Characterization of the Heat Shock Transcription Factor Family in Lycoris radiata and Its Potential Roles in Response to Abiotic Stresses
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization of LrHSF Proteins in L. radiata
2.2. Phylogenetic Analysis and Classification of LrHSF Proteins
2.3. Multiple Sequence Alignment and Conserved Motifs of LrHSF Proteins
2.4. Expression Patterns Analysis of LrHSF Genes in Different Tissues
2.5. Expression Analysis for LrHSFs Genes under Different Abiotic Stresses
2.6. LrHSF Proteins’ PPI Networks
2.7. Subcellular Localization of LrHSF Proteins
3. Discussion
3.1. Characterization of the LrHSF Gene Family in L. radiata
3.2. Expression Patterns and Function Prediction Analysis of LrHSFs
3.3. PPI Network Prediction and Validation of LrHSFs
4. Materials and Methods
4.1. Plant Materials and Plant Treatments
4.2. Identification and Sequence Analysis of LrHsf genes in L. radiata
4.3. LrHSF Proteins’ Phylogenetic Tree and Motif Analyses
4.4. Total RNA Extraction, cDNA Reverse Transcription, and qRT-PCR Analysis
4.5. Gene Cloning and Expression Vector Construction
4.6. LrHSF Proteins’ Subcellular Localization Analysis
4.7. PPI Network Prediction of LrHSF Proteins
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, N.; Shu, X.; Zhang, F.; Song, G.; Wang, Z. Characterization of the Heat Shock Transcription Factor Family in Lycoris radiata and Its Potential Roles in Response to Abiotic Stresses. Plants 2024, 13, 271. https://doi.org/10.3390/plants13020271
Wang N, Shu X, Zhang F, Song G, Wang Z. Characterization of the Heat Shock Transcription Factor Family in Lycoris radiata and Its Potential Roles in Response to Abiotic Stresses. Plants. 2024; 13(2):271. https://doi.org/10.3390/plants13020271
Chicago/Turabian StyleWang, Ning, Xiaochun Shu, Fengjiao Zhang, Guowei Song, and Zhong Wang. 2024. "Characterization of the Heat Shock Transcription Factor Family in Lycoris radiata and Its Potential Roles in Response to Abiotic Stresses" Plants 13, no. 2: 271. https://doi.org/10.3390/plants13020271