Mapping and Validation of qHD7b: Major Heading-Date QTL Functions Mainly under Long-Day Conditions
Abstract
:1. Introduction
2. Results
2.1. Phenotypic and Genotypic Analysis of CSSLs
2.2. QTL Analysis of the CSSLs
2.3. Photoperiodic Response of qHD7b
2.4. QTL Mapping in BC5F2:3 Population
2.5. Fine Mapping of qHD7b
2.6. Candidate Gene Analysis of qHD7b and Validation Using CRISPR/Cas9
3. Discussion
4. Materials and Methods
4.1. Population Development and Phenotyping
4.2. Test of Day Length Response in Growth Chambers
4.3. Molecular Markers Development and DNA Extraction
4.4. RNA Extraction and qRT-PCR
4.5. Generation of ghd7 Mutant Using CRISPR/Cas9 System
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parents b | 76 CSSLs a | ||||||||
---|---|---|---|---|---|---|---|---|---|
Year/Location | ZH9308 | XQZB | Min | Max | Mean | SD | Kurtosis | Skewness | H |
2014 Hangzhou | 91.83 ± 1.11 ** | 60.17 ± 1.10 | 60.40 | 119.22 | 87.12 | 8.89 | 5.21 | −0.23 | 0.79 |
2015 Hangzhou | 88.33 ± 0.50 ** | 56.67 ± 0.54 | 57.67 | 124.28 | 88.21 | 9.42 | 4.45 | −0.33 | |
2015 Hainan | 110.99 ± 1.52 ** | 91.00 ± 1.31 | 96.57 | 124.94 | 110.87 | 3.94 | 4.33 | 0.63 | 0.82 |
2016 Hainan | 109.67 ± 1.24 ** | 91.44 ± 1.67 | 90.00 | 122.80 | 103.59 | 6.10 | 1.54 | 0.36 | |
Combined | |||||||||
Trait | Min | Max | Mean | σ2G | σ2GxE | σ2E | CV | H2 | |
HD | 80.77 | 119.78 | 97.45 | 34.86 | 24.91 | 8.98 | 3.08 | 0.83 |
QTLs | Year/Location a | Chr. | Region (cM) | Flanking Markers | LOD b | PVE (%) c | Add d |
---|---|---|---|---|---|---|---|
qHD2a | 2015HZ | 2 | 19.77 | RM6424-InD31 | 2.80 | 2.54 | 2.37 |
qHD4a | 2014HZ | 4 | 6.58 | InD62-RM1205 | 38.89 | 9.87 | 13.13 |
2015HZ | 4 | 10.54 | RM1205-RM5979 | 6.46 | 4.50 | 1.75 | |
qHD4b | 2015HZ | 4 | 21.08 | RM3839-RM241 | 11.33 | 9.14 | 8.79 |
qHD5a | 2014HZ | 5 | 25.20 | RM3638-RM6841 | 2.59 | 0.45 | −0.47 |
2016HN | 5 | 14.52 | InD79-RM3638 | 2.91 | 7.78 | −3.91 | |
qHD6a | 2014HZ | 6 | 6.58 | RM5754-RM5963 | 49.67 | 19.03 | 18.21 |
2015HN | 6 | 5.27 | RM510-RM5754 | 11.12 | 36.14 | 6.78 | |
2015HZ | 6 | 6.58 | RM5754-RM5963 | 13.14 | 10.13 | 9.90 | |
2016HN | 6 | 5.27 | RM510-RM5754 | 8.83 | 25.01 | 8.67 | |
qHD6b | 2014HZ | 6 | 11.85 | RM20069-InD94 | 3.77 | 0.33 | 0.36 |
qHD7b | 2014HZ | 7 | 27.69 | RM3859-RM5875 | 54.42 | 26.54 | −15.44 |
2015HN | 7 | 27.69 | RM3859-RM5875 | 3.24 | 8.10 | −2.61 | |
2015HZ | 7 | 27.69 | RM3859-RM5875 | 26.56 | 32.84 | −12.90 | |
2016HN | 7 | 27.69 | RM3859-RM5875 | 7.55 | 20.33 | −6.76 | |
qHD7c | 2015HZ | 7 | 21.13 | RM1132-RM455 | 2.56 | 2.79 | 0.88 |
qHD8a | 2014HZ | 8 | 7.91 | RM5556-RM22529 | 46.40 | 16.12 | −12.03 |
2015HZ | 8 | 7.91 | RM5556-RM22529 | 15.91 | 13.30 | −8.22 | |
qHD10a | 2015HZ | 10 | 2.63 | InD133-InD135 | 12.27 | 9.14 | −13.28 |
2015HN | 10 | 2.63 | InD133-InD135 | 6.67 | 18.74 | −7.37 | |
qHD10b | 2016HN | 10 | 6.58 | RM6142-RM5620 | 3.60 | 8.89 | −5.96 |
qHD11a | 2014HZ | 11 | 10.54 | RM7463-RM26652 | 18.14 | 1.94 | −7.76 |
2015HZ | 11 | 17.16 | RM26652-InD151 | 2.51 | 3.10 | −2.72 | |
qHD12a | 2014HZ | 12 | 2.63 | InD156-RM7003 | 15.42 | 2.21 | −6.10 |
qHD12b | 2014HZ | 12 | 23.82 | InD165-RM1300 | 2.85 | 0.43 | −0.44 |
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Sohail, A.; Shah, L.; Liu, L.; Islam, A.; Yang, Z.; Yang, Q.; Anis, G.B.; Xu, P.; Khan, R.M.; Li, J.; et al. Mapping and Validation of qHD7b: Major Heading-Date QTL Functions Mainly under Long-Day Conditions. Plants 2022, 11, 2288. https://doi.org/10.3390/plants11172288
Sohail A, Shah L, Liu L, Islam A, Yang Z, Yang Q, Anis GB, Xu P, Khan RM, Li J, et al. Mapping and Validation of qHD7b: Major Heading-Date QTL Functions Mainly under Long-Day Conditions. Plants. 2022; 11(17):2288. https://doi.org/10.3390/plants11172288
Chicago/Turabian StyleSohail, Amir, Liaqat Shah, Ling Liu, Anowerul Islam, Zhengfu Yang, Qinqin Yang, Galal Bakr Anis, Peng Xu, Riaz Muhammad Khan, Jiaxin Li, and et al. 2022. "Mapping and Validation of qHD7b: Major Heading-Date QTL Functions Mainly under Long-Day Conditions" Plants 11, no. 17: 2288. https://doi.org/10.3390/plants11172288