | 228 ± 13 | 408 ± 25 | 599 ± 32 | 1429 ± 101 | 1521 ± 61 | 1810 ± 87 | 2308 ± 107 | 5376 ± 372 |
Superkalfs | 22.6 ± 0.4 | 12.6 ± 0.2 | 17.3 ± 0.3 | 19.3 ± 0.7 | 14.4 ± 0.5 | 20.8 ± 0.7 | 26.2 ± 0.6 | 45.1 ± 1.5 | 227 ± 14 | 485 ± 39 | 768 ± 37 | 2380 ± 156 | 1769 ± 78 | 2039 ± 121 | 3417 ± 138 | 8694 ± 568 |
Mean | 19.0 ± 0.2 a | 12.3 ± 0.1 a | 13.6 ± 0.1 a | 15.0 ± 0.2 a | 14.3 ± 0.2 a | 20.7 ± 0.3 a | 22.8 ± 0.3 a | 35.5 ± 0.6 a | 221 ± 6 a | 469 ± 16 a | 591 ± 14 a | 1534 ± 55 a | 1540 ± 32 a | 1968 ± 50 a | 2490 ± 50 a | 5505 ± 190 a |
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Khanal, B.P.; Le, T.L.; Si, Y.; Knoche, M. Russet Susceptibility in Apple Is Associated with Skin Cells that Are Larger, More Variable in Size, and of Reduced Fracture Strain. Plants 2020, 9, 1118. https://doi.org/10.3390/plants9091118
Khanal BP, Le TL, Si Y, Knoche M. Russet Susceptibility in Apple Is Associated with Skin Cells that Are Larger, More Variable in Size, and of Reduced Fracture Strain. Plants. 2020; 9(9):1118. https://doi.org/10.3390/plants9091118
Chicago/Turabian StyleKhanal, Bishnu P., Thi Lieu Le, Yiru Si, and Moritz Knoche. 2020. "Russet Susceptibility in Apple Is Associated with Skin Cells that Are Larger, More Variable in Size, and of Reduced Fracture Strain" Plants 9, no. 9: 1118. https://doi.org/10.3390/plants9091118