Humic Substances Isolated from Recycled Biomass Trigger Jasmonic Acid Biosynthesis and Signalling
Abstract
:1. Introduction
2. Results
2.1. Humic Acids Characteristics
2.2. Plant Phenotypes and Growth
2.3. JERE::GUS Gene Reporter
2.4. JA Synthesis and Signalling
3. Discussion
4. Materials and Methods
4.1. Vermicompost Production
4.2. Extraction and Purification of Humic Matter from Vermicompost
4.3. Humic Acids Characteristics
4.4. Plant Assay
4.5. Differential Transcription Level of Genes with RT-qPCR
4.6. Measurement of Trichome Density and Number
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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a DRIFT (cm−1) | |||||||||
2945 | 1660 | 1590 | 1460 | 1260 | 1100 | 1030 | |||
CH2 alkyl chains | COOH fatty acids | C=C aromatic units | CH2 alkyl chains | C-O alkyl acids/aromatic ehter | C-O phenols/polysaccahrides | C-O carbohydrates | |||
b 13C CPMAS NMR (ppm) | |||||||||
C=O 190-160 | O-aryl-C 160-145 | Aromatic-C 145-110 | O-alkyl-C 110-60 | CH3O/CN 60-45 | Alkyl-C 45-0 | HB | A/OA | Ar | LR |
6.5 | 8.4 | 20.3 | 28.3 | 16.7 | 19.8 | 1.3 | 0.7 | 28.7 | 2.0 |
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Silva, R.M.; Peres, A.N.A.; Peres, L.E.P.; Olivares, F.L.; Sangi, S.; Canellas, N.A.; Spaccini, R.; Cangemi, S.; Canellas, L.P. Humic Substances Isolated from Recycled Biomass Trigger Jasmonic Acid Biosynthesis and Signalling. Plants 2023, 12, 3148. https://doi.org/10.3390/plants12173148
Silva RM, Peres ANA, Peres LEP, Olivares FL, Sangi S, Canellas NA, Spaccini R, Cangemi S, Canellas LP. Humic Substances Isolated from Recycled Biomass Trigger Jasmonic Acid Biosynthesis and Signalling. Plants. 2023; 12(17):3148. https://doi.org/10.3390/plants12173148
Chicago/Turabian StyleSilva, Rakiely M., Alice N. A. Peres, Lázaro E. P. Peres, Fábio L. Olivares, Sara Sangi, Natália A. Canellas, Riccardo Spaccini, Silvana Cangemi, and Luciano P. Canellas. 2023. "Humic Substances Isolated from Recycled Biomass Trigger Jasmonic Acid Biosynthesis and Signalling" Plants 12, no. 17: 3148. https://doi.org/10.3390/plants12173148