Improving the Phenolic Content of Tempranillo Grapes by Sustainable Strategies in the Vineyard
Abstract
:1. Introduction
2. Results and Discussion
2.1. Grape Skin Total Phenolic Composition
2.2. Grape Skin Individual Phenolic Composition
2.2.1. Anthocyanin Profile
2.2.2. Flavonol Profile
2.2.3. Flavanols
2.2.4. Hydroxycinnamic Compounds and Stilbene
2.2.5. Principal Component Analysis (PCA)
3. Materials and Methods
3.1. Plant Material
3.2. Experimental Design
3.3. Climate Conditions
3.4. Leaf Area
3.5. Phenolic Compounds Analysis
3.6. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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DpG | CyG | PtG | PnG | MvG | ∑G | DpA | CyA | PtA | PnA | MvA | ∑Ac | DpC | CyC | PtC | PnC | MvC | ∑Cm | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2009 | ||||||||||||||||||
T | 85.5 ab | 9.9 | 85.4 ab | 18.4 | 266.4 ab | 476.9 ab | 4.8 a | 1.1 | 6.5 a | 0.9 | 26.6 a | 40.5 a | 3.2 a | 3.8 | 39.0 | 7.4 | 142.8 | 201.1 |
EDI | 96.4 a | 13.4 | 92.4 a | 22.8 | 304.4 a | 542.1 a | 4.8 a | 1.2 | 6.2 a | 0.9 | 27.0 a | 40.8 a | 2.9 ab | 4.0 | 38.2 | 7.6 | 152.8 | 210.5 |
LDI | 75.3 b | 11.2 | 71.7 b | 18.3 | 230.7 b | 417.0 b | 3.8 b | 1.0 | 4.8 b | 0.7 | 22.0 b | 32.9 b | 1.6 b | 3.5 | 33.1 | 6.8 | 132.8 | 182.2 |
C | 83.4 | 11.2 | 81.4 | 20.1 | 268.4 | 475.6 | 4.1 b | 1.0 | 5.4 b | 0.8 | 24.3 | 36.2 | 2.6 | 3.5 | 35.8 | 7.2 | 145.3 | 199.2 |
TH | 88.1 | 11.8 | 85.0 | 19.6 | 266.0 | 481.8 | 4.8 a | 1.2 | 6.3 a | 0.9 | 26.1 | 39.9 | 2.6 | 4.0 | 37.7 | 7.4 | 140.2 | 196.7 |
T-C | 87.1 | 11.5 | 87.1 ab | 21.0 | 273.7 ab | 492.0 ab | 4.6 ab | 1.1 | 6.3 ab | 1.0 | 27.4 | 41.1 ab | 3.9 | 3.8 | 40.4 | 7.7 | 151.4 ab | 212.2 |
T-TH | 83.8 | 8.3 | 83.7 ab | 15.9 | 259.2 ab | 461.8 ab | 5.0 a | 1.1 | 6.6 a | 0.8 | 25.8 | 39.9 ab | 2.6 | 3.9 | 37.6 | 7.1 | 134.3 ab | 190.1 |
EDI-C | 98.4 | 13.3 | 92.6 a | 23.3 | 308.7 a | 549.2 a | 4.6 ab | 1.1 | 5.6 ab | 0.8 | 24.7 | 37.3 ab | 2.0 | 3.7 | 35.3 | 7.6 | 143.3 ab | 196.6 |
EDI-TH | 94.4 | 13.5 | 92.2 a | 22.2 | 300.0 a | 535.1 a | 5.1 a | 1.2 | 6.8 a | 1.1 | 29.4 | 44.4 a | 3.7 | 4.3 | 41.0 | 7.6 | 162.3 a | 224.3 |
LDI-C | 64.6 | 8.8 | 64.3 b | 15.9 | 222.7 b | 385.6 b | 3.0 b | 0.9 | 4.2 b | 0.5 | 21.0 | 30.2 b | 1.8 | 3.1 | 31.7 | 6.2 | 141.4 ab | 188.8 |
LDI-TH | 86.0 | 13.5 | 79.1 ab | 20.7 | 238.7 ab | 448.5 ab | 4.5 ab | 1.2 | 5.4 ab | 1.0 | 23.0 | 35.6 ab | 1.4 | 3.9 | 34.5 | 7.4 | 124.2 b | 175.7 |
Significance | ||||||||||||||||||
RDI | * | ns | * | ns | ** | ** | * | ns | ** | ns | * | * | * | ns | ns | ns | ns | ns |
CL | ns | ns | ns | ns | ns | ns | * | ns | * | ns | ns | ns | ns | ns | ns | ns | ns | ns |
RDI*CL | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns |
Treatment | ns | ns | * | ns | * | * | * | ns | * | ns | ns | * | ns | ns | ns | ns | * | ns |
Dp | Cy | Pt | Pn | Mv | ∑G | DpA | CyA | PtA | PnA | MvA | ∑Ac | DpC | CyC | PtC | PnC | MvC | ∑Cm | |
2010 | ||||||||||||||||||
T | 69.9 | 8.3 a | 70.8 | 27.0 a | 273.7 a | 460.5 a | 5.1 a | 1.1 | 7.6 a | 1.7 a | 38.8 | 55.1 a | 1.2 | 4.5 a | 49.2 | 9.5 a | 219.4 | 290.9 |
EDI | 52.8 | 4.5 b | 61.1 | 19.0 ab | 233.9 b | 380.3 b | 4.3 b | 1.0 | 6.7 b | 1.0 b | 35.9 | 49.7 b | 1.2 | 3.7 ab | 45.1 | 8.2 ab | 203.5 | 268.2 |
LDI | 58.6 | 6.8 ab | 65.7 | 17.0 b | 251.7 ab | 409.6 ab | 4.3 b | 1.0 | 6.5 b | 0.9 b | 35.7 | 49.2 b | 1.0 | 3.5 b | 45.6 | 7.7 b | 206.2 | 270.4 |
C | 49.8 b | 5.0 b | 53.0 | 19.1 | 235.3 b | 371.0 b | 4.0 b | 0.9 | 6.6 b | 1.1 | 36.1 | 49.6 b | 1.1 | 3.4 b | 44.9 | 7.8 b | 211.9 | 275.7 |
TH | 71.0 a | 8.1 a | 78.8 | 22.9 | 270.9 a | 462.6 a | 5.1 a | 1.2 | 7.2 a | 1.3 | 37.5 | 53.1 a | 1.2 | 4.4 a | 48.3 | 9.1 a | 207.5 | 277.3 |
T-C | 57.6 | 6.0 bc | 59.4 ab | 25.1 | 259.2 ab | 417.1 abc | 4.5 ab | 0.9 | 7.3 a | 1.7 a | 38.7 | 54.0 ab | 1.1 | 4.3 ab | 48.7 | 9.4 a | 223.4 | 294.0 |
T-TH | 82.2 | 10.5 a | 82.2 a | 28.9 | 288.2 a | 503.8 a | 5.7 a | 1.3 | 7.8 a | 1.7 a | 38.8 | 56.2 a | 1.3 | 4.8 a | 49.7 | 9.6 a | 215.5 | 287.7 |
EDI-C | 43.7 | 3.9 c | 48.6 b | 16.3 | 207.2 b | 327.5 c | 3.7 b | 0.9 | 6.5 ab | 0.8 c | 35.1 | 47.8 ab | 1.1 | 3.0 b | 43.2 | 7.1 b | 205.9 | 266.6 |
EDI-TH | 61.8 | 5.2 bc | 73.5 ab | 21.7 | 260.6 ab | 433.1 abc | 4.9 ab | 1.1 | 6.9 ab | 1.2 abc | 36.7 | 51.6 ab | 1.2 | 4.5 a | 47.1 | 9.4 a | 201.1 | 269.8 |
LDI-C | 48.1 | 5.1 bc | 50.9 b | 16.0 | 239.4 ab | 368.3 bc | 3.9 b | 0.9 | 6.0 b | 0.8 bc | 34.4 | 46.9 b | 1.0 | 2.9 b | 42.9 | 6.9 b | 206.4 | 266.5 |
LDI-TH | 69.2 | 8.5 ab | 80.6 ab | 18.0 | 263.9 ab | 450.8 ab | 4.7 ab | 1.1 | 6.9 ab | 1.0 abc | 37.1 | 51.6 ab | 1.0 | 4.0 ab | 48.3 | 8.4 ab | 206.0 | 274.3 |
Significance | ||||||||||||||||||
RDI | ns | *** | ns | * | * | * | * | ns | ** | ** | ns | * | ns | ** | ns | * | ns | ns |
CL | * | *** | *** | ns | ** | *** | ** | ns | * | ns | ns | * | ns | ** | ns | * | ns | ns |
RDI*CL | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns |
Treatment | ns | *** | *** | ns | * | ** | ** | ns | ** | ** | ns | * | ns | ** | ns | * | ns | ns |
My | Qc | Kp | Ih | |
---|---|---|---|---|
2009 | ||||
T | 30.3 | 10.3 a | 2.6 a | 4.6 a |
EDI | 24.7 | 8.6 b | 1.6 b | 3.8 b |
LDI | 26.3 | 8.3 b | 1.9 ab | 3.3 b |
C | 29.2 a | 9.0 | 1.8 | 4.0 |
TH | 25.0 b | 9.2 | 2.2 | 3.8 |
T-C | 33.7 | 9.7 ab | 2.1 ab | 4.8 a |
T-TH | 26.9 | 11.0 a | 3.0 ab | 4.3 ab |
EDI-C | 27.7 | 9.2 ab | 1.5 b | 4.2 ab |
EDI-TH | 21.8 | 8.0 b | 1.7 ab | 3.5 bc |
LDI-C | 26.2 | 8.1 b | 1.7 ab | 3.1 c |
LDI-TH | 26.4 | 8.6 ab | 2.0 ab | 3.6 bc |
Significance | ||||
RDI | ns | ** | * | *** |
CL | * | ns | ns | ns |
RDI*Cl | ns | ns | ns | * |
Treatment | ns | * | * | ** |
2010 | ||||
T | 24.4 a | 9.4 | 1.3 | 4.9 a |
EDI | 19.7 b | 8.7 | 1.2 | 4.3 b |
LDI | 22.8 ab | 9.3 | 1.6 | 4.8 ab |
C | 17.6 b | 7.5 b | 1.2 b | 4.2 b |
TH | 26.9 a | 10.8 a | 1.6 a | 5.1 a |
T-C | 19.8 b | 9.2 | 1.2 ab | 4.6 ab |
T-TH | 28.9 a | 9.6 | 1.4 ab | 5.2 a |
EDI-C | 16.6 b | 7.9 | 1.2 ab | 4.0 b |
EDI-TH | 22.8 ab | 9.4 | 1.2 ab | 4.6 ab |
LDI-C | 16.5 b | 5.3 | 1.1 b | 4.1 b |
LDI-TH | 29.1 a | 13.4 | 2.1 a | 5.5 a |
Significance | ||||
RDI | * | ns | ns | * |
CL | *** | * | * | *** |
RDI*CL | ns | ns | ns | ns |
Treatment | *** | ns | * | *** |
CAT | EPI | PB1 | PB2 | PB3 | |
---|---|---|---|---|---|
2009 | |||||
T | 1.43 b | 0.03 b | 1.37 c | 0.44 b | 2.96 b |
EDI | 1.87 ab | 0.04 b | 2.11 b | 0.60 ab | 3.53 ab |
LDI | 2.26 a | 0.26 a | 2.63 a | 0.80 a | 3.95 a |
C | 1.74 | 0.06 b | 1.90 b | 0.67 | 3.66 |
TH | 1.97 | 0.16 a | 2.18 a | 0.56 | 3.30 |
T-C | 1.44 b | 0.04 b | 1.27 c | 0.52 ab | 3.26 ab |
T-TH | 1.41 b | 0.03 b | 1.47 c | 0.37 b | 2.66 b |
EDI-C | 1.75 ab | 0.00 b | 1.82 bc | 0.66 ab | 3.66 ab |
EDI-TH | 1.99 ab | 0.08 b | 2.40 ab | 0.53 ab | 3.40 ab |
LDI-C | 2.03 ab | 0.13 b | 2.60 a | 0.82 a | 4.06 a |
LDI-TH | 2.50 a | 0.38 a | 2.65 a | 0.79 a | 3.84 a |
Significance | |||||
RDI | ** | *** | *** | ** | * |
CL | ns | * | * | ns | ns |
RDI*CL | ns | * | ns | ns | ns |
Treatment | ** | *** | *** | ** | ns |
2010 | |||||
T | 1.54 | 0.41 | 3.25 | 0.41 | 3.46 ab |
EDI | 1.61 | 0.49 | 3.19 | 0.44 | 3.26 b |
LDI | 1.86 | 0.57 | 3.46 | 0.37 | 4.14 a |
C | 1.49 | 0.49 | 2.57 b | 0.38 | 3.11 b |
TH | 1.85 | 0.49 | 4.03 a | 0.43 | 4.14 a |
T-C | 1.34 | 0.40 | 2.47 b | 0.39 | 3.46 b |
T-TH | 1.74 | 0.42 | 4.03 a | 0.42 | 3.47 ab |
EDI-C | 1.36 | 0.53 | 2.53 b | 0.36 | 2.53 b |
EDI-TH | 1.86 | 0.45 | 3.84 a | 0.52 | 4.00 ab |
LDI-C | 1.77 | 0.55 | 2.71 b | 0.40 | 3.34 b |
LDI-TH | 1.96 | 0.59 | 4.22 a | 0.34 | 4.95 a |
Significance | |||||
RDI | ns | ns | ns | ns | * |
CL | ns | ns | *** | ns | ** |
RDI*CL | ns | ns | ns | ns | * |
Treatment | ns | ns | * | ns | ** |
CF | COU | FE | CF-T | COU-T | FE-T | Trans-R | |
---|---|---|---|---|---|---|---|
2009 | |||||||
T | 1.2 ab | 3.0 a | 0.7 a | 2.8 a | 7.9 b | 0.7 ab | 0.08 b |
EDI | 1.3 a | 2.6 ab | 0.4 b | 3.1 a | 12.7 a | 0.9 a | 0.18 a |
LDI | 0.9 b | 2.2 b | 0.5 ab | 1.9 b | 7.2 b | 0.6 b | 0.10 b |
C | 1.1 | 2.6 | 0.5 | 2.3 b | 10.7 a | 0.8 | 0.11 |
TH | 1.2 | 2.6 | 0.6 | 2.9 a | 7.8 b | 0.6 | 0.13 |
T-C | 1.1 | 3.0 | 0.8 a | 2.6 a | 8.1 b | 0.7 ab | 0.10 b |
T-TH | 1.2 | 3.0 | 0.7 ab | 3.0 a | 7.8 b | 0.6 ab | 0.07 b |
EDI-C | 1.2 | 2.8 | 0.3 b | 3.0 a | 16.6 a | 1.0 a | 0.13 b |
EDI-TH | 1.3 | 2.5 | 0.5 ab | 3.2 a | 8.8 b | 0.7 ab | 0.22 a |
LDI-C | 0.9 | 1.9 | 0.4 ab | 1.4 b | 7.5 b | 0.6 b | 0.10 b |
LDI-TH | 1.0 | 2.5 | 0.5 ab | 2.5 a | 7.0 b | 0.6 b | 0.10 b |
Significance | |||||||
RDI | * | * | * | *** | *** | ** | *** |
CL | ns | ns | ns | ** | *** | ns | ns |
RDI*CL | ns | ns | ns | ns | *** | ns | ** |
Treatment | ns | ns | * | ** | *** | * | *** |
2010 | |||||||
T | 0.8 b | 1.9 b | 0.5 b | 2.2 b | 4.7 b | 0.2 | 0.06 b |
EDI | 1.6 a | 6.0 a | 1.5 a | 3.0 a | 9.2 a | 0.2 | 0.11 a |
LDI | 0.7 b | 6.0 a | 1.2 ab | 1.7 b | 2.8 c | 0.2 | 0.07 b |
C | 0.6 b | 4.4 | 1.1 | 1.9 b | 3.4 b | 0.2 | 0.09 |
TH | 1.4 a | 4.9 | 1.0 | 2.9 a | 7.7 a | 0.2 | 0.07 |
T-C | 0.5 d | 1.4 b | 0.4 b | 1.6 c | 3.4 bc | 0.2 | 0.05 |
T-TH | 1.2 b | 2.5 b | 0.6 b | 2.9 ab | 5.9 b | 0.3 | 0.07 |
EDI-C | 1.0 bc | 3.3 b | 1.1 b | 2.4 bc | 4.9 b | 0.3 | 0.11 |
EDI-TH | 2.3 a | 8.7 a | 1.9 a | 3.7 a | 13.5 a | 0.2 | 0.10 |
LDI-C | 0.5 cd | 8.5 a | 1.8 a | 1.7 c | 2.0 c | 0.2 | 0.09 |
LDI-TH | 0.8 bcd | 3.5 b | 0.7 b | 2.1 bc | 3.5 bc | 0.2 | 0.05 |
Significance | |||||||
RDI | *** | *** | * | ** | *** | ns | * |
CL | *** | ns | ns | *** | *** | ns | ns |
RDI*CL | ** | *** | ns | ns | *** | ns | ns |
Treatment | *** | *** | * | *** | *** | ns | * |
Meteorological Data | 2009 | 2010 | |
---|---|---|---|
Mean Temperature (°C) | Pre-veraison 1 | 19 | 19 |
Post-veraison 2 | 25 | 26 | |
Growing season 3 | 22 | 23 | |
Rainfall (mm) | Pre-veraison | 85 | 137 |
Post-veraison | 6 | 5 | |
Growing season | 91 | 142 | |
Annual 4 | 402 | 734 | |
Irrigation (mm) | |||
EDI | Total | 332 | 207 |
LDI | Total | 274 | 129 |
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Valdés, M.E.; Talaverano, M.I.; Moreno, D.; Uriarte, D.; Mancha, L.; Vilanova, M. Improving the Phenolic Content of Tempranillo Grapes by Sustainable Strategies in the Vineyard. Plants 2022, 11, 1393. https://doi.org/10.3390/plants11111393
Valdés ME, Talaverano MI, Moreno D, Uriarte D, Mancha L, Vilanova M. Improving the Phenolic Content of Tempranillo Grapes by Sustainable Strategies in the Vineyard. Plants. 2022; 11(11):1393. https://doi.org/10.3390/plants11111393
Chicago/Turabian StyleValdés, M. Esperanza, M. Inmaculada Talaverano, Daniel Moreno, David Uriarte, Luis Mancha, and Mar Vilanova. 2022. "Improving the Phenolic Content of Tempranillo Grapes by Sustainable Strategies in the Vineyard" Plants 11, no. 11: 1393. https://doi.org/10.3390/plants11111393