Ascorbate Supplementation: A Blessing in Disguise for Tomato Seedlings Exposed to NiO Nanoparticles
Round 1
Reviewer 1 Report
-Please use term AsA-supply instead of AsA-exposure in abstract and other parts of the manuscript.
-What does f.w. mean in all figures. f.w. should be mentioned/ elaborated in legends of all figures.
-Discussion section is too lengthy, and readers will be lost in the middle; hence, it needs sub-sections.
-Description regarding the role of AsA in the uptake (and translocation etc) is lacking. Its inclusion in the paper would be very interesting for the readers.
Author Response
Please see the attachment. The reviewer's comments are written in black, and the author's answers are written in blue.
Author Response File: Author Response.docx
Reviewer 2 Report
Nanomaterials offer wide-ranging solutions and their intensified use causes environmental contamination, posing ecotoxicological risks to several organisms, including plants. It becomes important to understand the phytotoxicity of NMs and find sustainable strategies to enhance plant tolerance to these emerging contaminants. Thus, this paper aimed to evaluate the potential of ascorbic acid (AsA) in increasing the tolerance of in vitro grown tomato seedlings to nickel oxide nanomaterials (nano-NiO). In general, the manuscript is well written and results are quite significant and I have only following minor comments:
Minor comments:
1. For the treatments, what are basis to choose the following concentrations: 30 m/L nango-NiO and 150 mg/L AsA. Authors should provide information for these treatments.
2. The authors measured the shoot and root length, how about the fresh weight of shoot and root, which should be also measured?
3. ROS is one of the most important factor for the stress, have you measured ROS content in this study?
4. For the keyword, normally, the first letter of the keyword should be capital.
5. For some references, year is blacked, but few of them are not, please make it all consistent.
Author Response
Please see the attachment. The reviewer's comments are written in black, and the author's answers are written in blue.
Author Response File: Author Response.docx
Round 2
Reviewer 1 Report
The authors have addressed all my comments/suggestions.