Analyzing the Metabolomic Profile of Yellowtail (Seriola quinquerdiata) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin
Abstract
:1. Introduction
2. Results and Discussion
2.1. Metabolomics Profile
2.2. Differences in Organic Acid Metabolites in the Tricarboxylic Acid (TCA) Cycle
2.3. Differences in Metabolites in Amino Acid Metabolism
2.4. Differences in Metabolites in Other Quantified Compounds
3. Materials and Methods
3.1. Sample Collection and Preparation
3.2. Metabolite Extraction
3.3. CE-TOF/MS Analysis
3.4. Metabolomics Profile Analysis
3.5. Statistics Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Concentration (nmol·g−1) | Comparative Analysis | |||
---|---|---|---|---|
SQK | SQJ | Ratio 2 | p-Value 3 | |
Guanosine | 10 ± 2 1 | 22 ± 6.7 | 2.2 | 0.00023 *** |
Hypoxanthine | 55 ± 22 | 120 ± 79 | 2.2 | 0.031 * |
Guanine | 1.0 ± 0.2 | 2.9 ± 1.6 | 2.8 | 0.006 ** |
Sarcosine | 3.5 ± 1.2 | 13 ± 4.0 | 3.6 | 0.000028 *** |
SQK | SQJ | |||
---|---|---|---|---|
Sample Number | Full Length (cm) | Weight (kg) | Full Length (cm) | Weight (kg) |
1 | 84.0 | 4.92 | 74.0 | 4.46 |
2 | 66.5 | 3.95 | 78.2 | 4.94 |
3 | 71.1 | 3.99 | 75.0 | 4.85 |
4 | 72.3 | 4.15 | 75.6 | 4.10 |
5 | 71.9 | 4.12 | 79.0 | 5.50 |
6 | 70.9 | 3.93 | 80.8 | 5.40 |
7 | 71.8 | 4.14 | 77.0 | 4.97 |
8 | 68.0 | 3.86 | 76.4 | 4.58 |
9 | 72.6 | 4.06 | 77.0 | 3.98 |
10 | 72.7 | 4.56 | 73.8 | 4.07 |
Mean ± S.D. | 72.7 ± 4.63 | 4.2 ± 0.33 | 76.7 ± 2.22 | 4.7 ± 0.54 |
Cationic Metabolites (Cation Mode) | Anionic Metabolites (Anion Mode) | |
---|---|---|
Capillary | fused silica capillary of 50 μm × 80 cm | |
Run buffer | Cation Buffer Soln. (p/n: H3301-1001) | Anion Buffer Soln. (p/n: I3302-1023) |
Rinse buffer | Cation Buffer Soln. (p/n: H3301-1001) | Anion buffer Soln. (p/n: I3302-1023) |
Sample injection | Pressure injection 55 mbar, 10 s | Pressure injection 50 mbar, 22 s |
CE voltage | Positive, 30 kV | Positive, 30 kV |
MS ionization | Electrospray ionization (ESI) Positive | ESI negative |
MS Capillary voltage | 4000 V | 3500 V |
MS scan range | m/z 50–1000 | m/z 50–1000 |
Sheath liquid | HMT Sheath Liquid (p/n: H3301-1021) | HMT Sheath Liquid (p/n: H3301-1021) |
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Shin, J.; Yang, J.; Cha, E.; Kim, H.; Lee, Y.; Kim, S.; Choi, I.; Yang, J. Analyzing the Metabolomic Profile of Yellowtail (Seriola quinquerdiata) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin. Metabolites 2021, 11, 793. https://doi.org/10.3390/metabo11110793
Shin J, Yang J, Cha E, Kim H, Lee Y, Kim S, Choi I, Yang J. Analyzing the Metabolomic Profile of Yellowtail (Seriola quinquerdiata) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin. Metabolites. 2021; 11(11):793. https://doi.org/10.3390/metabo11110793
Chicago/Turabian StyleShin, Jiyoung, Junho Yang, Eunji Cha, Hyunsuk Kim, Yoonhyeung Lee, Soi Kim, Iseul Choi, and Jiyoung Yang. 2021. "Analyzing the Metabolomic Profile of Yellowtail (Seriola quinquerdiata) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin" Metabolites 11, no. 11: 793. https://doi.org/10.3390/metabo11110793