Synthesis and Application of AgNPs-Chitosan Composite as a Self-Disinfecting Coating in Water-Based Polyurethane
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Bacterial Strain and Growth Condition
2.3. Antibacterial Activity Testing of AgNPs and Chitosan
2.4. Synthesis of AgNPs-Chitosan Composite
2.5. Physical and Chemical Characterization of AgNPs and AgNPs-Chitosan Composite
2.6. Preparation of Coating on Acrylic Piece as Sample Testing
2.7. Antibacterial Activity Testing of Self-Disinfecting Coating
2.8. Quantification of Silver Ion Releasing from Self-Disinfection Coating
2.9. Cytotoxicity Testing of AgNPs
2.10. Statistical Analysis
3. Results
3.1. Antibacterial Activity Testing of AgNPs and Chitosan
3.2. Physical and Chemical Characterization of AgNPs and AgNPs-Chitosan Composite
3.3. The Suitable Method of Self-Disinfection Coating Coated on Acrylic Surface
3.4. Short- and Long-Term Antibacterial Activity Testing of Self-Disinfection Coating
3.5. Quantification of Silver Ion Release from Self-Disinfecting Coating
3.6. Cytotoxicity Testing of AgNPs Solution
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Formula (F) | Conc. of AgNPs (µg/mL) | Conc. of Chitosan (µg/mL) | Percentage of Water-Based Polyurethane |
---|---|---|---|
F1 | 32.0 | 0.0 | 50% |
F2 | 128.0 | 0.0 | 50% |
F3 | 320.0 | 0.0 | 50% |
F4 | 1280.0 | 0.0 | 50% |
F5 | 32.0 | 39.0 | 50% |
F6 | 128.0 | 39.0 | 50% |
F7 | 320.0 | 39.0 | 50% |
F8 | 1280.0 | 39.0 | 50% |
Bacteria | MIC (µg/mL) | MBC (µg/mL) | ||
---|---|---|---|---|
AgNPs | Chitosan | AgNPs | Chitosan | |
Escherichia coli O157:H7 | 2.00 ± 0.00 | 156.00 ± 0.00 | 4.00 ± 0.00 | 260.00 ± 9.07 |
Vibrio cholera ATCC | 2.00 ± 0.00 | 78.00 ± 0.00 | 4.00 ± 0.00 | 78.00 ± 0.00 |
Staphylococcus aureus ATCC 25923 | 26.67 ± 9.24 | 156.00 ± 0.00 | 85.33 ± 6.95 | 208.00 ± 9.07 |
Methicillin-resistant S. aureus DMST20649 | 32.00 ± 0.00 | 208.00 ± 0.00 | 128.00 ± 0.00 | 208.00 ± 9.07 |
Coating Formulations | Average Values of Contact Angle (Degrees) | Average Values of Wetting Tension (dy/cm) |
---|---|---|
Uncoated (control) | 74.24 ± 3.25 | 19.75 ± 3.95 |
50% WPU | 70.76 ± 3.18 | 23.96 ± 3.97 |
Formula 1 | 71.84 ± 3.50 | 22.74 ± 3.65 |
Formula 2 | 72.29 ± 4.77 | 22.08 ± 5.77 |
Formula 3 | 67.10 ± 3.14 | 28.21 ± 4.04 |
Formula 4 | 83.22 ± 2.33 | 10.11 ± 1.71 |
Formula 5 | 67.51 ± 4.23 | 28.49 ± 1.05 |
Formula 6 | 68.65 ± 1.74 | 26.49 ± 2.07 |
Formula 7 | 66.93 ± 3.65 | 28.48 ± 4.35 |
Formula 8 | 81.73 ± 1.28 | 8.86 ± 1.62 |
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Siritongsuk, P.; Thammawithan, S.; Srichaiyapol, O.; Nasompag, S.; Pongha, S.; Daduang, S.; Klaynongsruang, S.; Patramanon, R. Synthesis and Application of AgNPs-Chitosan Composite as a Self-Disinfecting Coating in Water-Based Polyurethane. Coatings 2022, 12, 1832. https://doi.org/10.3390/coatings12121832
Siritongsuk P, Thammawithan S, Srichaiyapol O, Nasompag S, Pongha S, Daduang S, Klaynongsruang S, Patramanon R. Synthesis and Application of AgNPs-Chitosan Composite as a Self-Disinfecting Coating in Water-Based Polyurethane. Coatings. 2022; 12(12):1832. https://doi.org/10.3390/coatings12121832
Chicago/Turabian StyleSiritongsuk, Pawinee, Saengrawee Thammawithan, Oranee Srichaiyapol, Sawinee Nasompag, Sarawut Pongha, Sakda Daduang, Sompong Klaynongsruang, and Rina Patramanon. 2022. "Synthesis and Application of AgNPs-Chitosan Composite as a Self-Disinfecting Coating in Water-Based Polyurethane" Coatings 12, no. 12: 1832. https://doi.org/10.3390/coatings12121832