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Review

A Review of Histocytological Events and Molecular Mechanisms Involved in Intestine Regeneration in Holothurians

by
Fang Su
1,2,3,4,5,6,
Hongsheng Yang
1,2,3,4,5,6,7 and
Lina Sun
1,2,3,4,5,6,*
1
CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
3
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
4
CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
5
University of Chinese Academy of Sciences, Beijing 100049, China
6
Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao 266071, China
7
The Innovation of Seed Design, Chinese Academy of Sciences, Wuhan 430071, China
*
Author to whom correspondence should be addressed.
Biology 2022, 11(8), 1095; https://doi.org/10.3390/biology11081095
Submission received: 30 May 2022 / Revised: 21 June 2022 / Accepted: 28 June 2022 / Published: 22 July 2022
(This article belongs to the Special Issue Molecular Physiology of Marine Invertebrates)
Browse Figure
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Abstract

:

Simple Summary

Many species of sea cucumber in Echinodermata may eviscerate most of or even all internal organs when encountering predators or adverse environments, and can achieve regeneration within a certain time. Although regeneration time varies, the mechanism is common. This paper reviewed the intestinal regeneration process of sea cucumbers from the perspectives of histocytology and molecular mechanism. Echinodermata has a special evolutionary position between achordate and chordate, so we hope to explore the common regeneration conserved signals between invertebrates and vertebrates by recording the intestinal regeneration of sea cucumbers.

Abstract

Most species of the class Holothuroidea are able to regenerate most of their internal organs following a typical evisceration process, which is a unique mechanism that allows sea cucumbers to survive in adverse environments. In this review, we compare autotomy among different type of sea cucumber and summarize the histocytological events that occur during the five stages of intestinal regeneration. Multiple cytological activities, such as apoptosis and dedifferentiation, take place during wound healing and anlage formation. Many studies have focused on the molecular regulation mechanisms that underlie regeneration, and herein we describe the techniques that have been used as well as the development-related signaling pathways and key genes that are significantly expressed during intestinal regeneration. Future analyses of the underlying mechanisms responsible for intestinal regeneration should include mapping at the single-cell level. Studies of visceral regeneration in echinoderms provide a unique perspective for understanding whole-body regeneration or appendage regeneration.

1. Introduction

Regeneration, defined as the replacement of damaged or lost parts following injury without scarring or loss of functionality, is a widespread phenomenon in almost all Metazoans [1,2]. For example, planarians have a rich library of adult stem cells, and even when cut into 279 parts, they were still able to regenerate a complete individual [3]. Hydra stem cells also have unlimited self-renewal ability [4]. Most vertebrates can non-uniformly regenerate some tissues or organs after injury [5]. Teleosts have a strong ability to regenerate various tissues and organs, including scales, muscles, fins, spinal cords, and hearts [6]. Urodele amphibians, such as newts and axolotls, can regenerate jaws, tails, limbs, and lenses [7,8,9], and reptiles can re-form appendages [10]. Mammals rely on undifferentiated adult stem cells or dedifferentiation of fully differentiated cells for local regeneration [11]. As the evolutionary status of organisms increases, the regeneration potential is gradually limited.
All classes of echinoderms (Echinoidea (sea urchins and sand dollars), Ophiuroidea (brittle stars), Asteroidea (sea stars), Crinoidea (sea lilies and feather stars), and Holothurioidea (sea cucumbers)) have the capacity to regenerate missing body parts [12]. Sea stars are well-known for regenerating arms [13]. Huet et al. [14,15] studied the ultrastructure and development of the neuroepithelial cells as well as the role of the nervous system during arm regeneration. In addition to adult appendage regeneration, sea star larvae can rapidly and completely regenerate to form fully functional individuals after bisection [16]. Arm regeneration is also a common phenomenon in crinoids and ophiuroids, both of which have long and fragile arms that can be lost to predation or physical stress [12,17]. In addition to arms, they can regenerate internal organs such as the pyloric caeca, cardiac stomach, gonads, and gut [12]. Compared to the other groups, the need for regeneration of sea urchins is reduced and they can regenerate external appendages (spines, pedicellariae), test, and tube feet [12,18]. In contrast, sea cucumbers can regenerate almost all of their viscera.
In 1930, the Italian scholar Bertolini studied the histological characteristics of the regenerating sea cucumber digestive tract and identified various tissues involved in the regeneration process for the first time [19] and published the research results [20]. The pattern of evisceration and regeneration and the duration of regeneration vary among sea cucumber species. For example, in Apostichopus japonicus, the newborn intestine recovers digestive function in 15 days and returns to the normal physiological state in 45 days [21]. The digestive tract of Holothuria scabra regrows within 1 week [22], whereas the new intestine of Holothuria glaberrima returns to its original shape ~1 month after evisceration [23].
In this review, we outline the structure of the digestive tract of sea cucumbers, describe the mode and process of evisceration, assess research methodologies, and progress in our understanding of intestine regeneration at the histological, cytological, and molecular levels.

2. Autotomy and Evisceration

Autotomy (self-cutting) refers to the adaptive detachment of animal body parts as the body’s self-defense mechanism, and it is regulated by nerves [24,25,26]. Many sea cucumber species of the orders Holothuriida, Synallactida, and Dendrochirotida are capable of eviscerating their intestine in response to stressful stimuli, such as crowding, excessive ambient temperature, hypoxia, or encountering predators [27,28]. However, strong stimuli do not always induce autotomy [29]. In addition, some species, such as Eupentacta quinquesemita [30] and Parastichopus californicus [31], undergo seasonal evisceration. During evisceration, holothurians detach most of the viscera (e.g., intestine, respiratory trees, gonads) automatically under the control of nerves [32] and then eject them from the coelom. Subsequently, the tissues regrow rapidly (Table 1).
Some dendrochirotids, such as Cladolabes schmeltzii and Colochirus robustus, lack the ability to eviscerate [39]. Almost all regenerative species of dendrochirotida eviscerate anteriorly. However, Pseudocolochirus violaceus [39] eviscerates through the cloaca. Holothuriida and synallactida generally eviscerate through the cloaca, although some eviscerate by tearing the body wall [39]. Species of Holothuria retain a single respiratory tree in vivo, on either the left or right side [19]. Whether the gonads are eviscerated depends on the reproductive stage.
Evisceration in sea cucumbers occurs either from the rupture of the anterior end of the body (anterior) or the cloaca (posterior) [26,33]. Anterior evisceration, which is most common in Dentrochirotida, results in the loss of almost all of the viscera, including the feeding tentacles, aquapharyngeal bulb, the whole digestive tract and attached haemal vessels, a respiratory tree, and possibly the gonads, but the cloaca remains. These structures are ejected from the rupture of the anterior end of the body leading to a hole (wound) [33]. In posterior evisceration, which mainly occurs in the holothuriids and synallactids, the digestive tract between the oesophagus and the cloaca is lost, along with attached haemal vessels, one or two respiratory trees, and sometimes the gonads. These structures are ejected through the cloaca, although in a few species it occurs directly through the body wall (e.g., Stichopus chloronotus [39], Holothuria difficilis, H. surinamensis, and H. parvula) [19].
During the posterior evisceration process, coelomic fluid is ejected from the cloaca, followed by acute contractions of the body wall. The pharyngeal retractor expands, and the tentacles and tube feet swing rapidly and irregularly [40]. Species that undergo anterior evisceration exhibit rapid softening of the ligaments and tendons as well as rupture of the introvert [19,24,35]. After evisceration, the body wall slowly returns to the relaxed state.
The function of the collagenous tissues in the sea cucumber body is to switch between rigid and flexible states to maintain rapid changes in mechanical properties, but irreversible disintegration of the collagenous tissues occurs during autotomy [24,25,26]. At the fracture plane, the most abundant soft tissue with mechanical significance is mutable collagenous tissue (MCT). MCT exists in the form of dermal connective tissue, interossicular ligaments, and tendons, and it is involved in the autotomy mechanism in all echinoderms [41]. A small fraction of MCTs contain myocytes, and all MCTs contain cells rich in inhomogeneous vacuoles, which seems to be lysosomal [41]. Additionally, juxtaligamental cells, which contain electron-dense granules, are effector cells that directly change the tensile properties of MCT [41]. The detachment mechanism of the evisceration process depends on the rapid, irreversible loss of tensile strength of MCT at the autotomy plane [26]. Changes in MCT tensility do not involve changes in the mechanical properties of hollow microfibrils or collagen fibrils, and the mutability depends on changes in the cohesion that holds the fibrils together. Juxtaligamental granules in the dermis of sea cucumbers contain chemical factors that affect interfibrillar cohesion and are sensitive to changes in extracellular calcium ion concentration. Calcium ion concentration is the direct cause of MCT interfiber cohesion, and the stiffness of MCT becomes stronger when the concentration of Ca2+ increases [41]. Although juxtaligamental cells are not involved in intestinal autotomy, neurosecretory-like processes containing large dense vesicles scattered through the connective tissue control MCT mutability [24]. MCTs also are permeated by granule-containing processes belonging to neurosecretory-like perikarya, which may be in synaptic contact with motor neurons [25].
The release of the neurosecretory evisceration factor (EF) into the coelomic fluid results in a complete loss of tension in the connective tissue matrix [25,32], triggering autotomy, and muscle contraction facilitates the separation of the autotomy structures [24]. However, autotomy may occur during both the relaxation and the contraction phase of a cycle rather than at maximum tension [42]. Neither the radial nerve cord nor the nerve ring is an abundant source of EF; instead, it is found primarily in the axonal tracts of the haemal system [42]. Peritoneum is another major source of EF [24]. EF is a multifunctional molecule [42] that also exists in non-eviscerating species [32]. It may be a neuropeptide, but little is known about its chemical properties and whether it consists of one or several molecules [24]. Although the A. japonicus genome is available, it is still unclear what the EF is. Mechanical, chemical, and hypoxic stress can all lead to evisceration [43], which is a complex physiological process under the control of nerves. Before and after evisceration, genes related to muscle contraction and neurotransmitter secretion are differentially expressed [40], and metabolites related to oxidative stress and nerves change significantly [44].
In the laboratory, evisceration induction usually involves intraperitoneal injection of potassium chloride (KCl) solution at a concentration of 0.35 mol/L (lower than the osmolality of coelomic fluid (0.54 mol/L)) or occasionally distilled water [42]. The potassium ion is a nerve active substance, and injection of KCl solution into the coelom stimulates the release of EF and acetylcholine. The combined effect of the two leads to decreased MCT viscosity and muscle contraction, ultimately leading to tissue breakdown [32,41,42].
Many studies have explored the histological and physiological changes that occur during holothurian evisceration and regeneration [39,45,46,47], but few studies have focused on the molecular mechanism responsible for evisceration. Using Illumina sequencing, Ding et al. [40] investigated the genes that are differentially expressed during the evisceration process of A. japonicus and identified genes involved in muscle contraction, hormone and neurotransmitter secretion, nerve and muscle damage, energy support, cellular stress, and apoptosis.

3. Histology and Cytology of Intestinal Regeneration

Understanding the histological and cytological changes that occur during various stages of regeneration of the holothurian digestive tract requires knowledge of the structure of the normal digestive tract (Figure 1A). From the mouth to the cloaca, the digestive tract is roughly divided into the oral complex, pharynx, esophagus, stomach (although some species, such as H. glaberimma, do not have a stomach [48]), intestine, and cloaca. In some species, the size and structure of each region is determined by feeding behavior and digestive tract physiology, so it is species-specific.
Studies of the histology and cytology of the intestinal wall of sea cucumbers indicate slight differences in tissue layers and cellular composition among species or in different regions of the digestive tract [49,50,51], but the structural composition is similar. Tissues of the intestinal wall from outside to inside are as follows: coelomic epithelium exposed to the coelom, outer connective tissue, longitudinal muscles, circular muscles, inner connective tissue, and luminal epithelium, which is an endodermal derivative [52] that is exposed to the intestinal lumen [19,48,53]. The luminal epithelium is monolayer or pseudostratified columnar epithelium, mainly composed of closely arranged columnar epithelial cells, mucous cells, and digestive cells [19,51,53]. The loose inner connective tissue, which is mesodermally derived [52], contains collagen fibers, undifferentiated cells, lymphocytes, amoebocytes, and morula cells [51]. The muscle layer, which is also mesodermally derived [52], is arranged in two different directions. The phagocytic coelomocytes are localized in the basement membrane of the coelomic epithelium (also called peritonaeum), and their long, narrow, basal projections extend through the muscle layers and nerves to the connective tissue [53]. Coelomic epithelium is mainly composed of peritoneocytes and myoepithelial cells [51,54] with adjacent outer connective tissue. Some researchers refer to the coelomic epithelium, outer connective tissue, and muscle layer together as the mesothelium [55]. Recent studies suggest that there are three main tissue layers: the mesothelium, a connective tissue layer, and the luminal epithelium [23].
The digestive tract is anchored to the body wall by a continuous mesenteric network and suspended in the body cavity. According to the position of each part of the mesentery, it is referred to as dorsal, ventral, or lateral [19]. The structure of the mesentery is mesothelial layer–connective tissue layer–mesothelial layer, which means that the mesentery contains all of the histological components that make up the digestive tract, except for the luminal epithelium [19,56].

3.1. Wound Healing Stage

Ruptures of the esophagus, stomach, and cloaca heal into a blind end, and the edge of the mesentery is repaired rapidly to prepare for the formation of a new intestine [27]. In the anterior evisceration holothurians, the wound at the front of the body is closed due to the contraction of the body wall, where radial longitudinal muscles and water canals are converged [33,34]. During this process, a disordered muscle layer can be observed, and the myofilaments condense into a compact spindle-like structure (SLS), which is a sign of muscle dedifferentiation [27,57,58,59,60,61]. At this stage, the number of juvenile cells thought to be the stem cells of coelomocytes increases and differentiates into amoebocytes and morula cells, and the amoebocytes perform phagocytic functions [34,57,61,62]. Morula cells are involved in the cutaneous wound healing process, but not in intestinal regeneration, as the number decreases at the initial stage of intestinal regeneration [63,64]. SLSs are accumulated in the cytoplasm and subsequently expelled outside the cell to be phagocytosed by amoebocytes [65], thus, cells with SLSs present in the cytoplasm are not necessarily dedifferentiated muscle cells. Peritoneal cells, the other major cell type of the mesothelium, also dedifferentiate at this stage, with fascicular intermediate filaments splitting into short fragments [34].
Wounds are responsible for the stress condition, which induces cellular and biochemical responses. Vazzana et al. [66] reported increased expression of heat shock protein 70, which is expressed in coelomocytes, and speculated that the function is to induce cell differentiation and migration to the fracture plane.

3.2. Anlage Formation Stage

Intestinal regeneration begins at the free end of the mesentery [19,48,55,56,57]. Initially, differentiated cells in the mesothelial layers dedifferentiate and transdifferentiate in preparation for migration to the free end of the mesentery, where regeneration starts [54,56,67]. There is no direct evidence that stem cells in the traditional sense are involved in regeneration [54,68]. Apart from the thickening of the mesenteric margin, two anlages [34] form at the esophagus and cloaca in posterior eviscerating species such as A. japonicus [69] and H. glaberrima [48], whereas the anterior anlage consists of a mass of cells and posterior anlage, namely a blind tube, in anterior eviscerating species such as Eupentacta fraudatrix [34] and E. quinquesemita [33].
The content of the extracellular matrix changes significantly during anlage formation. The content of fibrocollagen and collagen decreases in a gradient from the free end of the mesentery to the body wall, which is related to the proteolytic activity of matrix metalloproteinases [64,70], and the same pattern occurs for myocytes [23]. At this stage, a great deal of myocytes dedifferentiation and migration occurs. During migration, the cells lose their dedifferentiation characteristics, and tonofilaments and spindle-like structures disappear from the cytoplasm [34,54]. Dedifferentiation of myocytes might result in cell proliferation [57], but only a small amount of cell proliferation occurs at this stage; the level increases in the next stage [48]. This has been demonstrated in several species, including H. glaberrima, Stichopus mollis, and E. fraudatrix [64]. García-Arrarás et al. [67] reported that some epithelial cells sank into connective tissue and became mesenchymal cells at 3 days post-evisceration in H. glaberrima. In another study, Okada and Kondo [33] found mesenchymal cells in the anterior mesentery margin and during the mesenchymal–epithelial transition (MET) in E. quinquesemita, but there is no MET in E. fraudatrix because this species retains intercellular junctions [34,71]. In the next step, dedifferentiated cells proliferate, thereby providing cells for the intestinal rudiment. Apoptosis, which is activated by injury or remodeling, is a cytological event accompanied by cell proliferation and may be an inducer of cell proliferation [23,72].

3.3. Lumen Formation Stage

The intestinal lumen is formed in opposite directions from the anterior and posterior ends and completely connects into a thin and fragile intestinal tube. Regardless of the anterior evisceration or the posterior evisceration, the luminal epithelium of the posterior anlage originates from the endoderm-derived lining epithelium of the cloacal [34,48]. In the anterior anlage, there are two variants. For the posterior evisceration sea cucumbers, represented by H. glaberrima and A. japonicus, the esophagus remains in the coelom after evisceration [27,37,48]. The new intestinal lumen is formed by the invasion of tubular residues of the esophagus into the amorphous matrix of the thickened connective tissue [27]. For the anterior evisceration sea cucumbers, represented by E. quinquesemita and E. fraudatrix, the entire gut and oral complex are excluded except the cloaca [33,34]. The anterior mesenteric margin thickens to form the anlage filled with mesenchymal cells. Mesenchymal cells differentiate into epithelial-like cells, and multiple cavities are formed [33].
The lumen epithelium is derived from the proliferation and differentiation of cells in the esophageal mesothelium, and the proliferation of epithelial cells forms the intestinal lumen [40,48,73]. During this stage, cell division is most active in the luminal epithelium and is evenly distributed [27], with a gradual increase in the coelomic epithelium and muscular layers.

3.4. Intestinal Differentiation Stage

This stage is dominated by the proportioning of tissue layers and differentiation of different regions of the intestine. The intestinal tract develops digestive function and forms a typical S-shaped structure. Once the intestinal cavity is formed, the new intestine develops from a rudiment with only two tissue layers into a new intestine with three tissue layers [23,74]. The differentiated cells in the luminal epithelium undergo cell differentiation and proliferation so that the intestine gradually becomes a multicellular layer of tissue.

3.5. Intestinal Growth Stage

After intestinal differentiation, the digestive tract is structurally and cellularly indistinguishable from the normal intestine, but it is smaller in size than the normal intestine. The tissue layers of the intestinal wall thicken further, eventually returning to the size of the intestine before evisceration. It takes about 1 month for the regenerative intestines of A. japonicus and H. glaberrima to return to the same morphology and function as normal intestines.

4. Molecular Mechanism of Intestinal Regeneration

4.1. Intestinal Regeneration

Studies of the molecular mechanisms involved in intestinal regeneration in sea cucumbers began around 2000 [27], so little is known and much remains unknown. Prior to the development of sequencing technology, studies of the molecular mechanisms of sea cucumber regeneration focused on functional exploration of individual genes (i.e., regeneration-related or developmentally relevant genes that have been studied in other animals) [27]. In situ hybridization or Northern blot and immunohistochemistry are used to localize, quantify, and characterize the transcripts and translated proteins of candidate genes to determine when and where the genes are expressed. Santiago et al. [75] cloned the serum amyloid A (SAA) protein gene from the cDNA library of the regenerating intestine of H. glaberrima [76] and found that its expression increased during the regeneration process, reaching a peak at 15 days post-evisceration. In another study, Santiago-Cardona et al. [77] reported that lipopolysaccharides could induce an increase in SAA mRNA levels in non-regenerating intestines. Suárez-Castillo et al. [78] and Zheng et al. [79] cloned the Ependymin gene from regenerating libraries of H. glaberrima and A. japonicus, respectively, and found that it was overexpressed during intestinal regeneration. Mashanov et al. [72] reported that two pro-cancer genes, survivin and mortalin, were overexpressed only during regeneration in the sea cucumber, and Mashanov et al. [80] found that Wnt9, TCTP, and Bmp1/Tll, which have been previously known to be implicated in embryogenesis and cancer, were up-regulated in visceral regeneration in the sea cucumber H. glaberrima. In addition to cloning genes from regenerating libraries, researchers also obtained genes by rapid amplification of cDNA ends polymerase chain reaction, such as Wnt6 and Hox6 [81], elongator protein 2 (Ajelp2) [82], WntA [83], and Krüppel-like factor13 (klf13) [74].
Adams et al. [84] first proposed the concept of expressed sequence tags (ESTs), which is a classical technique complementary to other methodologies such as nucleic acid microarray and serial analysis of gene expression. Zheng et al. [79] and Rojas-Cartagena et al. [85] successively constructed the cDNA libraries of the early regenerating intestine and the normal intestine of A. japonicus and H. glaberrima, and they analyzed the ESTs from these two libraries for each species. For the first time, the functions of differentially expressed genes were outlined, with decreased expression of genes involved in metabolism and increased expression of genes involved in immunity, division, and cell signaling transduction in the regenerating intestine [79]. Subsequently, Ortiz-Pineda et al. [52] constructed the gene expression profile based on the EST database for the anlage and lumen formation stages. With the development of high-throughput sequencing technology, Sun et al. [86] constructed a library containing 182,473 reads using 454 cDNA sequencing technology. Transcriptome sequencing technology overcomes the shortcomings of microarray technology, such as low throughput, high background signal interference, and low sensitivity, and it is an effective tool for constructing high-quality expression profiles. Sun et al. [87] constructed gene expression profiles of A. japonicus in different regeneration stages and used RNA-seq technology to reveal the dynamic changes in gene expression that occur during regeneration. The gene expression profiles mentioned above were based on transcriptome data without a reference genome because the first whole genome sequence of the sea cucumber was not constructed until 2017 [88]. Through scaffold splicing and amino acid sequences analysis, Zhang et al. [88] found two gene families: Prostatic secretory protein of 94 amino acids (PSP94)-like genes, which have 11 tandem duplications, were overexpressed during the wound healing stage; and fibrinogen-related protein genes, which have 21 tandem duplications, were significantly up-regulated during the early and middle stages of visceral regeneration. The whole genome of H. glaberrima has also been constructed and the melanotransferrin (Mtf) gene family was found to be significantly expanded in this sea cucumber [89]. Fumagalli et al. [2] mapped transcriptomic data from different stages of the hydra Hydra magnipapillata, the flatworm Schmidtea mediterranea, and A. japonicus to the same reference database and screened for some regenerative conserved genes. These three species share 18 regenerative differentially expressed genes with humans, and their functions involve “proteases involved in developmental processes”, “calcium ion binding”, “cell junction”, “structure and migration”, and “cancer-related scramblase ANO7”.
Intestinal regeneration involves the coordinated regulation of multiple genes, so in addition to focusing on the function of individual genes, researchers have explored related signaling pathways and gene families. The Wnt signaling pathway plays a role in the differential expression of genes during intestinal regeneration via three upstream genes (Wnt7, Fz7, Dvl) and one downstream gene (c-Myc), which are conserved and highly expressed [90]. The retinoic acid signaling pathway can regulate cell division and dedifferentiation [91]. The main gene families involved in regeneration are Wnt, Frizzled [68,92], Hox [76,88], and Mtf [89]. Wnt/β-catenin is a canonical signaling pathway associated with development and regeneration and can promote regeneration in axolotl, Xenopus, and zebrafish [93]. Hox genes are involved in creating positional information in polychaetes and planarians [94]. We have selected some genes, which are studied for their expression patterns by immunohistochemistry and listed in Table 2, which suggested that intestinal regeneration is a multi-gene-regulated process.
In addition to the spatiotemporal expression of genes, researchers have also focused on the function of gene products. Matrix metalloproteinase inhibitors cause disruption [70] or delay [99] of intestinal regeneration processes. Viera-Vera and García-Arrarás [91] reported that an inhibitor (citral) of the enzyme synthesizing retinoic acid reduced the size of the regenerated intestine. Additionally, a Wnt pathway inhibitor (iCRT14) was found to block cell dedifferentiation and reduce the size of the regenerated intestine, whereas a Wnt pathway activator (LiCl) increased its size [100]. RNA interference, which identifies specific gene sequences and their functional roles, is also being applied to echinoderms. Yuan et al. [90] reported that down-regulation of Wnt7 and Dvl expression by RNA interference significantly inhibited intestinal elongation. An in vitro primary cell culture system for sea cucumbers has been established [101], and it provides support for modern molecular biology techniques such as gene transfer and single cell transcriptomics.

4.2. Neural Components Regeneration

The enteric nervous system is arranged in plexuses and is distributed in all tissue layers of the mesentery and intestine. These plexuses are interconnected networks of neurons, axons, and enteric glial cells [102]. The mesenteric mesothelium has extensive fiber tracts and associated neurons that extend from the body wall to the intestine. Neuron-like cells and fibers are also present in the connective tissue, but the abundance and presence are different from the mesothelial layer with small amounts of nerve fibers that separate from each other. Nerve fibers and cells in mesentery rarely undergo morphological changes during regeneration, with only mild disturbance of the mesothelial plexus and degradation of fibers from the free end of the mesentery [55]. Neurodegenerative processes are also observed in the anlage, and the degradation is not completed until 10 dpe. Onset of innervation is observed in the mesothelial layer only after intestinal lumen formation (14 dpe). After that, nerve fibers in the connective tissue projected from mesothelial layer are observed. After the formation of luminal epithelium, neurons and neuroendocrine cells appear [103]. Consequently, regeneration of neural components lags behind intestinal regeneration.

5. Conclusions

Sea cucumber intestinal regeneration has been studied for many years. In this article, we reviewed the process of autotomy, evisceration, and regeneration of sea cucumbers, and described the cytological changes. With the development of high-throughput sequencing technology, the genomes of multiple sea cucumber species have been assembled, which greatly promoted the progress of molecular research on intestinal regeneration. However, there are still many unsolved mysteries. What substance causes the sea cucumber to eviscerate, neuropeptide or hormone? Is the regenerative anlage formed just by cell dedifferentiation and transdifferentiation, and are there stem cells in the body? By what mechanism does the sea cucumber initiate regeneration?
From an evolutionary perspective, compared to other regeneration model species, such as planarians, zebrafish, and amphibians, echinoderms are still left behind. Single-cell transcriptomes have been applied to the regeneration of hydra [104], planaria [105], amphibian [106], and zebrafish [107], and cell types important for regeneration have been discovered. In the future, single-cell transcriptome and spatial transcriptome will help reveal typical cell type and spatial orientation. Thus, we can analyze the conserved molecular mechanism regulating regeneration process among achordate and chordate.

Author Contributions

F.S., L.S. and H.Y. jointly designed the structure, wrote, and edited this article. All authors contributed to manuscript writing and approved the final version for submission. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China (grant numbers No. 42076093).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

All authors declare that they have no conflict of interest.

References

  1. Alvarado, A.S. Regeneration in the metazoans: Why does it happen? BioEssays 2000, 22, 579. [Google Scholar] [CrossRef]
  2. Fumagalli, M.R.; Zapperi, S.; La Porta, C.A.M. Regeneration in distantly related species: Common strategies and pathways. NPJ Syst. Biol. Appl. 2018, 4, 5. [Google Scholar] [CrossRef] [Green Version]
  3. Zeng, A.; Li, H.; Guo, L.; Gao, X.; McKinney, S.; Wang, Y.; Yu, Z.; Park, J.; Semerad, C.; Ross, E.; et al. Prospectively Isolated Tetraspanin (+) Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration. Cell 2018, 173, 1593–1608.e20. [Google Scholar] [CrossRef] [Green Version]
  4. Boehm, A.-M.; Khalturin, K.; Erxleben, F.A.; Hemmrich, G.; Klostermeier, U.C.; Lopez-Quintero, J.A.; Oberg, H.-H.; Puchert, M.; Rosenstiel, P.; Wittlieb, J.; et al. FoxO is a critical regulator of stem cell maintenance in immortal Hydra. Proc. Natl. Acad. Sci. USA 2012, 109, 19697–19702. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Galliot, B.; Ghila, L. Cell plasticity in homeostasis and regeneration. Mol. Reprod. Dev. 2010, 77, 837–855. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  6. Nakatani, Y.; Kawakami, A.; Kudo, A. Cellular and molecular processes of regeneration, with special emphasis on fish fins. Dev. Growth Differ. 2007, 49, 145–154. [Google Scholar] [CrossRef] [PubMed]
  7. Arenas Gomez, C.M.; Gomez Molina, A.; Zapata, J.D.; Delgado, J.P. Limb regeneration in a direct-developing terrestrial salamander, Bolitoglossa ramosi (Caudata: Plethodontidae): Limb regeneration in plethodontid salamanders. Regeneration 2017, 4, 227–235. [Google Scholar] [CrossRef]
  8. Bhavsar, R.B.; Nakamura, K.; Tsonis, P.A. A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt. J. Vis. Exp. 2011, 52, e2713. [Google Scholar] [CrossRef]
  9. Brockes, J.P. Amphibian limb regeneration: Rebuilding a complex structure. Science 1997, 276, 81–87. [Google Scholar] [CrossRef]
  10. Dunoyer, L.A.; Seifert, A.W.; Van Cleve, J. Evolutionary bedfellows: Reconstructing the ancestral state of autotomy and regeneration. J. Exp. Zool. Part B Mol. Dev. Evol. 2020, 336, 94–115. [Google Scholar] [CrossRef]
  11. Stocum, D.L. Vertebrate regeneration. Semin. Cell Dev. Biol. 2002, 13, 325–326. [Google Scholar] [CrossRef] [PubMed]
  12. Carnevali, M.D.C. Regeneration in Echinoderms: Repair, regrowth, cloning. Inf. Syst. J. 2006, 3, 64–76. [Google Scholar]
  13. Hernroth, B.; Farahani, F.; Brunborg, G.; Dupont, S.; Dejmek, A.; Skold, H.N. Possibility of mixed progenitor cells in sea star arm regeneration. J. Exp. Zool. B Mol. Dev. Evol. 2010, 314, 457–468. [Google Scholar] [CrossRef]
  14. Huet, M. Ultrastructural study and development of the neuroepithelial cells of regenerating epidermic arm cells in Asterina gibbosa Penn. Z. Zellforsch. Mikrosk. Anat. 1972, 126, 75–89. [Google Scholar] [CrossRef] [PubMed]
  15. Huet, M. Role of the nervous system during the regeneration of an arm in a starfish: Asterina gibbosa Penn. (Echinodermata, Asteriidae). J. Embryol. Exp. Morphol. 1975, 33, 535–552. [Google Scholar] [PubMed]
  16. Vickery, M.S.; McClintock, J.B. Regeneration in metazoan larvae. Nature 1998, 394, 140. [Google Scholar] [CrossRef]
  17. Makra, A.; Keegan, B.F. Arm regeneration in Acrocnida brachiata (Ophiuroidea) at Little Killary, west coast of Ireland. Biol. Environ. Proc. R. Ir. Acad. 1999, 99B, 95–102. [Google Scholar]
  18. Reinardy, H.C.; Emerson, C.E.; Manley, J.M.; Bodnar, A.G. Tissue regeneration and biomineralization in sea urchins: Role of Notch signaling and presence of stem cell markers. PLoS ONE 2015, 10, e0133860. [Google Scholar] [CrossRef] [Green Version]
  19. García-Arrarás, J.E.; Greenberg, M.J. Visceral regeneration in holothurians. Microsc. Res. Tech. 2001, 55, 438–451. [Google Scholar] [CrossRef]
  20. Bertolini, F. Rigenerazione dell’Apparato Digerente nello Stichopus Regalis. Publ. Stn. Zool. Napoli. 1930, 10, 439–448. [Google Scholar]
  21. Zang, Y.Q.; Tian, X.L.; Dong, S.L.; Dong, Y.W. Growth, metabolism and immune responses to evisceration and the regeneration of viscera in sea cucumber, Apostichopus japonicus. Aquaculture 2012, 358-359, 50–60. [Google Scholar] [CrossRef]
  22. Bai, M.M. Regeneration in holothurian, holothuria-scabra jager. Indian J. Exp. Biol. 1971, 9, 467. [Google Scholar] [PubMed]
  23. García-Arrarás, J.E.; Lázaro-Peña, M.I.; Díaz-Balzac, C.A. Holothurians as a Model System to Study Regeneration. In Marine Organisms as Model Systems in Biology and Medicine; Kloc, M., Kubiak, J.Z., Eds.; Springer International Publishing: Cham, Switzerland, 2018; pp. 255–283. [Google Scholar]
  24. Byrne, M. The morphology of autotomy structures in the sea cucumber Eupentacta quinquesemita before and during evisceration. J. Exp. Biol. 2001, 204, 849–863. [Google Scholar] [CrossRef] [PubMed]
  25. Wilkie, I.C. Variable tensility in echinoderm collagenous tissues—A review. Mar. Behav. Physiol. 1984, 11, 1–34. [Google Scholar] [CrossRef]
  26. Wilkie, I.C. Autotomy as a prelude to regeneration in echinoderms. Microsc. Res. Tech. 2001, 55, 369–396. [Google Scholar] [CrossRef]
  27. Mashanov, V.; García-Arrarás, J. Gut regeneration in holothurians: A snapshot of recent developments. Biol. Bull. 2011, 221, 93–109. [Google Scholar] [CrossRef]
  28. Miller, A.K.; Kerr, A.M.; Paulay, G.; Reich, M.; Wilson, N.G.; Carvajal, J.I.; Rouse, G.W. Molecular phylogeny of extant Holothuroidea (Echinodermata). Mol. Phylogenet. Evol. 2017, 111, 110–131. [Google Scholar] [CrossRef] [Green Version]
  29. Pearse, A.S. Autotomy in holothurians. Biol. Bull. 1909, 18, 42–49. [Google Scholar] [CrossRef]
  30. Byrne, M. The case for seasonal evisceration in the holothyroid Eupentacta quinquesemita (selenka), a reply. Can. J. Zool. 1986, 64, 2391–2392. [Google Scholar] [CrossRef]
  31. Swan, E.F. Seasonal evisceration in the sea cucumber, Parastichopus californicus (Stimpson). Science 1961, 133, 1078–1079. [Google Scholar] [CrossRef]
  32. Byrne, M. Induction of Evisceration in the Holothurian Eupentacta quinquesemita and Evidence for the Existence of an Endogenous Evisceration Factor. J. Exp. Biol. 1986, 120, 25–39. [Google Scholar] [CrossRef]
  33. Okada, A.; Kondo, M. Regeneration of the digestive tract of an anterior-eviscerating sea cucumber, Eupentacta quinquesemita, and the involvement of mesenchymal-epithelial transition in digestive tube formation. Zool. Lett. 2019, 5, 21. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  34. Mashanov, V.S.; Dolmatov, I.Y.; Heinzeller, T. Transdifferentiation in holothurian gut regeneration. Biol. Bull. 2005, 209, 184–193. [Google Scholar] [CrossRef] [PubMed]
  35. Kille, F.R. Regeneration in Thyone briareus Lesueur following induced autotomy. Biol. Bull. 1935, 69, 82–108. [Google Scholar] [CrossRef]
  36. Weigel, B.L. Sea Cucumber Intestinal Regeneration Reveals Deterministic Assembly of the Gut Microbiome. Appl. Environ. Microbiol. 2020, 86, e00489-20. [Google Scholar] [CrossRef] [PubMed]
  37. Shukalyuk, A.; Dolmatov, I.Y. Regeneration of the Digestive Tube in the Holothurian Apostichopus japonicusafter Evisceration. Russ. J. Mar. Biol. 2001, 27, 168–173. [Google Scholar] [CrossRef]
  38. Moussa Moussa, R.; El-Saied Nasef, L.; Abdel-Latif, H.A.A. Evisceration induction and gut regeneration of Holothuria polii (Delle Chiaje, 1824). Cah. Biol. Mar. 2018, 59, 309–315. [Google Scholar]
  39. Dolmatov, I.Y.; Khang, N.A.; Kamenev, Y.O. Asexual reproduction, evisceration, and regeneration in holothurians (Holothuroidea) from Nha Trang Bay of the South China Sea. Russ. J. Mar. Biol. 2012, 38, 243–252. [Google Scholar] [CrossRef]
  40. Ding, K.; Zhang, L.; Sun, L.; Lin, C.; Feng, Q.; Zhang, S.; Yang, H.; Brinkman, R.; Lin, G.; Huang, Z. Transcriptome analysis provides insights into the molecular mechanisms responsible for evisceration behavior in the sea cucumber Apostichopus japonicus. Comp. Biochem. Physiol. Part D Genom. Proteom. 2019, 30, 143–157. [Google Scholar] [CrossRef]
  41. Wilkie, I.C. Mutable collagenous tissue: Overview and biotechnological perspective. Prog. Mol. Subcell. Biol. 2005, 39, 221–250. [Google Scholar]
  42. Smith, G.N.; Greenberg, M.J. Chemical control of evisceration process in Thyone briareus. Biol. Bull. 1973, 144, 421–436. [Google Scholar] [CrossRef]
  43. Jenzri, M.; Gharred, C.; Bouraoui, Z.; Guerbej, H.; Jebali, J.; Gharred, T. Evisceration of Holothuria poli by mechanical, chemical and hypoxia stress methods and its bioremediation potentials for the pisciculture wastewater. Aquac. Res. 2022, 53, 3309–3317. [Google Scholar] [CrossRef]
  44. Ding, K.; Zhang, L.; Huo, D.; Guo, X.; Liu, X.; Zhang, S. Metabolomic analysis of coelomic fluids reveals the physiological mechanisms underlying evisceration behavior in the sea cucumber Apostichopus japonicus. Aquaculture 2021, 543, 736960. [Google Scholar] [CrossRef]
  45. Dobson, W.E.; Turner, R.L. Morphology and histology of the disk autotomy plane in Ophiophragmus filograneus (Echinodermata, Ophiurida). Zoomorphology 1989, 108, 323–332. [Google Scholar] [CrossRef]
  46. Dolmatov, I.Y. Asexual reproduction in holothurians. Sci. World J. 2014, 2014, 527234. [Google Scholar] [CrossRef] [Green Version]
  47. Dolmatov, I.Y. New data on asexual reproduction, autotomy, and regeneration in holothurians of the Order Dendrochirotida. Russ. J. Mar. Biol. 2014, 40, 228–232. [Google Scholar] [CrossRef]
  48. García-Arrarás, J.; Estrada-Rodgers, L.; Santiago, R.; Torres, I.I.; Díaz-Miranda, L.; Torres-Avillán, I. Cellular mechanisms of intestine regeneration in the sea cucumber, Holothuria glaberrima Selenka (Holothuroidea: Echinodermata). J. Exp. Zool. 1998, 281, 288–304. [Google Scholar] [CrossRef]
  49. Féral, J.-P.; Massin, C. Digestive systems: Holothurioidea. In Echinoderm Nutrition; Balkema: Rotterdam, The Netherlands, 1982; pp. 191–212. [Google Scholar]
  50. Foster, G.G.; Hodgson, A.N. Feeding, tentacle and gut morphology in five species of southern African intertidal holothuroids (Echinodermata). S. Afr. J. Zool. 1996, 31, 70–79. [Google Scholar] [CrossRef]
  51. Mashanov, V.; Frolova, L.; Dolmatov, I.Y. Structure of the digestive tube in the holothurian Eupentacta fraudatrix (Holothuroidea: Dendrochirota). Russ. J. Mar. Biol. 2004, 30, 314–322. [Google Scholar] [CrossRef]
  52. Ortiz-Pineda, P.A.; Ramírez-Gómez, F.; Pérez-Ortiz, J.; González-Díaz, S.; Santiago-De Jesús, F.; Hernández-Pasos, J.; Del Valle-Avila, C.; Rojas-Cartagena, C.; Suárez-Castillo, E.C.; Tossas, K.; et al. Gene expression profiling of intestinal regeneration in the sea cucumber. BMC Genom. 2009, 10, 262. [Google Scholar] [CrossRef] [Green Version]
  53. Filimonova, G.F.; Tokin, I.B. Structural and functional peculiarities of the digestive-system of Cucumaria frondosa (Echinodermata, Holothuroidea). Mar. Biol. 1980, 60, 9–16. [Google Scholar] [CrossRef]
  54. Boyko, A.V.; Girich, A.S.; Tkacheva, E.S.; Dolmatov, I.Y. The Eupentacta fraudatrix transcriptome provides insights into regulation of cell transdifferentiation. Sci. Rep. 2020, 10, 1522. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  55. Nieves-Rios, C.; Alvarez-Falcon, S.; Malavez, S.; Rodriguez-Otero, J.; Garcia-Arraras, J.E. The nervous system component of the mesentery of the sea cucumber Holothuria glaberrima in normal and regenerating animals. Cell Tissue Res. 2019, 380, 67–77. [Google Scholar] [CrossRef] [PubMed]
  56. Garcia-Arraras, J.E.; Bello, S.A.; Malavez, S. The mesentery as the epicenter for intestinal regeneration. Semin. Cell Dev. Biol. 2019, 92, 45–54. [Google Scholar] [CrossRef]
  57. Candelaria, A.G.; Murray, G.; File, S.K.; García-Arrarás, J.E. Contribution of mesenterial muscle dedifferentiation to intestine regeneration in the sea cucumber Holothuria glaberrima. Cell Tissue Res. 2006, 325, 55–65. [Google Scholar] [CrossRef]
  58. Carnevali, M.D.C.; Bonasoro, F. Microscopic overview of crinoid regeneration. Microsc. Res. Tech. 2001, 55, 403–426. [Google Scholar] [CrossRef]
  59. Dolmatov, I.Y. Regeneration of the aquapharyngeal complex in the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota). Monogr. Dev. Biol. 1992, 23, 40. [Google Scholar]
  60. Dolmatov, I.Y.; Eliseikina, M.G.; Ginanova, T.; Lamash, N.; Korchagin, V.; Bulgakov, A. Muscle regeneration in the holothurian Stichopus japonicus. Dev. Genes Evol. 1996, 205, 486–493. [Google Scholar] [CrossRef]
  61. Dolmatov, I.Y.; Ginanova, T.T. Muscle regeneration in holothurians. Microsc. Res. Tech. 2001, 55, 452–463. [Google Scholar] [CrossRef]
  62. Dolmatov, I.Y. Molecular Aspects of Regeneration Mechanisms in Holothurians. Genes 2021, 12, 250. [Google Scholar] [CrossRef]
  63. Menton, D.N.; Eisen, A.Z. Cutaneous wound-healing in sea cucumber, Thyone-Briareus. J. Morphol. 1973, 141, 185–203. [Google Scholar] [CrossRef] [PubMed]
  64. San Miguel-Ruiz, J.E.; Garcia-Arraras, J.E. Common cellular events occur during wound healing and organ regeneration in the sea cucumber Holothuria glaberrima. BMC Dev. Biol. 2007, 7, 115. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  65. Jose, E. Garcia-Arraras, I.Y.D. Echinoderms: Potential Model Systems for Studies on Muscle Regeneration. Curr. Pharm. Des. 2010, 16, 942–955. [Google Scholar]
  66. Vazzana, M.; Siragusa, T.; Arizza, V.; Buscaino, G.; Celi, M. Cellular responses and HSP70 expression during wound healing in Holothuria tubulosa (Gmelin, 1788). Fish Shellfish Immunol. 2015, 42, 306–315. [Google Scholar] [CrossRef]
  67. García-Arrarás, J.E.; Valentin-Tirado, G.; Flores, J.E.; Rosa, R.J.; Rivera-Cruz, A.; San Miguel-Ruiz, J.E.; Tossas, K. Cell dedifferentiation and epithelial to mesenchymal transitions during intestinal regeneration in H. glaberrima. BMC Dev. Biol. 2011, 11, 61–78. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  68. Girich, A.S.; Boyko, A.V. Wnt and Frizzled Genes in Echinoderms. Russ. J. Mar. Biol. 2019, 45, 302–312. [Google Scholar] [CrossRef]
  69. Leibson, N. Regeneration of digestive tube in holothurians Stichopus japonicus and Eupentacta fraudatrix. Monogr. Dev. Biol. 1992, 23, 51–61. [Google Scholar] [PubMed]
  70. Quinones, J.L.; Rosa, R.; Ruiz, D.L.; Garcia-Arraras, J.E. Extracellular matrix remodeling and metalloproteinase involvement during intestine regeneration in the sea cucumber Holothuria glaberrima. Dev. Biol. 2002, 250, 181–197. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  71. Mashanov, V.S.; Dolmatov, I.Y. Regeneration of digestive tract in the pentactulae of the far-eastern holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota). Invertebr. Reprod. Dev. 2001, 39, 143–151. [Google Scholar] [CrossRef]
  72. Mashanov, V.S.; Zueva, O.R.; Rojas-Catagena, C.; García-Arrarás, J.E. Visceral regeneration in a sea cucumber involves extensive expression of survivin and mortalin homologs in the mesothelium. BMC Dev. Biol. 2010, 10, 117–141. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  73. Dawbin, W.H. Auto-evisceration and the regeneration of viscera in the holothurian Stichopus mollis (Hutton). Trans. Proc. R. Soc. N. Z. 1949, 77, 497–523. [Google Scholar]
  74. Chen, L.; Yao, F.; Qin, Y.; Shao, Y.; Fang, L.; Yu, X.; Wang, S.; Hou, L. The potential role of Kruppel-like factor 13 (Aj-klf13) in the intestine regeneration of sea cucumber Apostichopus japonicus. Gene 2020, 735, 144407. [Google Scholar] [CrossRef] [PubMed]
  75. Santiago, P.; Roig-López, J.L.; Santiago, C.; García-Arrarás, J.E. Serum amyloid A protein in an echinoderm: Its primary structure and expression during intestinal regeneration in the sea cucumber Holothuria glaberrima. J. Exp. Zool. 2000, 288, 335–344. [Google Scholar] [CrossRef]
  76. Mendez, A.T.; Roig-Lopez, J.L.; Santiago, P.; Santiago, C.; Garcia-Arraras, J.E. Identification of Hox gene sequences in the sea cucumber Holothuria glaberrima Selenka (Holothuroidea: Echinodermata). Mar. Biotechnol. 2000, 2, 231–240. [Google Scholar] [CrossRef] [PubMed]
  77. Santiago-Cardona, P.G.; Berrios, C.A.; Ramirez, F.; Garcia-Arraras, J.E. Lipopolysaccharides induce intestinal serum amyloid A expression in the sea cucumber Holothuria glaberrima. Dev. Comp. Immunol. 2003, 27, 105–110. [Google Scholar] [CrossRef]
  78. Suarez-Castillo, E.C.; Medina-Ortíz, W.E.; Roig-López, J.L.; E García-Arrarás, J. Ependymin, a gene involved in regeneration and neuroplasticity in vertebrates, is overexpressed during regeneration in the echinoderm Holothuria glaberrima. Gene 2004, 334, 133–143. [Google Scholar] [CrossRef] [PubMed]
  79. Zheng, F.-X.; Sun, X.-Q.; Fang, B.-H.; Hong, X.-G.; Zhang, J.-X. Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida: Stichopodidae) and cloning of ependymin gene. Hydrobiologia 2006, 571, 109–122. [Google Scholar] [CrossRef]
  80. Mashanov, V.S.; Zueva, O.R.; Garcia-Arraras, J.E. Expression of Wnt9, TCTP, and Bmp1/Tll in sea cucumber visceral regeneration. Gene Expr. Patterns 2012, 12, 24–35. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  81. Sun, L.N.; Yang, H.S.; Chen, M.Y.; Xu, D.X. Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus. Genet. Mol. Res. 2013, 12, 5321–5334. [Google Scholar] [CrossRef]
  82. Mei, Y.L.; Yao, F.; Wu, Y.; Chu, B.; Cheng, C.; Liu, Y.; Li, X.J.; Zou, X.Y.; Hou, L. Identification and expression of the elongator protein 2 (Ajelp2) gene, a novel regeneration-related gene from the sea cucumber Apostichopus japonicus. Mol. Biol. Rep. 2014, 41, 4985–4996. [Google Scholar] [CrossRef] [PubMed]
  83. Li, X.N.; Sun, L.N.; Yang, H.S.; Zhang, L.B.; Miao, T.; Xing, L.L.; Huo, D. Identification and expression characterization of WntA during intestinal regeneration in the sea cucumber Apostichopus japonicus. Comp. Biochem. Phys. B Biochem. Mil. Biol. 2017, 210, 55–63. [Google Scholar] [CrossRef]
  84. Adams, M.D.; Kelley, J.M.; Gocayne, J.D.; Dubnick, M.; Polymeropoulos, M.H.; Xiao, H.; Merril, C.R.; Wu, A.; Olde, B.; Moreno, R.F.; et al. Complementary DNA Sequencing—Expressed Sequence Tags and Human Genome Project. Science 1991, 252, 1651–1656. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  85. Rojas-Cartagena, C.; Ortaz-Pineda, P.; Ramirez-Gomez, F.; Suarez-Castillo, E.C.; Matos-Cruz, V.; Rodriguez, C.; Ortiz-Zuazaga, H.; Garcia-Arraras, J.E. Distinct profiles of expressed sequence tags during intestinal regeneration in the sea cucumber Holothuria glaberrima. Physiol. Genom. 2007, 31, 203–215. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  86. Sun, L.; Chen, M.; Yang, H.; Wang, T.; Liu, B.; Shu, C.; Gardiner, D.M. Large Scale Gene Expression Profiling during Intestine and Body Wall Regeneration in the Sea Cucumber Apostichopus japonicus. Comp. Biochem. Physiol. Part. D Genom. Proteom. 2011, 6, 195–205. [Google Scholar] [CrossRef] [PubMed]
  87. Sun, L.; Yang, H.; Chen, M.; Ma, D.; Lin, C. RNA-Seq Reveals Dynamic Changes of Gene Expression in Key Stages of Intestine Regeneration in the Sea Cucumber Apostichopus japonicus. PLoS ONE 2013, 8, e69441. [Google Scholar]
  88. Zhang, X.J.; Sun, L.N.; Yuan, J.B.; Sun, Y.M.; Gao, Y.; Zhang, L.B.; Li, S.H.; Dai, H.; Hamel, J.F.; Liu, C.Z.; et al. The sea cucumber genome provides insights into morphological evolution and visceral regeneration. PLoS Biol. 2017, 15, e2003790. [Google Scholar] [CrossRef] [Green Version]
  89. Medina-Feliciano, J.G.; Pirro, S.; Garcia-Arraras, J.E.; Mashanov, V.; Ryan, J.F. Draft Genome of the Sea Cucumber Holothuria glaberrima, a Model for the Study of Regeneration. Front. Mar. Sci. 2021, 8, 603410. [Google Scholar] [CrossRef]
  90. Yuan, J.; Gao, Y.; Sun, L.; Jin, S.; Zhang, X.; Liu, C.; Li, F.; Xiang, J. Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus. Front. Genet. 2019, 10, 112. [Google Scholar] [CrossRef]
  91. Viera-Vera, J.; García-Arrarás, J.E. Retinoic Acid Signaling Is Associated with Cell Proliferation, Muscle Cell Dedifferentiation, and Overall Rudiment Size during Intestinal Regeneration in the Sea Cucumber, Holothuria glaberrima. Biomolecules 2019, 9, 873. [Google Scholar] [CrossRef] [Green Version]
  92. Girich, A.S.; Isaeva, M.P.; Dolmatov, I.Y. Wnt and frizzled expression during regeneration of internal organs in the holothurian Eupentacta fraudatrix. Wound Repair Regen. 2017, 25, 828–835. [Google Scholar] [CrossRef]
  93. Kawakami, Y.; Esteban, C.R.; Raya, M.; Kawakami, H.; Martí, M.; Dubova, I.; Belmonte, J.C.I. Wnt/β-catenin signaling regulates vertebrate limb regeneration. Genes Dev. 2006, 20, 3232–3237. [Google Scholar] [CrossRef] [Green Version]
  94. Novikova, E.L.; Bakalenko, N.I.; Nesterenko, A.Y.; Kulakova, M.A. Hox genes and animal regeneration. Russ. J. Dev. Biol. 2016, 47, 173–180. [Google Scholar] [CrossRef]
  95. Dolmatov, I.Y.; Kalacheva, N.V.; Tkacheva, E.S.; Shulga, A.P.; Zavalnaya, E.G.; Shamshurina, E.V.; Girich, A.S.; Boyko, A.V.; Eliseikina, M.G. Expression of Piwi, MMP, TIMP, and Sox during Gut Regeneration in Holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirotida). Genes 2021, 12, 1292. [Google Scholar] [CrossRef] [PubMed]
  96. Miao, T.; Wan, Z.; Sun, L.; Li, X.; Xing, L.; Bai, Y.; Wang, F.; Yang, H. Extracellular matrix remodeling and matrix metalloproteinases (ajMMP-2 like and ajMMP-16 like) characterization during intestine regeneration of sea cucumber Apostichopus japonicus. Comp. Biochem. Physiol. Part B Biochem. Mol. Biol. 2017, 212, 12–23. [Google Scholar] [CrossRef] [PubMed]
  97. Hernandez-Pasos, J.; Valentin-Tirado, G.; Garcia-Arraras, J.E. Melanotransferrin: New Homolog Genes and Their Differential Expression during Intestinal Regeneration in the Sea Cucumber Holothuria glaberrima. J. Exp. Zool. Part B Mol. Dev. Evol. 2017, 328, 259–274. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  98. Mashanov, V.S.; Zueva, O.R.; Garcia-Arraras, J.E. Expression of pluripotency factors in echinoderm regeneration. Cell Tissue Res. 2015, 359, 521–536. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  99. Dolmatov, I.Y.; Shulga, A.P.; Ginanova, T.T.; Eliseikina, M.G.; Lamash, N.E. Metalloproteinase inhibitor GM6001 delays regeneration in holothurians. Tissue Cell 2019, 59, 1–9. [Google Scholar] [CrossRef] [PubMed]
  100. Bello, S.A.; Torres-Gutierrez, V.; Rodriguez-Flores, E.J.; Toledo-Roman, E.J.; Rodriguez, N.; Diaz-Diaz, L.M.; Vazquez-Figueroa, L.D.; Cuesta, J.M.; Grillo-Alvarado, V.; Amador, A.; et al. Insights into intestinal regeneration signaling mechanisms. Dev. Biol 2019, 458, 12–31. [Google Scholar] [CrossRef]
  101. Bello, S.A.; Abreu-Irizarry, R.J.; Garcia-Arraras, J.E. Primary cell cultures of regenerating holothurian tissues. Methods Mol. Biol. 2015, 1189, 283–297. [Google Scholar]
  102. García-Arrarás, J.; Díaz-Miranda, L.; Torres, I.I.; File, S.; Jimenez, L.B.; Rivera-Bermudez, K.; Arroyo, E.J.; Cruz, W. Regeneration of the enteric nervous system in the sea cucumber Holothuria glaberrima. J. Comp. Neurol. 1999, 406, 461–475. [Google Scholar] [CrossRef]
  103. Tossas, K.; Qi-Huang, S.; Cuyar, E.; Garcia-Arraras, J.E. Temporal and spatial analysis of enteric nervous system regeneration in the sea cucumber Holothuria glaberrima. Regeneration 2014, 1, 10–26. [Google Scholar] [CrossRef] [PubMed]
  104. Siebert, S.; Farrell, J.A.; Cazet, J.F.; Abeykoon, Y.; Primack, A.S.; Schnitzler, C.E.; Juliano, C.E. Stem cell differentiation trajectories in Hydra resolved at single-cell resolution. Science 2019, 365, eaav9314. [Google Scholar] [CrossRef] [PubMed]
  105. Plass, M.; Solana, J.; Wolf, F.A.; Ayoub, S.; Misios, A.; Glazar, P.; Obermayer, B.; Theis, F.J.; Kocks, C.; Rajewsky, N. Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics. Science 2018, 360, eaaq1723. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  106. Gerber, T.; Murawala, P.; Knapp, D.; Masselink, W.; Schuez, M.; Hermann, S.; Gac-Santel, M.; Nowoshilow, S.; Kageyama, J.; Khattak, S.; et al. Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration. Science 2018, 362, eaaq0681. [Google Scholar] [CrossRef] [Green Version]
  107. Baek, S.; Tran, N.T.T.; Diaz, D.C.; Tsai, Y.Y.; Acedo, J.N.; Lush, M.E.; Piotrowski, T. Single-cell transcriptome analysis reveals three sequential phases of gene expression during zebrafish sensory hair cell regeneration. Dev. Cell 2022, 57, 799–819.e6. [Google Scholar] [CrossRef]
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Figure 1. Diagram of the intestinal regeneration pattern of sea cucumbers. (A) Structure of the normal sea cucumber digestive tract. (B) After evisceration. (C) Thickening of the mesentery and formation of a solid rod-shaped structure. (D) Lumen formation. (E) Differentiation and growth of the newborn intestine. Abbreviations: es, esophagus; st, stomach; fd, first descending intestine; dm, dorsal mesentery; lmu, longitudinal muscle; ai, ascending intestine; lm, lateral mesentery; sd, second descending intestine; vm, ventral mesentery; cl, cloaca; me, mesentery; ce, coelomic epithelium; oct, outer connective tissue; olm, outer longitudinal muscles; icm, inner circular muscles; mes, mesothelium; ict, inner connective tissue; le, luminal epithelium; srs, solid rod-shaped structure.
Figure 1. Diagram of the intestinal regeneration pattern of sea cucumbers. (A) Structure of the normal sea cucumber digestive tract. (B) After evisceration. (C) Thickening of the mesentery and formation of a solid rod-shaped structure. (D) Lumen formation. (E) Differentiation and growth of the newborn intestine. Abbreviations: es, esophagus; st, stomach; fd, first descending intestine; dm, dorsal mesentery; lmu, longitudinal muscle; ai, ascending intestine; lm, lateral mesentery; sd, second descending intestine; vm, ventral mesentery; cl, cloaca; me, mesentery; ce, coelomic epithelium; oct, outer connective tissue; olm, outer longitudinal muscles; icm, inner circular muscles; mes, mesothelium; ict, inner connective tissue; le, luminal epithelium; srs, solid rod-shaped structure.
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Table
Table 1. Patterns of Evisceration and Regeneration.
Table 1. Patterns of Evisceration and Regeneration.
SpeciesOrderEvisceratingRegeneratingReference
Autotomy SiteEvisceration SiteExcreted VisceraInitial Regeneration SiteRegeneration Cycle
Eupentacta quinquesemitaDendrochirotida(1) the oral body wall/the oral complex
(2) the longitudinal muscle/the retractor muscle
(3) intestine/mesentery
(4) intestine/cloaca		rupture of anterior end of the body (anterior)the oral complex; the digestive tract; part of the gonadsthe free end of the anterior mesentery2–3 weeks[33]
Eupentacta fraudatrix(1) rupture of the introvert
(2) the pharyngeal retractor muscles/the pharyngeal retractor muscles
(3) intestine/cloaca
(4) intestine/mesentery		the entire digestive tube; the oral complex free end of the dorsal mesentery at the healed oral end of the body; the free end of the ventral mesentery at the cloaca27 days[34]
Sclerodactyla briareus(1) the introvert and the muscular body wall
(2) the intestine/the cloaca
(3) intestine/mesentery		stomach; intestine; oral complexthe free end of the remaining mesentery20–37 days[35,36]
Holothuria glaberrimaHolothuriida Synallactida(1) the esophagus/the descending small intestine
(2) the large intestine/the cloaca
(3) intestine/mesentery		cloaca (posterior)the intestinal system; the hemal system; the right respiratory tree; most of the gonads28 days[23]
Apostichopus japonicus(1) the esophagus/the stomach
(2) the intestine/the cloaca
(3) intestine/mesentery		the intestinal system (except esophagus)the free end of the remaining mesentery; anlage arise from the esophagus; anlage arise from the cloaca21 days[37]
Holothuria polii(1) the esophagus/the stomach
(2) the intestine/the cloaca
(3) intestine/mesentery		the intestinal system (except pharynx and esophagus); the left respiratory tree; gonads6 weeks[38]
Table
Table 2. Single gene expression patterns during intestinal regeneration.
Table 2. Single gene expression patterns during intestinal regeneration.
Genes TypeGene NameGene Expression During RegenerationSpeciesExpression SitePutative FunctionProcess Involved inReference
MMPsEf-72 kDa type IV collagenase-Eupentacta fraudatrixcoelomic and luminal epitheliadegradation of ECM proteins and facilitating cell movementECM remodeling and cell migration[95]
Ef-MMP16-Eupentacta fraudatrixcoelomic epitheliummigration and/or proliferation of coelomic epithelial cellsmigration of coelomic epithelial cells[95]
ajMMP-16 likehigh expression during the whole process, with the highest expression at 1 dpe followed by a constant drop to normal level from 7 dpe to 21 dpeApostichopus japonicusno expression in normal intestine; in the regenerative intestine, expressed at coelomic and luminal epithelia degrading ECM and growth factors; targeting ECM components and biological moleculesECM remodeling[96]
ajMMP-2 likehighest expression at 6 hpe, slightly decreasing to approximately 2 at 1 dpe and 3 dpe, gradually declining to normal levelApostichopus japonicusno expression in normal intestine; in the regenerative intestine, expressed at luminal epithelia [96]
TIMPsEf-tensilin3-Eupentacta fraudatrixcoelomic epithelium and the ventral part of the luminal epithelium; opposite to EF-MMP16inhibiting the activity of MMPsECM remodeling [95]
immune-related geneSerum amyloid Ahigh expression during the whole process, with the highest expression at 15 dpeHolothuria glaberrimacoelomic epitheliumstimulate cell migration and adhesion to an ECM substrate; involved in the formation of luminal epithelium and muscular layer ECM remodeling; formation of luminal epithelium and the muscular layers [75]
Melanotransferrin (MTf)Hg MTf1/Aj MTfincreased expression from day 3, reaching the peak at 7 dpeHolothuria glaberrimamesotheliumimmune activationdedifferentiation of mesothelial cells and the following proliferation and migration [97]
Hg MTf2
Hg MTf3high expression at 3 dpe and 5 dpe, followed by decreasing gradually to normal level
Hg MTf4
Wnt signaling pathwayWntAup-regulated significantly from 6 h to day 14 with the maximum expression at 14 dpeApostichopus japonicusin the luminal epidermal, muscle layer, and submucosabe connected with cell proliferationthe proliferation, dedifferentiation and migration of luminal epithelium, muscle layer, and submucosa cells and apoptosis in the basal lamina of the mucosal epithelium at each stage of wound healing[83]
Wnt9strongest expression on days 7–14Holothuria glaberrimain normal intestine, no expression; in the regenerative intestine, expressed at mesotheliumcontrol the transitions between the dedifferentiated mesothelial cells and the mesenchymaa series of activities of the mesothelium[80]
gene involved in embryogenesisBmp1/Tllup-regulated on days 3 through 12Holothuria glaberrimain the apices of the developing folds of the luminal epithelial remodeling of extracellular matrixmorphogenetic movements leading to folding of the luminal epithelium and gut looping[80]
TCTPhighest expression at 3 dpe and 21 dpeHolothuria glaberrimain normal intestine, expressed at the apices of the luminal epithelial; in the regenerative intestine, expressed at mesothelium similar to survivin and mortalinapoptosis suppression and regulation cell proliferation deep transient dedifferentiation of mesothelial cells[80]
survivininsignificant increase at 7 dpe and 14 dpe, significant increase at 21 dpeHolothuria glaberrimain normal intestine, expressed at the base of the luminal epithelium; in the regenerative intestine, mostly expressed at mesotheliumcell proliferation; suppression of the programmed cell deathdedifferentiation of mesothelial cells and extensive proliferation[72]
mortalintwo peaks of roughly 3-fold up-regulation at 7 dpe and 21 dpeHolothuria glaberrimain normal and regenerative intestine, expressed at mesotheliumcell proliferation[72]
piwihigh expression during the whole process, with the highest expression at 3 dpeEupentacta fraudatrixin normal intestine, expressed at mesothelium; in the regenerative intestine, expressed at ECM-do not participate in the formation of the luminal epithelium[95]
Sox gene familyEf-Sox9/10 -Eupentacta fraudatrixcoelomic epithelium, mesenchymal cells, and the developing luminal epitheliumregulating the differentiation of mesenchymal cells into epithelial cellscoelomic epithelium transdifferentiation; the redifferentiation of myoepithelial cells and the formation of muscle layer[95]
Ef-Sox17-Eupentacta fraudatrixat the site of immersion only in surface cellsregulation of the initial stages of transdifferentiation[95]
pluripotency factors (Yamanaka factors)SoxB1down-regulation at the early post-evisceration stages (3 dpe and 7 dpe)Holothuria glaberrimain normal intestine, in the luminal epithelium; no signal at 3 dpe; expressed in the mesothelium at 7 dpe; finally expressed in the luminal epitheliumnot essential for cell dedifferentiation-[98]
MycUp-regulated at 3 dpe, but then returned to normal level, before being slightly (∼1.5 fold) down-regulated at 21 dpein normal intestine, in the luminal epithelium and mesothelium; expressed in the mesothelium at 3 dpe; expressed in the mesothelial epithelial cells at 7 dpe; finally expressed in the luminal epitheliumplay an important role in dedifferentiation/regenerationdedifferentiation of mesothelium[98]
Klf1/2/4 no significant differencesin normal intestine, in the luminal epithelium; expressed in the mesothelium at 3 dpe; expressed in the mesothelial epithelial cells at 7 dpe; finally expressed in the luminal epithelium-[98]
Oct 1/2/11-[98]
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Su, F.; Yang, H.; Sun, L. A Review of Histocytological Events and Molecular Mechanisms Involved in Intestine Regeneration in Holothurians. Biology 2022, 11, 1095. https://doi.org/10.3390/biology11081095

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Su F, Yang H, Sun L. A Review of Histocytological Events and Molecular Mechanisms Involved in Intestine Regeneration in Holothurians. Biology. 2022; 11(8):1095. https://doi.org/10.3390/biology11081095

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Su, Fang, Hongsheng Yang, and Lina Sun. 2022. "A Review of Histocytological Events and Molecular Mechanisms Involved in Intestine Regeneration in Holothurians" Biology 11, no. 8: 1095. https://doi.org/10.3390/biology11081095

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