Genome-Wide Association Study of Seed Morphology Traits in Senegalese Sorghum Cultivars
Abstract
:1. Introduction
2. Results
2.1. Seed Morphologies
2.2. Correlations among the Seed Morphology-Related Traits
2.3. Correlations between the Seed Morphology-Related Traits and Anthracnose and Head Smut Resistance Responses in Sorghum
2.4. Population Structure and GWAS Analysis
3. Discussion
4. Materials and Methods
4.1. Seed Phenotypic Evaluation
4.2. Statistical Analysis
4.3. GWAS and Population Genomic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Largest Area Size (mm2) | Smallest Area Size (mm2) | ||
---|---|---|---|
Accession | Mean ± S.D. | Accession | Mean ± S.D. |
1. PI514404 | 19.26 ± 3.41 | 1. PI514293 | 6.67 ± 0.86 |
2. PI514287 | 17.03 ± 2.26 | 2. PI514308 | 6.94 ± 1.1 |
3. PI514367 | 16.10 ± 2.03 | 3. PI514310 | 7.11 ± 0.87 |
4. PI514464 | 15.88 ± 2.71 | 4. PI514388 | 7.56 ± 1.05 |
5. PI514458 | 15.84 ± 1.76 | 5. PI514474 | 7.71 ± 0.82 |
Longest perimeter (mm) | Shortest perimeter (mm) | ||
1. PI514404 | 17.09 ± 1.63 | 1. PI514388 | 10.78 ± 0.76 |
2. PI514287 | 16.23 ± 1.34 | 2. PI514293 | 10.79 ± 1.01 |
3. PI514367 | 15.73 ± 1.13 | 3. PI514474 | 10.92 ± 0.61 |
4. PI514288 | 15.7 ± 1.24 | 4. PI514308 | 10.99 ± 1.21 |
5. PI514283 | 15.65 ± 1.53 | 5. PI514449 | 11.07 ± 0.68 |
Longest length (mm) | Shortest length (mm) | ||
1. PI514404 | 5.29 ± 0.46 | 1. PI514308 | 3.67 ± 0.39 |
2. PI514429 | 5.11 ± 0.3 | 2. PI514310 | 3.72 ± 0.26 |
3. PI514464 | 5.09 ± 0.57 | 3. PI514434 | 3.74 ± 0.22 |
4. PI514458 | 5.09 ± 0.34 | 4. PI514293 | 3.76 ± 0.28 |
5. PI514478 | 5.08 ± 0.31 | 5. PI514388 | 3.78 ± 0.27 |
Longest width (mm) | Shortest width (mm) | ||
1. PI514404 | 4.9 ± 0.48 | 1. PI514293 | 2.49 ± 0.19 |
2. PI514287 | 4.67 ± 0.35 | 2. PI514308 | 2.62 ± 0.3 |
3. PI514367 | 4.51 ± 0.3 | 3. PI514310 | 2.67 ± 0.19 |
4. PI514288 | 4.49 ± 0.37 | 4. PI514388 | 2.72 ± 0.2 |
5. PI514458 | 4.27 ± 0.26 | 5. PI514325 | 2.74 ± 0.23 |
Highest LWR | Lowest LWR | ||
1. PI514293 | 1.51 ± 0.1 | 1. PI514288 | 1.07 ± 0.04 |
2. PI514325 | 1.48 ± 0.08 | 2. PI514283 | 1.08 ± 0.04 |
3. PI514471 | 1.47 ± 0.08 | 3. PI514285 | 1.08 ± 0.05 |
4. PI514462 | 1.45 ± 0.09 | 4. PI514404 | 1.08 ± 0.04 |
5. PI514446 | 1.43 ± 0.06 | 5. PI514367 | 1.09 ± 0.06 |
Highest circularity (0–1 scale) | Lowest circularity (0–1 scale) | ||
1. PI514425 | 0.83 ± 0.01 | 1. PI514282 | 0.67 ± 0.08 |
2. PI514440 | 0.83 ± 0.02 | 2. PI514292 | 0.67 ± 0.07 |
3. PI514420 | 0.83 ± 0.02 | 3. PI514294 | 0.68 ± 0.06 |
4. PI514434 | 0.83 ± 0.02 | 4. PI514297 | 0.69 ± 0.07 |
5. PI514409 | 0.83 ± 0.02 | 5. PI514296 | 0.69 ± 0.07 |
The longest distance between IS and CG (mm) | Shortest distance between IS and CG (mm) | ||
1. PI514288 | 0.33 ± 0.19 | PI514388 | 0.19 ± 0.11 |
2. PI514294 | 0.32 ± 0.17 | PI514398 | 0.19 ± 0.13 |
3. PI514292 | 0.32 ± 0.21 | PI514446 | 0.2 ± 0.11 |
4. PI514283 | 0.32 ± 0.17 | PI514438 | 0.2 ± 0.12 |
5. PI514341 | 0.32 ± 0.17 | PI514449 | 0.2 ± 0.12 |
Brightest (0–255 scale) | Darkest (0–255 scale) | ||
1. PI514419 | 231.38 ± 19.43 | 1. PI514471 | 107.55 ± 10.76 |
2. PI514334 | 228.78 ± 15.28 | 2. PI514323 | 119.34 ± 20.39 |
3. PI514446 | 228.05 ± 18.19 | 3. PI514462 | 120.22 ± 12.47 |
4. PI514361 | 223.18 ± 15.75 | 4. PI514293 | 123.81 ± 20.84 |
5. PI514458 | 222.77 ± 20.39 | 5. PI514466 | 126.28 ± 15.52 |
Area Size | Perimeter | Length | Width | LWR | Circularity | IS and CG | Brightness | |
---|---|---|---|---|---|---|---|---|
Area size (mm2) | 1 *** | 0.95 *** | 0.93 *** | 0.97 *** | −0.67 *** | 0.33 *** | 0.36 *** | 0.09 |
Perimeter (mm) | 0.95 *** | 1 *** | 0.91 *** | 0.95 *** | −0.66 *** | 0.03 | 0.53 *** | 0.08 |
Length (mm) | 0.93 *** | 0.91 *** | 1 *** | 0.83 *** | −0.39 *** | 0.27 ** | 0.36 *** | 0.10 |
Width (mm) | 0.97 *** | 0.95 *** | 0.83 *** | 1 *** | −0.83 *** | 0.23 * | 0.41 *** | 0.07 |
LWR | −0.67 *** | −0.66 *** | −0.39 *** | −0.83 *** | 1 *** | −0.13 | −0.30 *** | −0.07 |
Circularity | 0.33 *** | 0.03 | 0.27 ** | 0.23 * | −0.13 | 1 *** | −0.45 *** | 0.06 |
IS and CG | 0.36 *** | 0.53 *** | 0.36 *** | 0.41 *** | −0.30 *** | −0.45 *** | 1 *** | −0.01 |
Brightness | 0.09 | 0.08 | 0.10 | 0.07 | −0.07 | 0.06 | −0.01 | 1 *** |
Trait | Chr | Location | Candidate Gene and Function | Distance (bp) | SNP Base % | TASSEL p-Value | Mean Value/SNP |
---|---|---|---|---|---|---|---|
Area | 6 | 12,058,855 and one more SNP within 3 bp | Sobic.006G036400 Similar to OSJNBb0043H09.3 protein Ring zinc finger | 136,397 | A: 84% G: 16% | 0.000000028 | A: 12.65 G: 10.05 |
Perimeter | 6 | 39,973,848 | Sobic.006G056100 Protein of unknown function (DUF3,537) | 0 | A: 85.2% G: 14.8% | 0.000000074 | A: 14.11 G: 12.44 |
Perimeter | 2 | 36,673,863 | Sobic.002G147466 Ribonucleotide reductase-related | 0 | C: 92.9% G: 7.1% | 0.000000013 | C: 13.76 G: 14.7 |
Perimeter | 1 | 72,472,059 | Sobic.001G447400 Similar to homeobox-leucine zipper protein HAT22 | 2530 | A: 16.3% C: 83.7% | 0.000000033 | A: 12.06 C: 14.14 |
Perimeter | 2 | 46,916,695 | Sobic.002G154750 Tetratricopeptide repeat-containing protein (TPR) Associated PlantFAMs- Ankyrin-1 | 6908 | G: 16% T: 84% | 0.00000004 | G: 12.59 T: 14.09 |
Perimeter | 6 | 51,902,835 and one more within 26 bp | Sobic.006G161000 Calcium/calmodulin-dependent protein kinase kinase (CAMKK) | 2571 | A: 15.2% G: 84.8% | 0.000000043 | A: 12.05 G: 14.13 |
Perimeter | 2 | 46,926,845 | Sobic.002G154800 Similar to glycosyl transferase family 1 protein-like Mannosylfructose-phosphate synthase/MFPS | 0 | C: 16.6% G: 83.4% | 0.000000063 | C: 12.38 G: 14.09 |
Perimeter | 7 | 16,244,875 | Sobic.007G093100 No annotation Associated PlantFAMs-zinc-finger of the FCS-type | 37,632 | C: 83.2% T: 16.8% | 0.000000077 | C: 14.13 T: 12.06 |
Perimeter | 4 | 67,200,818 | Sobic.004G340100 PB1 domain | 2091 | A: 15.9% G: 84.1% | 0.00000011 | A: 12.03 G: 14.13 |
Perimeter | 2 | 36,482,136 | Sobic.002G147433 Zinc finger | 137,836 | A: 6.4% G: 93.6% | 0.00000012 | A: 14.71 G: 13.74 |
Perimeter | 2 | 36,533,975 | Sobic.002G147466 Ribonucleotide reductase-related | 139,787 | C: 93.5% T: 6.5% | 0.00000015 | C: 13.74 T: 14.71 |
Width | 6 | 12,058,855 and one more SNP within 3 bp | Sobic.006G036400 Similar to OSJNBb0043H09.3 protein Ring zinc finger | 136,397 | A: 84% G: 16% | 0.000000011 | A: 3.67 G: 3.2 |
Width | 6 | 37,776,989 | Sobic.006G050400 Protein of unknown function (DUF493) | 0 | A: 11.3% G: 88.7% | 0.00000002 | A: 2.99 G: 3.64 |
Width | 2 | 36,673,863 | Sobic.002G147466 Ribonucleotide reductase-related | 0 | C: 92.9% G: 7.1% | 0.000000068 | C: 3.56 G: 3.94 |
Width | 7 | 16,244,901 and one more SNP within 26 bp | Sobic.007G093100 No annotation Associated PlantFAMs- zinc-finger of the FCS-type | 37,606 | A: 16.8% T: 83.2% | 0.00000011 | A: 3.05 T: 3.68 |
Width | 1 | 52,374,442 | Sobic.001G271500 Leucine-rich repeat-containing protein | 22,206 | G: 90.6% T: 9.4% | 0.00000014 | G: 3.63 T: 2.99 |
PCs | 3 | 5,165,375 | Sobic.003G058600 Cytochrome P450 CYP2 subfamily | 0 | A: 7.5% G: 92.5% | 0.00000000044 | |
PCs | 6 | 58,756,099 | Sobic.006G248000 Farnesyl-pyrophosphate synthetase | 0 | C: 87.9% T: 12.1% | 0.0000000017 | |
PCs | 4 | 62,452,658 and one more SNP within 11 bp | Sobic.004G282700 Similar to DTDP-D-glucose 4,6-dehydratase-like | 2043 | G: 92.5 T: 7.5% | 0.0000000044 | |
PCs | 6 | 8,316,056 | Sobic.006G033200 Similar to Probable protein transport Sec1a Syntaxin-binding protein 1 (STXBP1, MUNC18-1) | 266,373 | G: 25.3% T: 74.7% | 0.0000000054 | |
PCs | 6 | 54,692,844 | Sobic.006G193600 Zinc-finger of the FCS-type, C2-C2 (zf-FLZ) | 214 | C: 94.2% G: 5.8% | 0.0000000071 | |
PCs | 6 | 54,708,681 and one more SNP within 11 bp | Sobic.006G193700 Zinc-finger of the FCS-type, C2-C2 (zf-FLZ) | 0 | G: 94.2% T: 5.8% | 0.0000000071 | |
PCs | 3 | 5,151,221 | Sobic.003G058500 Golgi to er traffic protein 4 homolog | 0 | C: 92.5% T: 7.5% | 0.0000000075 | |
PCs | 4 | 62,432,641 | Sobic.004G282400 Rho guanosine triphosphatases (GTPase) activating protein 2 | 0 | C: 93.1 T: 6.9% | 0.0000000095 | |
PCs | 4 | 62,438,345 | Sobic.004G282500 Pentatricopeptide (PPR) repeat-containing protein-like | 11 | C: 93.1 T: 6.9% | 0.0000000095 | |
PCs | 3 | 51,995,650 | Sobic.003G195900 Stomatal cytokinesis defective Associated PlantFAMs- WD40 repeat-containing protein | 0 | A: 5.7% G: 94.3 | 0.000000012 | |
Brightness | 10 | 15,656,073 | Sobic.010G126000 WD-40 repeat protein | 17,962 | C: 92.5% T: 7.5% | 0.0000000093 | C: 198.62 T: 156.23 |
Brightness | 1 | 53,382,065 | Sobic.001G275300 ATP-dependent DNA helicase | 11,647 | G: 7.7% T: 92.3% | 0.000000019 | G: 186.61 T: 198.24 |
Brightness | 10 | 53,260,179 | Sobic.010G191301 No annotation Highly expressed in panicle-upper anthesis | 7571 | C: 7% G: 93% | 0.000000035 | C: 177.47 G: 197.87 |
Brightness | 1 | 2,657,706 | Sobic.001G035200 Amyloid beta precursor protein-binding protein 1 (APPBP1) | 0 | A: 92.4% G: 7.6% | 0.000000058 | A: 198.38 G: 156.23 |
Brightness | 1 | 66,050,539 | Sobic.001G372400 No annotation Associated PlantFAMs- Ribonuclease III | 806 | C: 94.3% T: 5.7% | 0.00000015 | C: 198.3 T: 155.85 |
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Ahn, E.; Botkin, J.; Ellur, V.; Lee, Y.; Poudel, K.; Prom, L.K.; Magill, C. Genome-Wide Association Study of Seed Morphology Traits in Senegalese Sorghum Cultivars. Plants 2023, 12, 2344. https://doi.org/10.3390/plants12122344
Ahn E, Botkin J, Ellur V, Lee Y, Poudel K, Prom LK, Magill C. Genome-Wide Association Study of Seed Morphology Traits in Senegalese Sorghum Cultivars. Plants. 2023; 12(12):2344. https://doi.org/10.3390/plants12122344
Chicago/Turabian StyleAhn, Ezekiel, Jacob Botkin, Vishnutej Ellur, Yoonjung Lee, Kabita Poudel, Louis K. Prom, and Clint Magill. 2023. "Genome-Wide Association Study of Seed Morphology Traits in Senegalese Sorghum Cultivars" Plants 12, no. 12: 2344. https://doi.org/10.3390/plants12122344