Zinc Modulation of Neuronal Calcium Sensor Proteins: Three Modes of Interaction with Different Structural Outcomes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of NCS Proteins
2.2. Isothermal Titration Calorimetry (ITC)
2.3. Nano Differential Scanning Fluorimetry (nanoDSF)
2.4. Thermal Shift Assay
2.5. Dynamic Light Scattering (DLS)
2.6. Structural Bioinformatics Predictions
2.7. Data Analysis and Visualization
3. Results
3.1. Coordination of Zn2+ by NCS Proteins
3.2. Zn2+-Induced Conformational Changes in NCS Proteins
3.3. Thermal Stability of Zn2+-bound NCS Proteins
3.4. Zn2+-Dependent Aggregation and Oligomerization of NCS Proteins
3.5. Potential Zn2+-Binding Sites of NCS Proteins
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Protein | Ca2+ | ITC | TS (or nanoDSF) | DLS | ||
---|---|---|---|---|---|---|
N * | KD, μM | ΔH *, kcal/mol | Tm *, °C (−/+ Zn2+) | Dh *, nm (−/+ Zn2+) | ||
recoverin | − | 1.8 | (1.0 ± 0.2) × 10−6 | −11.4 | 50.3/54.8 | 4.6/5.7 |
+ | 1.3 | (2.7 ± 0.3) × 10−6 | 3.0 | 78.4/69.3 | 4.6/4.7 | |
VILIP1 | − | 1.3 0.6 | (4.3 ± 1.0) × 10−8, (1.7 ± 0.2) × 10−6 | −2.7 −1.8 | 68.6/63.7; 38.1 | 6.3/6.6 |
+ | no binding | n.d./38.2 | 6.7/6.9 | |||
NCLD | − | 0.9 | (2.6 ± 0.5) × 10−5 | −2.4 | 61.1/38.8 | 5.4/5.8 |
+ | 2.6 | (2.0 ± 0.5) × 10−5 | −1.4 | 84.5/51.2 | 5.8/6.6 | |
GCAP1 | − | 0.7 | (1.4 ± 0.2) × 10−5 | −9.3 | n.d. | 5.9/6.8 |
+ | 1.1 | (1.1 ± 0.1) × 10−5 | −6.1 | n.d. | 5.9/7.3 | |
GCAP2 | − | n.d. | n.d. | n.d. | n.d. | 6.1/6.4 |
+ | n.d. | n.d. | n.d. | n.d. | 6.1/6.4 |
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Baksheeva, V.E.; Tsvetkov, P.O.; Zalevsky, A.O.; Vladimirov, V.I.; Gorokhovets, N.V.; Zinchenko, D.V.; Permyakov, S.E.; Devred, F.; Zernii, E.Y. Zinc Modulation of Neuronal Calcium Sensor Proteins: Three Modes of Interaction with Different Structural Outcomes. Biomolecules 2022, 12, 956. https://doi.org/10.3390/biom12070956
Baksheeva VE, Tsvetkov PO, Zalevsky AO, Vladimirov VI, Gorokhovets NV, Zinchenko DV, Permyakov SE, Devred F, Zernii EY. Zinc Modulation of Neuronal Calcium Sensor Proteins: Three Modes of Interaction with Different Structural Outcomes. Biomolecules. 2022; 12(7):956. https://doi.org/10.3390/biom12070956
Chicago/Turabian StyleBaksheeva, Viktoriia E., Philipp O. Tsvetkov, Arthur O. Zalevsky, Vasiliy I. Vladimirov, Neonila V. Gorokhovets, Dmitry V. Zinchenko, Sergei E. Permyakov, François Devred, and Evgeni Yu. Zernii. 2022. "Zinc Modulation of Neuronal Calcium Sensor Proteins: Three Modes of Interaction with Different Structural Outcomes" Biomolecules 12, no. 7: 956. https://doi.org/10.3390/biom12070956