Bioactive Compounds from Endophytic Bacteria Bacillus subtilis Strain EP1 with Their Antibacterial Activities
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Isolation and Purification
2.2. DNA Extraction, Sequencing, and Identification
2.3. Extraction of Biomass
2.4. Extraction and Isolation of Pure Compounds
2.5. Antibacterial Activities
2.6. Molecular Validation of the Antibacterial Activities
2.6.1. RNA Extraction
2.6.2. cDNA Synthesis and QRT PCR Analysis
3. Results and Discussion
3.1. Strain Identification
3.2. Characterization of Compounds through NMR and Mass Spectroscopy
3.3. Antibacterial Significance
3.4. Beta-Lactam Gene Inhibition
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Beta-Lactam Biosynthesis | |||
---|---|---|---|
Gene Name | Code | F | Reference |
δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine synthetase | ACVS F | AGCCGTGAAAAGCCACTTGA | [39] |
ACVS R | GCTCACTGATGCCAATGGTTC | ||
aminoadipate aminotransferase | AADAT F | GTGGCATCGGTAGAGAAGCATT | |
AADAT R | CAACGTGAAGCATTCTCATCCA | ||
Ubiquitin | UBQ F | CAATCACCTTGGAAGTCGAGTCGTC | [40] |
UBQ R | CTGGATGTTGTAGTCGGAAAGGGTG |
1 | 2 | 3 | 4 | |
---|---|---|---|---|
Concentration | (Mean ± SD in mm) | |||
Amoxicillin | 24 ± 1 | 23 ± 1 | 20 ± 1 | 26 ± 1 |
1 mg/mL | 11 ± 1 | 10 ± 1 | 11 ± 0.5 | 13 ± 1 |
500 µg/mL | 9 ± 1 | 10 ± 0.5 | 10 ± 0.5 | 11 ± 0.5 |
250 µg/mL | 9 ± 1 | 9 ± 0.5 | 9 ± 0.5 | 10 ± 0.5 |
100 µg/mL | 7 ± 0.5 | 9 ± 1 | 10 ± 0.5 | 9 ± 0.5 |
50 µg/mL | 8 ± 1 | 8 ± 0.5 | 8 ± 0.5 | 9 ± 0.5 |
10 µg/mL | 7 ± 1 | 7 ± 0.5 | 7 ± 0.5 | 8 ± 0.5 |
DMSO | 6 ± 0.5 | 6 ± 0.5 | 6 ± 0.5 | 6 ± 0.5 |
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Numan, M.; Shah, M.; Asaf, S.; Ur Rehman, N.; Al-Harrasi, A. Bioactive Compounds from Endophytic Bacteria Bacillus subtilis Strain EP1 with Their Antibacterial Activities. Metabolites 2022, 12, 1228. https://doi.org/10.3390/metabo12121228
Numan M, Shah M, Asaf S, Ur Rehman N, Al-Harrasi A. Bioactive Compounds from Endophytic Bacteria Bacillus subtilis Strain EP1 with Their Antibacterial Activities. Metabolites. 2022; 12(12):1228. https://doi.org/10.3390/metabo12121228
Chicago/Turabian StyleNuman, Muhammad, Muddaser Shah, Sajjad Asaf, Najeeb Ur Rehman, and Ahmed Al-Harrasi. 2022. "Bioactive Compounds from Endophytic Bacteria Bacillus subtilis Strain EP1 with Their Antibacterial Activities" Metabolites 12, no. 12: 1228. https://doi.org/10.3390/metabo12121228