OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice
Abstract
:1. Introduction
2. Results
2.1. Expression Analysis of OsIRO3
2.2. Knockout of OsIRO3 Leads to Hypersensitivity to Fe Deficiency
2.3. Fe Concentration Analysis in WT and iro3 Mutants
2.4. Expression of Fe Homeostasis Genes in Roots of WT and iro3 Mutants
2.5. OsIRO3 Directly Binds to the Promoter of OsNAS3
2.6. Nicotianamine (NA) Analysis in WT and iro3 Mutants
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Plasmid Construction for Plant Transformation
4.3. Phenotypic Analysis of the OsIRO3 Knockout Lines
4.4. Fe Concentration Analysis
4.5. Real-Time PCR Analysis
4.6. Yeast-One-Hybrid Assay
4.7. Transient Expression Assays in Tobacco
4.8. ChIP-qPCR Assay
4.9. Measurement of Nicotianamine (NA) Concentration
4.10. Bioimaging of Fe by μ-XRF
4.11. Measurement of ROS Level in New Leaves
4.12. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, W.; Ye, J.; Ma, Y.; Wang, T.; Shou, H.; Zheng, L. OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice. Plants 2020, 9, 1095. https://doi.org/10.3390/plants9091095
Wang W, Ye J, Ma Y, Wang T, Shou H, Zheng L. OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice. Plants. 2020; 9(9):1095. https://doi.org/10.3390/plants9091095
Chicago/Turabian StyleWang, Wujian, Jun Ye, Yanran Ma, Ting Wang, Huixia Shou, and Luqing Zheng. 2020. "OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice" Plants 9, no. 9: 1095. https://doi.org/10.3390/plants9091095