BrCYP71A15 Negatively Regulates Hg Stress Tolerance by Modulating Cell Wall Biosynthesis in Yeast
Abstract
:1. Introduction
2. Results
2.1. The Effect of Hg Stress on the Chlorophyll Content
2.2. BrCYP71A15 Gene Induced by Hg Stress in Chinese Cabbage
2.3. Expression Patterns of the BrCYP71A15 Gene under Different Abiotic Stresses
2.4. Expression Patterns of the BrCYP71A15 Gene in Different Tissues of Chinese Cabbage
2.5. BrCYP71A15 Protein–Protein Association Network
2.6. BrCYP71A15 Overexpression in Response to Abiotic Stresses in Yeast
2.7. Subcellular Localization of the BrCYP71A15 Gene
2.8. Cell Wall Biosynthesis Gene
3. Discussion
4. Materials and Methods
4.1. Chlorophyll Contents
4.2. Yeast Constructs
4.3. Hg Concentration in Yeast Cells
4.4. Tolerance Assay and Growth Curve
4.5. Total RNA Extraction and qRT-PCR Analysis
4.6. Statistical Analysis
5. Summary
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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miRNA | Gene | Length | Start | End | miRNA Aligned Fragment | Alignment | Targeted Fragment | Inhibition |
---|---|---|---|---|---|---|---|---|
ath-miR5644 | Bra033509 | 20 | 81 | 100 | GUGGGUUGCGGAUAACGGUA | ::: :: :::.::::.:: | CACCUCUAACCGUAACCUAC | Cleavage |
ath-miR8183 | Bra033509 | 21 | 232 | 252 | UUUAGUUGACGGAAUUGUGGC | ....:.::.:::::.::.: | CUUGUAGUUUCGUCAGCUGAC | Cleavage |
ath-miR854a | Bra033509 | 21 | 217 | 237 | GAUGAGGAUAGGGAGGAGGAG | .: : ::::::::::..: | GGUCGCGUCCCUAUCCUUGUA | Cleavage |
ath-miR854b | Bra033509 | 21 | 217 | 237 | GAUGAGGAUAGGGAGGAGGAG | .: : ::::::::::..: | GGUCGCGUCCCUAUCCUUGUA | Cleavage |
ath-miR854c | Bra033509 | 21 | 217 | 237 | GAUGAGGAUAGGGAGGAGGAG | .: : ::::::::::..: | GGUCGCGUCCCUAUCCUUGUA | Cleavage |
ath-miR854d | Bra033509 | 21 | 217 | 237 | GAUGAGGAUAGGGAGGAGGAG | .: : ::::::::::..: | GGUCGCGUCCCUAUCCUUGUA | Cleavage |
ath-miR854e | Bra033509 | 21 | 217 | 237 | GAUGAGGAUAGGGAGGAGGAG | .: : ::::::::::..: | GGUCGCGUCCCUAUCCUUGUA | Cleavage |
ath-miR2934-5p | Bra033509 | 21 | 733 | 753 | UCUUUCUGCAAACGCCUUGGA | :..: :: :::: .::::.:: | UUUAUGGAGUUUCUAGAAGGA | Cleavage |
ath-miR426 | Bra033509 | 21 | 1268 | 1288 | UUUUGGAAAUUUGUCCUUACG | : :::::::. ::.:::. | UUUCAGGACAAGAUUUCAAGU | Cleavage |
ath-miR5632-3p | Bra033509 | 21 | 808 | 828 | UUGGAUUUAUAGUUGGAUAAG | ::: : ..:: ::::.::: | GAUAUGCUGUUACAAAUUCAA | Cleavage |
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Anwar, A.; Zhang, S.; Wang, L.; He, L.; Gao, J. BrCYP71A15 Negatively Regulates Hg Stress Tolerance by Modulating Cell Wall Biosynthesis in Yeast. Plants 2023, 12, 723. https://doi.org/10.3390/plants12040723
Anwar A, Zhang S, Wang L, He L, Gao J. BrCYP71A15 Negatively Regulates Hg Stress Tolerance by Modulating Cell Wall Biosynthesis in Yeast. Plants. 2023; 12(4):723. https://doi.org/10.3390/plants12040723
Chicago/Turabian StyleAnwar, Ali, Shu Zhang, Lixia Wang, Lilong He, and Jianwei Gao. 2023. "BrCYP71A15 Negatively Regulates Hg Stress Tolerance by Modulating Cell Wall Biosynthesis in Yeast" Plants 12, no. 4: 723. https://doi.org/10.3390/plants12040723