Next Article in Journal
The Novel Protease Activities of JMJD5–JMJD6–JMJD7 and Arginine Methylation Activities of Arginine Methyltransferases Are Likely Coupled
Next Article in Special Issue
Gamma-Linolenic Acid (GLA) Protects against Ionizing Radiation-Induced Damage: An In Vitro and In Vivo Study
Previous Article in Journal
The Role of Lipids in CRAC Channel Function
Previous Article in Special Issue
Single-Cellular Biological Effects of Cholesterol-Catabolic Bile Acid-Based Nano/Micro Capsules as Anti-Inflammatory Cell Protective Systems
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 12
Issue 3
10.3390/biom12030353
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Utility of the Specialized Pro-Resolving Mediators as Diagnostic and Prognostic Biomarkers in Disease

by
Jesmond Dalli
1,2,*,
Esteban Alberto Gomez
1 and
Charlotte Camille Jouvene
1
1
William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK
2
Centre for Inflammation and Therapeutic Innovation, Queen Mary University of London, London EC1M 6BQ, UK
*
Author to whom correspondence should be addressed.
Biomolecules 2022, 12(3), 353; https://doi.org/10.3390/biom12030353
Submission received: 10 December 2021 / Revised: 18 February 2022 / Accepted: 19 February 2022 / Published: 23 February 2022
(This article belongs to the Collection Feature Papers in Biomacromolecules: Lipids)
Browse Figures
Review Reports Versions Notes

Abstract

:
A precision medicine approach is widely acknowledged to yield more effective therapeutic strategies in the treatment of patients with chronic inflammatory conditions than the prescriptive paradigm currently utilized in the management and treatment of these patients. This is because such an approach will take into consideration relevant factors including the likelihood that a patient will respond to given therapeutics based on their disease phenotype. Unfortunately, the application of this precision medicine paradigm in the daily treatment of patients has been greatly hampered by the lack of robust biomarkers, in particular biomarkers for determining early treatment responsiveness. Lipid mediators are central in the regulation of host immune responses during both the initiation and resolution of inflammation. Amongst lipid mediators, the specialized pro-resolving mediators (SPM) govern immune cells to promote the resolution of inflammation. These autacoids are produced via the stereoselective conversion of essential fatty acids to yield molecules that are dynamically regulated during inflammation and exert potent immunoregulatory activities. Furthermore, there is an increasing appreciation for the role that these mediators play in conveying the biological actions of several anti-inflammatory therapeutics, including statins and aspirin. Identification and quantitation of these mediators has traditionally been achieved using hyphenated mass spectrometric techniques, primarily liquid-chromatography tandem mass spectrometry. Recent advances in the field of chromatography and mass spectrometry have increased both the robustness and the sensitivity of this approach and its potential deployment for routine clinical diagnostics. In the present review, we explore the evidence supporting a role for specific SPM as potential biomarkers for patient stratification in distinct disease settings together with methodologies employed in the identification and quantitation of these autacoids.

1. Introduction

Recent years have seen an increased appreciation for the need of developing a more personalized approach to the treatment of patients with inflammatory conditions [1,2]. Indeed, it is now abundantly apparent that the paradigm where patients are treated using a broad and formulaic approach does not best serve them. This is because such an approach leads to delays in their treatment with effective therapeutics, thus allowing the condition to precipitate. In addition, the administration of ineffective drugs also unnecessarily exposes these patients to their unwanted side effects [1,2]. Such considerations are especially important in conditions where there is a progressive destructive inflammation, such as, for example, in patients with rheumatoid arthritis (RA) treated with disease modifying anti-rheumatic drugs, where up to 50% of patients will not respond to these therapeutics [3,4]. Several aspects have hampered the development of patient tailored therapeutics, including a limited understanding of the molecular and cellular mechanisms that contribute to the disease as well as the availability of robust biomarkers that are effective at predicting treatment responses or sufficiently sensitive for evaluating treatment responsiveness early in the treatment regime.
Prostaglandins, thromboxanes and leukotrienes are initiators of the inflammatory response promoting vascular leak, leukocyte recruitment, thrombosis and smooth muscle contraction. Lipoxins, resolvins, protectins and maresins are agonists of resolution. They counter-regulate the formation and actions of inflammation-initiators and promote the clearance of apoptotic cells, the uptake and killing of bacteria by phagocytes and tissue repair and regeneration.
Studies conducted in the early 2000s investigating mechanisms engaged by the immune system to regulate innate immune cell responses and trafficking during acute inflammation uncovered a novel genus of mediators produced from essential fatty acids (Figure 1). These autacoids were characterized as specialized pro-resolving mediators (SPM) given their potent biological activities in curtailing inflammation [5,6,7]. SPM are produced via the stereoselective conversion of essential fatty acids by specific biosynthetic enzymes [8,9]. Production of these autacoids is initiated by lipoxygenases (encoded by the ALOX genes), primarily ALOX5, ALOX12, ALOX15 and cyclooxygenase (COX)-2 [6,7,8,9,10,11,12,13,14,15,16]. Among the first described SPM were the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)-derived resolvins, termed as E-series (RvE) and D-series resolvins (RvD), respectively [6,7]. More recently we found that, in addition to arachidonic acid (AA), EPA and DHA, n-3 docosapentaenoic acid (n-3 DPA) is also substrate in the formation of novel SPM that share biosynthetic pathways with the DHA-derived resolvins, protectins and maresins [11,12]. Table 1 provides a summary of the mediators that form part of the distinct SPM families together with their complete stereochemistries where established.
An extensive amount of work has detailed the biology of these molecules in governing inflammation, demonstrating that each of these autacoids is able to regulate the production of pro-inflammatory mediators (including cytokines and eicosanoids), leukocyte trafficking to the site of inflammation, the ability of phagocytes to uptake bacteria and the ability of macrophages to clear apoptotic cells [8,17,18,19,20,21,22,23,24,25]. Mounting evidence demonstrates SPM are central in regulating adaptive immunity, including T-cell effector function [26,27,28], regulatory T-cell differentiation [24,28] and B-cell antibody class switch [29,30]. Furthermore, the biology of these molecules extends beyond the regulation of immune cell responses, with several studies detailing the ability of these autacoids in promoting epithelial cell migration [31,32], the production of bactericidal proteins by epithelial cells [33,34] and the regulation of endothelial cell responses [35,36] among others. Notably, their biological activities are mediated by cognate receptors that form part of the G-protein-coupled receptor family [8,37]. Deletion of these receptors or loss of SPM production have been linked with the exacerbation of inflammation in several experimental disease settings [38,39]. Together, this wealth of knowledge underscores a central role of SPM in governing both immune and stromal cell responses to limit inflammation.
Further supporting a role for SPM in the onset and/or progression of inflammatory disorders are studies conducted by several laboratories in a wide range of human disease settings, including rheumatoid arthritis [40,41], periodontal disease [42] and atherosclerosis [43,44], demonstrating that SPM production and/or activity is dysregulated. Intriguingly, several studies have also detailed a role for these molecules in mediating the biological activities of a number of commonly used drugs, such as aspirin [45,46] and statins [11,47,48].
As with many small molecules, the state-of-the-art methodologies employed in the identification and quantitation of SPM are those based on liquid chromatography tandem mass spectrometry. This approach provides the distinct advantage of being able to simultaneously measure the concentrations of multiple molecules originating from different types of biological tissues with a relatively high degree of sensitivity and specificity. Liquid chromatography is an essential step in the analytical process, given that many of the SPM are either positional or chiral isomers of each other. Using reverse phase chromatography one can separate many of these species (Figure 2). Intriguingly, reverse phase chromatography using C18 columns is not only able to separate positional isomers but also chiral isomers of the SPM. This is in line with what is observed for other lipid mediators, such as leukotriene B4, whereby, using reverse phase chromatography, one can easily separate both the double bond and positional isomers of this molecule [49]. Mediator separation using reverse phase chromatography can be achieved using both high pressure liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UPLC) systems.
Identification and quantitation of SPM is achieved using tandem mass spectrometers. There is a range of instrumentation employed for the quantitation of lipid mediators that take advantage of different technologies, including different ionization and detector technologies. The analysis of SPM is generally dependent on electro spray ionization and the use of multiple reaction monitoring (MRM) methodologies. This relies on the use of pre-developed methodologies with pre-set transitions for each of the mediators of interest, where the precursor ion and a characteristic product ion are monitored for each molecule. In the elaboration of such methodologies, great care must be taken to ensure that the instrument parameters being used are carefully tuned for each molecule and for each of the transitions, given that in many instances even minor changes in parameters, such as collision energies, may result in marked changes in sensitivity. The advantage of using a targeted approach with pre-defined MRM transitions is that instrument sensitivity can be increased by using a schedule for when each of the transitions should be monitored, thereby reducing the time that the instrument spends on the acquisition of data in regions of the chromatogram which are not of interest. Given the strong reliance on MRM methodologies, and the fact that many of the molecules being monitored are positional isomers, triple quadrupole mass spectrometers are extensively used in the study of SPM. Given the expanding utility and improvements in available instrumentation and methodologies, in the present review we will discuss the evidence supporting the utility of distinct SPM as diagnostic and/or predictive biomarkers in a range of inflammatory conditions.

2. Additional Quantitation Methodologies

While targeted LC-MS/MS remains the gold standard approach for the identification and quantitation of SPM, other approaches have been used for the quantitation of lipid mediators. These include ultraviolet (UV)-HPLC and ELISAs. UV-HPLC based methodologies have a key limitation which is related to the sensitivity of the UV, thereby limiting its use to those tissues and biological systems where SPM concentrations are high. Furthermore, drug metabolites that display similar chromatographic behavior may also interfere with the detection of these molecules, thereby further limiting the application of this approach. ELISAs have been used for the measurement of lipid mediators, including leukotrienes and prostaglandins, in biological systems for many decades. In recent years, ELISAs have been developed for the measurement of SPM which are widely employed in the measurement of these molecules in human tissues and experimental systems. These types of assays allow many samples to be analyzed simultaneously at relatively low cost, thereby expanding its potential utility in diagnostics. One aspect that has been viewed unfavorably by some investigators is the specificity of this methodology to identify the target mediator without interference from matrix or other isomers. Another aspect to be mindful of is that ELISAs should be used with caution, and investigators should ensure that ELISAs being used for the quantitation of SPM have been appropriately validated to minimize the likelihood of erroneous identification/quantitation of these autacoids in biological systems. It should be noted that studies comparing results obtained from ELISAs with those obtained using mass spectrometry suggest that whilst the reported absolute amounts usually differ, the relative quantities and, most importantly, the direction of change (i.e., upregulation or downregulation) observed between different study groups remain similar. This aspect is encouraging since it suggests that with the appropriate benchmarking and method development, ELISAs might prove to be useful in the development of scalable diagnostics. This aspect is further enhanced by recent technological developments including the development of aptamer technologies [50] and small molecule arrays [51].

3. Sample Preparation and Quantitation Methodologies

Given the relatively low abundance of these molecules, it is essential that samples are extracted from their biological matrix using solid phase extraction methodologies. The main methodologies employed for this process involve reverse phase C18 columns. Normal phase (silica) and ion exchange (anion or cation) phase have also been applied for the extraction of SPM and other lipid mediators with different phases, providing select advantages in both limiting matrix carryover and the extraction of specific lipid mediator species based on their hydrophobicity and ion-exchange capacity [52]. Identifying suitable extraction methodologies that minimize matrix carryover and ensure maximal recovery of these molecules is a critical step given that matrix interference will markedly influence the sensitivity of the assay and potentially result in false negative results.
In order to facilitate the identification and quantitation of SPM, isotope labelled internal standards are added to the samples prior to extraction. The most widely used isotope labelled standards in the study of lipid mediators are those labelled using deuterium labels. The use of this isotope results in molecules that display essentially similar chromatographic and fragmentation profiles but with a distinct molecular mass, meaning that they can be easily distinguished from the biological forms. These molecules are used to monitor inter-run changes in the expected retention times of the biological molecules that may result from matrix-associated changes in the ability of the molecules to interact with the stationary phase or technical issues. Furthermore, because these standards are added prior to sample extraction, their absolute quantities in each of the samples can be used to estimate sample recoveries, thereby facilitating the quantitation of the biological molecules in each of the samples. To achieve this, isotope labels representing distinct regions of the chromatogram and distinct carbon lengths and number functional groups (e.g., hydroxyl groups or peptide moieties) are employed as surrogates to calculate recoveries [40,53]. The limitation of this approach is that, to date, only a limited number of internal standards are available and therefore one cannot precisely calculate the absolute concentrations of those molecules where the corresponding isotope labelled standard is not available. Therefore, caution must be taken in the interpretation of such quantitative data, especially for those molecules where the corresponding isotope labelled molecules is not available since the absolute amounts may not necessarily correspond to the calculated amount using this surrogate approach.

4. Diagnostic Utility of SPM in Infections

Studies investigating the biology of SPM in the context of bacterial infections have elucidated a role for these mediators in regulating host immunity. For example, in several experimental systems, resolvin (Rv) D1 and its 17R-epimer exert protective activities against several bacterial pathogens, including Citrobacter rodentium [54], Escherichia coli [55,56] and Staphylococcus aureus [55], via the regulating host immune response to downregulate the amplitude of the inflammatory process while enhancing the ability of phagocytes to clear the invading pathogens. These biological actions were also observed in models of polymicrobial sepsis where SPM, including RvD2 and Maresin (MaR) 1 [57], limit phagocyte recruitment to the site of infection, reduce the production of local and systemic inflammatory mediators, promote bacterial clearance and enhance survival. These biological activities of SPM were also recently found to be retained in humans. Using an elegant model of dermal bacterial infection, Motwani et al. demonstrated that local administration of SPM, namely LXB4, RvE1, RvD2 and 17R-RvD1, at concentrations equivalent to those measured in the tissues during active infection accelerated the resolution of inflammation [58].
Given these remarkable host protective activities of SPM in facilitating the clearance of bacteria, the question of whether the endogenous levels of these molecules might shed light on disease status in humans has arisen. In this context Norris et al. found that SPM production was downregulated in healthy volunteers following LPS challenge [59]. Intriguingly, this dysregulation of SPM biosynthesis was observed even in volunteers that were previously administered omega-3 supplements and recovers with time after challenge. These observations suggest that exposure of the host to microbial components might disrupt SPM production, facilitating disease. Studies with septic patients support the observation that SPM production is dysregulated during infections [60]. Indeed, here it was observed that plasma SPM concentrations, together with concentrations of classic eicosanoids, were linked with 28-day mortality. Interestingly, this distinction between patients that survived and those that did not was observed to arise as early as 24 h after admittance to the Intensive Care Unit. This observation that SPM concentrations might be linked with patient outcome was also made in patients with meningeal tuberculosis [61]. As a result, overall levels of these mediators in the cerebrospinal fluid of patients with meningeal tuberculosis were markedly reduced in those patients that subsequently succumbed to the disease when compared with those that survived.
The biological actions of SPM are also reported to extend into the regulation of host immunity during viral infections. For example, the DHA-derived RvD1 and its precursor 17-hydroxy-docosahexaenoic acid (17-HDHA) regulate B-cell response during H1N1 infections in mice by promoting antibody class switch [62]. The Protectin (PD) family of mediators regulate viral propagation by inhibiting intracellular viral RNA transport mechanisms [63]. Similarly, the EPA-derived RvE1 reduces effector T-cell and neutrophil-mediated propagation of inflammation during herpes simplex virus infections [64]. Recent studies also suggest a role of these mediators in regulating host immune responses to severe acute respiratory syndrome corona virus (SARS-CoV)-2. In these, RvD1 and RvD2 govern the production of inflammatory cytokines by macrophages, including IL-8 and TNF-a, via the regulation of micro-RNA expression [65]. Studies in humans demonstrate that SPM levels in either broncho alveolar lavage fluids or serum are linked with disease outcome in viral infections. Archambault et al. found that D-series resolvins and LXA4 concentrations, together with levels of several pro-inflammatory eicosanoids were increased in bronchoalveolar lavages of intubated COVID-19 patients [66]. Similar observations were also made in serum from patients with COVID-19, where levels of these autacoids were elevated with increased disease severity [67]. Thus, these findings support a potential role for measuring SPM concentrations in patients with both bacterial and viral infections to aid in the management of these patients by establishing early in the course of their hospitalization their disease trajectory and therefore design optimal treatment strategies.

5. Pro-Resolving Mediators as Biomarkers in Chronic Inflammatory Conditions

Amongst the key biological activities of SPM is their ability to limit leukocyte infiltration to the site and counter-regulate the formation of pro-inflammatory mediators [68]. Thus, there is extensive interest in establishing whether the production of these molecules is disrupted in chronic inflammatory conditions and whether the pharmacological administration of SPM may represent a useful approach for treating such conditions. Indeed, studies conducted by many groups demonstrate that SPM production becomes dysregulated in chronic inflammatory settings and that the use of these mediators or their analogues may limit disease progression and in some instanced even reverse some of the damage. Table 2 provides a non-exhaustive summary of studies reporting on SPM levels in human disease and their regulation by supplementation strategies. Interested readers are also referred to a recent comprehensive review detailing the biological actions of SPM in a range of disease setting [8]. In the remainder of this section, we will discuss some of the evidence related to a select group of these conditions. We also direct interested readers to other, more comprehensive reviews that detail aspects of SPM biology that are not covered below [8,21,69,70,71,72,73]:

5.1. Periodontal Disease

Periodontal disease is characterized by unremitting inflammation that results in significant damage to the periodontium. Studies in experiential settings demonstrate that SPM exert potent activities in the regulation of periodontal inflammation by limiting leukocyte trafficking to the site and downregulating the production of pro-inflammatory mediators. These molecules also promote bone and tissue repair in several models of periodontal disease [36,42,44]. In recent studies found that levels of LXA4, PD1 and MaR1 in salivary tissues of patients with periodontal inflammation were correlated with disease severity. Intriguingly, while LXA4 levels were observed to be negatively correlated with disease severity, those of PD1 and MaR1 were positively correlated. These results suggest that SPM biosynthetic pathways, or potentially their degradation pathways, are differentially regulated in disease settings likely as an attempt of the host immune response to counter the ongoing inflammatory processes [82].

5.2. Rheumatoid Arthritis

This condition, as with periodontal disease, is characterized by unremitting inflammation that leads to progressive tissue destruction. Both the innate and adaptive arms of the immune response are implicated in the pathogenesis of RA. Studies in pre-clinical models demonstrate that several SPM, including RvD1 [95], RvD3 [96] and MaR1 [97], regulate disease progression. In addition, MaR1 was recently found to limit nociceptive hypersensitivity linked with inflammatory arthritis [98]. In humans, Barden et al. observed that synovial RvE2 concentrations were negatively associated with pain score whereas overall plasma SPM concentrations were negatively correlated with erythrocyte sedimentation rate, a marker of disease activity [41]. Separate studies report that serum concentrations of LXA4, RvD1 and RvE1 are significantly reduced in patients with active RA when compared with those in remission [99]. Furthermore, plasma SPM concentrations were observed to reflect synovial disease phatotype in RA patients [40]. This observation is of interest since synovial disease phototypes were recently observed to be linked with a differential response to disease-modifying anti-rheumatic drugs [40]. Furthermore, current approaches to stratifying patients using their pathotype are somewhat cumbersome since they involve ultrasound guided biopsies. Therefore, blood SPM concentrations might provide a useful alternative for patient stratification.

5.3. Vascular Disease

Atherosclerosis is characterized by the systemic activation of immune cells that are then recruited into the vascular wall and contribute to the accumulation of lipids. Studies in experimental systems demonstrate that loss of ALOX15, a key enzyme in SPM production, was linked with increased vascular lipid load in atherosclerosis prone mice [100]. Pharmacological studies demonstrate that SPM regulate various aspects of disease development. Whereby, 15-epi-LXA4 and RvE1 were observed to limit the migration of human saphenous vein SMCs and decrease phosphorylation of the platelet-derived growth factor receptor-β [101]. RvD1 was also observed to exert protective activities in regulating atheroprogression by improving the uptake of apoptotic cells within atherosclerotic lesions, reducing lesional oxidative stress and increasing the thickness of the fibrous cap. RvD2 and MaR1 regulate disease progression in experimental settings this time by reducing macrophage accumulation in vascular tissues and increasing the number of smooth muscle cells [102]. By contrast, RvD5n-3 DPA decreased disease progression by limiting platelet leukocyte interactions [103].
SPM levels are also observed to be altered in humans where the concentrations of RvD1 are markedly reduced in vulnerable regions from human carotid atherosclerotic plaques [81]. Plasma levels of 15-epi-LXA4 are significantly lower in patients with symptomatic peripheral artery disease than in healthy volunteers [101]. Similarly, peripheral blood n-3 DPA-derived resolvin (RvDn-3 DPA) concentrations are lower in patients with cardiovascular disease when compared with those observed in healthy volunteers [103]. This reduction in RvDn-3 DPA concentrations is linked with an increased activation of circulating phagocytes [103], suggesting that these molecules might be useful indicators of vascular disease.

5.4. Allergic Inflammation

The biological activities of SPM are also described in allergic conditions including allergic airway diseases such as asthma and allergic rhinitis. In recent studies, RvE3 was observed to reduce the total numbers of inflammatory cells and eosinophils recruited into the lung of mice sensitized and challenged with house dust mite. This mediator also reduced the levels of IL-23 and IL-17 in lavage fluid and suppressed the expression of IL-23 and IL-17A mRNA expression in lung and peribronchial lymph node. Importantly, these cellular and molecular changes were linked with a reduction in lung resistance in mice treated with RvE3 [104]. In a murine model of allergic asthma, RvE1 promoted a decrease in leukocyte recruitment into the lung and downregulated the expression of pro-inflammatory cytokines in lavage fluids and in macrophages [105]. In separate studies, the DHA-derived RvD1 and AT-RvD1 were observed to exert protective activities in murine models of asthma. Here, RvD1 markedly decreased airway eosinophilia and mucus metaplasia and downregulated the expression of IL-5 and IκBα degradation. AT-RvD1 also regulated lung inflammation in these settings, leading to a marked decrease in the resolution interval for lung eosinophilia and the expression of inflammatory peptide and lipid mediators, actions that together contributed to an accelerated resolution of airway hyperreactivity to methacholine [106].
The protective activities of SPM are also observed with phagocytes from patients with asthma whereby AT-RvD1 reduced TNF-α levels in peripheral blood mononuclear cells from patients with severe asthma stimulated with lipopolysaccharide or Dermatophagoides pteronyssinus. This SPM also increased phagocytosis of apoptotic neutrophils by monocytes from patients with severe asthma [107]. PD1 was recently observed to display protective activities in regulating eosinophil biology, a key cell type linked with the propagation of allergic inflammation. Indeed, this SPM limited chemotaxis of these cells towards CCL11/eotaxin-1 and 5-oxo-eicosatetraenoic acid. PD1 also modulated the expression of the adhesion molecules CD11b and L-selectin on these cells [108].

5.5. COVID-19

The potent bioactions of SPM in regulating both innate and adaptive immune responses, together with a mounting body of evidence suggesting that the production of these mediators may be dysregulated in hospitalized patients with COVID-19, have sparked interest in evaluating whether these molecules could provide novel leads in treating the excessive inflammatory response observed in these patients. While the direct evidence on the therapeutic potential of these molecules in COVID-19 is at present limited, studied performed thus far in these patients, as well as in other respiratory viral infections such as H1N1, are encouraging. Indeed, in recent studies we found that the incubation of phagocytes from COVID-19 with SPM rectifies their abilities to uptake and kill bacteria. These mediators also downregulate the expression of activation markers on circulating phagocytes, including tissue factor (CD142), which was recently linked with an increased risk of thrombosis in these patients [109]. Furthermore, RvD2, RvD3, MCTR3 and PCTR3 downregulated the expression of inflammatory cytokines in monocyte-derived macrophages from patients with COVID-19, including IFNγ and TNF-α [53]. In murine studies with influenza infections, the protectin family of mediators was observed to both regulate the inflammatory response and limit viral replication by inhibiting the viral export machinery [63]. By contrast, the D-series resolving precursor 17-HDHA increased antigen-specific Ab titers to pH1N1 virus. 17-HDHA also increased the number of antigen-specific antigen-secreting cells present in the bone marrow. Intriguingly, the 17-HDHA-mediated-increased antigen production was more protective against live pH1N1 influenza infection in mice [62]. Together, these findings suggest that SPM may hold promise as new therapeutics for COVID-19 by reprogramming both the innate and adaptive arms of the immune response to decrease inflammation and enhance anti-viral immunity.

6. SPM as Biomarkers for Determining Therapeutic Efficacy of Anti-Inflammatory Drugs

Studies investigating the mechanisms of action of several widely used therapeutics such as statins and aspirin suggest a role for SPM in mediating their anti-inflammatory activities (Figure 3). For example, aspirin, in addition to inhibiting prostaglandin and thromboxane formation, promotes the formation epimeric forms of the resolvins, protectins and lipoxins via the acetylation of COX-2 [7,45]. Indeed, this reaction ablates the ability of the enzyme to catalyze the formation of PGG2 while retaining its ability to oxygenate AA on carbon 15 and DHA on carbon 17. These mediators display similar binding affinities to the cognate receptor for the ALOX-derived molecules while displaying enhanced stability to metabolic inactivation [110]. The ability of aspirin, in particular low dose aspirin, to upregulate the formation of these molecules was also established in humans, whereby Chiang and colleagues demonstrated in a randomized trial that low dose aspirin, at variance to high dose aspirin, upregulated plasma 15-epi-LXA4 (also referred to as AT-LXA4) concentrations [111]. Intriguingly, the ability of aspirin to increase 15-epi-LXA4 concentrations in healthy volunteers was observed to be dependent on gender and age. Where, a positive correlation was observed in females with age, whilst a negative correlation was found in males in the ability of aspirin to increase plasma 15-epi-LXA4 [112]. The gender-specific differences in AT-SPM regulation appear to be tissue or potentially condition-specific. In a separate study, the regulation of 15-epi-LXA4 by aspirin in gingival crevicular fluid of patients with periodontal disease was not observed to be different between males and females [113]. These findings suggest that while aspirin may regulate the formation of AT-SPM in distinct tissues; factors such as age, gender and disease may have an influence on the individual mediators being regulated (i.e., 15-epi-LXA4 vs. 17R-RvD1 vs. 17R-PD1) and the direction of change (i.e., upregulated or downregulated). Thus, future studies will need to establish which of these autacoids is diagnostic of aspirin efficacy in specific patient populations and target tissue or fluid (e.g., plasma).
Another class of drugs that regulates SPM formation is the statins. In experimental lung inflammation, Lovastatin increases 15-epi-LXA4 concentrations via the upregulation of 14,15-epoxyeicosatrienoic acid by airway epithelial cells [47]. Atorvastatin was observed to also upregulate 15-epi-LXA4 levels in the heart, albeit via a different mechanism to that observed in the lung. Indeed, in this context atorvastatin regulates cyclooxygenase-2 and 5-lipoxygenase activity to increase 15-epi-LXA4 levels [48]. We recently observed that, in vascular endothelial cells, atorvastatin also regulates COX-2 activity by promoting nitrosylation of the enzyme, a reaction that was dependent on the activity of the nitric oxide synthase [11]. This mechanism contributed to the upregulation of 13-series resolvins (RvT) that display potent immune regulatory activity on phagocytes. Indeed, pharmacological inhibition of COX-2 activity blocked RvT formation and reversed the protective activities of atorvastatin in both experimental joint inflammation and bacterial infections [11,114].
Recent studies suggest that dexamethasone may also upregulate SPM formation [115]. In experimental allergic inflammation levels of the DHA-derived protectins and those of resolvin pathway marker 17-HDHA were increased by this corticosteroid. We also observed that this regulation of SPM levels by this potent anti-inflammatory drug was retained in human airway inflammation, with levels of several SPM, including the DHA-derived PD1 and the n-3 DPA-derived MaR1n-3 DPA, being markedly upregulated in plasma of patients with COVID-19 treated with dexamethasone [53]. Intriguingly, these observations were linked with the upregulation of several SPM biosynthetic enzymes in circulating leukocytes from patients treated with dexamethasone [53]. These observations suggest that dexamethasone increases SPM formation by regulating the expression of their biosynthetic enzymes.

7. Potential for the Use of SPM as Biomarkers for Determining the Utility of Omega-3 Supplements in Regulating Inflammation

Omega-3 supplements have long been held to exert beneficial actions in the regulation of inflammation with a plethora of studies in experimental systems supporting their utility in a range of inflammatory conditions, including cardiovascular disease and arthritis [70,116,117]. The evidence in humans has been less clear-cut, with studies observing both beneficial and potentially harmful effects or no effects. One should note that studies in humans have used a wide range of doses as well as forms of these fatty acids and the influence of these variables on their bioavailability, amongst others, are not fully understood. Given that omega-3 fatty acids are substrates in the formation of SPM, several groups have investigated whether the beneficial actions of these supplements are at least in part linked with the upregulation of these protective autacoids. Studies conducted in humans appear to support this hypothesis. In patients with minor cognitive impairment, the administration of an omega-3 supplement increased plasma RvD1 concentrations and improved cognitive function in these patients [118]. Of note, the epimeric form of RvD1, 17R-RvD1, which exerts its biological actions via the same receptors, was previously reported to also limit post-operative cognitive decline in an experimental model of surgical-induced cognitive decline [119]. In patients with chronic kidney disease, supplementation with omega-3 fatty acids led to an increased production of SPM, primarily RvE1, RvE2, RvE3 and RvD5, by isolated peripheral blood neutrophils. This increase in SPM production was linked with a decrease in myeloperoxidase levels, a marker of neutrophil activation, in plasma from patients receiving omega-3 fatty acids [120].
Recent studies have also started to address the pharmacokinetics of omega-3 supplements by using SPM as functional biomarkers. In studies performed in healthy volunteers, plasma SPM concentrations reached a maximum as early as 2 h after the oral ingestion of the supplement [121]. This increase was also dependent on the initial dose of omega-3 supplement provided, establishing a link between substrate availability and conversion to SPM. Furthermore, in these studies plasma levels of several SPM were correlated with changes in both peripheral blood phagocyte and platelet activation. Of note, plasma levels of these autacoids also rapidly decreased in peripheral blood of healthy volunteers given a single dose of omega-3 supplement, returning to baseline values between 2–24 h after supplementation depending on the supplement dose administered. This observation is in line with the biology of SPM, whereby, as autacoids, these molecules are further metabolized and cleared from the tissue. Experimental evidence obtained so far on the kinetics of these processes has primarily focused on in vitro systems, demonstrating that the rates of conversion of these autacoids varies between different molecules [110,122,123]. Furthermore, some of the further metabolites described for these molecules retain the biological activities of the parent molecule [110,122,123]. These aspects will need to be taken into consideration when devising strategies to monitor supplement efficacy in patient populations.
Notably, in instances where supplements are administered over longer periods to those employed in our recent studies, there may be some flexibility regarding the timing employed between supplementation and sample collection. In this context, we recently evaluated plasma SPM concentrations in patients with peripheral artery disease. These patients were administered the supplement for 7 days and then blood was collected. Here, plasma SPM levels of these autacoids were elevated several hours after the administration of the last supplement dose compared to pre-supplement levels. These findings suggest that chronic administration may result in sustained substrate release, likely from esterified pools that can support SPM formation for longer periods to those achieved with a single equivalent supplement dose. Furthermore, plasma SPM concentrations in patients with periphery artery disease were increased in dose-dependent manner, an increase that was linked with changes in peripheral blood phagocytes response [124]. Together these observations lend support to the potential utility of peripheral blood SPM as functional biomarkers in determining the efficacy of supplementation at regulating inflammation.

8. Utility of Machine Learning in Biomarker Identification

Available evidence from both experimental systems and animal models suggest that distinct mediators are regulated in a context, tissue and condition-specific manner. Therefore, methodologies for identifying and corroborating the potential utility of individual SPM as potential biomarkers in each disease setting are essential in facilitating their clinical application.
Identifying biomarkers by a conventional approach, meaning identifying a candidate and running tests to validate it, requires a great amount of biological evidence and is time-consuming, expensive and risky. To date, new techniques to generate and collect large amounts of data, such as mass spectrometry, have been used in biological and medical research, including drug discovery and biomarker development, for the analysis of a high number of molecules improving the chances of identifying biomarkers of interest [125].
The value of data generated by mass spectrometry depends on the analysis strategies employed. Machine learning methodologies, algorithms to build mathematical models based on patterns and interferences that then can make decisions [126] have been applied to metabolomics data to diagnose illness and, in a more advanced application, to classify and predict outcomes of different diseases and therefore contribute to the identification of biomarkers associated with them. These biomarkers can be used to determine the efficacy of pre-existing treatments and diagnostic strategies [126,127,128].
Biological and medical data are complex, containing multiple layers of information that are usually incomplete, heterogeneous, noisy and present unwanted variability (e.g., operator and batch effects) [128]. Different from univariate association analyses, machine learning can address some of the issues associated with biological data while also providing strategies for considering unknown associations between the different variables present in the samples [129].
Machine learning strategies work as a flow of knowledge: the model is built by learning from sample data, known as training data, and then applies the knowledge to make predictions or classify data [126]. Three steps must be followed for good practice in machine learning application: training, in which the model uses the training dataset to create the classification model, validation, in which the performance of the model is assessed by reshuffling of the training data, and evaluation, which consists of evaluating the model using a new dataset (Figure 4).
Different supervised machine learning methodologies have been used to identify biomarkers through the years, all of them with their pros and cons. Among the most popular are Bayesian classifiers [130], artificial neural networks [131], support vector machine [132] and random forest [133]. The most common disadvantages of these strategies are that they require a great number of samples per group, overfitting (the model is only good to classify the training data), mishandling of multicollinearity (models not identifying correlations between different variables) and sensitivity to the presence of outliers.
When applied correctly, machine learning helps to understand complex biological data without missing unknown interactions between the features and leading to the identification of relevant biomarkers in different conditions. For this to occur, the training data needs to be strong enough (a large number of samples and features) to create reliable models. A pre-processing step derived from exploratory analysis can be helpful to make the data stronger by identifying underlying correlations, taking out outlier values and scaling the features. Choosing the best machine learning methodology is always fundamental and it will depend on the available data and the biological question that needs to be answered. It is important to consider the disadvantages of each methodology so that measures can be taken. Finally, evaluating the models in new and independent datasets is the most important step if the purpose of the machine learning strategy is to create a model able to predict between classes, identify biomarkers and give an insight into the biological processes behind the prediction logic.
We recently applied these models to evaluate whether they can be utilized to identify biomarkers that are prognostic of treatment responsiveness. Using machine learning, we devised models that were trained using plasma lipid mediator concentrations of patients that responded to disease modifying anti-rheumatic drugs, front line therapeutics in rheumatoid arthritis and plasma from patients that did not respond [40]. These models identified candidate mediators that were then validated using plasma from a second cohort of patients, demonstrating that these biomarkers were prognostic of treatment responsiveness with an accuracy in excess of 85% [40]. Due to the relatively small number size, this initial study used data obtained from both males and females for both model building and validation. Future studies will need to evaluate whether the sensitivity and accuracy of these models can be further enhanced by evaluating lipid mediator concentrations from different sexes separately in light of findings suggesting that sex and sex hormones influence lipid mediator production [134,135]. Nonetheless, given that patient responsiveness to a whole host of therapeutics is acknowledged to vary and may expose patients to unwanted side effects of given therapeutics, prognostic biomarkers may be a game changer in the development of a personalized medicines approach that is better suited to the individual patient.

9. Current Limitations and Future Directions

Evidence presented in previous sections supports the potential utility of specific SPM as biomarkers in distinct disease settings. It should be emphasized that given the dynamic regulation of these autacoids and the evidence available thus far, it is likely that there will not be one or a small number of SPM that may be universally useful as biomarkers in a wide range of disease settings. Indeed, it is likely that one or a panel of SPM may be only useful in select disease settings, and likely only for a given tissue or biological sample. This aspect provides both advantages, in that one can have specific diagnostic tests for given conditions, as well as disadvantages, in that tests will need to be developed and validated for every application, thereby increasing the costs. One also has to recognize that available evidence will still need to be validated in prognostic studies, ideally in a multi-center setup. This will help to establish both the robustness as well as the generalizability of potential biomarkers, especially in light of recent studies that suggest a differential regulation of lipid mediators in different ethnic groups [78]. Another aspect that will require careful consideration is platforms to be used for the analysis of these molecules. It is widely appreciated that there are marked differences in the ability of different mass spectrometry platforms to identify small molecules, and in particular low abundance molecules such as lipid mediators. Therefore, bench marking of instrumentation and methodologies using standard reference materials will be essential in order to ensure that these are sufficiently sensitive to quantify these molecules in a robust manner. Finally, current methodologies for sample preparation and mediator identification are somewhat laborious, limiting the sample throughput. Nonetheless, such limitations should not preclude further development in this field given that a number of steps to address these limitations have already been made with the availability of commercial standard reference materials, the constant improvements in instrumentation and the evolution of artificial intelligence. Furthermore, the extensive body of literature outlining the important biological role that these molecules play in regulating host immunity provides further impetus for identifying innovative approaches to overcoming these limitations. Deployment of SPM-based diagnostics is also likely to be complementary to those currently used in clinical practice, such as, for example, high sensitivity C-reactive protein (CRP) or erythrocyte sedimentation rates (ESR). This is because the latter biomarkers are a readout of ongoing inflammation and are therefore endpoint molecular measurements to determine whether a specific treatment has had its desired effects. By contrast, since, as discussed in the previous sections, SPM carry potent immune-regulatory activities, changes in their concentrations in biological tissues are likely to be linked with changes in immune cell behavior. Whereby, for example, increases in AT-SPM concentrations in patients treated with aspirin or an upregulation in RvT levels in patients treated with atorvastatin will lead to changes in phagocyte behavior and a downregulation of inflammatory mediator production [7,11]. Similarly, an upregulation of MCTRs in patients with bacterial infections may be linked with a potential beneficial effect of the treatment being given to these patients given that these autacoids upregulate the ability of phagocytes to clear bacteria. Furthermore, MCTRs promote tissue repair and regeneration, thereby increasing the resilience of the host to the infectious insult [10]. Consequently, diagnostics based on these mediators may be useful as either predictors or early measures of whether a given treatment is likely to limit inflammation and decrease endpoint measures such as CRP and ESR. Therefore, while acknowledging that there is still some way to go before the potential for SPM-based biomarkers can be harnessed, we look to the future with confidence that SPM may indeed be useful as novel biomarkers that will facilitate the development of personalized medicines.

Author Contributions

J.D. wrote the initial draft and J.D., E.A.G. and C.C.J. contributed to manuscript preparation. All authors have read and agreed to the published version of the manuscript.

Funding

Work in J.D. laboratory is supported by funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant no: 677542) and the Barts Charity (grant no: MGU0343) to J.D. J.D. is also supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (grant 107613/Z/15/Z).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

We thank Romain A Colas for assistance in preparation of this review.

Conflicts of Interest

J.D. is scientific founders and director of Resolomics Limited. J.D. is also an inventor on patents related to the composition of matter and/or use of pro-resolving mediators as both diagnostics and therapeutics that are assigned to Queen Mary University of London and the Brigham and Women’s Hospital. E.A.G. is an inventor on a patent related to the use of pro-resolving mediators as diagnostics assigned to Queen Mary University of London. C.C.J. declares no competing interests.

References

  1. Collins, F.S.; Varmus, H. A new initiative on precision medicine. N. Engl. J. Med. 2015, 372, 793–795. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Jameson, J.L.; Longo, D.L. Precision medicine—Personalized, problematic, and promising. N. Engl. J. Med. 2015, 372, 2229–2234. [Google Scholar] [CrossRef] [Green Version]
  3. Aletaha, D.; Smolen, J.S. Diagnosis and Management of Rheumatoid Arthritis: A Review. JAMA 2018, 320, 1360–1372. [Google Scholar] [CrossRef] [PubMed]
  4. Ibrahim, A.; Ahmed, M.; Conway, R.; Carey, J.J. Risk of Infection with Methotrexate Therapy in Inflammatory Diseases: A Systematic Review and Meta-Analysis. J. Clin. Med. 2018, 8, 15. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Levy, B.D.; Clish, C.B.; Schmidt, B.; Gronert, K.; Serhan, C.N. Lipid mediator class switching during acute inflammation: Signals in resolution. Nat. Immunol. 2001, 2, 612–619. [Google Scholar] [CrossRef] [PubMed]
  6. Serhan, C.N.; Clish, C.B.; Brannon, J.; Colgan, S.P.; Chiang, N.; Gronert, K. Novel functional sets of lipid-derived mediators with antiinflammatory actions generated from omega-3 fatty acids via cyclooxygenase 2-nonsteroidal antiinflammatory drugs and transcellular processing. J. Exp. Med. 2000, 192, 1197–1204. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  7. Serhan, C.N.; Hong, S.; Gronert, K.; Colgan, S.P.; Devchand, P.R.; Mirick, G.; Moussignac, R.L. Resolvins: A family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals. J. Exp. Med. 2002, 196, 1025–1037. [Google Scholar] [CrossRef] [Green Version]
  8. Chiang, N.; Serhan, C.N. Specialized pro-resolving mediator network: An update on production and actions. Essays Biochem. 2020, 64, 443–462. [Google Scholar] [CrossRef]
  9. Serhan, C.N.; Dalli, J.; Colas, R.A.; Winkler, J.W.; Chiang, N. Protectins and maresins: New pro-resolving families of mediators in acute inflammation and resolution bioactive metabolome. Biochim. Biophys. Acta 2015, 1851, 397–413. [Google Scholar] [CrossRef] [Green Version]
  10. Dalli, J.; Chiang, N.; Serhan, C.N. Identification of 14-series sulfido-conjugated mediators that promote resolution of infection and organ protection. Proc. Natl. Acad. Sci. USA 2014, 111, E4753–E4761. [Google Scholar] [CrossRef] [Green Version]
  11. Dalli, J.; Chiang, N.; Serhan, C.N. Elucidation of novel 13-series resolvins that increase with atorvastatin and clear infections. Nat. Med. 2015, 21, 1071–1075. [Google Scholar] [CrossRef] [PubMed]
  12. Dalli, J.; Colas, R.A.; Serhan, C.N. Novel n-3 immunoresolvents: Structures and actions. Sci. Rep. 2013, 3, 1940. [Google Scholar] [CrossRef] [PubMed]
  13. Dalli, J.; Ramon, S.; Norris, P.C.; Colas, R.A.; Serhan, C.N. Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways. FASEB J. 2015, 29, 2120–2136. [Google Scholar] [CrossRef] [Green Version]
  14. Freedman, C.; Tran, A.; Tourdot, B.E.; Kalyanaraman, C.; Perry, S.; Holinstat, M.; Jacobson, M.P.; Holman, T.R. Biosynthesis of the Maresin Intermediate, 13S,14S-Epoxy-DHA, by Human 15-Lipoxygenase and 12-Lipoxygenase and Its Regulation through Negative Allosteric Modulators. Biochemistry 2020, 59, 1832–1844. [Google Scholar] [CrossRef]
  15. Perry, S.C.; Kalyanaraman, C.; Tourdot, B.E.; Conrad, W.S.; Akinkugbe, O.; Freedman, J.C.; Holinstat, M.; Jacobson, M.P.; Holman, T.R. 15-Lipoxygenase-1 biosynthesis of 7S,14S-diHDHA implicates 15-lipoxygenase-2 in biosynthesis of resolvin D5. J. Lipid Res. 2020, 61, 1087–1103. [Google Scholar] [CrossRef] [PubMed]
  16. Tsai, W.C.; Kalyanaraman, C.; Yamaguchi, A.; Holinstat, M.; Jacobson, M.P.; Holman, T.R. In Vitro Biosynthetic Pathway Investigations of Neuroprotectin D1 (NPD1) and Protectin DX (PDX) by Human 12-Lipoxygenase, 15-Lipoxygenase-1, and 15-Lipoxygenase-2. Biochemistry 2021, 60, 1741–1754. [Google Scholar] [CrossRef]
  17. Gerlach, B.D.; Marinello, M.; Heinz, J.; Rymut, N.; Sansbury, B.E.; Riley, C.O.; Sadhu, S.; Hosseini, Z.; Kojima, Y.; Tang, D.D.; et al. Resolvin D1 promotes the targeting and clearance of necroptotic cells. Cell Death Differ. 2020, 27, 525–539. [Google Scholar] [CrossRef]
  18. Rymut, N.; Heinz, J.; Sadhu, S.; Hosseini, Z.; Riley, C.O.; Marinello, M.; Maloney, J.; MacNamara, K.C.; Spite, M.; Fredman, G. Resolvin D1 promotes efferocytosis in aging by limiting senescent cell-induced MerTK cleavage. FASEB J. 2020, 34, 597–609. [Google Scholar] [CrossRef] [Green Version]
  19. Sansbury, B.E.; Li, X.; Wong, B.; Patsalos, A.; Giannakis, N.; Zhang, M.J.; Nagy, L.; Spite, M. Myeloid ALX/FPR2 regulates vascularization following tissue injury. Proc. Natl. Acad. Sci. USA 2020, 117, 14354–14364. [Google Scholar] [CrossRef]
  20. Giannakis, N.; Sansbury, B.E.; Patsalos, A.; Hays, T.T.; Riley, C.O.; Han, X.; Spite, M.; Nagy, L. Dynamic changes to lipid mediators support transitions among macrophage subtypes during muscle regeneration. Nat. Immunol. 2019, 20, 626–636. [Google Scholar] [CrossRef]
  21. De Gaetano, M.; McEvoy, C.; Andrews, D.; Cacace, A.; Hunter, J.; Brennan, E.; Godson, C. Specialized Pro-resolving Lipid Mediators: Modulation of Diabetes-Associated Cardio-, Reno-, and Retino-Vascular Complications. Front. Pharmacol. 2018, 9, 1488. [Google Scholar] [CrossRef] [PubMed]
  22. Mattoscio, D.; Isopi, E.; Lamolinara, A.; Patruno, S.; Medda, A.; De Cecco, F.; Chiocca, S.; Iezzi, M.; Romano, M.; Recchiuti, A. Resolvin D1 reduces cancer growth stimulating a protective neutrophil-dependent recruitment of anti-tumor monocytes. J. Exp. Clin. Cancer Res. 2021, 40, 129. [Google Scholar] [CrossRef] [PubMed]
  23. Gobbetti, T.; Dalli, J.; Colas, R.A.; Federici Canova, D.; Aursnes, M.; Bonnet, D.; Alric, L.; Vergnolle, N.; Deraison, C.; Hansen, T.V.; et al. Protectin D1n-3 DPA and resolvin D5n-3 DPA are effectors of intestinal protection. Proc. Natl. Acad. Sci. USA 2017, 114, 3963–3968. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Marques, R.M.; Gonzalez-Nunez, M.; Walker, M.E.; Gomez, E.A.; Colas, R.A.; Montero-Melendez, T.; Perretti, M.; Dalli, J. Loss of 15-lipoxygenase disrupts Treg differentiation altering their pro-resolving functions. Cell Death Differ. 2021, 28, 3140–3160. [Google Scholar] [CrossRef]
  25. Libreros, S.; Shay, A.E.; Nshimiyimana, R.; Fichtner, D.; Martin, M.J.; Wourms, N.; Serhan, C.N. A New E-Series Resolvin: RvE4 Stereochemistry and Function in Efferocytosis of Inflammation-Resolution. Front. Immunol. 2020, 11, 631319. [Google Scholar] [CrossRef]
  26. Wei, J.; Mattapallil, M.J.; Horai, R.; Jittayasothorn, Y.; Modi, A.P.; Sen, H.N.; Gronert, K.; Caspi, R.R. A novel role for lipoxin A4 in driving a lymph node-eye axis that controls autoimmunity to the neuroretina. Elife 2020, 9, e51102. [Google Scholar] [CrossRef]
  27. Alvarez, C.; Abdalla, H.; Sulliman, S.; Rojas, P.; Wu, Y.C.; Almarhoumi, R.; Huang, R.Y.; Galindo, M.; Vernal, R.; Kantarci, A. RvE1 Impacts the Gingival Inflammatory Infiltrate by Inhibiting the T Cell Response in Experimental Periodontitis. Front. Immunol. 2021, 12, 664756. [Google Scholar] [CrossRef]
  28. Chiurchiu, V.; Leuti, A.; Dalli, J.; Jacobsson, A.; Battistini, L.; Maccarrone, M.; Serhan, C.N. Proresolving lipid mediators resolvin D1, resolvin D2, and maresin 1 are critical in modulating T cell responses. Sci. Transl. Med. 2016, 8, 353ra111. [Google Scholar] [CrossRef] [Green Version]
  29. Bhat, T.A.; Kalathil, S.G.; Miller, A.; Thatcher, T.H.; Sime, P.J.; Thanavala, Y. Specialized Proresolving Mediators Overcome Immune Suppression Induced by Exposure to Secondhand Smoke. J. Immunol. 2020, 205, 3205–3217. [Google Scholar] [CrossRef]
  30. Ramon, S.; Gao, F.; Serhan, C.N.; Phipps, R.P. Specialized proresolving mediators enhance human B cell differentiation to antibody-secreting cells. J. Immunol. 2012, 189, 1036–1042. [Google Scholar] [CrossRef] [Green Version]
  31. Quiros, M.; Feier, D.; Birkl, D.; Agarwal, R.; Zhou, D.W.; Garcia, A.J.; Parkos, C.A.; Nusrat, A. Resolvin E1 is a pro-repair molecule that promotes intestinal epithelial wound healing. Proc. Natl. Acad. Sci. USA 2020, 117, 9477–9482. [Google Scholar] [CrossRef] [PubMed]
  32. Zhang, F.; Yang, H.; Pan, Z.; Wang, Z.; Wolosin, J.M.; Gjorstrup, P.; Reinach, P.S. Dependence of resolvin-induced increases in corneal epithelial cell migration on EGF receptor transactivation. Investig. Ophthalmol. Vis. Sci. 2010, 51, 5601–5609. [Google Scholar] [CrossRef] [PubMed]
  33. Levy, O.; Canny, G.; Serhan, C.N.; Colgan, S.P. Expression of BPI (bactericidal/permeability-increasing protein) in human mucosal epithelia. Biochem. Soc. Trans. 2003, 31, 795–800. [Google Scholar] [CrossRef] [PubMed]
  34. Canny, G.; Levy, O.; Furuta, G.T.; Narravula-Alipati, S.; Sisson, R.B.; Serhan, C.N.; Colgan, S.P. Lipid mediator-induced expression of bactericidal/ permeability-increasing protein (BPI) in human mucosal epithelia. Proc. Natl. Acad. Sci. USA 2002, 99, 3902–3907. [Google Scholar] [CrossRef] [Green Version]
  35. Chattopadhyay, R.; Raghavan, S.; Rao, G.N. Resolvin D1 via prevention of ROS-mediated SHP2 inactivation protects endothelial adherens junction integrity and barrier function. Redox Biol. 2017, 12, 438–455. [Google Scholar] [CrossRef] [PubMed]
  36. Maekawa, T.; Hosur, K.; Abe, T.; Kantarci, A.; Ziogas, A.; Wang, B.; Van Dyke, T.E.; Chavakis, T.; Hajishengallis, G. Antagonistic effects of IL-17 and D-resolvins on endothelial Del-1 expression through a GSK-3beta-C/EBPbeta pathway. Nat. Commun. 2015, 6, 8272. [Google Scholar] [CrossRef] [Green Version]
  37. Chiang, N.; Serhan, C.N. Structural elucidation and physiologic functions of specialized pro-resolving mediators and their receptors. Mol. Aspects Med. 2017, 58, 114–129. [Google Scholar] [CrossRef]
  38. Chiang, N.; Dalli, J.; Colas, R.A.; Serhan, C.N. Identification of resolvin D2 receptor mediating resolution of infections and organ protection. J. Exp. Med. 2015, 212, 1203–1217. [Google Scholar] [CrossRef] [Green Version]
  39. Tourki, B.; Kain, V.; Pullen, A.B.; Norris, P.C.; Patel, N.; Arora, P.; Leroy, X.; Serhan, C.N.; Halade, G.V. Lack of resolution sensor drives age-related cardiometabolic and cardiorenal defects and impedes inflammation-resolution in heart failure. Mol. Metab. 2020, 31, 138–149. [Google Scholar] [CrossRef]
  40. Gomez, E.A.; Colas, R.A.; Souza, P.R.; Hands, R.; Lewis, M.J.; Bessant, C.; Pitzalis, C.; Dalli, J. Blood pro-resolving mediators are linked with synovial pathology and are predictive of DMARD responsiveness in rheumatoid arthritis. Nat. Commun. 2020, 11, 5420. [Google Scholar] [CrossRef]
  41. Barden, A.E.; Moghaddami, M.; Mas, E.; Phillips, M.; Cleland, L.G.; Mori, T.A. Specialised pro-resolving mediators of inflammation in inflammatory arthritis. Prostaglandins Leukot. Essent. Fatty Acids 2016, 107, 24–29. [Google Scholar] [CrossRef]
  42. Freire, M.O.; Dalli, J.; Serhan, C.N.; Van Dyke, T.E. Neutrophil Resolvin E1 Receptor Expression and Function in Type 2 Diabetes. J. Immunol. 2017, 198, 718–728. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Elabdeen, H.R.; Mustafa, M.; Szklenar, M.; Ruhl, R.; Ali, R.; Bolstad, A.I. Ratio of pro-resolving and pro-inflammatory lipid mediator precursors as potential markers for aggressive periodontitis. PLoS ONE 2013, 8, e70838. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Wang, C.W.; Colas, R.A.; Dalli, J.; Arnardottir, H.H.; Nguyen, D.; Hasturk, H.; Chiang, N.; Van Dyke, T.E.; Serhan, C.N. Maresin 1 Biosynthesis and Proresolving Anti-infective Functions with Human-Localized Aggressive Periodontitis Leukocytes. Infect. Immun. 2015, 84, 658–665. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Claria, J.; Serhan, C.N. Aspirin triggers previously undescribed bioactive eicosanoids by human endothelial cell-leukocyte interactions. Proc. Natl. Acad. Sci. USA 1995, 92, 9475–9479. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Gilligan, M.M.; Gartung, A.; Sulciner, M.L.; Norris, P.C.; Sukhatme, V.P.; Bielenberg, D.R.; Huang, S.; Kieran, M.W.; Serhan, C.N.; Panigrahy, D. Aspirin-triggered proresolving mediators stimulate resolution in cancer. Proc. Natl. Acad. Sci. USA 2019, 116, 6292–6297. [Google Scholar] [CrossRef] [Green Version]
  47. Planaguma, A.; Pfeffer, M.A.; Rubin, G.; Croze, R.; Uddin, M.; Serhan, C.N.; Levy, B.D. Lovastatin decreases acute mucosal inflammation via 15-epi-lipoxin A4. Mucosal. Immunol. 2010, 3, 270–279. [Google Scholar] [CrossRef] [Green Version]
  48. Birnbaum, Y.; Ye, Y.; Lin, Y.; Freeberg, S.Y.; Nishi, S.P.; Martinez, J.D.; Huang, M.H.; Uretsky, B.F.; Perez-Polo, J.R. Augmentation of myocardial production of 15-epi-lipoxin-a4 by pioglitazone and atorvastatin in the rat. Circulation 2006, 114, 929–935. [Google Scholar] [CrossRef] [Green Version]
  49. Radmark, O.; Malmsten, C.; Samuelsson, B.; Clark, D.A.; Goto, G.; Marfat, A.; Corey, E.J. Leukotriene A: Stereochemistry and enzymatic conversion to leukotriene B. Biochem. Biophys. Res. Commun. 1980, 92, 954–961. [Google Scholar] [CrossRef]
  50. Pfeiffer, F.; Mayer, G. Selection and Biosensor Application of Aptamers for Small Molecules. Front. Chem. 2016, 4, 25. [Google Scholar] [CrossRef] [Green Version]
  51. Wang, X.; Cohen, L.; Wang, J.; Walt, D.R. Competitive Immunoassays for the Detection of Small Molecules Using Single Molecule Arrays. J. Am. Chem. Soc. 2018, 140, 18132–18139. [Google Scholar] [CrossRef] [PubMed]
  52. Liakh, I.; Pakiet, A.; Sledzinski, T.; Mika, A. Modern Methods of Sample Preparation for the Analysis of Oxylipins in Biological Samples. Molecules 2019, 24, 1639. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Koenis, D.S.; Beegun, I.; Jouvene, C.C.; Aguirre, G.A.; Souza, P.R.; Gonzalez-Nunez, M.; Ly, L.; Pistorius, K.; Kocher, H.M.; Ricketts, W.; et al. Disrupted Resolution Mechanisms Favor Altered Phagocyte Responses in COVID-19. Circ. Res. 2021, 129, e54–e71. [Google Scholar] [CrossRef] [PubMed]
  54. Diaz, L.A.; Altman, N.H.; Khan, W.N.; Serhan, C.N.; Adkins, B. Specialized Proresolving Mediators Rescue Infant Mice from Lethal Citrobacter rodentium Infection and Promote Immunity against Reinfection. Infect. Immun. 2017, 85, e00464-17. [Google Scholar] [CrossRef] [Green Version]
  55. Chiang, N.; Fredman, G.; Backhed, F.; Oh, S.F.; Vickery, T.; Schmidt, B.A.; Serhan, C.N. Infection regulates pro-resolving mediators that lower antibiotic requirements. Nature 2012, 484, 524–528. [Google Scholar] [CrossRef] [Green Version]
  56. Palmer, C.D.; Mancuso, C.J.; Weiss, J.P.; Serhan, C.N.; Guinan, E.C.; Levy, O. 17(R)-Resolvin D1 differentially regulates TLR4-mediated responses of primary human macrophages to purified LPS and live E. coli. J. Leukoc. Biol. 2011, 90, 459–470. [Google Scholar] [CrossRef] [Green Version]
  57. Gu, J.; Luo, L.; Wang, Q.; Yan, S.; Lin, J.; Li, D.; Cao, B.; Mei, H.; Ying, B.; Bin, L.; et al. Maresin 1 attenuates mitochondrial dysfunction through the ALX/cAMP/ROS pathway in the cecal ligation and puncture mouse model and sepsis patients. Lab. Investig. 2018, 98, 715–733. [Google Scholar] [CrossRef]
  58. Motwani, M.P.; Colas, R.A.; George, M.J.; Flint, J.D.; Dalli, J.; Richard-Loendt, A.; De Maeyer, R.P.; Serhan, C.N.; Gilroy, D.W. Pro-resolving mediators promote resolution in a human skin model of UV-killed Escherichia coli-driven acute inflammation. JCI Insight 2018, 3, e94463. [Google Scholar] [CrossRef] [Green Version]
  59. Norris, P.C.; Skulas-Ray, A.C.; Riley, I.; Richter, C.K.; Kris-Etherton, P.M.; Jensen, G.L.; Serhan, C.N.; Maddipati, K.R. Identification of specialized pro-resolving mediator clusters from healthy adults after intravenous low-dose endotoxin and omega-3 supplementation: A methodological validation. Sci. Rep. 2018, 8, 18050. [Google Scholar] [CrossRef] [Green Version]
  60. Dalli, J.; Colas, R.A.; Quintana, C.; Barragan-Bradford, D.; Hurwitz, S.; Levy, B.D.; Choi, A.M.; Serhan, C.N.; Baron, R.M. Human Sepsis Eicosanoid and Proresolving Lipid Mediator Temporal Profiles: Correlations with Survival and Clinical Outcomes. Crit. Care Med. 2017, 45, 58–68. [Google Scholar] [CrossRef]
  61. Colas, R.A.; Nhat, L.T.H.; Thuong, N.T.T.; Gomez, E.A.; Ly, L.; Thanh, H.H.; Mai, N.T.H.; Phu, N.H.; Thwaites, G.E.; Dalli, J. Proresolving mediator profiles in cerebrospinal fluid are linked with disease severity and outcome in adults with tuberculous meningitis. FASEB J. 2019, 33, 13028–13039. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  62. Ramon, S.; Baker, S.F.; Sahler, J.M.; Kim, N.; Feldsott, E.A.; Serhan, C.N.; Martinez-Sobrido, L.; Topham, D.J.; Phipps, R.P. The specialized proresolving mediator 17-HDHA enhances the antibody-mediated immune response against influenza virus: A new class of adjuvant? J. Immunol. 2014, 193, 6031–6040. [Google Scholar] [CrossRef] [PubMed]
  63. Morita, M.; Kuba, K.; Ichikawa, A.; Nakayama, M.; Katahira, J.; Iwamoto, R.; Watanebe, T.; Sakabe, S.; Daidoji, T.; Nakamura, S.; et al. The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza. Cell 2013, 153, 112–125. [Google Scholar] [CrossRef] [Green Version]
  64. Rajasagi, N.K.; Reddy, P.B.; Suryawanshi, A.; Mulik, S.; Gjorstrup, P.; Rouse, B.T. Controlling herpes simplex virus-induced ocular inflammatory lesions with the lipid-derived mediator resolvin E1. J. Immunol. 2011, 186, 1735–1746. [Google Scholar] [CrossRef] [PubMed]
  65. Recchiuti, A.; Patruno, S.; Mattoscio, D.; Isopi, E.; Pomilio, A.; Lamolinara, A.; Iezzi, M.; Pecce, R.; Romano, M. Resolvin D1 and D2 reduce SARS-CoV-2-induced inflammatory responses in cystic fibrosis macrophages. FASEB J. 2021, 35, e21441. [Google Scholar] [CrossRef] [PubMed]
  66. Archambault, A.S.; Zaid, Y.; Rakotoarivelo, V.; Turcotte, C.; Dore, E.; Dubuc, I.; Martin, C.; Flamand, O.; Amar, Y.; Cheikh, A.; et al. High levels of eicosanoids and docosanoids in the lungs of intubated COVID-19 patients. FASEB J. 2021, 35, e21666. [Google Scholar] [CrossRef] [PubMed]
  67. Schwarz, B.; Sharma, L.; Roberts, L.; Peng, X.; Bermejo, S.; Leighton, I.; Casanovas-Massana, A.; Minasyan, M.; Farhadian, S.; Ko, A.I.; et al. Cutting Edge: Severe SARS-CoV-2 Infection in Humans Is Defined by a Shift in the Serum Lipidome, Resulting in Dysregulation of Eicosanoid Immune Mediators. J. Immunol. 2021, 206, 329–334. [Google Scholar] [CrossRef]
  68. Serhan, C.N.; Levy, B.D. Resolvins in inflammation: Emergence of the pro-resolving superfamily of mediators. J. Clin. Investig. 2018, 128, 2657–2669. [Google Scholar] [CrossRef]
  69. Panigrahy, D.; Gilligan, M.M.; Serhan, C.N.; Kashfi, K. Resolution of inflammation: An organizing principle in biology and medicine. Pharmacol. Ther. 2021, 227, 107879. [Google Scholar] [CrossRef]
  70. Carracedo, M.; Artiach, G.; Arnardottir, H.; Back, M. The resolution of inflammation through omega-3 fatty acids in atherosclerosis, intimal hyperplasia, and vascular calcification. Semin. Immunopathol. 2019, 41, 757–766. [Google Scholar] [CrossRef] [Green Version]
  71. Halade, G.V.; Tourki, B. Specialized Pro-resolving Mediators Directs Cardiac Healing and Repair with Activation of Inflammation and Resolution Program in Heart Failure. Adv. Exp. Med. Biol. 2019, 1161, 45–64. [Google Scholar] [CrossRef] [PubMed]
  72. Fredman, G. Can Inflammation-Resolution Provide Clues to Treat Patients According to Their Plaque Phenotype? Front. Pharmacol. 2019, 10, 205. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  73. Chen, G.; Zhang, Y.Q.; Qadri, Y.J.; Serhan, C.N.; Ji, R.R. Microglia in Pain: Detrimental and Protective Roles in Pathogenesis and Resolution of Pain. Neuron 2018, 100, 1292–1311. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  74. Wang, X.; Miao, Z.; Xu, X.; Schultzberg, M.; Zhao, Y. Reduced Levels of Plasma Lipoxin A4 Are Associated with Post-Stroke Cognitive Impairment. J. Alzheimers Dis. 2021, 79, 607–613. [Google Scholar] [CrossRef]
  75. Wang, X.; Zhu, M.; Hjorth, E.; Cortes-Toro, V.; Eyjolfsdottir, H.; Graff, C.; Nennesmo, I.; Palmblad, J.; Eriksdotter, M.; Sambamurti, K.; et al. Resolution of inflammation is altered in Alzheimer’s disease. Alzheimers Dement. 2015, 11, 40–50. [Google Scholar] [CrossRef] [Green Version]
  76. Zhu, M.; Wang, X.; Hjorth, E.; Colas, R.A.; Schroeder, L.; Granholm, A.C.; Serhan, C.N.; Schultzberg, M. Pro-Resolving Lipid Mediators Improve Neuronal Survival and Increase Abeta42 Phagocytosis. Mol. Neurobiol. 2016, 53, 2733–2749. [Google Scholar] [CrossRef]
  77. Fosshaug, L.E.; Colas, R.A.; Anstensrud, A.K.; Gregersen, I.; Nymo, S.; Sagen, E.L.; Michelsen, A.; Vinge, L.E.; Oie, E.; Gullestad, L.; et al. Early increase of specialized pro-resolving lipid mediators in patients with ST-elevation myocardial infarction. EBioMedicine 2019, 46, 264–273. [Google Scholar] [CrossRef]
  78. Halade, G.V.; Kain, V.; Dillion, C.; Beasley, M.; Dudenbostel, T.; Oparil, S.; Limdi, N.A. Race-based and sex-based differences in bioactive lipid mediators after myocar.rdial infarction. ESC Heart Fail. 2020, 7, 1700–1710. [Google Scholar] [CrossRef]
  79. Grenon, S.M.; Owens, C.D.; Nosova, E.V.; Hughes-Fulford, M.; Alley, H.F.; Chong, K.; Perez, S.; Yen, P.K.; Boscardin, J.; Hellmann, J.; et al. Short-Term, High-Dose Fish Oil Supplementation Increases the Production of Omega-3 Fatty Acid-Derived Mediators in Patients With Peripheral Artery Disease (the OMEGA-PAD I Trial). J. Am. Heart Assoc. 2015, 4, e002034. [Google Scholar] [CrossRef] [Green Version]
  80. Schaller, M.S.; Zahner, G.J.; Gasper, W.J.; Harris, W.S.; Conte, M.S.; Hills, N.K.; Grenon, S.M. Relationship between the omega-3 index and specialized pro-resolving lipid mediators in patients with peripheral arterial disease taking fish oil supplements. J. Clin. Lipidol. 2017, 11, 1289–1295. [Google Scholar] [CrossRef]
  81. Fredman, G.; Hellmann, J.; Proto, J.D.; Kuriakose, G.; Colas, R.A.; Dorweiler, B.; Connolly, E.S.; Solomon, R.; Jones, D.M.; Heyer, E.J.; et al. An imbalance between specialized pro-resolving lipid mediators and pro-inflammatory leukotrienes promotes instability of atherosclerotic plaques. Nat. Commun. 2016, 7, 12859. [Google Scholar] [CrossRef] [PubMed]
  82. Tobon-Arroyave, S.I.; Isaza-Guzman, D.M.; Gomez-Ortega, J.; Florez-Alzate, A.A. Salivary levels of specialized pro-resolving lipid mediators as indicators of periodontal health/disease status. J. Clin. Periodontol. 2019, 46, 978–990. [Google Scholar] [CrossRef]
  83. Vickery, T.W.; Armstrong, M.; Kofonow, J.M.; Robertson, C.E.; Kroehl, M.E.; Reisdorph, N.A.; Ramakrishnan, V.R.; Frank, D.N. Altered tissue specialized pro-resolving mediators in chronic rhinosinusitis. Prostaglandins Leukot. Essent. Fatty Acids 2021, 164, 102218. [Google Scholar] [CrossRef] [PubMed]
  84. Shivakoti, R.; Dalli, J.; Kadam, D.; Gaikwad, S.; Barthwal, M.; Colas, R.A.; Mazzacuva, F.; Lokhande, R.; Dharmshale, S.; Bharadwaj, R.; et al. Lipid mediators of inflammation and Resolution in individuals with tuberculosis and tuberculosis-Diabetes. Prostaglandins Other Lipid Mediat. 2020, 147, 106398. [Google Scholar] [CrossRef]
  85. Polus, A.; Zapala, B.; Razny, U.; Gielicz, A.; Kiec-Wilk, B.; Malczewska-Malec, M.; Sanak, M.; Childs, C.E.; Calder, P.C.; Dembinska-Kiec, A. Omega-3 fatty acid supplementation influences the whole blood transcriptome in women with obesity, associated with pro-resolving lipid mediator production. Biochim. Biophys. Acta 2016, 1861, 1746–1755. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  86. Barden, A.; Shinde, S.; Tsai, I.J.; Croft, K.D.; Beilin, L.J.; Puddey, I.B.; Mori, T.A. Effect of weight loss on neutrophil resolvins in the metabolic syndrome. Prostaglandins Leukot. Essent. Fatty Acids 2019, 148, 25–29. [Google Scholar] [CrossRef]
  87. Lopez-Vicario, C.; Titos, E.; Walker, M.E.; Alcaraz-Quiles, J.; Casulleras, M.; Duran-Guell, M.; Flores-Costa, R.; Perez-Romero, N.; Forne, M.; Dalli, J.; et al. Leukocytes from obese individuals exhibit an impaired SPM signature. FASEB J. 2019, 33, 7072–7083. [Google Scholar] [CrossRef] [Green Version]
  88. Schulte, F.; Asbeutah, A.A.; Benotti, P.N.; Wood, G.C.; Still, C.; Bistrian, B.R.; Hardt, M.; Welty, F.K. The relationship between specialized pro-resolving lipid mediators, morbid obesity and weight loss after bariatric surgery. Sci. Rep. 2020, 10, 20128. [Google Scholar] [CrossRef]
  89. Xia, F.; He, C.; Ren, M.; Xu, F.G.; Wan, J.B. Quantitative profiling of eicosanoids derived from n-6 and n-3 polyunsaturated fatty acids by twin derivatization strategy combined with LC-MS/MS in patients with type 2 diabetes mellitus. Anal. Chim. Acta 2020, 1120, 24–35. [Google Scholar] [CrossRef]
  90. Sano, Y.; Toyoshima, S.; Miki, Y.; Taketomi, Y.; Ito, M.; Lee, H.; Saito, S.; Murakami, M.; Okayama, Y. Activation of inflammation and resolution pathways of lipid mediators in synovial fluid from patients with severe rheumatoid arthritis compared with severe osteoarthritis. Asia Pac. Allergy 2020, 10, e21. [Google Scholar] [CrossRef]
  91. Song, J.; Sun, R.; Zhang, Y.; Fu, Y.; Zhao, D. Role of the Specialized Pro-resolving Mediator Resolvin D1 in Hashimoto’s Thyroiditis. Exp. Clin. Endocrinol. Diabetes 2021, 129, 791–797. [Google Scholar] [CrossRef] [PubMed]
  92. See, V.H.L.; Mas, E.; Prescott, S.L.; Beilin, L.J.; Burrows, S.; Barden, A.E.; Huang, R.C.; Mori, T.A. Effects of postnatal omega-3 fatty acid supplementation on offspring pro-resolving mediators of inflammation at 6 months and 5 years of age: A double blind, randomized controlled clinical trial. Prostaglandins Leukot. Essent. Fatty Acids 2017, 126, 126–132. [Google Scholar] [CrossRef] [PubMed]
  93. Lamon-Fava, S.; So, J.; Mischoulon, D.; Ziegler, T.R.; Dunlop, B.W.; Kinkead, B.; Schettler, P.J.; Nierenberg, A.A.; Felger, J.C.; Maddipati, K.R.; et al. Dose- and time-dependent increase in circulating anti-inflammatory and pro-resolving lipid mediators following eicosapentaenoic acid supplementation in patients with major depressive disorder and chronic inflammation. Prostaglandins Leukot. Essent. Fatty Acids 2021, 164, 102219. [Google Scholar] [CrossRef] [PubMed]
  94. So, J.; Wu, D.; Lichtenstein, A.H.; Tai, A.K.; Matthan, N.R.; Maddipati, K.R.; Lamon-Fava, S. EPA and DHA differentially modulate monocyte inflammatory response in subjects with chronic inflammation in part via plasma specialized pro-resolving lipid mediators: A randomized, double-blind, crossover study. Atherosclerosis 2021, 316, 90–98. [Google Scholar] [CrossRef] [PubMed]
  95. Norling, L.V.; Headland, S.E.; Dalli, J.; Arnardottir, H.H.; Haworth, O.; Jones, H.R.; Irimia, D.; Serhan, C.N.; Perretti, M. Proresolving and cartilage-protective actions of resolvin D1 in inflammatory arthritis. JCI Insight 2016, 1, e85922. [Google Scholar] [CrossRef] [PubMed]
  96. Arnardottir, H.H.; Dalli, J.; Norling, L.V.; Colas, R.A.; Perretti, M.; Serhan, C.N. Resolvin D3 Is Dysregulated in Arthritis and Reduces Arthritic Inflammation. J. Immunol. 2016, 197, 2362–2368. [Google Scholar] [CrossRef] [Green Version]
  97. Jin, S.; Chen, H.; Li, Y.; Zhong, H.; Sun, W.; Wang, J.; Zhang, T.; Ma, J.; Yan, S.; Zhang, J.; et al. Maresin 1 improves the Treg/Th17 imbalance in rheumatoid arthritis through miR-21. Ann. Rheum. Dis. 2018, 77, 1644–1652. [Google Scholar] [CrossRef]
  98. Allen, B.L.; Montague-Cardoso, K.; Simeoli, R.; Colas, R.A.; Oggero, S.; Vilar, B.; McNaughton, P.A.; Dalli, J.; Perretti, M.; Sher, E.; et al. Imbalance of proresolving lipid mediators in persistent allodynia dissociated from signs of clinical arthritis. Pain 2020, 161, 2155–2166. [Google Scholar] [CrossRef]
  99. Ozgul Ozdemir, R.B.; Soysal Gunduz, O.; Ozdemir, A.T.; Akgul, O. Low levels of pro-resolving lipid mediators lipoxin-A4, resolvin-D1 and resolvin-E1 in patients with rheumatoid arthritis. Immunol. Lett. 2020, 227, 34–40. [Google Scholar] [CrossRef]
  100. Merched, A.J.; Ko, K.; Gotlinger, K.H.; Serhan, C.N.; Chan, L. Atherosclerosis: Evidence for impairment of resolution of vascular inflammation governed by specific lipid mediators. FASEB J. 2008, 22, 3595–3606. [Google Scholar] [CrossRef]
  101. Ho, K.J.; Spite, M.; Owens, C.D.; Lancero, H.; Kroemer, A.H.; Pande, R.; Creager, M.A.; Serhan, C.N.; Conte, M.S. Aspirin-triggered lipoxin and resolvin E1 modulate vascular smooth muscle phenotype and correlate with peripheral atherosclerosis. Am. J. Pathol. 2010, 177, 2116–2123. [Google Scholar] [CrossRef] [PubMed]
  102. Viola, J.R.; Lemnitzer, P.; Jansen, Y.; Csaba, G.; Winter, C.; Neideck, C.; Silvestre-Roig, C.; Dittmar, G.; Doring, Y.; Drechsler, M.; et al. Resolving Lipid Mediators Maresin 1 and Resolvin D2 Prevent Atheroprogression in Mice. Circ. Res. 2016, 119, 1030–1038. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  103. Colas, R.A.; Souza, P.R.; Walker, M.E.; Burton, M.; Zaslona, Z.; Curtis, A.M.; Marques, R.M.; Dalli, J. Impaired Production and Diurnal Regulation of Vascular RvDn-3 DPA Increase Systemic Inflammation and Cardiovascular Disease. Circ. Res. 2018, 122, 855–863. [Google Scholar] [CrossRef] [PubMed]
  104. Sato, M.; Aoki-Saito, H.; Fukuda, H.; Ikeda, H.; Koga, Y.; Yatomi, M.; Tsurumaki, H.; Maeno, T.; Saito, T.; Nakakura, T.; et al. Resolvin E3 attenuates allergic airway inflammation via the interleukin-23-interleukin-17A pathway. FASEB J. 2019, 33, 12750–12759. [Google Scholar] [CrossRef] [Green Version]
  105. Flesher, R.P.; Herbert, C.; Kumar, R.K. Resolvin E1 promotes resolution of inflammation in a mouse model of an acute exacerbation of allergic asthma. Clin. Sci. 2014, 126, 805–814. [Google Scholar] [CrossRef] [Green Version]
  106. Rogerio, A.P.; Haworth, O.; Croze, R.; Oh, S.F.; Uddin, M.; Carlo, T.; Pfeffer, M.A.; Priluck, R.; Serhan, C.N.; Levy, B.D. Resolvin D1 and aspirin-triggered resolvin D1 promote resolution of allergic airways responses. J. Immunol. 2012, 189, 1983–1991. [Google Scholar] [CrossRef] [Green Version]
  107. Zambalde, E.P.; Teixeira, M.M.; Favarin, D.C.; de Oliveira, J.R.; Magalhaes, M.L.; Cunha, M.M.; Silva, W.C.J.; Okuma, C.H.; Rodrigues, V.J.; Levy, B.D.; et al. The anti-inflammatory and pro-resolution effects of aspirin-triggered RvD1 (AT-RvD1) on peripheral blood mononuclear cells from patients with severe asthma. Int. Immunopharmacol. 2016, 35, 142–148. [Google Scholar] [CrossRef]
  108. Miyata, J.; Fukunaga, K.; Iwamoto, R.; Isobe, Y.; Niimi, K.; Takamiya, R.; Takihara, T.; Tomomatsu, K.; Suzuki, Y.; Oguma, T.; et al. Dysregulated synthesis of protectin D1 in eosinophils from patients with severe asthma. J. Allergy Clin. Immunol. 2013, 131, 353–360. [Google Scholar] [CrossRef]
  109. Hottz, E.D.; Azevedo-Quintanilha, I.G.; Palhinha, L.; Teixeiraet, L.; Barreto, E.A.; Pão, C.R.R.; Righy, C.; Franco, S.; Souza, T.M.L.; Kurtz, P.; et al. Platelet activation and platelet-monocyte aggregate formation trigger tissue factor expression in patients with severe COVID-19. Blood 2020, 136, 1330–1341. [Google Scholar] [CrossRef]
  110. Sun, Y.P.; Oh, S.F.; Uddin, J.; Yang, R.; Gotlinger, K.; Campbell, E.; Colgan, S.P.; Petasis, N.A.; Serhan, C.N. Resolvin D1 and its aspirin-triggered 17R epimer. Stereochemical assignments, anti-inflammatory properties, and enzymatic inactivation. J. Biol. Chem. 2007, 282, 9323–9334. [Google Scholar] [CrossRef] [Green Version]
  111. Chiang, N.; Bermudez, E.A.; Ridker, P.M.; Hurwitz, S.; Serhan, C.N. Aspirin triggers antiinflammatory 15-epi-lipoxin A4 and inhibits thromboxane in a randomized human trial. Proc. Natl. Acad. Sci. USA 2004, 101, 15178–15183. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  112. Chiang, N.; Hurwitz, S.; Ridker, P.M.; Serhan, C.N. Aspirin has a gender-dependent impact on antiinflammatory 15-epi-lipoxin A4 formation: A randomized human trial. Arterioscler. Thromb. Vasc. Biol. 2006, 26, e14–e17. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  113. Bali, S.K.; Madaiah, H.; Dharmapalan, J.; Janarthanam, S.; Tarannum, F. Effect of systemic long-term, low-dose aspirin on periodontal status and soluble CD14 in gingival crevicular fluid: A case-control study. J. Investig. Clin. Dent. 2018, 9, e12353. [Google Scholar] [CrossRef]
  114. Walker, M.E.; Souza, P.R.; Colas, R.A.; Dalli, J. 13-Series resolvins mediate the leukocyte-platelet actions of atorvastatin and pravastatin in inflammatory arthritis. FASEB J. 2017, 31, 3636–3648. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  115. Pyrillou, K.; Chairakaki, A.D.; Tamvakopoulos, C.; Andreakos, E. Dexamethasone induces omega3-derived immunoresolvents driving resolution of allergic airway inflammation. J. Allergy Clin. Immunol. 2018, 142, 691–695. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  116. Abdulrazaq, M.; Innes, J.K.; Calder, P.C. Effect of omega-3 polyunsaturated fatty acids on arthritic pain: A systematic review. Nutrition 2017, 39, 57–66. [Google Scholar] [CrossRef] [PubMed]
  117. Yates, C.M.; Calder, P.C.; Ed Rainger, G. Pharmacology and therapeutics of omega-3 polyunsaturated fatty acids in chronic inflammatory disease. Pharmacol. Ther. 2014, 141, 272–282. [Google Scholar] [CrossRef]
  118. Fiala, M.; Halder, R.C.; Sagong, B.; Ross, O.; Sayre, J.; Porter, V.; Bredesen, D.E. omega-3 Supplementation increases amyloid-beta phagocytosis and resolvin D1 in patients with minor cognitive impairment. FASEB J. 2015, 29, 2681–2689. [Google Scholar] [CrossRef] [Green Version]
  119. Terrando, N.; Gomez-Galan, M.; Yang, T.; Carlstrom, M.; Gustavsson, D.; Harding, R.E.; Lindskog, M.; Eriksson, L.I. Aspirin-triggered resolvin D1 prevents surgery-induced cognitive decline. FASEB J. 2013, 27, 3564–3571. [Google Scholar] [CrossRef] [Green Version]
  120. Barden, A.E.; Shinde, S.; Burke, V.; Puddey, I.B.; Beilin, L.J.; Irish, A.B.; Watts, G.F.; Mori, T.A. The effect of n-3 fatty acids and coenzyme Q10 supplementation on neutrophil leukotrienes, mediators of inflammation resolution and myeloperoxidase in chronic kidney disease. Prostaglandins Other Lipid Mediat. 2018, 136, 1–8. [Google Scholar] [CrossRef]
  121. Souza, P.R.; Marques, R.M.; Gomez, E.A.; Colas, R.A.; De Matteis, R.; Zak, A.; Patel, M.; Collier, D.J.; Dalli, J. Enriched Marine Oil Supplements Increase Peripheral Blood Specialized Pro-Resolving Mediators Concentrations and Reprogram Host Immune Responses: A Randomized Double-Blind Placebo-Controlled Study. Circ. Res. 2020, 126, 75–90. [Google Scholar] [CrossRef] [PubMed]
  122. Claria, J.; Dalli, J.; Yacoubian, S.; Gao, F.; Serhan, C.N. Resolvin D1 and resolvin D2 govern local inflammatory tone in obese fat. J. Immunol. 2012, 189, 2597–2605. [Google Scholar] [CrossRef] [PubMed]
  123. Colas, R.A.; Dalli, J.; Chiang, N.; Vlasakov, I.; Sanger, J.M.; Riley, I.R.; Serhan, C.N. Identification and Actions of the Maresin 1 Metabolome in Infectious Inflammation. J. Immunol. 2016, 197, 4444–4452. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  124. Schaller, M.S.; Chen, M.; Colas, R.A.; Sorrentino, T.A.; Lazar, A.A.; Grenon, S.M.; Dalli, J.; Conte, M.S. Treatment With a Marine Oil Supplement Alters Lipid Mediators and Leukocyte Phenotype in Healthy Patients and Those With Peripheral Artery Disease. J. Am. Heart Assoc. 2020, 9, e016113. [Google Scholar] [CrossRef]
  125. Mamoshina, P.; Volosnikova, M.; Ozerov, I.V.; Putin, E.; Skibina, E.; Cortese, F.; Zhavoronkov, A. Machine Learning on Human Muscle Transcriptomic Data for Biomarker Discovery and Tissue-Specific Drug Target Identification. Front. Genet. 2018, 9, 242. [Google Scholar] [CrossRef]
  126. Bishop, C.M. Pattern Recognition and Machine Learning; Springer: New York, NY, USA, 2006; p. 738. [Google Scholar]
  127. Swan, A.L.; Mobasheri, A.; Allaway, D.; Liddell, S.; Bacardit, J. Application of machine learning to proteomics data: Classification and biomarker identification in postgenomics biology. OMICS 2013, 17, 595–610. [Google Scholar] [CrossRef] [Green Version]
  128. Zitnik, M.; Nguyen, F.; Wang, B.; Leskovec, J.; Goldenberg, A.; Hoffman, M.M. Machine Learning for Integrating Data in Biology and Medicine: Principles, Practice, and Opportunities. Inf. Fusion 2019, 50, 71–91. [Google Scholar] [CrossRef]
  129. Valletta, J.J.; Recker, M. Identification of immune signatures predictive of clinical protection from malaria. PLoS Comput. Biol. 2017, 13, e1005812. [Google Scholar] [CrossRef] [Green Version]
  130. James, G.; Witten, D.; Hastie, T.; Tibshirani, R. An Introduction to Statistical Learning: With Applications in R, 6th ed.; Springer: Berlin/Heidelberg, Germany, 2013; p. 426. [Google Scholar]
  131. Maglogiannis, I.G. Emerging Artificial Intelligence Applications in Computer Engineering: Real Word AI Systems with Applications in eHealth, HCI, Information Retrieval and Pervasive Technologies; IOS Press: Washington, DC, USA, 2007; p. 407. [Google Scholar]
  132. Bennett, K.P.; Campbell, C. Support vector machines. ACM SIGKDD Explor. Newsletter 2000, 2, 1–13. [Google Scholar] [CrossRef]
  133. Gordon, A.D.; Breiman, L.; Friedman, J.H.; Olshen, R.A.; Stone, C.J. Classification and Regression Trees. Biometrics 1984, 40, 14–23. [Google Scholar] [CrossRef] [Green Version]
  134. Rathod, K.S.; Kapil, V.; Velmurugan, S.; Khambata, R.S.; Siddique, U.; Khan, S.; Van Eijl, S.; Gee, L.C.; Bansal, J.; Pitrola, K.; et al. Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans. J. Clin. Investig. 2017, 127, 169–182. [Google Scholar] [CrossRef] [PubMed]
  135. Troisi, F.; Pace, S.; Jordan, P.M.; Meyer, K.P.L.; Bilancia, R.; Ialenti, A.; Borrelli, F.; Rossi, A.; Sautebin, L.; Serhan, C.N.; et al. Sex Hormone-Dependent Lipid Mediator Formation in Male and Female Mice During Peritonitis. Front. Pharmacol. 2021, 12, 818544. [Google Scholar] [CrossRef] [PubMed]
Biomolecules 12 00353 g001 550
Figure 1. Role of lipid mediators in the initiation and resolution of inflammation.
Figure 1. Role of lipid mediators in the initiation and resolution of inflammation.
Biomolecules 12 00353 g001
Biomolecules 12 00353 g002 550
Figure 2. Separation of specialized pro-resolving mediators using Reverse Phase High Pressure Liquid Chromatography. Representative examples of (A) the chromatographic separation of DHA derived D-series resolvins demonstrating the separation of both di- and tri-hydroxylated species as well as positional isomers and (B) DHA-derived Protectins (PD) demonstrating the chromatographic separation of double bond and chiral isomers using RP-HPLC. Conditions employed in the separation and identification of these autacoids are as follows: samples were injected using a Shimadzu LC-20AD HPLC and a Shimadzu SIL-20AC autoinjector, paired with a QTrap 6500+ (Sciex). An Agilent Poroshell 120 EC-C18 column (100 mm × 4.6 mm × 2.7 µm) was kept at 50 °C and mediators eluted using a mobile phase consisting of methanol/water/acetic acid of 20:80:0.01 (v/v/v) that was ramped to 50:50:0.01 (v/v/v) over 0.5 min, then to 80:20:0.01 (v/v/v) from 2 min to 11 min, maintained until 14.5 min and then rapidly ramped to 98:2:0.01 (v/v/v) for the next 0.1 min. This was subsequently maintained at 98:2:0.01 (v/v/v) for 5.4 min, and the flow rate was maintained at 0.5 mL/min. For identification of the mediators, the QTRap 6500+ was operated in negative ion mode using a multiple reaction monitoring method with the following transitions: RvD1: m/z 375 > 141, RvD2: m/z 375 > 215, RvD3; m/z 375 > 147; RvD4: m/z 375 > 101; RvD5: m/z 359 > 199; PD1: m/z 359 > 153; 10S, 17S-EZE-diHDHA: m/z 359 > 153; 10S, 17S-EEE-diHDHA: m/z 359 > 153; 17R-PD1/AT-PD1: m/z 359 > 153.
Figure 2. Separation of specialized pro-resolving mediators using Reverse Phase High Pressure Liquid Chromatography. Representative examples of (A) the chromatographic separation of DHA derived D-series resolvins demonstrating the separation of both di- and tri-hydroxylated species as well as positional isomers and (B) DHA-derived Protectins (PD) demonstrating the chromatographic separation of double bond and chiral isomers using RP-HPLC. Conditions employed in the separation and identification of these autacoids are as follows: samples were injected using a Shimadzu LC-20AD HPLC and a Shimadzu SIL-20AC autoinjector, paired with a QTrap 6500+ (Sciex). An Agilent Poroshell 120 EC-C18 column (100 mm × 4.6 mm × 2.7 µm) was kept at 50 °C and mediators eluted using a mobile phase consisting of methanol/water/acetic acid of 20:80:0.01 (v/v/v) that was ramped to 50:50:0.01 (v/v/v) over 0.5 min, then to 80:20:0.01 (v/v/v) from 2 min to 11 min, maintained until 14.5 min and then rapidly ramped to 98:2:0.01 (v/v/v) for the next 0.1 min. This was subsequently maintained at 98:2:0.01 (v/v/v) for 5.4 min, and the flow rate was maintained at 0.5 mL/min. For identification of the mediators, the QTRap 6500+ was operated in negative ion mode using a multiple reaction monitoring method with the following transitions: RvD1: m/z 375 > 141, RvD2: m/z 375 > 215, RvD3; m/z 375 > 147; RvD4: m/z 375 > 101; RvD5: m/z 359 > 199; PD1: m/z 359 > 153; 10S, 17S-EZE-diHDHA: m/z 359 > 153; 10S, 17S-EEE-diHDHA: m/z 359 > 153; 17R-PD1/AT-PD1: m/z 359 > 153.
Biomolecules 12 00353 g002
Biomolecules 12 00353 g003 550
Figure 3. Specialized pro-resolving mediators mediate the protective activities of statins and aspirin. Mounting evidence suggests that SPM are central in mediating the protective activates of widely used drugs such as statins and aspirin. This figure provides a summary of the lipid mediator families that have been shown to mediate the anti-inflammatory activities of specific drugs.
Figure 3. Specialized pro-resolving mediators mediate the protective activities of statins and aspirin. Mounting evidence suggests that SPM are central in mediating the protective activates of widely used drugs such as statins and aspirin. This figure provides a summary of the lipid mediator families that have been shown to mediate the anti-inflammatory activities of specific drugs.
Biomolecules 12 00353 g003
Biomolecules 12 00353 g004 550
Figure 4. Schematic diagram of machine learning strategy. This schematic provides an overview of the distinct steps that are required for leveraging machine learning strategies for both the identification of candidate molecules and for their validation as biomarkers. The first step involves the assembly of a training dataset that contains concentrations of SPM in a cohort of patients with distinct outcomes for which a biomarker is sought. This dataset should contain a sufficient number of samples to allow for both the establishment of the dataset to be used to create the predictive models (using different machine learning strategies such us random forest) and the testing dataset. The latter should be employed to calculate how good the model is at predicting between conditions. During the validation step, the training and testing dataset are reshuffled several times using either cross-validation or bootstrapping (reshuffle with replacement) with the purpose of identifying the best predictive model and avoiding overfitting. When validation is completed, and the sensitivity and specificity of the model are calculated, biomarkers are identified, and the model can be evaluated using a new dataset. The evaluation of the model in an independent dataset is the most important step of the process since it confirms that the created model is sufficiently robust in predicting outcomes. Receiver operating characteristic (ROC) curves, among other accuracy tests, are used to evaluate the performance of machine learning models.
Figure 4. Schematic diagram of machine learning strategy. This schematic provides an overview of the distinct steps that are required for leveraging machine learning strategies for both the identification of candidate molecules and for their validation as biomarkers. The first step involves the assembly of a training dataset that contains concentrations of SPM in a cohort of patients with distinct outcomes for which a biomarker is sought. This dataset should contain a sufficient number of samples to allow for both the establishment of the dataset to be used to create the predictive models (using different machine learning strategies such us random forest) and the testing dataset. The latter should be employed to calculate how good the model is at predicting between conditions. During the validation step, the training and testing dataset are reshuffled several times using either cross-validation or bootstrapping (reshuffle with replacement) with the purpose of identifying the best predictive model and avoiding overfitting. When validation is completed, and the sensitivity and specificity of the model are calculated, biomarkers are identified, and the model can be evaluated using a new dataset. The evaluation of the model in an independent dataset is the most important step of the process since it confirms that the created model is sufficiently robust in predicting outcomes. Receiver operating characteristic (ROC) curves, among other accuracy tests, are used to evaluate the performance of machine learning models.
Biomolecules 12 00353 g004
Table
Table 1. Specialized pro-resolving mediator families and stereochemistries. This table provides a summary of the complete stereochemistries, where established, for the SPM distinct as subdivided by families and the essential fatty acid substrate from which they are derived. It also provides the public database reference numbers for each of these molecules where these are available.
Table 1. Specialized pro-resolving mediator families and stereochemistries. This table provides a summary of the complete stereochemistries, where established, for the SPM distinct as subdivided by families and the essential fatty acid substrate from which they are derived. It also provides the public database reference numbers for each of these molecules where these are available.
SubstrateSPM FamiliesAbbreviationChemical NameLipid Maps LM IDPubChem CID
DHAD-series ResolvinsRvD17S,8R,17S-trihydroxy-4Z,9E,11E,13Z,15E,19Z-docosahexaenoic acid LMFA0403001144251266
RvD27S,16R,17S-trihydroxy-4Z,8E,10Z,12E,14E,19Z-docosahexaenoic acid LMFA0403000111383310
RvD34S,11R,17S-trihydroxy-5Z,7E,9E,13Z,15E,19Z-docosahexaenoic acid LMFA0403001271665428
RvD44S,5R,17S-trihydroxydocosa-6E,8E,10Z,13Z,15E,19Z hexaenoic acid LMFA0403000216061138
RvD57S,17S-dihydroxy-4Z,8E,10Z,13Z,15Z,19E-docosahexaenoic acid LMFA0403000316061139
RvD64S,17S-dihydroxy-5E,7E,10Z,13Z,15E,19Z-docosahexaenoic acid LMFA0403000425073193
17R-RvD17S,8R,17R-trihydroxy-4Z,9E,11E,13Z,15E,19Z-docosahexaenoic acid
17R-RvD34S,11R,17R-trihydroxy-5Z,7E,9E,13Z,15E,19Z-docosahexaenoic acid
ProtectinsNPD1/PD110R,17S-dihydroxy-4Z,7Z,11E,13E,15Z,19Z- docosahexaenoic acid LMFA0404000116042541
PDX10S,17S-dihydroxy-4Z,7Z,11E,13Z,15E,19Z-docosahexaenoic acidLMFA0404000311667655
17R-PD110R,17R-dihydroxy-4Z,7Z,11E,13E,15Z,19Z-docosahexaenoic acid 132282528
22-OH-PD110R,17S,22-trihydroxy-4Z,7Z,11E,13E,15Z,19Z-docosahexaenoic acid 132472333
cys-ProtectinsPCTR116R-glutathionyl-17S-hydroxy-4Z,7Z,10Z,12E,14E,19Z-docosahexaenoic acid LMFA04040004132472316
PCTR216R-cysteinylglycinyl-17S-hydroxy-4Z,7Z,10Z,12E,14E,19Z-docosahexaenoic acid LMFA04040005132472317
PCTR316R-cysteinyl-17S-hydroxy-4Z,7Z,10Z,12E,14E,19Z-docosahexaenoic acid LMFA04040006132472318
MaresinsMaR17R,14S-dihydroxy-4Z,8E,10E,12Z,16Z,19Z- docosahexaenoic acid LMFA0405000160201795
MaR213R,14S-dihydroxy-4Z,7Z,9E,11E,16Z,19Z- docosahexaenoic acid LMFA04050004101894912
cys-MaresinsMCTR113R-glutathionyl-14S-hydroxy-4Z,7Z,9E,11E,16Z,19Z-docosahexaenoic acid LMFA04050005122368871
MCTR213R-cysteinylglycinyl-14S-hydroxy-4Z,7Z,9E,11E,16Z,19Z-docosahexaenoic acid LMFA04050006122368872
MCTR313R-cysteinyl-14S-hydroxy-4Z,7Z,9E,11E,16Z,19Z-docosahexaenoic acid LMFA04050007122368873
n-3 DPA13-series ResolvinsRvT17S,13R,20S-trihydroxy-8E,10Z,14E,16Z,18E-docosapentaenoic acid LMFA04000091124202379
RvT27,12,13-trihydroxy-8,10,14,16,19-docosapentaenoic acid LMFA04000092124202381
RvT37,8,13-trihydroxy-9,11,14,16,19-docosapentaenoic acid LMFA04000093124202383
RvT47S,13R-dihydroxy-8E,10Z,14E,16Z,19Z-docosapentaenoic acid LMFA04000094124202385
D-seriesRvD1n-3 DPA7S,8R,17S-trihydroxy-9E,11E,13Z,15E,19Z-docosapentaenoic acid 132472356
RvD2n-3 DPA7,16,17-trihydroxy-8,10,12,14,19-docosapentaenoic acid 132472324
RvD5n-3 DPA7S,17S-dihydroxy-8E,10Z,13Z,15Z,19E-docosapentaenoic acid 132472358
ProtectinsPD1n-3 DPA10R,17S-dihydroxy-7Z,11E,13E,15Z,19Z-docosapentaenoic acidLMFA04000096132472351
PD2n-3 DPA16,17-dihydroxy-7Z,10,13,14,19-docosapentaenoic acidLMFA04000097132472319
MaresinsMaR1n-3 DPA7R,14S-dihydroxy-8E,10E,12Z,16Z,19Z-docosapentaenoic acid
MaR2n-3 DPA13,14-dihydroxy-7,9,11,16,19-docosapentaenoic acid
EPAE-series ResolvinRvE15S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid LMFA0314000310473088
RvE25S,18R-dihydroxy-6E,8Z,11Z,14Z,16E-eicosapentaenoic acid LMFA0314001116061125
RvE317R,18R-dihydroxy-5Z,8Z,11Z,13E,15E-eicosapentaenoic acid LMFA0314000660150429
RvE45S,15S-dihydroxy-6E,8Z,11Z,13E,17Z-eicosapentaenoic acid
AALipoxinsLXA45S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acidLMFA030400015280914
LXB45S,14R,15S-trihydroxy-6E,8Z,10E,12E-eicosatetraenoic acidLMFA030400025280915
15-epi-LXA45S,6R,15R-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acidLMFA030400109841438
15-epi-LXB45S,14R,15R-trihydroxy-6E,8Z,10E,12E-eicosatetraenoic acidLMFA0304000770678885
Table
Table 2. Examples of specialized pro-resolving mediators identification in tissues from healthy volunteers and patients with inflammatory disorders.
Table 2. Examples of specialized pro-resolving mediators identification in tissues from healthy volunteers and patients with inflammatory disorders.
DiseaseSPM IdentifiedTissueMethodLC Solvent SystemMS/MS Ionization ModeReferences
Neuronal InflammationIschemic brain injuryLXA4PlasmaELISAN/AN/A [74]
Alzheimer’s diseaseLXA4, RvD1, MaR1, 5,15-diHETECSF, HippocampusELISA, LC-MS/MSmethanol/water/acetic acidNegative [75]
RvD5, PD1, MaR1, Entorhinal cortex tissueLC-MS/MSmethanol/water/acetic acidNegative [76]
Cardiovascular diseasesMyocardial infarctionPD1, 10S,17S-diHDHA, AT-PD1, LXA4, AT-LXA4, RvD5n-3 DPA, PD2n-3 DPA, 10S,17S-diHDPAPlasmaLC-MS/MSmethanol/water/acetic acidNegative [77]
PD1, RvE1PlasmaLC-MS/MSFormic acid/water/acetonitrile Negative [78]
Peripheral Artery Disease LXA4PlasmaLC-MS/MSmethanol/water/acetic acidNegative [79]
10,17-diHDHAPlasmaLC-MS/MSmethanol/water/acetic acidNegative [80]
AtherosclerosisRvD1, 7-epi,Δ12-trans-MaR1, Human carotid atherosclerotic plaquesLC-MS/MSmethanol/water/acetic acidNegative [81]
InfectionsInfection, low-dose endotoxinRvD1, RvE1Plasma and serumLC-MS/MSmethanol/water/acetic acid and acetonitrileNegative [59]
Periodontal diseasePD1, MaR1, LXA4SalivaELISAN/AN/A [82]
RhinosinusitisRvD1, RvD2, LXA4Ethmoid sinus tissueLC-MS/MSFormic acid/water/acetonitrile Negative [83]
TuberculosisRvD2, 17R-PD1, PCTR3, PD1n-3 DPA, RvE3, 15-oxo-LXA4SerumLC-MS/MSmethanol/water/acetic acidPositive [84]
SepsisRvD5, 17R-RvD1, PD1, 17R-PD1, RvE1, RvE2, RvE3, 5S,15S-diHETE, PlasmaLC-MS/MSmethanol/water/acetic acidNegative [60]
Metabolic diseaseObesityRvD1, RvD2, 10S,17S-diHDHA, LXA4, LXA5PlasmaELISAN/AN/A [85]
RvE1Ex vivo neutrophil stimulationLC-MS/MSmethanol/water/acetic acidNegative [86]
RvD2, RvD4, LXA4, RvE3PlasmaLC-MS/MSmethanol/water/acetic acidNegative [87]
RvD3, RvD4, PD1, MaR1SerumLC-MS/MSmethanol/water/acetic acidNegative [88]
DiabetesRvD1, RvE1PlasmaLC-MS/MSFormic acid/water/acetonitrile Positive [89]
RvD2, 17R-PD1, PCTR3, PD1n-3 DPA, RvE3, 15-oxo-LXA4SerumLC-MS/MSmethanol/water/acetic acidPositive [84]
Autoimmune diseaseRheumatoid ArthritisRvD1, 17R-RvD1, RvD2, PD1, 10S,17S-diHDHA, MaR1, RvE1, RvE2, RvE3, 18R-RvE3Plasma and synovial fluidLC-MS/MSammonium acetate/methanolNegative [41]
RvD1, RvD2, RvD3, RvD4, 10S,17S-diHDHA, 17R-PD1, MaR1, 4S,14S-diHDHA, 10S,17S-diHDPA, MaR1n-3 DPA, 15R-LXA4,PlasmaLC-MS/MSmethanol/water/acetic acidNegative [40]
PD1, LXA4, LXB4Synovial fluidLC-MS/MSacetonitrile/methanol/waterNegative [90]
OsteoarthritisPD1, LXA4, LXB4Synovial fluidLC-MS/MSacetonitrile/methanol/waterNegative [90]
Hashimoto’s ThyroiditisRvD1SerumELISA [91]
Omega-3 SupplementationHealthy volunteersRvD1, 17R-RvD1, RvD2, RvE1, RvE2, RvE3, 18R-RvE3PlasmaLC-MS/MSmethanol/water/acetic acidNegative [92]
Healthy volunteers and patients with periphery artery diseaseLXA4PlasmaLC-MS/MSmethanol/water/acetic acidNegative [79]
Periphery artery diseasePDX (10S,17S-diHDHA)PlasmaLC-MS/MSmethanol/water/acetic acidNegative [80]
Major depressive disorder and chronic inflammationRvE2, RvE3, LXB4PlasmaLC-MS/MSacetonitrile/methanol/waterNegative [93]
Chronic inflammationRvD5n-3 DPA, MaR1n-3 DPAPlasmaLC-MS/MSacetonitrile/methanol/waterNegative [94]
Lipid mediators denoted in red were observed to be decreased whereas those denoted in blue were observed to be increased in comparison to the respective in-study control group. (n/a = not applicable).
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Dalli, J.; Gomez, E.A.; Jouvene, C.C. Utility of the Specialized Pro-Resolving Mediators as Diagnostic and Prognostic Biomarkers in Disease. Biomolecules 2022, 12, 353. https://doi.org/10.3390/biom12030353

AMA Style

Dalli J, Gomez EA, Jouvene CC. Utility of the Specialized Pro-Resolving Mediators as Diagnostic and Prognostic Biomarkers in Disease. Biomolecules. 2022; 12(3):353. https://doi.org/10.3390/biom12030353

Chicago/Turabian Style

Dalli, Jesmond, Esteban Alberto Gomez, and Charlotte Camille Jouvene. 2022. "Utility of the Specialized Pro-Resolving Mediators as Diagnostic and Prognostic Biomarkers in Disease" Biomolecules 12, no. 3: 353. https://doi.org/10.3390/biom12030353

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop