Exogenous SA Applications Alleviate Salinity Stress via Physiological and Biochemical changes in St John’s Wort Plants
Abstract
:1. Introduction
2. Results
2.1. Effects of SA Concentration on the Chlorophyll Content of St. John’s Wort Leaves under Salt Stress
2.2. Recovery Effect of Exogenous SA on St. John’s Wort Seedlings under Salinity Stress
2.2.1. Plant Growth Characteristics and Chlorophyll Content
2.2.2. Efficiency of the Photosystem (Fv/Fm, Fv/Fo, PI)
2.2.3. Influence of SA Application on Regulation of Endogenous ABA Content under Salinity Stress
2.2.4. Estimation of Amino Acid Levels
2.2.5. Determination of Enzymatic and Nonenzymatic Antioxidant Activities (DPPH, SOD, Phenol, and Flavonoid)
2.3. Effects of SA Application on Regulation of Endogenous Melatonin and Transcriptional Response under Salinity Stress
3. Discussion
4. Materials and Methods
4.1. Experimental Design and Treatments
4.1.1. Leaf Disk Bioassay
4.1.2. Plant Experiment
4.2. Biochemical Analysis of Plants
4.2.1. Chlorophyll Content and the Photosystem (Fv/Fm, Fv/Fo, PI)
4.2.2. Quantitation of ABA Content
4.2.3. Measurement of Amino Acid Content
4.2.4. Determination of Enzymatic and Nonenzymatic Antioxidant Activities
4.2.5. Quantification of Melatonin Content
4.2.6. cDNA Synthesis and Real-Time PCR Analysis of Enzymes of the Melatonin Pathway
4.3. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | Plant Height (cm) | Root (cm) | Total Plant Fresh Weight (g) | Stem Diameter (cm) | No. Leaf |
---|---|---|---|---|---|
Control | 10.5 ± a | 5.2 ± ab | 8.3 ± a | 0.37 ± a | 193 ± a |
SA | 10.6 ± a | 5.7 ± a | 8.7 ± a | 0.35 ± ab | 194 ± a |
Salt | 6.6 ± c | 3.5 ± c | 4.8 ± c | 0.2 ± c | 92 ± b |
SA + Salt | 8.2 ± b | 4.7 ± b | 6.5 ± b | 0.31 ± b | 135 ± c |
Amino Acid | Treatment | |||
---|---|---|---|---|
mg/g | Control | SA | Salt | Salt → SA |
Asp | 3.73 ± 0.0 a | 3.24 ± 0.0 b | 3.73 ± 0.0 a | 3.15 ± 0.0 b |
Thr | 1.93 ± 0.0 b | 1.67 ± 0.0 a | 1.89 ± 0.0 b | 1.64 ± 0.0 c |
Ser | 1.8 ± 0.0 a | 1.58 ± 0.0 b | 1.87 ± 0.0 a | 1.64 ± 0.0 b |
Glu | 6.83 ± 0.0 a | 6.16 ± 0.0 b | 6.25 ± 0.0 a | 5.51 ± 0.0 b |
Gly | 2.02 ± 0.0 a | 1.74 ± 0.0 bc | 1.83 ± 0.0 b | 1.72 ± 0.0 c |
Ala | 2.43 ± 0.0 a | 2.11 ± 0.0 b | 2.55 ± 0.0 a | 2.05 ± 0.0 b |
Cys | 0.14 ± 0.0 a | 0.13 ± 0.0 a | 0.19 ± 0.0 a | 0.15 ± 0.0 a |
Val | 2.17 ± 0.0 a | 1.89 ± 0.0 b | 2.12 ± 0.0 a | 1.82 ± 0.0 c |
Met | 0.0 8 ± 0.0 a | 0.0 6 ± 0.0 a | 0.0 7 ± 0.0 a | 0.0 7 ± 0.0 a |
Ile | 1.63 ± 0.0 a | 1.42 ± 0.0 c | 1.56 ± 0.0 b | 1.34 ± 0.0 d |
Leu | 3.43 ± 0.0 a | 2.96 ± 0.0 c | 3.18 ± 0.0 b | 2.81 ± 0.0 c |
Tyr | 1.04 ± 0.0 a | 0.86 ± 0.0 c | 1.05 ± 0.0 ab | 0.92 ± 0.0 bc |
Phe | 2.07 ± 0.0 a | 1.81 ± 0.0 b | 1.92 ± 0.0 b | 1.74 ± 0.0 c |
Lys | 3.05 ± 0.0 a | 2.61 ± 0.0 c | 2.69 ± 0.0 b | 2.6 ± 0.0 c |
His | 1.11 ± 0.0 a | 0.96 ± 0.0 b | 1.16 ± 0.0 a | 0.91 ± 0.0 b |
Arg | 6.57 ± 0.0 a | 6.07 ± 0.0 b | 5.31 ± 0.0 c | 4.71 ± 0.0 d |
Pro | 3.2 ± 0.0 a | 2.87 ± 0.0 d | 5.21 ± 0.0 a | 2.95 ± 0.0 c |
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Kwon, E.-H.; Adhikari, A.; Imran, M.; Lee, D.-S.; Lee, C.-Y.; Kang, S.-M.; Lee, I.-J. Exogenous SA Applications Alleviate Salinity Stress via Physiological and Biochemical changes in St John’s Wort Plants. Plants 2023, 12, 310. https://doi.org/10.3390/plants12020310
Kwon E-H, Adhikari A, Imran M, Lee D-S, Lee C-Y, Kang S-M, Lee I-J. Exogenous SA Applications Alleviate Salinity Stress via Physiological and Biochemical changes in St John’s Wort Plants. Plants. 2023; 12(2):310. https://doi.org/10.3390/plants12020310
Chicago/Turabian StyleKwon, Eun-Hae, Arjun Adhikari, Muhammad Imran, Da-Sol Lee, Chung-Yeol Lee, Sang-Mo Kang, and In-Jung Lee. 2023. "Exogenous SA Applications Alleviate Salinity Stress via Physiological and Biochemical changes in St John’s Wort Plants" Plants 12, no. 2: 310. https://doi.org/10.3390/plants12020310