Water Adsorption to Leaves of Tall Cryptomeria japonica Tree Analyzed by Infrared Spectroscopy under Relative Humidity Control
Abstract
:1. Introduction
2. Results
2.1. Changes with RH in IR Spectra
2.2. Changes with RH in IR Band Areas
2.3. Changes with RH in OH Band Components
2.4. Changes with RH in CH Band Area
2.5. Bulk Leaf Water Storage, Inorganic Element Concentrations, and Tissue Compositions of Leaves
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Preparation of Samples
5.2. Bulk Leaf Water Storage
5.3. Inorganic Elements of Leaves
5.4. IR Microspectroscopy with a Relative Humidity Control System
5.5. IR Band Area Changes with RH
5.6. Changes in OH Band Components with RH
5.7. Changes in CH Band Areas with RH
5.8. Data Analyses
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Larcher, W. Physiological Plant. Ecology: Ecophysiology and Stress Physiology of Functional Groups; Springer: Berlin, Germnay, 2003; ISBN 3540435166. [Google Scholar]
- Dewar, R.; Mauranen, A.; Mäkelä, A.; Hölttä, T.; Medlyn, B.; Vesala, T. New insights into the covariation of stomatal, mesophyll and hydraulic conductances from optimization models incorporating nonstomatal limitations to photosynthesis. New Phytol. 2018, 217, 571–585. [Google Scholar] [CrossRef] [Green Version]
- Brodribb, T.J.; Holbrook, N.M. Stomatal closure during leaf dehydration, correlation with other leaf physiological traits. Plant Physiol. 2003, 132, 2166–2173. [Google Scholar] [CrossRef] [Green Version]
- Bartlett, M.K.; Scoffoni, C.; Sack, L. The determinants of leaf turgor loss point and prediction of drought tolerance of species and biomes: A global meta-analysis. Ecol. Lett. 2012, 15, 393–405. [Google Scholar] [CrossRef] [PubMed]
- Scholz, F.; Phillips, N.; Bucci, S.; Meinzer, F.; Goldstein, G. Hydraulic capacitance: Biophysics and functional significance of internal water sources in relation to tree size. In Size- and Age-Related Changes in Tree Structure and Function; Meinzer, F.C., Lachenbruch, B., Dawson, T.E., Eds.; Springer: Dordrecht, The Netherlands, 2011; Volume 4, pp. 341–361. [Google Scholar]
- Woodruff, D.R.; Bond, B.J.; Meinzer, F.C. Does turgor limit growth in tall trees? Plant Cell Environ. 2004, 27, 229–236. [Google Scholar] [CrossRef]
- Ryan, M.G.; Yoder, B.J. Hydraulic limits to tree height and tree growth. Bioscience 1997, 47, 235–242. [Google Scholar] [CrossRef] [Green Version]
- Koch, G.W.; Sillett, S.C.; Jennings, G.M.; Davis, S.D. The limits to tree height. Nature 2004, 428, 851–854. [Google Scholar] [CrossRef] [PubMed]
- Ryan, M.G.; Phillips, N.; Bond, B.J. The hydraulic limitation hypothesis revisited. Plant Cell Environ. 2006, 29, 367–381. [Google Scholar] [CrossRef] [PubMed]
- Ishii, H.; Jennings, G.; Sillett, S.; Koch, G. Hydrostatic constraints on morphological exploitation of light in tall Sequoia sempervirens trees. Oecologia 2008, 156, 751–763. [Google Scholar] [CrossRef]
- Greenwood, M.S.; Ward, M.H.; Day, M.E.; Adams, S.L.; Bond, B.J. Age-related trends in red spruce foliar plasticity in relation to declining productivity. Tree Physiol. 2008, 28, 225–232. [Google Scholar] [CrossRef]
- Ishii, H.R.; Azuma, W.; Kuroda, K.; Sillett, S.C. Pushing the limits to tree height: Could foliar water storage compensate for hydraulic constraints in Sequoia sempervirens? Funct. Ecol. 2014, 28, 1087–1093. [Google Scholar] [CrossRef]
- Azuma, W.; Ishii, H.R.; Kuroda, K.; Kuroda, K. Function and structure of leaves contributing to increasing water storage with height in the tallest Cryptomeria japonica trees of Japan. Trees 2016, 30, 141–152. [Google Scholar] [CrossRef]
- Williams, C.B.; Reese Naesborg, R.; Dawson, T.E. Coping with gravity: The foliar water relations of giant sequoia. Tree Physiol. 2017, 37, 1312–1326. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Chin, A.R.O.; Sillett, S.C. Phenotypic plasticity of leaves enhances water-stress tolerance and promotes hydraulic conductivity in a tall conifer. Am. J. Bot. 2016, 103, 796–807. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhang, Y.-J.; Rockwell, F.E.; Wheeler, J.K.; Holbrook, N.M. Reversible deformation of transfusion tracheids in Taxus baccata is associated with a reversible decrease in leaf hydraulic conductance. Plant Physiol. 2014, 165, 1557–1565. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Martins, S.C.V.; Mcadam, S.A.M.; Deans, R.M.; Damatta, F.M.; Brodribb, T.J. Stomatal dynamics are limited by leaf hydraulics in ferns and conifers: Results from simultaneous measurements of liquid and vapour fluxes in leaves. Plant Cell Environ. 2016, 39, 694–705. [Google Scholar] [CrossRef] [Green Version]
- Ambrose, A.R.; Baxter, W.L.; Martin, R.E.; Francis, E.; Asner, G.P.; Nydick, K.R.; Dawson, T.E. Leaf- and crown-level adjustments help giant sequoias maintain favorable water status during severe drought. For. Ecol. Manag. 2018, 419–420, 257–267. [Google Scholar] [CrossRef]
- Zweifel, R.; Steppe, K.; Sterck, F.J. Stomatal regulation by microclimate and tree water relations: Interpreting ecophysiological field data with a hydraulic plant model. J. Exp. Bot. 2007, 58, 2113–2131. [Google Scholar] [CrossRef]
- Himeno, S.; Azuma, W.; Gyokusen, K.; Ishii, H.R. Leaf water maintains daytime transpiration in young Cryptomeria japonica trees. Tree Physiol. 2017, 37, 1394–1403. [Google Scholar] [CrossRef] [Green Version]
- Zweifel, R.; Hasler, R. Dynamics of water storage in mature subalpine Picea abies: Temporal and spatial patterns of change in stem radius. Tree Physiol. 2001, 21, 561–569. [Google Scholar] [CrossRef] [Green Version]
- Gente, R.; Koch, M. Monitoring leaf water content with THz and sub-THz waves. Plant Methods 2015, 11, 15. [Google Scholar] [CrossRef] [Green Version]
- Pagano, M.; Baldacci, L.; Ottomaniello, A.; De Dato, G.; Storchi, P.; Tredicucci, A.; Corona, P. THz water transmittance and leaf surface area: An effective nondestructive method for determining leaf water content. Sensors 2019, 19, 4838. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Nakamoto, K.; Margoshes, M.; Rundle, R.E. Stretching frequencies as a function of distances in hydrogen bonds. J. Am. Chem. Soc. 1955, 77, 6480–6486. [Google Scholar] [CrossRef]
- Azuma, W.; Nakashima, S.; Yamakita, E.; Ishii, H.R.; Kuroda, K. Water retained in tall Cryptomeria japonica leaves as studied by infrared micro-spectroscopy. Tree Physiol. 2017, 37, 1367–1378. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Kudo, S.; Ogawa, H.; Yamakita, E.; Watanabe, S.; Suzuki, T.; Nakashima, S. Adsorption of water to collagen as studied using infrared (IR) microscopy combined with relative humidity control system and quartz crystal microbalance. Appl. Spectrosc. 2017, 71, 1621–1632. [Google Scholar] [CrossRef] [PubMed]
- Yamakita, E.; Nakashima, S. Water retention of calcium-containing pectin studied by quartz crystal microbalance and infrared spectroscopy with a humidity control system. J. Agric. Food Chem. 2018, 66, 9344–9352. [Google Scholar] [CrossRef]
- Maréchal, Y. The Hydrogen Bond and the Water Molecule: The Physics and Chemistry of Water, Aqueous and Bio-Media; Elsevier: Amsterdam, The Netherlands, 2007; ISBN 9780444519573. [Google Scholar]
- Nakashima, S.; Kebukawa, Y.; Kitadai, N.; Igisu, M.; Matsuoka, N. Geochemistry and the origin of life: From extraterrestrial processes, chemical evolution on earth, fossilized life’s records, to natures of the extant life. Life 2018, 8, 39. [Google Scholar] [CrossRef] [Green Version]
- Demarty, M.; Morvan, C.; Thellier, M. Calcium and the cell wall. Plant Cell Environ. 1984, 7, 441–448. [Google Scholar] [CrossRef]
- Liners, F.; Letesson, J.J.; Didembourg, C.; Van Cutsem, P. Monoclonal antibodies against pectin: Recognition of a conformation induced by calcium. Plant Physiol. 1989, 91, 1419–1424. [Google Scholar] [CrossRef]
- Dunand, C.; Tognolli, M.; Overney, S.; Von Tobel, L.; De Meyer, M.; Simon, P.; Penel, C. Identification and characterisation of Ca 2 + -pectate binding peroxidases in Arabidopsis thaliana. J. Plant Physiol. 2002, 159, 1165–1171. [Google Scholar] [CrossRef]
- Wolf, S.; Greiner, S. Growth control by cell wall pectins. Protoplasma 2012, 249, 169–175. [Google Scholar] [CrossRef]
- Kudo, S.; Nakashima, S. Water adsorption with relative humidity changes for keratin and collagen as studied by infrared (IR) microspectroscopy. Ski. Res. Technol. 2019, 25, 258–269. [Google Scholar] [CrossRef] [PubMed]
- Kudo, S.; Nakashima, S. Changes in IR band areas and band shifts during water adsorption to lecithin and ceramide. Spectrochim. Acta-Part A Mol. Biomol. Spectrosc. 2020, 228, 117779. [Google Scholar] [CrossRef] [PubMed]
- Mizuno, K.; Ochi, T.; Shindo, Y. Hydrophobic hydration of acetone probed by nuclear magnetic resonance and infrared: Evidence for the interaction C-H⋯OH2. J. Chem. Phys. 1998, 109, 9502–9507. [Google Scholar] [CrossRef]
- Tomlinson-Phillips, J.; Davis, J.; Ben-Amotz, D.; Spångberg, D.; Pejov, L.; Hermansson, K. Structure and dynamics of water dangling OH bonds in hydrophobic hydration shells. Comparison of simulation and experiment. J. Phys. Chem. A 2011, 115, 6177–6183. [Google Scholar] [CrossRef]
- Davis, J.G.; Gierszal, K.P.; Wang, P.; Ben-Amotz, D. Water structural transformation at molecular hydrophobic interfaces. Nature 2012, 491, 582–585. [Google Scholar] [CrossRef] [PubMed]
- Norooz Oliaee, J.; Dehghany, M.; McKellar, A.R.W.; Moazzen-Ahmadi, N. High resolution infrared spectroscopy of carbon dioxide clusters up to (CO2)13. J. Chem. Phys. 2011, 135, 044315. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Maréchal, Y.; Chanzy, H. The hydrogen bond network in I(β) cellulose as observed by infrared spectrometry. J. Mol. Struct. 2000, 523, 183–196. [Google Scholar] [CrossRef]
- Fackler, K.; Stevanic, J.S.; Ters, T.; Hinterstoisser, B.; Schwanninger, M.; Salmén, L. Localisation and characterisation of incipient brown-rot decay within spruce wood cell walls using FT-IR imaging microscopy. Enzym. Microb. Technol. 2010, 47, 257–267. [Google Scholar] [CrossRef] [Green Version]
- Painter, P.; Snyder, R.; Starsinic, M.; Coleman, M.; Kuehn, D.; Davis, A. Concerning the application of FT-IR to the study of coal: A critical assessment of band assignments and the application of spectral analysis programs. Appl. Spectrosc. 1981, 35, 475–485. [Google Scholar] [CrossRef]
- Tonoue, R.; Katsura, M.; Hamamoto, M.; Bessho, H.; Nakashima, S. A method to obtain the absorption coefficient spectrum of single grain coal in the aliphatic C-H stretching region using infrared transflection microspectroscopy. Appl. Spectrosc. 2014, 68, 733–739. [Google Scholar] [CrossRef]
- Labbé, N.; Rials, T.; Kelley, S.; Cheng, Z.M.; Kim, J.Y.; Li, Y. FT-IR imaging and pyrolysis-molecular beam mass spectrometry: New tools to investigate wood tissues. Wood Sci. Technol. 2005, 39, 61–77. [Google Scholar] [CrossRef]
- Szymanska-Chargot, M.; Zdunek, A. Use of FT-IR spectra and PCA to the bulk characterization of cell wall residues of fruits and vegetables along a fraction process. Food Biophys. 2013, 8, 29–42. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Masuda, K.; Haramaki, T.; Nakashima, S.; Habert, B.; Martinez, I.; Kashiwabara, S. Structural change of water with solutes and temperature up to 100 °C in aqueous solutions as revealed by attenuated total reflectance infrared spectroscopy. Appl. Spectrosc. 2003, 57, 274–281. [Google Scholar] [CrossRef] [PubMed]
- Kataoka, Y.; Kitadai, N.; Hisatomi, O.; Nakashima, S. Nature of hydrogen bonding of water molecules in aqueous solutions of glycerol by attenuated total reflection (ATR) infrared spectroscopy. Appl. Spectrosc. 2011, 65, 436–441. [Google Scholar] [CrossRef] [PubMed]
- Kačuráková, M.; Wilson, R.H. Developments in mid-infrared FT-IR spectroscopy of selected carbohydrates. Carbohydr. Polym. 2001, 44, 291–303. [Google Scholar] [CrossRef]
- Ribeiro da Luz, B. Attenuated total reflectance spectroscopy of plant leaves: A tool for ecological and botanical studies. New Phytol. 2006, 172, 305–318. [Google Scholar] [CrossRef]
- Heraud, P.; Caine, S.; Sanson, G.; Gleadow, R.; Wood, B.R.; McNaughton, D. Focal plane array infrared imaging: A new way to analyse leaf tissue. New Phytol. 2007, 173, 216–225. [Google Scholar] [CrossRef]
- Filippov, M. Practical infrared spectroscopy of pectic substances. Top. Catal. 1992, 6, 115–142. [Google Scholar]
- Synytsya, A.; Copikova, J.; Matejka, P.; Machovic, V. Fourier transform Raman and infrared spectroscopy of pectins. Carbohydr. Polym. 2003, 54, 97–106. [Google Scholar] [CrossRef]
- Kacuráková, M.; Capek, P.; Sansinková, V.; Wellner, N.; Ebringerová, A. FT-IR study of plant cell wall model compounds: Pectic polysaccharides and hemicelluloses. Carbohydr. Polym. 2000, 43, 195–203. [Google Scholar] [CrossRef]
- Liang, C.Y.; Marchessault, R.H. Infrared spectra of crystalline polysaccharides. II. Native celluloses in the region from 640 to 1700 cm.1. J. Polym. Sci. 1959, 39, 269–278. [Google Scholar] [CrossRef]
- Olsson, A.M.; Salmén, L. The association of water to cellulose and hemicellulose in paper examined by FTIR spectroscopy. Carbohydr. Res. 2004, 339, 813–818. [Google Scholar] [CrossRef] [PubMed]
- Nakashima, S.; Spiers, C.; Mercury, L.; Fenter, P.; Hochella, M. (Eds.) Physicochemistry of Water in Geological and Biological Systems-Structures and Properties of Thin Aqueous Films; Universal Academy Press, Inc.: Tokyo, Japan, 2004. [Google Scholar]
- De Meer, S.; Spiers, C.J.; Nakashima, S. Structure and diffusive properties of fluid-filled grain boundaries: An in-situ study using infrared (micro) spectroscopy. Earth Planet. Sci. Lett. 2005, 232, 403–414. [Google Scholar] [CrossRef]
- Mochizuki, K.; Koga, K. Solid−liquid critical behavior of water in nanopores. Proc. Natl. Acad. Sci. USA 2015, 112, 8221–8226. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Aines, R.D.; Rossman, G.R. Water in minerals? A peak in the infrared. J. Geophys. Res. 1984, 89, 4059–4071. [Google Scholar] [CrossRef]
- Evert, R.F. Esau’s Plant Anatomy: Meristems, Cells, and Tissues of the Plant Body: Their Structure, Function, and Development; John Wiley & Sons: Hoboken, NJ, USA, 2006; ISBN 0470047372. [Google Scholar]
- Brodribb, T.J.; Holbrook, N.M.; Zwieniecki, M.A.; Palma, B.; Zwieniecki, A.; Michele, N.; Brodribb, J. Leaf hydraulic capacity in ferns, conifers and angiosperms: Impacts on photosynthetic maxima. New Phytol. 2005, 165, 839–846. [Google Scholar] [CrossRef]
- Tyree, M.T.; Hammel, H.T. The measurement of the turgor pressure and the water relations of plants by the pressure-bomb technique. J. Exp. Bot. 1972, 23, 267–282. [Google Scholar] [CrossRef]
- Schulte, P.J.; Hinckley, T.M. A comparison of pressure-volume curve data analysis techniques. J. Exp. Bot. 1985, 36, 1590–1602. [Google Scholar] [CrossRef]
- Bacelar, E.A.; Correia, C.M.; Moutinho-Pereira, J.M.; Goncalves, B.C.; Lopes, J.I.; Torres-Pereira, J.M.G. Sclerophylly and leaf anatomical traits of five field-grown olive cultivars growing under drought conditions. Tree Physiol. 2004, 24, 233–239. [Google Scholar] [CrossRef]
Labels | Peak Position (cm−1) | Assignment | Reference |
---|---|---|---|
a | 3340 | ν(O-H) | [40,41] |
b | 2920 | ν(C-H) | [42,43] |
c | 1740 | ν(C=O) (COOH) | [44,45] |
d | 1640 | δ(H-O-H) of water molecule | [46,47] |
e | 1610 | νas(COO−) | [48,49] |
f | 1515 | ν(C=C) | [50] |
g | 1450 | δas(CH3) | [50] |
h | 1415 | νs(COO−) | [45,48] |
i | 1370 | δas(CH3) | [50] |
j | 1315 | δ(O-H) | [40,41] |
k | 1263 | ν(C-O-C) | [51,52] |
l | 1229 | δ(O-H) | [51] |
m | 1150 | νas(C-O-C) | [40,41,53] |
n | 1105 | νs(COOH) | [49] |
o | 1075 | ν(C-O-C), δ(O-H) | [40,53] |
p | 1050 | ν(C-O) | [40,41] |
q | 1020 | ν(C-O) | [40,41,53] |
- | 700 | δ(OH) | [54,55] |
52 m | 19 m | ||
---|---|---|---|
Bulk leaf water storage | Cleaf (mol·m−2·MPa−1) | 1.4 (0.06) a | 0.9 (0.12) b |
Sleaf (g·H2O·m−2) | 226 (6.66) a | 125 (4.92) b | |
Inorganic element concentrations | Ca (mg·g−1) | 12.37 (0.74) a | 19.39 (1.36) b |
Mg (mg·g−1) | 2.07 (0.09) a | 3.31 (0.15) b | |
K (mg·g−1) | 6.01 (0.98) a | 5.71 (0.26) a | |
Na (mg·g−1) | 0.11 (0.03) a | 0.34 (0.24) a | |
P (mg·g−1) | 1.71 (0.19) a | 1.96 (0.15) a | |
Fe (mg·g−1) | 0.07 (0.02) a | 0.09 (0.01) a | |
Area percentages of tissues in leaf cross-sections | vascular bundle (%) | 3.2 | 2.3 |
transfusion tissue (%) | 15.6 | 5.8 | |
mesophyll (%) | 70.7 | 86.6 |
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Azuma, W.A.; Nakashima, S.; Yamakita, E.; Ohta, T. Water Adsorption to Leaves of Tall Cryptomeria japonica Tree Analyzed by Infrared Spectroscopy under Relative Humidity Control. Plants 2020, 9, 1107. https://doi.org/10.3390/plants9091107
Azuma WA, Nakashima S, Yamakita E, Ohta T. Water Adsorption to Leaves of Tall Cryptomeria japonica Tree Analyzed by Infrared Spectroscopy under Relative Humidity Control. Plants. 2020; 9(9):1107. https://doi.org/10.3390/plants9091107
Chicago/Turabian StyleAzuma, Wakana A., Satoru Nakashima, Eri Yamakita, and Tamihisa Ohta. 2020. "Water Adsorption to Leaves of Tall Cryptomeria japonica Tree Analyzed by Infrared Spectroscopy under Relative Humidity Control" Plants 9, no. 9: 1107. https://doi.org/10.3390/plants9091107