Effects of Exogenous Sodium Nitroprusside Spraying on Physiological Characteristics of Soybean Leaves at the Flowering Stage under Drought Stress
Abstract
:1. Introduction
2. Materials and Methods
Plant Materials and Test Design
3. Test Methods
3.1. Determination of the NO Content
3.2. Determination of Antioxidant Enzyme Activity
3.3. Determination of Contents of Osmoregulatory Substances
3.4. Determination of NiR and NR Activity
3.5. Determination of MDA Content
3.6. Data Analysis
4. Results and Analysis
4.1. Changes in the NO Content in Soybean Leaves
4.2. Changes in NiR and NR Activity in Soybean Leaves
4.3. Changes in Antioxidant Enzyme Activity in Soybean Leaves
4.4. Changes in Osmotic Adjustment Ability in Soybean Leaves
4.5. Changes in MDA Contents under Different SNP Treatments
4.6. Principal Component Analysis(PCA) and Multifactor Analysis of Variance
5. Discussion
6. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Constituents | PEG-6000 | SNP (μmol/L) | |
---|---|---|---|
Treatment | |||
CK | 0 | 0 | |
S0 | 15% | 0 | |
S50 | 15% | 50 | |
S100 | 15% | 100 | |
S250 | 15% | 250 | |
S500 | 15% | 500 |
Different Treatments | Days | Different Treatments and Days | |||||
---|---|---|---|---|---|---|---|
Variety | Index | F | Sig | F | Sig | F | Sig |
HN44 | NO | 173.001 | <0.01 | 396.733 | <0.01 | 18.203 | <0.01 |
NiR | 51.955 | <0.01 | 33.215 | <0.01 | 0.768 | 0.705 | |
NR | 166.089 | <0.01 | 24.92 | <0.01 | 3.143 | 0.001 | |
POD | 33.903 | <0.01 | 44.348 | <0.01 | 1.162 | 0.333 | |
CAT | 18.854 | <0.01 | 63.081 | <0.01 | 1.995 | 0.036 | |
SOD | 45.224 | <0.01 | 256.572 | <0.01 | 3.313 | 0.001 | |
APX | 25.104 | <0.01 | 116.587 | <0.01 | 2.415 | 0.011 | |
Pro | 757.019 | <0.01 | 1001.968 | <0.01 | 58.858 | <0.01 | |
SP | 750.399 | <0.01 | 1102.429 | <0.01 | 50.863 | <0.01 | |
SS | 130.832 | <0.01 | 129.369 | <0.01 | 5.773 | <0.01 | |
MDA | 82.765 | <0.01 | 188.203 | <0.01 | 10.8 | <0.01 | |
HN65 | NO | 127.699 | <0.01 | 297.073 | <0.01 | 10.057 | <0.01 |
NiR | 39.499 | <0.01 | 154.797 | <0.01 | 55.242 | <0.01 | |
NR | 56.685 | <0.01 | 19.408 | <0.01 | 1.245 | 0.273 | |
POD | 8.804 | <0.01 | 27.147 | <0.01 | 1.041 | 0.433 | |
CAT | 7.782 | <0.01 | 16.526 | <0.01 | 0.942 | 0.527 | |
SOD | 22.682 | <0.01 | 183.575 | <0.01 | 3.462 | 0.001 | |
APX | 12.8 | <0.01 | 23.472 | <0.01 | 0.768 | 0.704 | |
Pro | 257.096 | <0.01 | 107.024 | <0.01 | 7.367 | <0.01 | |
SP | 750.399 | <0.01 | 1102.429 | <0.01 | 50.863 | <0.01 | |
SS | 130.832 | <0.01 | 129.369 | <0.01 | 5.773 | <0.01 | |
MDA | 194.069 | <0.01 | 459.815 | <0.01 | 18.867 | <0.01 |
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Qu, Z.; Tian, Y.; Zhou, X.; Li, X.; Zhou, Q.; Wang, X.; Dong, S. Effects of Exogenous Sodium Nitroprusside Spraying on Physiological Characteristics of Soybean Leaves at the Flowering Stage under Drought Stress. Plants 2023, 12, 1598. https://doi.org/10.3390/plants12081598
Qu Z, Tian Y, Zhou X, Li X, Zhou Q, Wang X, Dong S. Effects of Exogenous Sodium Nitroprusside Spraying on Physiological Characteristics of Soybean Leaves at the Flowering Stage under Drought Stress. Plants. 2023; 12(8):1598. https://doi.org/10.3390/plants12081598
Chicago/Turabian StyleQu, Zhipeng, Yumei Tian, Xinyu Zhou, Xiaomei Li, Qi Zhou, Xiyue Wang, and Shoukun Dong. 2023. "Effects of Exogenous Sodium Nitroprusside Spraying on Physiological Characteristics of Soybean Leaves at the Flowering Stage under Drought Stress" Plants 12, no. 8: 1598. https://doi.org/10.3390/plants12081598