Identification and Characterization of Fusarium fujikuroi Pathotypes Responsible for an Emerging Bakanae Disease of Rice in India
Abstract
:1. Introduction
2. Results
2.1. Screening and Selection of Differential Rice Genotypes
2.2. Evaluation of Fusarium fujikuroi Isolates and Disease Pattern in a Set of Rice Genotypes
2.3. Multiple Comparisons among the Fusarium fujikuroi Isolates
2.4. Pathotype Classification of Fusarium fujikuroi Isolates
2.5. Pathotype Distribution in Basmati-Rice Growing States of India
2.6. Expression Analysis of Virulence-Related Genes in Fusarium fujikuroi
3. Discussion
4. Materials and Methods
4.1. Fungal Isolates and Rice Genotypes
4.2. Inoculation and Sowing Method
4.3. Screening of Rice Genotypes
4.4. Germination and Disease Incidence Scoring
4.5. Evaluation and Pathotyping of Fusarium fujikuroi Isolates
4.6. Selection of Virulence-Related Genes in Fusarium fujikuroi
4.7. Expression Analysis of Virulence-Related Genes in Fusarium fujikuroi
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Nirenberg, H. Untersuchungen uber die morphologische und biologische differenzierung in Fusarium-Sektion Liseola. Mitt.Biol Bundesansi. Land-Forstwirtsch. Berlin Dahlem 1976, 169, 1–117. [Google Scholar]
- Hori, S. Some observations on ‘Bakanae’disease of the rice plant. Mem. Agric. Res. Stn. 1898, 12, 110–119. [Google Scholar]
- Kanjanasoon, P. Studies on the Bakanae Disease of Rice in Thailand. Ph.D. Thesis, Tokyo University, Tokyo, Japan, 1965. [Google Scholar]
- Nelson, P.E.; Desjardins, A.E.; Plattner, R.D. Fumonisins, mycotoxins produced by Fusarium species: Biology, chemistry and significance. Annu. Rev. Phytopathol. 1993, 31, 233–252. [Google Scholar] [CrossRef] [PubMed]
- Cumagun, C.J.R.; Arcillas, E.; Gergon, E. UP-PCR analysis of the seedborne pathogen Fusarium fujikuroi causing bakanae disease in rice. Int. J. Agric. Biol. 2011, 13, 1029–1032. [Google Scholar]
- Bashyal, B.M.; Aggarwal, R.; Sharma, S.; Gupta, S.; Rawat, K.; Singh, D.; Krishnan, S.G. Occurrence, identification and pathogenicity of Fusarium species associated with bakanae disease of Basmati rice in India. Eur. J. Plant Pathol. 2016, 144, 457–466. [Google Scholar] [CrossRef]
- Bashyal, B.M.; Aggarwal, R. Molecular identification of Fusarium species associated with bakanae disease of rice (Oryza sativa L.) in India. Indian J. Agric. Sci. 2013, 83, 71–76. [Google Scholar]
- Bashyal, B.M.; Aggarwal, R.; Banerjee, S.; Gupta, S.; Sharma, S. Pathogenicity, ecology and genetic diversity of the Fusarium spp. associated with an emerging bakanae disease of rice (Oryza sativa L.) in India. In Microbial Diversity and Biotechnology in Food Security; Kharwar, R.N., Upadhyay, R., Dubey, N., Raghuwanshi, R., Eds.; Springer: New Delhi, India, 2014; pp. 307–314. [Google Scholar] [CrossRef]
- Sunani, S.K.; Bashyal, B.M.; Rawat, K.; Manjunatha, C.; Sharma, S.; Prakash, G.; Krishnan, S.G.; Singh, A.K.; Aggarwal, R. Development of PCR and loop mediated isothermal amplification assay for the detection of bakanae pathogen Fusarium fujikuroi. Eur. J. Plant Pathol. 2019, 154, 715–725. [Google Scholar] [CrossRef]
- Webster, R.K.; Gunnell, P.S. Compendium of Rice Diseases; The APS Press: St. Paul, MN, USA, 1992; p. 62. [Google Scholar]
- Desjardins, A.E.; Manandhar, H.K.; Plattner, R.D.; Manandhar, G.G.; Poling, S.M.; Maragos, C.M. Fusarium species from Nepalese rice and production of mycotoxins and gibberellic acid by selected species. Appl. Environ. Microbiol. 2000, 66, 1020–1025. [Google Scholar] [CrossRef] [Green Version]
- Bashyal, B.M. Etiology of an emerging disease: Bakanae of rice. Indian Phytopathol. 2018, 71, 485–494. [Google Scholar] [CrossRef]
- Anderson, L.; Webster, R. A comparison of assays for Gibberella fujikuroi and their ability to predict resulting bakanae from rice seed sources in California. Phytopathology 2005, 95 (Suppl. 4), 6. [Google Scholar] [CrossRef]
- Sunani, S.K.; Bashyal, B.M.; Kharayat, B.S.; Prakash, G.; Krishnan, S.G.; Aggarwal, R. Identification of rice seed infection routes of Fusarium fujikuroi inciting bakanae disease of rice. J. Plant Pathol. 2020, 102, 113–121. [Google Scholar] [CrossRef]
- Saremi, H.; Farrokhi, F. Study on bakanae disease of rice and evaluation of cultivars in Gilan and Zanjan provinces. In Proceedings of the Fourth International Iran and Russia Conference in Agriculture and Natural Resources, Shahrekord, Iran, 8–10 September, 2004; pp. 358–364. [Google Scholar]
- Hajra, K.K.; Ganguly, L.K.; Khatua, D.C. Bakanae disease of rice in West Bengal. J. Mycopathol. Res. 1994, 32, 95–99. [Google Scholar]
- Singh, N.I.; Devi, R.K.T.; Singh, L.N.K. Withering of growing shoot of rice caused by Fusarium moniliforme. Plant Dis. Res. 1996, 11, 99–100. [Google Scholar]
- Pavgi, M.S.; Singh, J. Bakanae and foot rot of rice in Uttar Pradesh, India. Plant Dis. Rep. 1964, 48, 340–342. [Google Scholar]
- Rathaiah, Y.; Das, G.R.; Singh, K.H.U. Estimation of yield loss and chemical control of bakanae disease of rice. Oryza 1991, 28, 509–512. [Google Scholar]
- Sunder, S.; Singh, R.; Dodan, D.S. Management of bakanae disease of rice caused by Fusarium moniliforme. Indian J. Agric. Sci. 2014, 84, 48–52. [Google Scholar]
- Gupta, A.K.; Singh, Y.; Jain, A.K.; Singh, D. Prevalence and incidence of bakanae disease of rice in Northern India. J. Agric. Res. 2014, 52, 233–237. [Google Scholar]
- Sunder, S. Vegetative compatibility, biosynthesis of GA3 and virulence of Fusarium moniliforme isolates from bakanae disease of rice. Plant Pathol. 1998, 47, 767–772. [Google Scholar] [CrossRef]
- Bashyal, B.M.; Aggarwal, R.; Sharma, S.; Gupta, S.; Singh, U.B. Single and combined effects of three Fusarium species associated with rice seeds on the severity of bakanae disease of rice. J. Plant Pathol. 2016, 98, 405–412. [Google Scholar]
- Luo, J.G. Studies on pathogenic Fusarium species of rice bakanae disease and strains. Chin. J. Rice Sci. 1995, 9, 119–122. [Google Scholar]
- Sharma, V.K.; Bagga, P.S. Pathogenic behaviour of Fusarium moniliforme isolates causing foot rot disease in Basmati rice. Plant Dis. Res. 2007, 22, 165–166. [Google Scholar]
- Jeon, Y.A.; Yu, S.H.; Lee, Y.Y.; Park, H.J.; Lee, S.; Sung, J.S.; Kim, Y.G.; Lee, H.S. Incidence, molecular characteristics and pathogenicity of Gibberella fujikuroi species complex associated with rice seeds from Asian countries. Mycobiology 2013, 41, 225–233. [Google Scholar] [CrossRef] [Green Version]
- Wiemann, P.; Sieber, C.M.K.; von Bargen, K.W.; Studt, L.; Niehaus, E.M.; Espino, J.J.; Huß, K.; Michielse, C.B.; Albermann, S.; Wagner, D.; et al. Deciphering the cryptic genome: Genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites. PLoS Pathog. 2013, 9, e1003475. [Google Scholar] [CrossRef] [Green Version]
- Bashyal, B.M.; Rawat, K.; Sharma, S.; Kulshrestha, D.; Singh, A.K.; Dubey, H.; Solanki, A.K.U.; Sharma, T.R.; Aggarwal, R. Whole genome sequencing of Fusarium fujikuroi provides insight into the role of secretory proteins and cell wall degrading enzymes in causing bakanae disease of rice. Front. Plant Sci. 2017, 8, 2013. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Piombo, E.; Rosati, M.; Sanna, M.; Mezzalama, M.; Gullino, M.L.; Spadaro, D. Sequencing of non-virulent strains of Fusarium fujikuroi reveals genes putatively involved in bakanae disease of rice. Fungal Genet. Biol. 2021, 156, 103622. [Google Scholar] [CrossRef] [PubMed]
- Fiyaz, R.A.; Krishnan, S.G.; Rajashekara, H.; Yadav, A.K.; Bashyal, B.M.; Bhowmick, P.K. Development of high throughput screening protocol and identification of novel sources of resistance against bakanae disease in rice (Oryza sativa L.). Indian J. Genet. Plant Breed. 2014, 74, 414–422. [Google Scholar] [CrossRef] [Green Version]
- Carter, L.L.A.; Leslie, J.F.; Webster, R.K. Population structure of Fusarium fujikuroi from California rice and water grass. Phytopathology 2008, 98, 992–998. [Google Scholar] [CrossRef] [Green Version]
- Niehaus, E.M.; Kim, H.K.; Munsterkotter, M.; Janevska, S.; Arndt, B.; Kalinina, S.A.; Houterman, P.M.; Alberti, I.; Tonti, S.; Sieber, C.M.K.; et al. Comparative genomics of geographically distant Fusarium fujikuroi isolates revealed two distinct pathotypes correlating with secondary metabolite profiles. PLoS Pathog. 2017, 13, e1006670. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Piombo, E.; Bosio, P.; Acquadro, A.; Abbruscato, P.; Spadaro, D. Different phenotypes, similar genomes: Three newly sequenced Fusarium fujikuroi strains induce different symptoms in rice depending on temperature. Phytopathology 2020, 110, 656–665. [Google Scholar] [CrossRef]
- Pouralibaba, H.R.; Rubiales, D.; Fondevilla, S. Identification of pathotypes in Fusarium oxysporum f.sp. lentis. Eur. J. Plant Pathol. 2016, 144, 539–549. [Google Scholar] [CrossRef]
- Adesioye, F.A.; Makhalanyane, T.P.; Biely, P.; Cowan, D.A. Phylogeny, classification and metagenomic bioprospecting of microbial acetyl xylan esterases. Enzym. Microb. Technol. 2016, 93, 79–91. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- De Sain, M.; Rep, M. The role of pathogen-secreted proteins in fungal vascular wilt diseases. Int. J. Mol. Sci. 2015, 16, 23970–23993. [Google Scholar] [CrossRef] [PubMed]
- Roy, A.; Jayaprakash, A.; Rajeswary, T.R.; Annamalai, A.; Lakshmi, P.T.V. Genome-wide annotation, comparison and functional genomics of carbohydrate active enzymes in legumes infecting Fusarium oxysporum formae speciales. Mycology 2020, 11, 56–70. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Wasmann, C.C.; VanEtten, H.D. Transformation-mediated chromosome loss and disruption of a gene for pisatin demethylase decrease the virulence of Nectria haematococca on pea. Mol. Plant Microbe Interact. 1996, 9, 793–803. [Google Scholar] [CrossRef]
- Rocha, L.O.; Laurence, M.H.; Ludowici, V.A.; Puno, V.I.; Lim, C.C.; Tesoriero, L.A.; Summerell, B.A.; Liew, E.C.Y. Putative effector genes detected in Fusarium oxysporum from natural ecosystems of Australia. Plant Pathol. 2016, 65, 914–929. [Google Scholar] [CrossRef] [Green Version]
- Bashyal, B.M.; Aggarwal, R.; Rawat, K.; Sharma, S.; Gupta, A.K.; Choudhary, R.; Bhat, J.; Gopala Krishnan, S.; Singh, A.K. Genetic diversity and population structure of Fusarium fujikuroi causing Bakanae, an emerging disease of rice in India. Indian J. Exp. Biol. 2020, 58, 45–52. [Google Scholar]
- International Network for Genetic Evaluation of Rice. Standard Evaluation System for Rice; IRRI: Manila, Philippines, 1996; p. 52. [Google Scholar]
- Livak, K.J.; Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 2001, 25, 402–408. [Google Scholar] [CrossRef]
- Microsoft Corporation. Microsoft Excel. 2019. Available online: https://office.microsoft.com/excel (accessed on 6 January 2022).
- Sheoran, O.P.; Tonk, D.S.; Kaushik, L.S.; Hasija, R.C.; Pannu, R.S. Statistical Software Package for Agricultural Research Workers. Recent Advances in Information Theory, Statistics and Computer Applications by DS Hooda and RC Hasija; Department of Mathematics Statistics, CCS HAU: Hisar, India, 1998; pp. 139–143. Available online: http://14.139.232.166/opstat (accessed on 15 January 2022).
Isolate Code | PB 1509 | PB 1121 | PB-1 | Kanak-Jeer | ANP 115-3-3-3-3 | C4-63G | K-14 | C101A51 | PB6 | BPT-5204 | PS-5 | IRG52 | DI * Mean |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
F201 | 13.79 | 49.97 | 1.17 | 13.56 | 31.68 | 0.00 | 61.15 | 0.00 | 10.60 | 66.81 | 36.44 | 4.55 | 24.14 |
F209 | 9.67 | 83.12 | 6.03 | 2.96 | 5.64 | 0.00 | 98.21 | 0.00 | 24.74 | 57.60 | 57.05 | 8.35 | 29.45 |
F219 | 25.50 | 48.11 | 20.10 | 6.25 | 78.39 | 2.80 | 35.82 | 0.00 | 35.86 | 50.26 | 0.00 | 8.30 | 25.95 |
F220 | 48.18 | 75.09 | 14.62 | 8.16 | 2.61 | 0.00 | 0.00 | 0.00 | 36.73 | 0.00 | 0.00 | 7.55 | 16.08 |
F224 | 65.85 | 58.82 | 6.89 | 7.30 | 23.75 | 0.00 | 0.00 | 0.00 | 43.42 | 0.00 | 0.00 | 4.80 | 17.57 |
F225 | 28.77 | 89.72 | 34.94 | 9.18 | 31.50 | 0.00 | 23.56 | 0.00 | 29.38 | 22.85 | 51.99 | 6.05 | 27.33 |
F226 | 73.94 | 80.67 | 26.52 | 4.98 | 29.73 | 0.00 | 27.06 | 0.00 | 34.10 | 28.63 | 0.00 | 0.00 | 25.47 |
F228 | 12.60 | 53.30 | 4.61 | 0.00 | 4.78 | 2.75 | 8.01 | 0.00 | 67.64 | 60.64 | 31.32 | 6.56 | 21.02 |
F235 | 27.92 | 83.67 | 0.00 | 7.01 | 0.00 | 0.00 | 4.74 | 0.00 | 51.00 | 17.06 | 59.00 | 3.78 | 21.18 |
F239 | 69.41 | 72.33 | 16.18 | 0.00 | 16.79 | 0.00 | 6.58 | 0.00 | 38.03 | 42.16 | 0.00 | 4.82 | 22.19 |
F250 | 24.09 | 76.70 | 27.13 | 13.57 | 0.00 | 0.00 | 0.00 | 0.00 | 17.90 | 51.50 | 0.00 | 5.40 | 18.02 |
F252 | 74.50 | 73.24 | 19.57 | 0.00 | 67.87 | 0.00 | 63.00 | 0.00 | 24.12 | 66.50 | 0.00 | 10.15 | 33.25 |
F253 | 25.22 | 86.50 | 31.82 | 6.52 | 39.49 | 0.00 | 69.87 | 0.00 | 65.06 | 51.32 | 63.50 | 6.32 | 37.13 |
F255 | 21.25 | 91.10 | 0.00 | 7.00 | 0.00 | 3.13 | 0.00 | 0.00 | 44.10 | 52.49 | 48.95 | 8.78 | 23.06 |
F258 | 46.00 | 68.35 | 2.84 | 6.05 | 49.35 | 0.00 | 95.00 | 0.00 | 11.00 | 37.85 | 22.50 | 3.79 | 28.56 |
F278 | 41.38 | 72.41 | 12.51 | 4.25 | 19.45 | 0.00 | 76.65 | 0.00 | 28.39 | 35.12 | 58.59 | 0.00 | 29.06 |
F344 | 46.22 | 59.17 | 11.99 | 5.65 | 6.08 | 3.90 | 97.50 | 0.00 | 67.69 | 36.20 | 0.00 | 3.50 | 28.16 |
F216a | 46.33 | 52.44 | 23.61 | 10.17 | 30.63 | 0.00 | 63.56 | 0.00 | 66.08 | 47.11 | 0.00 | 7.65 | 28.96 |
F225a | 46.31 | 80.89 | 5.10 | 1.80 | 0.00 | 0.00 | 4.79 | 0.00 | 19.00 | 0.00 | 94.90 | 4.00 | 21.40 |
F249a | 56.79 | 77.18 | 16.00 | 6.10 | 0.00 | 0.00 | 0.00 | 0.00 | 49.50 | 0.00 | 52.50 | 7.50 | 22.13 |
DI * Mean | 40.18 b | 71.64 a | 14.08 e | 6.02 f | 21.89 d | 0.63 g | 36.77 b | 0 g | 38.22 b | 36.2 b | 28.84 c | 5.59 f | 25.01 |
DI * pattern | S * | HS * | MS * | MR * | MS * | R * | S * | HR * | S * | S * | S * | MR * |
S. No. | Isolate No. | PB6 | PB-1 | C101A51 | PB1509 | IRG52 | DI * Mean Multiple Comparison |
---|---|---|---|---|---|---|---|
1 | F201 | 5.88 | 17.65 | 0.00 | 26.00 | 7.41 | 11.39 abcd |
2 | F204 | 7.69 | 6.07 | 0.00 | 26.67 | 7.69 | 9.62 abcd |
3 | F205 | 6.07 | 7.69 | 0.00 | 66.67 | 8.33 | 17.75 abcd |
4 | F206 | 27.78 | 33.33 | 0.00 | 76.47 | 12.00 | 29.92 abcd |
5 | F206a | 7.14 | 7.69 | 0.00 | 41.67 | 4.35 | 12.17 abcd |
6 | F207 | 5.88 | 23.53 | 0.00 | 43.75 | 0.00 | 14.63 abcd |
7 | F209 | 6.06 | 10.00 | 0.00 | 77.78 | 10.00 | 20.77 abcd |
8 | F210 | 5.88 | 38.89 | 0.00 | 46.15 | 11.54 | 20.49 abcd |
9 | F210a | 28.57 | 16.67 | 0.00 | 10.53 | 10.71 | 13.3 abcd |
10 | F211 | 22.22 | 11.11 | 0.00 | 26.32 | 0.00 | 11.93 abcd |
11 | F212a | 7.14 | 16.67 | 0.00 | 63.16 | 7.69 | 18.93 abcd |
12 | F213 | 46.15 | 57.14 | 5.88 | 65.00 | 12.00 | 37.24 a |
13 | F213a | 22.22 | 13.33 | 0.00 | 50.00 | 3.45 | 17.8 abcd |
14 | F214 | 5.88 | 8.33 | 0.00 | 7.14 | 3.45 | 4.96 bcd |
15 | F216 | 6.09 | 6.05 | 0.00 | 20.00 | 20.00 | 10.43 abcd |
16 | F216a | 7.69 | 26.32 | 0.00 | 53.33 | 12.00 | 19.87 abcd |
17 | F217 | 11.76 | 10.00 | 0.00 | 35.71 | 0.00 | 11.5 abcd |
18 | F217a | 21.43 | 26.67 | 0.00 | 35.71 | 16.00 | 19.96 abcd |
19 | F218 | 6.05 | 6.09 | 0.00 | 7.69 | 12.00 | 6.37 abcd |
20 | F218a | 11.11 | 12.50 | 0.00 | 31.25 | 10.00 | 12.97 abcd |
21 | F219 | 11.11 | 10.53 | 0.00 | 81.25 | 14.81 | 23.54 abcd |
22 | F220 | 62.50 | 40.00 | 0.00 | 56.25 | 10.71 | 33.89 abcd |
23 | F222 | 15.38 | 6.07 | 0.00 | 94.12 | 0.00 | 23.11 abcd |
24 | F223 | 14.29 | 3.57 | 0.00 | 84.62 | 0.00 | 20.49 abcd |
25 | F223a | 6.06 | 7.69 | 0.00 | 60.00 | 12.00 | 17.15 abcd |
26 | F224 | 13.33 | 7.69 | 0.00 | 93.33 | 7.14 | 24.3 abcd |
27 | F225 | 5.56 | 5.56 | 0.00 | 30.77 | 7.41 | 9.86 abcd |
28 | F225a | 5.88 | 33.33 | 0.00 | 22.22 | 4.00 | 13.09 abcd |
29 | F226 | 50.00 | 27.27 | 0.00 | 93.33 | 0.00 | 34.12 abc |
30 | F228 | 7.14 | 16.67 | 0.00 | 46.67 | 7.41 | 15.58 abcd |
31 | F228a | 20.00 | 7.69 | 0.00 | 30.77 | 0.00 | 11.69 abcd |
32 | F230 | 20.00 | 0.00 | 0.00 | 36.36 | 0.00 | 11.27 abcd |
33 | F231 | 6.07 | 23.08 | 0.00 | 42.86 | 0.00 | 14.4 abcd |
34 | F232 | 12.50 | 45.45 | 0.00 | 35.71 | 20.69 | 22.87 abcd |
35 | F233 | 40.00 | 25.00 | 0.00 | 92.31 | 13.33 | 34.13 abc |
36 | F234 | 42.86 | 33.33 | 0.00 | 45.45 | 7.41 | 25.81 abcd |
37 | F235 | 23.53 | 25.00 | 0.00 | 11.11 | 3.57 | 12.64 abcd |
38 | F237a | 5.88 | 26.67 | 0.00 | 36.36 | 10.71 | 15.93 abcd |
39 | F239 | 66.67 | 41.18 | 0.00 | 66.67 | 7.41 | 36.38 ab |
40 | F240 | 53.85 | 41.18 | 0.00 | 60.00 | 10.34 | 33.07 abcd |
41 | F241 | 5.88 | 5.26 | 0.00 | 68.75 | 3.70 | 16.72 abcd |
42 | F242 | 6.06 | 5.26 | 0.00 | 92.86 | 6.06 | 22.05 abcd |
43 | F242a | 16.67 | 25.00 | 0.00 | 92.31 | 15.38 | 29.87 abcd |
44 | F244 | 29.41 | 11.76 | 5.26 | 62.50 | 10.71 | 23.93 abcd |
45 | F245a | 9.09 | 11.76 | 0.00 | 33.33 | 0.00 | 10.84 abcd |
46 | F246 | 27.27 | 35.71 | 0.00 | 46.67 | 18.18 | 25.57 abcd |
47 | F247 | 31.25 | 41.67 | 0.00 | 27.27 | 10.00 | 22.04 abcd |
48 | F249 | 23.53 | 33.33 | 0.00 | 36.84 | 3.57 | 19.46 abcd |
49 | F249a | 23.08 | 5.26 | 0.00 | 22.22 | 7.41 | 11.59 abcd |
50 | F250 | 23.08 | 15.38 | 5.26 | 44.44 | 7.14 | 19.06 abcd |
51 | F252 | 16.67 | 5.58 | 0.00 | 7.77 | 12.50 | 8.5 abcd |
52 | F253 | 21.43 | 15.38 | 0.00 | 33.33 | 3.57 | 14.74 abcd |
53 | F254 | 35.71 | 21.43 | 0.00 | 21.43 | 17.86 | 19.29 abcd |
54 | F255 | 14.29 | 6.06 | 0.00 | 22.22 | 11.11 | 10.74 abcd |
55 | F255a | 6.07 | 5.26 | 0.00 | 5.26 | 0.00 | 3.32 cd |
56 | F256 | 23.53 | 7.69 | 0.00 | 93.33 | 7.41 | 26.39 abcd |
57 | F258 | 9.09 | 5.88 | 0.00 | 50.00 | 3.33 | 13.66 abcd |
58 | F259 | 21.43 | 12.50 | 0.00 | 40.00 | 7.69 | 16.32 abcd |
59 | F261 | 31.25 | 33.33 | 0.00 | 41.67 | 3.57 | 21.96 abcd |
60 | F263 | 25.00 | 7.69 | 0.00 | 12.50 | 25.00 | 14.04 abcd |
61 | F267 | 6.07 | 7.14 | 0.00 | 54.55 | 11.54 | 15.86 abcd |
62 | F268 | 23.53 | 12.50 | 0.00 | 31.25 | 7.41 | 14.94 abcd |
63 | F272 | 28.57 | 5.88 | 0.00 | 33.33 | 4.00 | 14.36 abcd |
64 | F274 | 8.33 | 23.08 | 6.07 | 21.43 | 18.52 | 15.48 abcd |
65 | F277 | 28.57 | 21.43 | 6.07 | 21.43 | 20.83 | 19.67 abcd |
66 | F278 | 7.69 | 10.00 | 0.00 | 33.33 | 0.00 | 10.21 abcd |
67 | F278a | 20.00 | 6.25 | 0.00 | 33.33 | 3.84 | 12.68 abcd |
68 | F279 | 8.33 | 4.00 | 0.00 | 33.33 | 3.85 | 9.9 abcd |
69 | F280 | 8.33 | 5.26 | 0.00 | 8.33 | 20.69 | 8.52 abcd |
70 | F282 | 25.00 | 6.25 | 0.00 | 35.71 | 25.92 | 18.58 abcd |
71 | F284 | 7.14 | 3.33 | 0.00 | 16.67 | 22.22 | 9.87 abcd |
72 | F285 | 35.29 | 10.00 | 0.00 | 26.32 | 0.00 | 14.32 abcd |
73 | F287 | 18.18 | 6.06 | 0.00 | 9.09 | 3.57 | 7.38 abcd |
74 | F288 | 14.29 | 7.69 | 0.00 | 21.43 | 4.00 | 9.48 abcd |
75 | F289 | 18.75 | 33.33 | 0.00 | 9.09 | 24.14 | 17.06 abcd |
76 | F291 | 46.67 | 6.07 | 0.00 | 8.33 | 21.43 | 16.5 abcd |
77 | F294 | 0.00 | 0.00 | 0.00 | 33.33 | 3.85 | 7.44 abcd |
78 | F295 | 25.00 | 3.33 | 0.00 | 7.69 | 7.41 | 8.69 abcd |
79 | F297 | 0.00 | 0.00 | 0.00 | 15.38 | 6.90 | 4.46 cd |
80 | F298 | 7.69 | 16.67 | 0.00 | 11.76 | 0.00 | 7.22 abcd |
81 | F300 | 18.18 | 7.69 | 0.00 | 27.27 | 3.70 | 11.37 abcd |
82 | F309 | 26.66 | 5.88 | 0.00 | 33.33 | 3.44 | 13.86 abcd |
83 | F339 | 25.00 | 23.53 | 0.00 | 10.00 | 17.86 | 15.28 abcd |
84 | F340 | 7.14 | 9.09 | 0.00 | 20.00 | 29.63 | 13.17 abcd |
85 | F341 | 18.75 | 9.09 | 0.00 | 10.00 | 11.11 | 9.79 abcd |
86 | F342 | 28.57 | 25.00 | 0.00 | 10.00 | 14.81 | 15.68 abcd |
87 | F343 | 26.32 | 8.33 | 5.88 | 40.00 | 7.41 | 17.59 abcd |
88 | F344 | 0.00 | 0.00 | 0.00 | 7.69 | 3.57 | 2.25 d |
89 | Bundi-1 | 18.75 | 12.50 | 0.00 | 50.00 | 0.00 | 16.25 abcd |
90 | Bundi-2 | 20.00 | 25.00 | 0.00 | 38.46 | 10.71 | 18.84 abcd |
91 | Bundi-3 | 45.45 | 45.45 | 0.00 | 10.00 | 3.57 | 20.9 abcd |
92 | Bundi-4 | 42.86 | 18.18 | 0.00 | 11.76 | 7.41 | 16.04 abcd |
93 | Bundi-5 | 11.76 | 33.33 | 0.00 | 55.56 | 7.14 | 21.56 abcd |
94 | NIB * | 22.22 | 5.88 | 0.00 | 75.00 | 0.00 | 20.62 abcd |
95 | F1121 | 40.00 | 18.18 | 0.00 | 21.43 | 0.00 | 15.92 abcd |
96 | F1728 | 23.53 | 10.00 | 0.00 | 78.57 | 0.00 | 22.42 abcd |
97 | Bundi | 29.41 | 13.33 | 0.00 | 45.45 | 0.00 | 17.64 abcd |
DI * Mean | 19.52 | 16.01 | 0.35 | 39.13 | 8.35 | 16.67 |
Pathotypes | Rice Genotypes | Isolates | Total Isolates | ||||
---|---|---|---|---|---|---|---|
PB6 | PB1 | C101A51 | PB1509 | IRG52 | |||
1 | S | S | HR | S | S | F210a, F217a, F218a, F228, F232, F234, F246, F247, F254, F259, F263, F268, F289, F339, F340, F341, F342, Bundi-2, Bundi-4 | 19 |
2 | S | S | HR | S | HR | F211, F217, F226, F228a, F230, F245a, F278, F285, F298, Bundi, Bundi-1, F1121, F1728 | 13 |
3 | S | S | HR | S | R | F206a, F213a, F235, F249, F253, F261, F288, F300, Bundi-3 | 9 |
4 | S | S | HR | HS | S | F206, F212a, F216a, F219, F224, F233, F242a, F256, Bundi-5 | 9 |
5 | S | R | HR | S | S | F204, F249a, F252, F255, F255a, F280, F282, F284, F291, F295 | 10 |
6 | R | S | HR | HS | S | F205, F209, F223a, F267 | 4 |
7 | S | R | HR | S | R | F258, F272, F278a, F279, F287, F309 | 6 |
8 | S | S | R | S | S | F213, F244, F250, F274, F277, F343 | 6 |
9 | R | S | HR | S | S | F201, F210, F237a | 3 |
10 | S | R | HR | HS | HR | F222, F223, NIB | 3 |
11 | R | R | HR | S | S | F216, F218, F225, F225a | 4 |
12 | HS | S | HR | HS | S | F220, F239, F240 | 3 |
13 | HR | HR | HR | S | R | F294, F297, F344 | 3 |
14 | R | S | HR | S | HR | F207, F214, F231 | 3 |
15 | R | R | HR | HS | R | F241, F242 | 2 |
97 |
Geographical Distribution | Pathotypes | Total Isolates | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | ||
Punjab | 9 | 7 | 6 | 6 | 4 | 3 | 5 | 4 | 2 | 2 | 4 | 2 | 2 | 1 | 2 | 59 |
Haryana | 3 | 0 | 2 | 1 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 12 |
Uttar Pradesh | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 10 |
Uttarakhand | 3 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 8 |
Rajasthan | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 |
Delhi | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Total isolates | 19 | 13 | 9 | 9 | 10 | 4 | 6 | 6 | 3 | 3 | 4 | 3 | 3 | 3 | 2 | 97 |
Disease Rating Scale | Percent Infected Seedlings | Disease Reaction |
---|---|---|
0 | 0% | Highly resistant (HR) |
1 | ≤1% | Resistant (R) |
3 | 1–6% | Moderately resistant (MR) |
5 | 6–25% | Moderately susceptible (MS) |
7 | 25–50% | Susceptible (S) |
9 | 50–100% | Highly susceptible (HS) |
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Tadasanahaller, P.S.; Bashyal, B.M.; Yadav, J.; Krishnan Subbaiyan, G.; Ellur, R.K.; Aggarwal, R. Identification and Characterization of Fusarium fujikuroi Pathotypes Responsible for an Emerging Bakanae Disease of Rice in India. Plants 2023, 12, 1303. https://doi.org/10.3390/plants12061303
Tadasanahaller PS, Bashyal BM, Yadav J, Krishnan Subbaiyan G, Ellur RK, Aggarwal R. Identification and Characterization of Fusarium fujikuroi Pathotypes Responsible for an Emerging Bakanae Disease of Rice in India. Plants. 2023; 12(6):1303. https://doi.org/10.3390/plants12061303
Chicago/Turabian StyleTadasanahaller, Prashantha S., Bishnu Maya Bashyal, Jagdish Yadav, Gopala Krishnan Subbaiyan, Ranjith K. Ellur, and Rashmi Aggarwal. 2023. "Identification and Characterization of Fusarium fujikuroi Pathotypes Responsible for an Emerging Bakanae Disease of Rice in India" Plants 12, no. 6: 1303. https://doi.org/10.3390/plants12061303